Bioactive Products Engineering Research Center for Gansu Distinctive Plants

Lanzhou, China

Bioactive Products Engineering Research Center for Gansu Distinctive Plants

Lanzhou, China
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Wang J.,Bioactive Products Engineering Research Center For Gansu Distinctive Plants | Wang J.,Northwest Normal University | Guo H.,Gansu Academy of Medical science | Zhang J.,Bioactive Products Engineering Research Center For Gansu Distinctive Plants | And 8 more authors.
Carbohydrate Polymers | Year: 2010

Sulfated polysaccharides exerted potent biological property which was relative to degree of sulfation (DS), Mw, substitution position and chain conformation. In the present study, sulfated derivatives of Artemisia sphaerocephala polysaccharide (ASP) with different DS were synthesized by chlorosulfuric acid/pyridine method. FT-IR and 13C NMR analysis indicated that C-6 substitution was predominant in sulfated ASP (SASP) compared with other positions at C-2. In the sulfation reaction, a sharp decrease in Mw was observed in SASP. The df values from 1.96 to 2.77 indicated that the -SO3H groups leading to the relatively expanded conformation of SASP. Antioxidant assays showed that SASP had better antioxidant activities. The data obtained in vitro models indicated that high DS and moderate Mw showed the best antioxidant capacities. Crown Copyright © 2010.


Wang J.,Northwest Normal University | Wang J.,Bioactive Products Engineering Research Center For Gansu Distinctive Plants | Zhao B.,Northwest Normal University | Zhao B.,Bioactive Products Engineering Research Center For Gansu Distinctive Plants | And 4 more authors.
Journal of the Taiwan Institute of Chemical Engineers | Year: 2012

Sulfated polysaccharides exert significant biological activity which is depend on degree of substitution (DS), weight average molecular weight (MW), substitution position and molecular conformation. In the present study, commercial guar gum was purified and its sulfated derivate (SGG) was prepared by Box-Behnken statistical design. Results of FT-IR and X-ray photoelectron spectroscopy (XPS) indicated that SO3 - groups were widely present in SGG molecules. A conformation transition from a compact chain conformation of branched clusters to a random coil conformation was observed in size exclusion chromatography (SEC) combined with laser light scattering (SEC-LLS) analysis. It could be attributed to a comprehensive effect of molecule weight, sulfo groups and galactose substitution pattern in the sulfated derivatives. © 2012.


Wang J.,Northwest Normal University | Wang J.,Bioactive Products Engineering Research Center For Gansu Distinctive Plants | Zhao B.,Northwest Normal University | Zhao B.,Bioactive Products Engineering Research Center For Gansu Distinctive Plants | And 6 more authors.
International Journal of Biological Macromolecules | Year: 2012

Selenium (Se) is an essential trace element for human beings and many other forms of life. Organic Se from natural foods has higher bioavailability and is safer than inorganic Se species. In the present study, selenium-containing derivatives of Artemisia sphaerocephala polysaccharide with the highest Se content of 1703μg/g were synthesized using H2SeO3/HNO3 and BaCl2 as a catalyst. The effects of the ratio of HNO3 concentration and reaction temperature were investigated. Results of Raman and 13C NMR spectroscopy indicated that C-6 substitution was predominant in selenized polysaccharide. A sharp decrease in Mw was observed in the selenized reaction due to the acid environment. The in vitro antioxidant results suggest that selenium-containing polysaccharide should be applied as a novel selenium source in dietary supplements, with potent antioxidant properties. © 2012.


Wang F.,Northwest Normal University | Wang F.,Bioactive Products Engineering Research Center for Gansu Distinctive Plants | Liu Q.,Bioactive Products Engineering Research Center for Gansu Distinctive Plants | Liu Q.,Northwest Normal University | And 5 more authors.
International Journal of Biological Macromolecules | Year: 2016

As a great deal of interest is developed to study novel bioactive components with health benefit effects from natural resources, in this paper, a rat pheochromocytoma line 12 (PC12) cell is built to observe the protective effect of a Cynomorium songaricum Rupr. polysaccharide (CSP) against H2O2-induced oxidative stress. Fluorescence microscope, flow cytometry and micro-plate reader are used to assess cell viability and apoptosis. And the levels of reactive oxygen species (ROS), 8-hydroxy-2'-deoxyguanosine (8-OH-dG), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and lactate dehydrogenase (LDH) are evaluated. The results show that, the CSP can significantly protect PC12 cells against H2O2-induced oxidative stress, increase the intracellular antioxidase system load and inhibit H2O2-induced apoptosis by scavenging of ROS, regulating cell cycle, preventing DNA damage and protecting the cell membrane. This research would be benefit for preventing and curing the oxidation-related diseases in polysaccharide study. © 2016.


