BIO FD and C Co.

Incheon, South Korea

BIO FD and C Co.

Incheon, South Korea

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Baque M.A.,Chungbuk National University | Baque M.A.,Sher-e-Bangla Agricultural University | Moh S.-H.,BIO FD and C Co. | Lee E.-J.,Chungbuk National University | And 2 more authors.
Biotechnology Advances | Year: 2012

The increasing global demand for biomass of medicinal plant resources reflects the issues and crisis created by diminishing renewable resources and increasing consumer populations. Moreover, diverse usage of plants and reduced land for cultivation in the world accelerated the deficiency of plant resources. In addition, the preparation of safety of plant based medicine whips up demand for biomass of valuable medicinal plants. As one of alternative approach to upswing the productivity of plant-based pharmaceutical compounds, automation of adventitious root culture system in air-lift bioreactor was adopted to produce cosmic amount of root biomass along with enriched diverse bioactive molecules. In this review, various physiological, engineering parameters, and selection of proper cultivation strategy (fed-batch, two-stage etc.) affecting the biomass production and secondary metabolite accumulation have been discussed. In addition, advances in adventitious root cultures including factors for process scale-up as well as recent research aimed at maximizing automation of the bioreactor production processes are also highlighted. Examples of the scale-up of cultures of adventitious roots of Morinda citrifolia, Echinacea purpurea and angustifolia, Hypericum perforatum and Panax ginseng by applying 20. L to 10, 000. L bioreactors in our lab were demonstrated with a view of commercial application. © 2012 Elsevier Inc.


Seo H.-H.,BIO FD and C Co. | Park S.,Chonnam National University | Park S.,Experiment Research Institute | Oh B.-J.,Biological Control Center | And 4 more authors.
PLoS ONE | Year: 2014

Functional characterization of a defensin, J1-1, was conducted to evaluate its biotechnological potentiality in transgenic pepper plants against the causal agent of anthracnose disease, Colletotrichum gloeosporioides. To determine antifungal activity, J1-1 recombinant protein was generated and tested for the activity against C. gloeosporioides, resulting in 50% inhibition of fungal growth at a protein concentration of 0.1 mg·mL-1. To develop transgenic pepper plants resistant to anthracnose disease, J1-1 cDNA under the control of 35S promoter was introduced into pepper via Agrobacterium-mediated genetic transformation method. Southern and Northern blot analyses confirmed that a single copy of the transgene in selected transgenic plants was normally expressed and also stably transmitted to subsequent generations. The insertion of T-DNA was further analyzed in three independent homozygous lines using inverse PCR, and confirmed the integration of transgene in non-coding region of genomic DNA. Immunoblot results showed that the level of J1-1 proteins, which was not normally accumulated in unripe fruits, accumulated high in transgenic plants but appeared to differ among transgenic lines. Moreover, the expression of jasmonic acid-biosynthetic genes and pathogenesis-related genes were up-regulated in the transgenic lines, which is co-related with the resistance of J1-1 transgenic plants to anthracnose disease. Consequently, the constitutive expression of J1-1 in transgenic pepper plants provided strong resistance to the anthracnose fungus that was associated with highly reduced lesion formation and fungal colonization. These results implied the significance of the antifungal protein, J1-1, as a useful agronomic trait to control fungal disease. © 2014 Seo et al.


Lee G.,Korea Advanced Institute of Science and Technology | Park S.Y.,Korea University | Yum S.,Korea Advanced Institute of Science and Technology | Woo S.,Korea Advanced Institute of Science and Technology | And 6 more authors.
Biochip Journal | Year: 2012

