Binzhou City Center Hospital
Binzhou City Center Hospital
Xu Z.,Shuyang Peoples Hospital |
Yan Y.,1St Peoples Hospital Of Jinan |
He J.,1St Peoples Hospital Of Jinan |
Shan X.,Binzhou City Center Hospital |
Wu W.,Nanjing Medical University
Medical Science Monitor | Year: 2017
Background: The pathological mechanism of Barrett’s esophagus (BE) is still unclear. In the present study, pathway cross-talks were analyzed to identify hub pathways for BE, with the purpose of finding an efficient and cost-effective detection method to discover BE at its early stage and take steps to prevent its progression. Material/Methods: We collected and preprocessed gene expression profile data, original pathway data, and protein-protein interaction (PPI) data. Then, we constructed a background pathway cross-talk network (BPCN) based on the original pathway data and PPI data, and a disease pathway cross-talk network (DPCN) based on the differential pathways between the PPI data and the BE and normal control. Finally, a comprehensive analysis was conducted on these 2 networks to identify hub pathway cross-talks for BE, so as to better understand the pathological mechanism of BE from the pathway level. Results: A total of 12 411 genes, 300 pathways (6919 genes), and 787 896 PPI interactions (16 730 genes) were separately obtained from their own databases. Then, we constructed a BPCN with 300 nodes (42 293 interactions) and a DPCN with 296 nodes (15 073 interactions). We identified 4 hub pathways: AMP signaling pathway, cGMP-PKG signaling pathway, natural killer cell-mediated cytotoxicity, and osteoclast differentiation. We found that these pathways might play important roles during the occurrence and development of BE. Conclusions: We predicted that these pathways (such as AMP signaling pathway and cAMP signaling pathway) could be used as potential biomarkers for early diagnosis and therapy of BE. © Med Sci Monit.
Zhang Y.,The First Hospital of Shijiazhuang City |
Du W.,Henan Province Hospital of TCM |
Chen Z.,Binzhou City Center Hospital |
Xiang C.,The First Hospital of Shijiazhuang City
Experimental Cell Research | Year: 2017
Tumor-associated macrophages (TAMs) polarization represents a key regulatory process of tumor progression. However, the underlying mechanisms are unclear. This study aimed to investigate the relationship between secreted phosphoprotein 1 (SPP1) and TAMs in lung adenocarcinoma cells. THP-1 monocytes were differentiated into macrophages using PMA. PMA-treated THP-1 cells were co-cultured with human A549 cells culture supernatant. SPP1 expression in TAMs isolated from lung adenocarcinoma tissues and PMA-treated THP-1 cells were measured. Macrophage polarization was identified by flow cytometric analysis. Cell migration and apoptosis were assessed by Transwell migration assays and flow cytometric analysis, respectively. SPP1 is highly expressed in tumor tissues and TAMs isolated from patients with an advanced TNM stage, and also in PMA-treated THP-1 cells. Co-culture with A549 cells strongly induced SPP-1 expression as well as M2 polarization of THP-1 cells, but it had little effect on short hairpin SPP1 (shSPP1)-transfected THP-1 cells. Interestingly, programmed death ligand 1 (PD-L1), a critical regulator of M2 polarization, was downregulated in SPP1 knockdown THP-1 cells. Inhibition of PD-L1 induced a greater decline of the M2 markers IL-10 and Arg-1 but an increase in the M1 markers IL-12 and TNF-α. In addition, SPP1 knockdown in THP-1 cells can mitigate migration but promote apoptosis of A549 cells, and PD-L1 inhibition can further enhance this effect. THP-1 cells co-cultured with A549 cells attenuated CD4+ T-cell activation, whereas SPP1 inhibition restored T-cell activation. These results highlight the importance of SPP1 in mediating macrophage polarization and lung cancer evasion, suggesting a potential therapeutic target for lung cancer. © 2017 Elsevier Inc.
Zhao H.,Binzhou City Center Hospital |
Cao Y.,Binzhou City Center Hospital |
Wang G.,Binzhou City Center Hospital |
Luo Z.,Weifang Medical University
Oncology Letters | Year: 2017
We investigated the expression of FOXC2, PinX1, Ki-67 and Cyclin D1 in cutaneous cell carcinoma. We collected 30 cutaneous squamous cell carcinoma (SCC), 30 cutaneous basal cell carcinoma (BCC) and 30 normal skin tissues. The protein expression and gene expression of FOXC2, endogenous telomerase-inhibiting gene PinX1, Ki-67 and Cyclin D1 was measured by immunohistochemistry (IHC) and semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), respectively. In SCC and BCC tissues, the positive rate of protein expression and mRNA level of PinX1 were both significantly lower than those in normal tissues. However, the positive rate of protein expression and mRNA level of FOXC2, Ki-67 and Cyclin D1 were significantly higher than those in normal tissues (p<0.05). There was no significant difference between SCC and BCC (p>0.05). In conclusion, FOXC2 may participate in the carcinogenesis process of SCC and BCC. It may also correlate with the expression PinX, Ki-67 and Cyclin D1. However, FOXC2 alone cannot be used as a diagnostic indicator of SCC. © 2017, Spandidos Publications. All rights reserved.