Wang J.,Northwest Normal University | Wang J.,Bioactive Products Engineering Research Center for Gansu Distinctive Plants | Niu S.,Northwest Normal University | Zhao B.,Northwest Normal University | And 5 more authors.
Carbohydrate Polymers | Year: 2014

Sulfated derivatives of Artemisia sphaerocephala polysaccharide (ASP) with high degree of substitution (DS) were synthesized using 4-dimethylaminopyridine (DMAP)/dimethylcyclohexylcarbodiimide (DCC) as catalyst. Size exclusion chromatography combined with multi-angle laser light scattering (SEC-LLS) results showed a decrease in fractal dimension (df) values of sulfated ASP (SASP). Compared to ASP and SASP with low DS (0.51-1.01), SASP cata2 exhibited an internal structure between rigid rod and random coil with a DS of 1.24. DS had greater influence on its conformation in aqueous solution. Circular dichroism (CD), methylene blue (MB) and Congo red (CR) spectrophotometric method and atomic force microscopy (AFM) results confirmed that the degradation of ASP and SO3H groups improved significantly the stiffness of the chains due to the electrostatic effect. Furthermore, antioxidant experiments revealed that high DS could enhance the scavenging activities of radicals and reducing power of SASP in vitro. The extended chain conformation was beneficial to enhance the biological activity of sulfated polysaccharides. © 2014 Published by Elsevier Ltd. All rights reserved.


Wang F.,Northwest Normal University | Wang F.,Bioactive Products Engineering Research Center for Gansu Distinctive Plants | Wang W.,Northwest Normal University | Huang Y.,Northwest Normal University | And 5 more authors.
Food Hydrocolloids | Year: 2015

Cynomorium songaricum Rupr. (C. songaricum, Cynomoriaceae) is a food for local people and also a tonic in traditional Chinese medicine. As polysaccharide is an active component in it, it is great significant to study the structure. This research studied a new polysaccharide fraction purified from C. songaricum, named CSP-DS1 (Mw of 48.1 × 104 Da). The CSP-DS1 was investigated by chemical and instrumental analysis, including acid hydrolysis and methylation analysis, infrared spectra (IR), gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR) (1H, 13C, 1H-1H COSY, HMBC and HMQC). The results demonstrate that the CSP-DS1 is comprised of three kinds of monosaccharide: Man, Glc and Gal, in approximately mole ratios of 5.01: 89.17: 5.82. Based on characterization and analysis, its possible glucosidic linkage of the CSP-DS1 is also established. © 2015 Elsevier Ltd.


Wang J.,Northwest Normal University | Wang J.,Bioactive Products Engineering Research Center for Gansu Distinctive Plants | Niu S.,Northwest Normal University | Zhao B.,Northwest Normal University | And 9 more authors.
International Journal of Biological Macromolecules | Year: 2013

We reported here a new synthesis of C-2 and C-3 sulfated guar gum (SRSGG) with low degree of substitution (DSS) of 0.58, employing triphenylchloromethane (TrCl) as a protected precursor. The yield and DSTr (calculated from the weight of triphenylmethanol) of triphenylmethylated GG (GGTr) was 165.6% and 0.71, respectively. In addition, low ratio (1:4) of chlorosulfuric acid to pyridine (1:4) was chosen in sulfation reaction since the protecting group was slightly sensitive to acid. Results of FT-IR and 13C NMR spectroscopy indicated that C-2 and C-3 substitution was predominant but not fully sulfated in SRSGG. Size-exclusion chromatograph combined with multi-angle laser photometer (SEC-LLS) showed a decrease in molecular weight in the reaction. This might be due to the degradation in sulfation and deprotection process. Finally, we investigated the effect of structure features on the antioxidant activities in vitro. Vitro antioxidant experiments revealed that the regioselective sulfation at C-2 and C-3 and low molecular weight afforded strong antioxidant activities showing a much higher scavenging abilities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical than that by the known C-6-sulfated derivative. The antioxidant activities of sulfated polysaccharides were not a function of a single factor but a combination of molecular weight, DSS and substitution positions. © 2013.