Countless species occur in the marine microalgal domain. Some are used as health functional foods or medical products but many species are harmful such as those that cause the red tide. Therefore, it is necessary to conduct prompt and accurate identification of microalgal species. As it is quite difficult to accurately distinguish all species in terms of morphology, we performed DNA barcoding analysis using molecular markers for more accurate and rapid screening. DNA barcoding analysis, i. e., DNA chip technology, is a powerful method for studies on microalgal taxonomy and biodiversity. We used the mitochondrial cytochrome c oxidase subunit I (mtCOI) as a barcoding gene to identify microalgal species. In this study, the diversity and phylogenetic differences among different microalgae were analyzed. Additionally, a microalgal species-specific probe was screened by 21-23 bp and the result was printed on silylated slide for use in a robotic microarrayer. As a result, we performed a DNA chip assay for each of 25 microalgal species and determined that the COI barcode gene was suitable as a marker gene, as it could identify various microalgae from the Korean South Sea by species. © 2012 The Korean BioChip Society and Springer-Verlag Berlin Heidelberg.


Yang W.S.,Sungkyunkwan University | Jeong D.,Sungkyunkwan University | Yi Y.-S.,Sungkyunkwan University | Park J.G.,Sungkyunkwan University | And 4 more authors.
Mediators of Inflammation | Year: 2013

Caffeic acid (CA) is a phenolic compound that is frequently present in fruits, grains, and dietary supplements. Although CA has been reported to display various biological activities such as anti-inflammatory, anti-cancer, anti-viral, and anti-oxidative effects, the action mechanism of CA is not yet fully elucidated. In this study, the anti-inflammatory action mechanism of CA was examined in lipopolysaccharide (LPS) treated macrophages (RAW264.7 cells) and HCl/EtOH-induced gastritis. CA was found to diminish nitric oxide (NO) and prostaglandin E2 (PGE2) production in LPS-stimulated RAW264.7 cells. Additionally, mRNA levels of tumor necrosis factor (TNF)-α, cyclooxygenase (COX)-2, and inducible NO synthase (iNOS) were downregulated by CA. CA also strongly suppressed the nuclear translocation of AP-1 family proteins and the related upstream signaling cascade composed of interleukin-1 receptor-associated kinase 1 (IRAK1), IRAK4, TGF-β-activated kinase 1 (TAK1), mitogen-activated protein kinase kinase 4/7 (MKK4/7), and c-Jun N-terminal kinase (JNK). In a direct kinase assay, CA was revealed to directly inhibit IRAK1 and IRAK4. CA also ameliorated HCl/EtOH-induced gastric symptoms via the suppression of JNK, IRAK1, and IRAK4. Therefore, our data strongly suggest that CA acts as an anti-inflammatory drug by directly suppressing IRAK1 and IRAK4. © 2013 Woo Seok Yang et al.


Moh S.H.,BIO FD and C Co. | Cho S.H.,Korea University | Kim Y.J.,Chungwoon University | Cho J.C.,Korea University | Lee B.W.,Chungwoon University
Communications in Computer and Information Science | Year: 2012

In this study, sodium hyaluronate was hydrolyzed in order to produce low molecular weight sodium hyaluronate and acetylated low molecular weight sodium. The sodium hyaluronate produced in this study had the average molecular weight that ranged from several hundreds of thousands to 3,500 Da by adjusting the acid concentration and reaction time, and then the sodium hyaluronate was converted to acetylated low molecular weight sodium hyaluronate. The testing on cytotoxicity in HaCaT Human Skin Keratinocytes, all the samples, which were processed with the low molecular weight sodium hyaluronate and acetylated low molecular weight sodium, appeared to have no cytotoxicity. The testing result on anti-wrinkle activity in CCD 986-sk human dermal fibroblasts showed that as the molecular weight low molecular weight sodium hyaluronate reduced, the effects on anti-wrinkle activity was getting better, and increase in the amount of PIP synthesis at the LMHA-5, LMHA-6, which were the acetylated samples, had an excellent effect on anti-wrinkle activity. And the measurement results of anti-inflammation showed that as the molecular weight reduced, the amount of NO generated was getting smaller, and the acetylated samples appeared to have much smaller amount of NO, so that acetylated low molecular weight sodium hyaluronate could be considered to have an excellent anti-inflammation effect. © 2012 Springer-Verlag.


