Bio Science Research Center

Vishākhapatnam, India

Bio Science Research Center

Vishākhapatnam, India
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Das U.N.,UND Life science | Das U.N.,Jawaharlal Nehru Technological University Kakinada | Das U.N.,Bio Science Research Center
Prostaglandins Leukotrienes and Essential Fatty Acids | Year: 2012

Radiation induces overexpression and activity of the MET oncogene that, in turn, enhances the production of prostaglandin E 2, a pro-inflammatory molecule. Prostaglandin E 2 promotes tumor cell invasion, prevents apoptosis, enhances their metastasis and causes radioresistance. It is proposed that lipoxin A 4, a potent endogenous anti-inflammatory molecule, opposes the actions of prostaglandin E 2 and thus, could promote radiosensitivity, suppress tumor cell proliferation, invasiveness and suppress metastasis. Thus, methods designed to enhance endogenous lipoxin A 4 formation or its synthetic analogs may be useful in the management of cancer. © 2011 Elsevier Ltd.


Das U.N.,UND Life science | Das U.N.,Jawaharlal Nehru Technological University Kakinada | Das U.N.,Krishna Institute of Medical science | Das U.N.,Bio Science Research Center
Lipids in Health and Disease | Year: 2011

Inflammatory events persist in systemic lupus erythematosus (lupus) despite the use of anti-inflammatory (both steroidal and non-steroidal) and immunosuppressive drugs leading to delay in the healing/repair process and so tissue/organ damage continues. The continuation of inflammation in lupus could be attributed to failure of the resolution process due to deficiency of potent endogenous pro-resolution-inducing molecules such as lipoxin A 4(LXA4). It is likely that progression and flares of lupus and lupus nephritis are due to decreased formation and release of LXA 4. Hence, administration of LXA4and its analogues could be of benefit in lupus. Furthermore, plasma and urinary measurement of lipoxins may be used to predict prognosis and response to therapy. It is likely that lipoxins and other bioactive anti-inflammatory lipids such as resolvins, protectins, maresins and nitrolipids play a significant role in other auto-immune diseases such as rheumatoid arthritis, type 1 diabetes mellitus and multiple sclerosis and hence, could be of significant benefit in these diseases. © 2011 Das; licensee BioMed Central Ltd.


Das U.N.,Jawaharlal Nehru Technological University Kakinada | Das U.N.,Bio Science Research Center
Nutrition | Year: 2013

Diabetic macular edema and retinopathy are low-grade inflammatory conditions. Infusions of antitumor necrosis factor-α (anti-TNF-α) antibody and antivascular endothelial growth factor (anti-VEGF) antibody have been shown to be at least partly effective in the treatment of diabetic macular edema and proliferative diabetic retinopathy. Intravitreal therapy of diabetic macular edema by the anti-TNF-α antibody has been found to produce significant side effects and anti-VEGF therapy to be ineffective. Nevertheless, these studies have indicated that the suppression of TNF-α and other proinflammatory cytokines and VEGF could be of benefit in diabetic macular edema and retinopathy. The retina is rich in polyunsaturated fatty acids, especially in ω-3, and several studies have shown that polyunsaturated fatty acids prevent diabetic retinopathy. Lipoxins, resolvins, and protectins derived from various polyunsaturated fatty acids possess anti-inflammatory actions and suppress the production of interleukin-6, and TNF-α and VEGF have antiangiogenic actions. In view of these evidences, I propose that lipoxins, resolvins, and protectins could be of significant benefit in the prevention and management of diabetic macular edema and retinopathy. © 2013 Elsevier Inc.


Das U.N.,UND Life science | Das U.N.,Jawaharlal Nehru Technological University Kakinada | Das U.N.,Bio Science Research Center
Clinical Lipidology | Year: 2013

