Such-Sanmartin G.,Bio analysis Research Group |
Bosch J.,Bio analysis Research Group |
Segura J.,Bio analysis Research Group |
Segura J.,University Pompeu Fabra |
And 2 more authors.
Clinical Journal of Sport Medicine | Year: 2011
Objective: In the detection of human growth hormone (GH) abuse, the approach based on altered GH-related biomarkers is also being considered with respect to its application within the context of a biological passport. As a potential biomarker, mannan-binding lectin (MBL), which is reported to respond to recombinant GH (rGH) administration, is evaluated here. Design: Randomized and single blind and approved by the Ethical Committee (Comité Itico de Investigación Clínica-Instituto Municipal de Asistencia Sanitaria). PARTICIPANTS:: One group of 12 male subjects (24.2 ± 2.2 years; 76.1 ± 6.1 kg) was studied. Interventions: Mannan-binding lectin concentration was measured in 12 healthy individuals after subcutaneous daily doses of 6 IU of rGH administration. Mannan-binding lectin serum concentration increased after rGH administration. Mannan-binding lectin concentration increases were observed 48 hours after the first administration and remained elevated for several days after the final dose. Main Outcome Measures: Mannan-binding lectin concentration increase and elapsed time to recover initial MBL values after the last rGH administration. Results: Absolute values displayed high interindividual variability, and 1 individual did not show any MBL increase (potential MBL deficiency). Mannan-binding lectin protein showed a clear concentration increase after continued rGH administration, despite the high heterogeneity found between individuals. Conclusions: The use of MBL as a complementary GH-related biomarker could be of interest, taking advantage of the high increases (up to 700%) and the relatively slow recovery time. © 2011 Lippincott Williams & Wilkins. Source