Cho S.H.,Korea University | Moh S.H.,BIO FD and C Co. | Kim Y.J.,Chungwoon University | Cho J.C.,Korea University | Kim Y.S.,Kangwon National University
International Journal of Bio-Science and Bio-Technology | Year: 2014

CM-1,3-β-Glucan was synthesis by introducing carboxy methyl group with molar ratio of monochloroacetic acid, and cytotoxicity, antioxidant, whitening effect, anti-inflammation, and anti-wrinkle characteristics were examined It was confirmed that carboxymethyl was quantitatively introduced to β-glucan. As a result of conducting test on cell stability of human keratinocyte cell lines (HaCaT), it did not present cytoxicity at both 0.1% and 0.5% concentration compared to control group and it was revealed to have no cytotoxicity at all. Presented high cell proliferation rate compared to control group at 0.1% concentration. Although anti-inflammation effect was confirmed with the reduction of COX-2 expression in CMB-1, CMB-5, CMB-6, CMBA-1, and CMBA-3 at 0.1% concentration as a result of conducting COX-2 expression influence test against animal cells, an anti-inflammatory test, it was revealed to have no effect in other substances. As a result of conducting MMP-1 expression influence test, an anti-wrinkle test, great anti-wrinkle effect was confirmed as MMI expression reduced in CMR-1, CMB-2, CMB-3, CMB-4, CMB-6, CMBA-1, and CMBA-3 at 0.1% concentration and CMB-1, CMB-2, CMB-3, CMB-4, and CMBA-1 at 0.5% concentration. © 2014 SERSC.


Nguyen M.T.,University of Ulsan | Koo B.-K.,University of Ulsan | Thi Vu T.T.,University of Ulsan | Song J.-A.,University of Ulsan | And 5 more authors.
PLoS ONE | Year: 2014

Human growth hormone (hGH) is synthesized by somatotroph cells of the anterior pituitary gland and induces cell proliferation and growth. This protein has been approved for the treatment of various conditions, including hGH deficiency, chronic renal failure, and Turner syndrome. Efficient production of hGH in Escherichia coli (E. coli) has proven difficult because the E. coli-expressed hormone tends to aggregate and form inclusion bodies, resulting in poor solubility. In this study, seven N-terminal fusion partners, hexahistidine (His6), thioredoxin (Trx), glutathione S-transferase (GST), maltosebinding protein (MBP), N-utilization substance protein A (NusA), protein disulfide bond isomerase (PDI), and the b′a′ domain of PDI (PDIb′a′), were tested for soluble overexpression of codon-optimized hGH in E. coli. We found that MBP and hPDI tags significantly increased the solubility of the hormone. In addition, lowering the expression temperature to 18°C also dramatically increased the solubility of all the fusion proteins. We purified hGH from MBP-, PDIb′a′-, or Trx-tagged hGH expressed at 18°C in E. coli using simple chromatographic techniques and compared the final purity, yield, and activity of hGH to assess the impact of each partner protein. Purified hGH was highly pure on silver-stained gel and contained very low levels of endotoxin. On average, ∼37 mg, ∼12 mg, and ∼7 mg of hGH were obtained from 500 mL-cell cultures of Trx-hGH, MBP-hGH, and PDIb′a′-hGH, respectively. Subsequently, hGH was analyzed using mass spectroscopy to confirm the presence of two intra-molecular disulfide bonds. The bioactivity of purified hGHs was demonstrated using Nb2-11 cell. © 2014 Nguyen et al.


Lee E.-J.,Chungbuk National University | Moh S.-H.,BIO FD and C Co. | Paek K.-Y.,Chungbuk National University
Bioresource Technology | Year: 2011

This study deals with the effects of initial inoculum density and aeration volume on biomass and bioactive compound production in adventitious roots of Eleutherococcus koreanum Nakai in bulb-type bubble bioreactors (3-L capacity). While the fresh and dry weights of the roots increased with increasing inoculum density, the highest percentage dry weight and accumulation of total target compounds (eleutheroside B and E, chlorogenic acid, total phenolics, and flavonoids) were noted at an inoculum density of 5.0gL-1. Poor aeration volume (0.05vvm) stunted root growth, and high aeration volume (0.4vvm) caused physiological disorders. Moreover, an inoculum density of 5.0gL-1 and an aeration volume of 0.1vvm resulted in the highest concentration of total target compounds and least root death. Such optimization of culture conditions will be beneficial for the large-scale production of E. koreanum biomass and bioactive compounds. © 2011 Elsevier Ltd.