Obesity, insulin resistance, essential hypertension, Type 2 diabetes, nonalcoholic fatty liver disease, coronary heart disease, osteoporosis, renal failure, Alzheimer's disease, depression, schizophrenia, aging and rheumatological conditions are low-grade systemic inflammatory conditions, in which there is an increase in the production of proinflammatory cytokines, reactive oxygen species, reactive nitrogen species and proinflammatory eicosanoids, while a decrease in the cellular antioxidants, anti-inflammatory cytokines and certain polyunsaturated fatty acids, and their anti-inflammatory products, lipoxins (LXs), resolvins (Rvs), protectins, maresins and nitrolipids, occurs. This imbalance between the pro- and anti-inflammatory molecules in these diseases suggests that therapeutic strategies directed to suppress inappropriate inflammation and enhance the synthesis/action of anti-inflammatory bioactive lipids may aid the prevention of and recovery from these diseases. It is proposed that both local and systemic delivery of LXs, Rvs, protectins, maresins and nitrolipids and/or their more stable synthetic analogs may prove to be useful in these diseases. In this part II of the review, the discussion is centered on the role of LXs, Rvs, protectins and nitrolipids in diabetes mellitus and other diseases. © 2013 Future Medicine Ltd.


Das U.N.,UND Life science | Das U.N.,Jawaharlal Nehru Technological University Kakinada | Das U.N.,Bio Science Research Center
Clinical Lipidology | Year: 2013

Obesity, insulin resistance, essential hypertension, Type 2 diabetes, nonalcoholic fatty liver disease, coronary heart disease, osteoporosis, renal failure, Alzheimer's disease, depression, schizophrenia, aging, rheumatological conditions and cancer are low-grade systemic inflammatory conditions in which there is an increase in proinflammatory cytokines and reactive oxygen species, reactive nitrogen species, and proinflammatory eicosanoids, while a decrease in the cellular antioxidants, anti-inflammatory cytokines and certain polyunsaturated fatty acids, and their anti-inflammatory products such as lipoxins (LXs), resolvins (Rvs), protectins, maresins and nitrolipids occurs. This imbalance between the pro- and anti-inflammatory molecules in these diseases suggests that therapeutic strategies directed to suppress inappropriate inflammation may aid recovery from these diseases. It is proposed that both local and systemic delivery of LXs, Rvs, protectins, maresins and nitrolipids, and/or their more stable synthetic analogs, may prove to be useful in these diseases. In this part I of the review, the discussion is centered on the role of LXs, Rvs, protectins and nitrolipids in cancer. © 2013 Future Medicine Ltd.


Das U.N.,Jawaharlal Nehru Technological University Kakinada | Das U.N.,Bio Science Research Center | Madhavi N.,UND Life science
Lipids in Health and Disease | Year: 2011

Previous studies showed that -linolenic acid (GLA, 18: 3 -6), arachidonic acid (AA, 20:4 -6), eicosapentaenoic acid (EPA, 20: 5 -3) and docosahexaenoic acid (DHA, 22:6 -3) have selective tumoricidal action. In the present study, it was observed that dihomo-gamma-linolenic acid (DGLA) and AA, EPA and DHA have cytotoxic action on both vincristine-sensitive (KB-3-1) and resistant (KB-ChR-8-5) cancer cells in vitro that appeared to be a free-radical dependent process but not due to the formation of prostaglandins, leukotrienes and thromboxanes. Uptake of vincristine and fatty acids was higher while their efflux was lower in KB-3-1 cells compared with KB-ChR-8-5 cells, suggesting that drug resistant cells have an effective efflux pump. GLA, DGLA, AA, EPA and DHA enhanced the uptake and decreased efflux in both drug-sensitive and drug-resistant cells and augmented the susceptibility of tumor cells especially, of drug-resistant cells to the cytotoxic action of vincristine. These results suggest that certain polyunsaturated fatty acids have tumoricidal action and are capable of enhancing the cytotoxic action of anti-cancer drugs specifically, on drug-resistant cells by enhancing drug uptake and reducing its efflux. Thus, polyunsaturated fatty acids either by themselves or in combination with chemotherapeutic drugs have the potential as anti-cancer molecules. © 2011 Das and Madhavi; licensee BioMed Central Ltd.


