Time filter

Source Type

Jeju, South Korea

Jeong S.Y.,Chosun University | Qian Z.-J.,Marine Life Research | Jin Y.-J.,Bio Convergence Center | Kim G.-O.,Bio Convergence Center | And 2 more authors.
Natural Product Sciences | Year: 2012

In the present work, we have collected 19 species of macroalgae (9 Phaeophta and 10 Rhodophyta) from all around of Korea: Dictyopteris divaricata, D. prolifera, Myelophycus cavus, Papenfussiella kuromo, Petalonia zosterifolia, Petrospongium rugosum, Rugulopteryx okamurae, Sargassum fulvellum, S. muticum, Callophyllis japonica, Gloiopeltis tenax, Gracilaria longissima, Gracilaria vermiculophylla, Grateloupia asiatica, Grateloupia lanceolata, Grateloupia sparsa, Grateloupia turuturu, Grateloupia sp, and Polyopes affinis. The macroalgal species were extracted by 70% ethanol (EtOH) for 24 h and evaluated its inhibitory effects on αglucosidase. Among ethanol extracts, Myelophycus cavus showed the most effectively inhibitory activity (IC 50, 2.17μg/ml) against α-glucosidase, followed by Sargassum fulvellum (IC50, 8.13 μg/ml), Dictyopteris prolifera (IC 50, 16.66 μg/ml), Rugulopteryx okamurae (IC50, 50.63 μg/ml), and Petrospongium rugosum (IC50, 101.62 μg/ ml). Furthermore, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay showed no cytotoxicity on mouse pre-adipocytes cell line (3T3-L1). These results suggest that some edible macroalgae merit further evaluation for clinical usefulness as anti-diabetic functional foods.

Kim K.-N.,Korea Basic Science Institute | Ham Y.M.,Jeju Biodiversity Research Institute | Moon J.-Y.,Bio Convergence Center | Kim M.-J.,Bio Convergence Center | And 7 more authors.
Food Chemistry | Year: 2012

The present study was designed to evaluate the molecular mechanisms of the action of acanthoic acid (ACAN) from Acanthopanax koreanum (Araliaceae) against HL-60 human promyelocytic leukaemia cells. ACAN reduced the proliferation of HL-60 cells in a dose- and time-dependent manner accompanied by the induction of apoptosis. Possible mechanisms of ACAN-induced apoptosis were also examined. The results showed that ACAN-induced the phosphorylation of members of the mitogen-activated protein kinase (MAPK) family, c-Jun N-terminal kinase (JNK), p38 MAPK (p38), and extracellular signal-regulated kinase (ERK). A specific p38 MAPK inhibitor (SB203580) significantly blocked ACAN-induced apoptosis and cell viability, whereas an ERK inhibitor (PD98059) and JNK inhibitor (SP600125) had no effect. Moreover, ACAN induced the cleavage of caspase-3 and poly-ADP-ribose polymerase (PARP), and decreased the level of Bcl-xL, but these effects were inhibited by SB203580 pre-treatment. These results strongly suggest that ACAN may have cancer chemopreventive and therapeutic potential, due to its ability to activate the p38 MAPK-mediated signalling pathways. © 2012 Elsevier Ltd. All rights reserved.

Park K.,Korea Institute of Science and Technology | Lee J.S.,Korea Institute of Science and Technology | Choi J.S.,Korea Institute of Science and Technology | Nam Y.-J.,Korea Institute of Science and Technology | And 6 more authors.
Phytotherapy Research | Year: 2016

Asthma is a chronic inflammatory disease of lung airways, and pharmacological inhibitors of cyclic adenosine monophosphate-specific phosphodiesterase 4 (PDE4) have been considered as therapeutics for the treatment of asthma. However, development of PDE4 inhibitors in clinical trials has been hampered because of the severe side effects of non-selective PDE4 inhibitors. Here, screening of a plant extract library in conjunction with dereplication technology led to identification of baicalin as a new type of PDE4-selective inhibitor. We demonstrated that while rolipram inhibited the enzyme activity of a range of PDE4 subtypes in in vitro enzyme assays, baicalin selectively inhibited the enzyme activity of PDE4A and 4B. In addition, baicalin suppressed lipopolysaccharide-induced TNF-α expression in macrophage where PDE4B plays a key role in lipopolysaccharide-induced signaling. Furthermore, baicalin treatment in an animal model of allergic asthma reduced inflammatory cell infiltration and TNF-α levels in bronchoalveolar lavage fluids, indicating that the antiinflammatory effects of baicalin in vivo are attributable, in part, to its ability to inhibit PDE4. © 2015 John Wiley & Sons, Ltd.

Discover hidden collaborations