Ibaraki, Japan
Ibaraki, Japan

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Shimokawa T.,Japan Forestry and Forest Products Research Institute | Shibuya H.,Japan Forestry and Forest Products Research Institute | Ikeda T.,Japan Forestry and Forest Products Research Institute | Magara K.,Japan Forestry and Forest Products Research Institute | And 4 more authors.
Journal of Wood Science | Year: 2013

The process of solid-state fermentation was used to produce a cocktail enzyme of Trichoderma reesei ATCC 66587 and Aspergillus tubingensis KRCF 700-33. Wheat bran, corncob, and sugi pulp were supplemented with ammonium sulfate as an enzyme-producing medium using T. reesei and A. tubingensis. The corncob blend ratio, duration of incubation, and ammonium sulfate concentration were optimized for enhancing cellulase production from T. reesei using a Box-Behnken design. Filter paper degrading activity more than tripled when T. reesei was grown in the optimized medium, as compared with the initial medium. The highest activity of 4.03 FPU/ml (about 29 FPU/g of dry material) was obtained with a cocktail enzyme having a 25 % content of A. tubingensis and 75 % of T. reesei. The sugi pulp was then fermented to ethanol with the cocktail enzyme and thermotolerant yeast (Saccharomyces cerevisiae BA-11) under simultaneous saccharification and fermentation (SSF) at 40 °C. An ethanol concentration of 4.48 % (w/v) was achieved using the cocktail enzyme (4 FPU/g-pulp) that was produced on-site with a substrate loading level of 12.5 wt %, which achieved an ethanol yield of 76 % after 72 h. © 2012 The Japan Wood Research Society.


Shimokawa T.,Japan Forestry and Forest Products Research Institute | Ikeda T.,Japan Forestry and Forest Products Research Institute | Magara K.,Japan Forestry and Forest Products Research Institute | Shinagawa S.,BioAcademia Inc. | And 3 more authors.
Nihon Enerugi Gakkaishi/Journal of the Japan Institute of Energy | Year: 2013

Bamboo is an attractive biomass resource which grows rapidly, reaching their maximum size within a few months. Bamboo pulp, prepared by soda-anthraquinone cooking, was fermented to ethanol with a cocktail enzyme of Aspergillus tubingensis KRCF7OO-33Y and Trichoderma reesei ATCC66587, and thermotolerant yeast (Saccharomyces cerevisiae BA-11) under simultaneous saccharification and fermentation (SSF) at 4O°C. The strain A. tubingensis KRCF700-33Y, having sporeless, high β-glucosidase productive capacity was generated by UV irradiation. The highest activity of 5 FPU/ml was obtained with a cocktail enzyme having a 10% content of A. tubingensis, and the cocktail effect was also shown under the SSF process. An ethanol concentration of 47.1 g/ L was achieved using a cocktail enzyme (15% content of A tubingensis) with a substrate loading level of 15% under small scale SSF, which resulted in an ethanol yield of 76.8% after 96 h. An ethanol concentration of bamboo pulp reached 44.0 g/L under SSF process using a reactor (1-L kneader) with a cocktail enzyme (10% content of A. tubingensis, 4 FPU/g-DM), which resulted in an ethanol yield of 71.8% after 144 h. The results indicated the feasibility of bioethanol production from bamboo pulp under SSF using on-site produced enzyme by solid-state fermentation.

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