Baque M.A.,Chungbuk National University | Baque M.A.,Sher-e-Bangla Agricultural University | Shiragi M.H.K.,Soil Resource Development Institute SRDI | Moh S.-H.,BIO FDandC Co. | And 2 more authors.
In Vitro Cellular and Developmental Biology - Plant | Year: 2013

To improve root growth and production of bioactive compounds such as anthraquinones (AQ), phenolics, and flavonoids by adventitious root cultures of Morinda citrifolia, the effects of aeration rate, inoculum density, and Murashige and Skoog (MS) medium salt strengths were investigated using a balloon-type bubble bioreactor. The possible mechanisms underlying changes in activities of enzymic (superoxide dismutase, catalase, guaiacol peroxidase, ascorbate peroxidase) and nonenzymic (vitamin E) antioxidants, phenylalanine ammonia lyase, and stress levels (accumulation of hydrogen peroxide and proline, peroxidation of lipids) were also studied. Low aeration rate (0.05 vvm [air volume/culture volume/min]) accelerated accumulation of root fresh weight and dry weight (DW). High aeration rates (0.1 to 0.3 vvm) stimulated accumulation of AQ, phenolics, and flavonoids and reduced root growth. Low inoculum densities (5 and 10 g l-1) increased accumulation of those metabolites but inhibited root growth. Culture of adventitious roots with high concentrations of MS salts (1× and 1.5× MS) resulted in induction of oxidative stress that strongly inhibited root growth. Overall, an aeration rate of 0.05 vvm, 15 g l-1 inoculum density, and half-strength (0.5×) MS medium were optimal for enhancing accumulation of root dry biomass (4.38 g l-1), AQ (103.08 mg g-1 DW), phenolics (54.81 mg g-1 DW), and flavonoids (49.27 mg g-1 DW). © 2013 The Society for In Vitro Biology.


Park D.-H.,Dongshin University | Jung D.H.,BIO FDandC Co. | Kim S.J.,BIO FDandC Co. | Kim S.H.,Nutrex Technology Co. | Park K.M.,Dongshin University
BMC Biochemistry | Year: 2014

Background: The cosmetics market has rapidly increased over the last years. For example, in 2011 it reached 242.8 billion US dollars, which was a 3.9% increase compared to 2010. There have been many recent trials aimed at finding the functional ingredients for new cosmetics. Gallic acid is a phytochemical derived from various herbs, and has anti-fungal, anti-viral, and antioxidant properties. Although phytochemicals are useful as cosmetic ingredients, they have a number of drawbacks, such as thermal stability, residence time in the skin, and permeability through the dermal layer. To overcome these problems, we considered conjugation of gallic acid with a peptide. Results: We synthesized galloyl-RGD, which represents a conjugate of gallic acid and the peptide RGD, purified it by HPLC and characterized by MALDI-TOF with the aim of using it as a new cosmetic ingredient. Thermal stability of galloyl-RGD was tested at alternating temperatures (consecutive 4°C, 20°C, or 40°C for 8 h each) on days 2, 21, 41, and 61. Galloyl-RGD was relatively safe to HaCaT keratinocytes, as their viability after 48 h incubation with 500 ppm galloyl-RGD was 93.53%. In the group treated with 50 ppm galloyl-RGD, 85.0% of free radicals were removed, whereas 1000 ppm galloyl-RGD suppressed not only L-DOPA formation (43.8%) but also L-DOPA oxidation (54.4%). Conclusions: Galloyl-RGD is a promising candidate for a cosmetic ingredient. © 2014 Park et al.; licensee BioMed Central Ltd.

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