Das U.N.,UND Life science | Das U.N.,Jawaharlal Nehru Technological University Kakinada | Das U.N.,Bio Science Research Center
Nutrition | Year: 2012

The inflammatory process seen in multiple sclerosis is due to an excess production of proinflammatory cytokines interleukin-1 (IL-1), IL-6, tumor necrosis factor-α, interferons, macrophage migration inhibitory factor, HMGB1 (high mobility group B1), and, possibly, a reduction in antiinflammatory cytokines IL-10, IL-4, and transforming growth factor-β that leads to increased secretion of reactive oxygen species, including nitric oxide, resulting in neuronal damage. It is suggested that failure of production of adequate amounts of resolution-inducing molecules lipoxins, resolvins, and protectins that suppress inflammation and reactive oxygen species production, enhance wound healing, and have neuroprotective properties results in inappropriate inflammation and delay in the healing/repair process, and so neuronal damage continues, as seen in multiple sclerosis. Hence, methods designed to enhance the production and/or administration of lipoxins, resolvins, and protectins may form a new approach in the prevention and treatment of multiple sclerosis and other similar autoimmune diseases. © 2012 Elsevier Inc.


Das U.N.,UND Life science | Das U.N.,Bio Science Research Center | Das U.N.,Jawaharlal Nehru Technological University Kakinada
Critical Care | Year: 2013

The usefulness of n-3 fatty acids, γ-linolenic acid and antioxidants in the critically ill is controversial. I propose that adverse outcome in the critically ill is due to excess production of proinflammatory cytokines and eicosanoids from polyunsaturated fatty acids (PUFAs), while generation of anti-inflammatory products of PUFAs may lead to a favorable outcome. Hence, I suggest that measurement of plasma levels of various cytokines, free radicals, and proinflammatory and anti-inflammatory products of PUFAs and correlating them to the clinical picture may pave the way to identify prognostic markers and develop newer therapeutic strategies to prevent and manage critical illness. © 2013 BioMed Central Ltd.


Das U.N.,UND Life science | Das U.N.,Bio Science Research Center | Das U.N.,Jawaharlal Nehru Technological University Kakinada
Critical Care | Year: 2013

Sepsis due to unabated inflammation is common. Increased production of pro-inflammatory cytokines, free radicals, and eicosanoids has been detected in sepsis and other critical illnesses but could also be due to decreased synthesis and release of anti-inflammatory molecules. Increased serum adipose-fatty acid-binding protein (A-FABP) levels can cause insulin resistance and have been reported in the critically ill, serve as a marker of prognosis, and thus link metabolic homeostasis and inflammation. A-FABP can be linked to the expression of Toll-like receptors, macrophage activation, synthesis and release of pro-inflammatory cytokines interleukin-6 and tumor necrosis factor-alpha, activation of cyclooxygenase 2 (COX-2) expression, and eicosanoid synthesis, events that can cause insulin resistance and initiation and progression of inflammation and sepsis. Unsaturated fatty acids and their anti-inflammatory products, such as lipoxins, resolvins, and protectins, may suppress A-FABP expression, inhibit macrophage and COX-2 activation, and decrease production of pro-inflammatory cytokines and ultimately could lead to a decrease in insulin resistance and resolution of inflammation and recovery from sepsis. Serial measurement of these pro- and anti-inflammatory molecules and correlation of their levels to the progression to or recovery from (or both) sepsis and other inflammatory processes may form a new approach to predict prognosis in inflammatory conditions and eventually could lead to the development of new therapeutic strategies. © 2013 BioMed Central Ltd.


Bathina S.,Bio Science Research Center | Das U.N.,Bio Science Research Center
Archives of Medical Science | Year: 2015

Brain-derived neurotrophic factor (BDNF) plays an important role in neuronal survival and growth, serves as a neurotransmitter modulator, and participates in neuronal plasticity, which is essential for learning and memory. It is widely expressed in the CNS, gut and other tissues. BDNF binds to its high affinity receptor TrkB (tyrosine kinase B) and activates signal transduction cascades (IRS1/2, PI3K, Akt), crucial for CREB and CBP production, that encode proteins involved in β cell survival. BDNF and insulin-like growth factor-1 have similar downstream signaling mechanisms incorporating both p-CAMK and MAPK that increase the expression of pro-survival genes. Brain-derived neurotrophic factor regulates glucose and energy metabolism and prevents exhaustion of β cells. Decreased levels of BDNF are associated with neurodegenerative diseases with neuronal loss, such as Parkinson's disease, Alzheimer's disease, multiple sclerosis and Huntington's disease. Thus, BDNF may be useful in the prevention and management of several diseases including diabetes mellitus. © Copyright 2015 Termedia & Banach.

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