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Ling Y.-H.,Anhui Agricultural University | Xiang H.,Anhui Agricultural University | Xiang H.,Biobreeding Laboratory of Anhui Province | Li Y.-S.,Anhui Agricultural University | And 5 more authors.
Gene | Year: 2014

Fecundity improvement is one of the most important objectives for goat breeders as it greatly increases production efficiency. To investigate the genes associated with litter sizes in the Anhui White goat (AWG), gene expression differences in the ovaries of uniparous and multiparous AWG were assessed using the RNA-Seq (Quantification) method. This analysis generated 6,027,714 and 5,884,062 clean reads in uniparous and multiparous libraries, respectively. A total of 2201 differentially expressed genes (DEGs) were thereby identified (FDR≤0.001, |log2Ratio|≥1). There were 1583 up-regulated and 618 down-regulated genes in the multiparous samples compared with the uniparous samples. A large number of these DEGs were related to the terms cellular process, cell & cell part and binding. Twelve genes which may be associated with the high prolificacy of AWG were identified using a bioinformatic screen. In addition, pathway analysis revealed that these DEGs were significantly enriched in 11 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including ECM-receptor interactions, focal adhesion, and regulation of the actin cytoskeleton among others. This suggested a role for these pathways in the prolificacy of AWG. These results provide a list of new candidate genes for goat prolificacy. © 2014 Elsevier B.V. Source


Zhou Q.-Q.,Anhui Agricultural University | Zhou Q.-Q.,Biobreeding Laboratory of Anhui Province | Chen H.-Q.,Anhui Agricultural University | Chen H.-Q.,Biobreeding Laboratory of Anhui Province | And 5 more authors.
Progress in Biochemistry and Biophysics | Year: 2011

Thyroid hormone responsive spot 14 (THRSP) is a nucleoprotein induced by thyroid hormone, which makes an essential effect on the regulation of animal lipogenesis. In order to clarify the genetic mechanisms of the differences between Chinese native pig breeds (fat-type group, FTG) and introduced pig species (lean-type group, LTG) in carcass fat deposition, DNA samples of ear tissues were extracted from FTG(n = 228) composed of Wannan Spotted, Jixi Black and Dingyuan and LTG (n = 92) from Landrace, Large White, Duroc and their hybrid pigs and then detected the SNPs in the coding region of THRSP gene. The effects of SNPs on mRNA folding and protein secondary structure in FTPG and LTPG were predicted using Vienna RNA folding software and YASPIN web services, and their distribution and association with lipogenesis capability in FTG and LTG were analyzed. The results showed that the homologies of nucleotide sequence in coding region of pig THRSP gene with human and cattle were 86%and 88%, respectively. Two SNP sites, G123Aand A308G, were located at 123 bp and 308 bp away from the start of the CDS, respectively. The mutation at G123A site was a synonymous mutation, but the mutation at A308G site caused the change of amino acids from lysine to arginine at 103rd site of THRSP protein which resulted in the changes of casein kinase II phosphorylation site from TKEE to TREE, mRNA folding and protein secondary structure. The frequencies of alleles 123G and 308A were 0.975 9 and 0.589 9 in FTG, repectively, while that of alleles 123G and 308A were 0.657 7 and 0.815 2 in LTG. The total frequency of gamete 123G308A and 123G308G in FTG achieved 0.975 9, while that of gamete 123A308A and 123G308A in LTG was 0.815 3. Data from the present study imply the association of polymorphism at G123A and A308G sites with lipogenesis capability of pigs and their important regulating role in the expression of lipogenesis genes in pigs. Source


Ying-Hui L.,Anhui Agricultural University | Ying-Hui L.,Biobreeding Laboratory of Anhui Province | Li-Sheng W.,Anhui Agricultural University | Li-Sheng W.,Biobreeding Laboratory of Anhui Province | And 12 more authors.
Journal of Animal and Plant Sciences | Year: 2014

The genetic diversity of livestock breeds plays an important role in livestock production, but the significant loss of breeds is threatening genetic diversity of farm animal genetic resources (AnGR). The Weitzman approach which was accepted as a framework for assessment of genetic diversity on AnGR was exploited. In this study, Weitzman method was applied to set the priority of conservation by using genetic parameters of 12 Chinese meat goat breeds. Several measurement indexes of genetic diversity, involving total genetic diversity, contributions of each breed to the total diversity, marginal diversities, conservation potential, were calculated based on 11 microsatellite marker data of 12 Chinese meat goat breeds. The expected diversity of 12 goat breeds was 7132 which was 50.03% of the total genetic diversity. The mean extinction probability of 12 breeds was 0.375, where Hainan black goat was the highest, followed by Tibet goat, Haimen goat and Guizhou white goat. The marginal diversity of Hainan black goat in all breeds was the highest (-0.312), Fuqing goat was the lowest (-0.189). Hainan black goat had the greatest relative contribution to overall genetic diversity, followed by Maguan goat, Yichang white goat and Tibet goat. According to the trend of extinction, the conservation priorities of 12 breeds were Hainan black goat, Tibet goat, Guizhou white goat, Maguan goat, Chengdu ma goat, Haimen goat, Jianchang black goat, Anhui white goat, Fuqing goat, Yichang white goat, Shannan white goat and Matou goat. The Weitzman approach is a new idea to biodiversity resources protection, which is a reference for breed conservation and the decision-making of the relevant policy. We hope to provide objective and rational criteria and strategy for decision-making in conservation of Chinese meat goat breeds. Source


Ling Y.H.,Anhui Agricultural University | Ling Y.H.,Biobreeding Laboratory of Anhui Province | Ding J.P.,Anhui Agricultural University | Ding J.P.,Biobreeding Laboratory of Anhui Province | And 12 more authors.
Genetics and Molecular Research | Year: 2013

MicroRNAs (miRNAs) are an important class of small noncoding RNAs that are highly conserved in plants and animals. Many miRNAs are known to mediate a myriad of cell processes, including proliferation and differentiation, via the regulation of some transcription and signaling factors, which are closely related to muscle development and disease. In this study, small RNA cDNA libraries of Boer goats were constructed. In addition, we obtained the goat muscle miRNAs by using Solexa deep-sequencing technology and analyzed these miRNA characteristics by combining it with the bioinformatics technology. Based on Solexa sequencing and bioinformatics analysis, 562 species-conserved and 5 goat genome-specific miRNAs were identified, 322 of which exceeded 100 in the expression levels. The results of real-time quantitative polymerase chain reaction from 8 randomly selected miRNAs showed that the 8 miRNAs were expressed in goat muscle, and the expression patterns were consistent with the Solexa sequencing results. The identification and characterization of miRNAs in goat muscle provide important information on the role of miRNA regulation in muscle growth and development. These data will help to facilitate studies on the regulatory roles played by miRNAs during goat growth and development. © FUNPEC-RP. Source

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