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Wang J.,Northwest Normal University | Wang J.,Bioactive Products Engineering Research Center For Gansu Distinctive Plants | Niu S.,Northwest Normal University | Zhao B.,Northwest Normal University | And 5 more authors.
Carbohydrate Polymers | Year: 2014

Sulfated derivatives of Artemisia sphaerocephala polysaccharide (ASP) with high degree of substitution (DS) were synthesized using 4-dimethylaminopyridine (DMAP)/dimethylcyclohexylcarbodiimide (DCC) as catalyst. Size exclusion chromatography combined with multi-angle laser light scattering (SEC-LLS) results showed a decrease in fractal dimension (df) values of sulfated ASP (SASP). Compared to ASP and SASP with low DS (0.51-1.01), SASP cata2 exhibited an internal structure between rigid rod and random coil with a DS of 1.24. DS had greater influence on its conformation in aqueous solution. Circular dichroism (CD), methylene blue (MB) and Congo red (CR) spectrophotometric method and atomic force microscopy (AFM) results confirmed that the degradation of ASP and SO3H groups improved significantly the stiffness of the chains due to the electrostatic effect. Furthermore, antioxidant experiments revealed that high DS could enhance the scavenging activities of radicals and reducing power of SASP in vitro. The extended chain conformation was beneficial to enhance the biological activity of sulfated polysaccharides. © 2014 Published by Elsevier Ltd. All rights reserved. Source


Wang J.,Bioactive Products Engineering Research Center For Gansu Distinctive Plants | Wang J.,Northwest Normal University | Guo H.,Gansu Academy of Medical science | Zhang J.,Bioactive Products Engineering Research Center For Gansu Distinctive Plants | And 8 more authors.
Carbohydrate Polymers | Year: 2010

Sulfated polysaccharides exerted potent biological property which was relative to degree of sulfation (DS), Mw, substitution position and chain conformation. In the present study, sulfated derivatives of Artemisia sphaerocephala polysaccharide (ASP) with different DS were synthesized by chlorosulfuric acid/pyridine method. FT-IR and 13C NMR analysis indicated that C-6 substitution was predominant in sulfated ASP (SASP) compared with other positions at C-2. In the sulfation reaction, a sharp decrease in Mw was observed in SASP. The df values from 1.96 to 2.77 indicated that the -SO3H groups leading to the relatively expanded conformation of SASP. Antioxidant assays showed that SASP had better antioxidant activities. The data obtained in vitro models indicated that high DS and moderate Mw showed the best antioxidant capacities. Crown Copyright © 2010. Source


Wang F.,Northwest Normal University | Wang F.,Bioactive Products Engineering Research Center For Gansu Distinctive Plants | Liu Q.,Bioactive Products Engineering Research Center For Gansu Distinctive Plants | Liu Q.,Northwest Normal University | And 5 more authors.
International Journal of Biological Macromolecules | Year: 2016

As a great deal of interest is developed to study novel bioactive components with health benefit effects from natural resources, in this paper, a rat pheochromocytoma line 12 (PC12) cell is built to observe the protective effect of a Cynomorium songaricum Rupr. polysaccharide (CSP) against H2O2-induced oxidative stress. Fluorescence microscope, flow cytometry and micro-plate reader are used to assess cell viability and apoptosis. And the levels of reactive oxygen species (ROS), 8-hydroxy-2'-deoxyguanosine (8-OH-dG), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and lactate dehydrogenase (LDH) are evaluated. The results show that, the CSP can significantly protect PC12 cells against H2O2-induced oxidative stress, increase the intracellular antioxidase system load and inhibit H2O2-induced apoptosis by scavenging of ROS, regulating cell cycle, preventing DNA damage and protecting the cell membrane. This research would be benefit for preventing and curing the oxidation-related diseases in polysaccharide study. © 2016. Source


Wang J.,Northwest Normal University | Wang J.,Bioactive Products Engineering Research Center For Gansu Distinctive Plants | Zhao B.,Northwest Normal University | Zhao B.,Bioactive Products Engineering Research Center For Gansu Distinctive Plants | And 4 more authors.
Journal of the Taiwan Institute of Chemical Engineers | Year: 2012

Sulfated polysaccharides exert significant biological activity which is depend on degree of substitution (DS), weight average molecular weight (MW), substitution position and molecular conformation. In the present study, commercial guar gum was purified and its sulfated derivate (SGG) was prepared by Box-Behnken statistical design. Results of FT-IR and X-ray photoelectron spectroscopy (XPS) indicated that SO3 - groups were widely present in SGG molecules. A conformation transition from a compact chain conformation of branched clusters to a random coil conformation was observed in size exclusion chromatography (SEC) combined with laser light scattering (SEC-LLS) analysis. It could be attributed to a comprehensive effect of molecule weight, sulfo groups and galactose substitution pattern in the sulfated derivatives. © 2012. Source


Wang F.,Northwest Normal University | Wang F.,Bioactive Products Engineering Research Center For Gansu Distinctive Plants | Wang W.,Northwest Normal University | Huang Y.,Northwest Normal University | And 5 more authors.
Food Hydrocolloids | Year: 2015

Cynomorium songaricum Rupr. (C. songaricum, Cynomoriaceae) is a food for local people and also a tonic in traditional Chinese medicine. As polysaccharide is an active component in it, it is great significant to study the structure. This research studied a new polysaccharide fraction purified from C. songaricum, named CSP-DS1 (Mw of 48.1 × 104 Da). The CSP-DS1 was investigated by chemical and instrumental analysis, including acid hydrolysis and methylation analysis, infrared spectra (IR), gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR) (1H, 13C, 1H-1H COSY, HMBC and HMQC). The results demonstrate that the CSP-DS1 is comprised of three kinds of monosaccharide: Man, Glc and Gal, in approximately mole ratios of 5.01: 89.17: 5.82. Based on characterization and analysis, its possible glucosidic linkage of the CSP-DS1 is also established. © 2015 Elsevier Ltd. Source

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