Niu S.,Northwest Normal University | Niu S.,Bioactive Products Engineering Research Center for Gansu Distinctive Plants | Wang J.,Northwest Normal University | Wang J.,Bioactive Products Engineering Research Center for Gansu Distinctive Plants | And 10 more authors.
International Journal of Biological Macromolecules | Year: 2013

We reported here a regioselective synthesis of non-C-6 phosphorylated guar gum (PRSGG) with a degree of substitution (DS) 0.34. Triphenylchloromethane (TrCl) was employed as a key protected precursor. Detritylation was performed by treatment with an excess of dichloroacetic acid. Phosphorylated GG was successfully characterized by FT-IR and 13C NMR spectroscopies. It was indicated that C-2 and C-3 substitution was predominant in phosphorylated polysaccharide. A decrease in weight average molar mass (Mw) was observed in the reaction due to the acid environment in deprotection procedure. The results of vitro antioxidant experiments revealed that regioselective phosphorylation at C-2/C-3 and low Mw afforded strong antioxidant activities. Non-C-6 phosphorylated guar gum showed a much higher scavenging abilities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical than C-6-phosphorylated derivative. © 2013.


Wang J.,Bioactive Products Engineering Research Center for Gansu Distinctive Plants | Wang J.,Northwest Normal University | Zhang J.,Bioactive Products Engineering Research Center for Gansu Distinctive Plants | Zhang J.,Northwest Normal University | And 7 more authors.
Food Chemistry | Year: 2010

A water-soluble polysaccharide isolated from Cynomorium songaricum which is a traditional Chinese food was purified by Ultrahydrogel 500 gel filtration chromatography. The structural characterisation and hypoglycaemic activity of the purified polysaccharide fraction (CSPA) were evaluated. CSPA (Mw of 1.394 × 105 Da) was a heteropolysaccharide consisting of Ara, Glu and Gal. The conformation of CSPA was characterised as a compact chain of sphere-like structure in aqueous solution with a df value of 2.86. Linkage analysis and NMR (1D and 2D) revealed the presence of the following unit:→ 3) - α - araf - (1 → 3) - α - d - glcp - (1 → 4) - α - d -Gal p A6Me- (1 →. Pharmaceutical experiments showed that the administration of CSPA could significantly decrease blood glucose levels, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, blood urea nitrogen, creatinine activities, and effectively increase serum insulin level and the content of liver glycogen in streptozotocin-induced diabetic rats. Histopathology studies showed that the reduced islet cells were restored to near normal upon treatment with CSPA. The results suggest CSPA could be considered as a potential candidate for developing a new anti-diabetic agent. Crown Copyright © 2009.


Xu Y.,Northwest Normal University | Xu Y.,Bioactive Products Engineering Research Center for Gansu Distinctive Plants | Song S.,Northwest Normal University | Wei Y.,Northwest Normal University | And 8 more authors.
International Journal of Biological Macromolecules | Year: 2016

Sphallerocarpus gracilis (S. gracilis) is a little-investigated edible plant and used as traditional Chinese medicine. In this study, polysaccharide extracted from S. gracilis,deproteined and purified. The polysaccharide (SGP) was chemically modified to obtain its sulfated derivatives (S-SGP) using the method of chlorosulfonic acid/pyridine (CSA/Pyr). In order to acquire the derivative with the highest degree of substitution (DS), the optimum conditions of the sulfation were obtained based on response surface design (RSD), and the structural characterizations and antioxidant properties of the S-SGP were comparatively investigated by Fourier transform infrared spectrometry (FT-IR), GC-MS analysis, size exclusion chromatography (SEC), and DPPH radical assay, hydroxyl radical assay, superoxide radical assay, and reducing power assay, respectively. Results showed that the modification was successful, and obtained the optimum combination of conditions. Compared with SGP, the sulfated polysaccharide with relatively the decreased degree of molecular weight (Mw) but the same composition of monosaccharides exhibited better antioxidant activities in DPPH, hydroxyl, superoxide radical and reducing power assay. These results indicated that the antioxidant activities in vitro of the S-SGP from S. gracilis may be related to combined effects of Mw, monosaccharide composition, and sulfate content. © 2016.

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