Lu X.,Binzhou Peoples Hospital |
Wang J.,Binzhou Peoples Hospital |
Shan X.,Binzhou City Center Hospital |
Li Y.,Hanzhong Central Hospital
Journal of B.U.ON. | Year: 2017
Purpose: The purpose in this study was to select key genes related to ovarian cancer. Methods: The gene expression profiles of E-GEOD-6008, E-GEOD-26712, E-GEOD-27651, E-GEOD-14001 were obtained from ArrayExpress database (httpj/www.ebi.ac.UK/ar-rayexpressl). Following data recruitment and preprocessing, differentially expressed genes (DEGs) were characterized using Significance Analysis of Microarrays (SAM). Then, a differential expression network (DEN) was constructed using Cytoscope 2.1 software based on differential and non-differential interactions. Pathway analysis was performed based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database using Pathway Analysis with the nodes contained in the main DEN. Centrality analysis on the DEN was conducted to selected HUB genes. And last, western blot was performed on the selected genes in an independent sample set. Results: A total of 370 samples (347 ovarian tumors and 23 controls) were selected. In all, 490 DEGs were obtained, which contained 59 upregulated and 431 downregulated genes. A DEN including 875 gene pairs (1028 nodes) was constructed There were 7 pathways by analyzing the nodes contained in the main DEN. Five HUB genes were gained, and three (UBC, ELAVL1, SIRT1) were both HUB genes and disease genes. Meanwhile, SIRT1 and NEDD4 were down-regulated genes. Verification experiments indicated that the expression ofSIRTl and ELAVL1 in the disease group and the normal group were significantly changed. Conclusions: This study showed that SIRT1 could be chosen as a potential biomarker for promoting detection of ovarian cancer, so as to further understand the molecular pathogenesis of this disease.
Wang H.,Binzhou City Center Hospital |
Wang H.,Binzhou Peoples Hospitalshandong |
Chang Y.,Binzhou City Center Hospital |
Chang Y.,Binzhou Peoples Hospitalshandong |
Liang H.,Binzhou City Center Hospital
Pakistan Journal of Medical Sciences | Year: 2017
Objective: To analyze the clinical effect of tamsulosin and Solifenacin in the treatment of benign prostatic hyperplasia in combination with overactive bladder and its safety. Another objective was to investigate the clinical effect and safety of mega dose of tamsulosin in the treatment of benign prostatic hyperplasia in combination with overactive bladder. Methods: One hundred and twenty-four patients who were admitted to the Dept. of Urology at Binzhou People’s Hospital, China with confirmed benign prostatic hyperplasia (BPH) with overactive bladder were randomly divided into two groups. Sixty-two patients in the control group were treated with tamsulosin, while sixty-two patients in the observation group were treated with tamsulosin in combination with solifenacin. The treatment of both groups lasted for 12 weeks. The effect and adverse reaction were compared between the two groups. Results: The international prostate symptom score (IPSS), quality of life (QOL), and overactive bladder symptom score (OABSS), Qmax, pulmonary vascular resistance (PVR), daytime urination frequency, urgent urination frequency, urge urinary incontinence frequency and night urinary frequency of both groups improved after treatment, and the difference had statistical significance (P<0.05). The differences of the observation indexes (except PVR) in the observation group before and after treatment was significantly different with those of the control group (P<0.05). The incidence of adverse reactions in the observation group was lower than that in the control group, but the difference had no statistical significance (X2=2.843, P>0.05). Conclusion: Treating benign prostatic hyperplasia in combination with overactive bladder with tamsulosin in combination with solifenacin is more effective than tamsulosin, without significantly increasing adverse reactions. Thus the therapy is worth clinical promotion. © 2017, Professional Medical Publications. All rights reserved.
Mou Z.,Peoples Hospital Of Rizhao |
Xu X.,Binzhou City Center Hospital |
Dong M.,Peoples Hospital Of Zoucheng |
Xu J.,Taian City Central Hospital
Medical Science Monitor | Year: 2016
Background: The purpose of our study was to investigate the role of microRNA (miR)-148b in cervical cancer. Material/Methods: The expression of miR-148b was determined in HPV-16-immortalized cervical epithelial cell line CRL-2614 cells and in cervical cancer cell line HeLa cells. The miR-148b mimics or scrambled RNA were then transfected into Hela cells. Forty-eight hours after transfection, the mRNA expression of miR-148b and DNA methyltransferase 1 (DNMT1) were confirmed. Cell proliferation ability (cell viability and colony formation ability), invasion ability, and apoptosis were assessed after transfection with miR-148b mimics or scrambled RNA, as well as the protein expression of cyclin D1 and caspase-3. Results: The expression of miR-148b was significantly downregulated in HeLa cells compared with CRL2614 cells (P<0.05), but was statistically upregulated by transfection with miR-148b mimics compared with the cells transfected with scrambled RNA (P<0.05). Also, we found that the expression of DNMT1 was significantly decreased by transfection with miR-148b mimics (P<0.05). Additionally, miR-148b mimics significantly decreased the cell proliferation ability and invasion ability, and statistically induced apoptosis. Furthermore, the expression of cyclin D1 protein was significantly decreased and the expression of caspase-3 protein was significantly increased by miR-148b mimics compared with that in the cells transfected with scrambled RNA (P<0.05). Conclusions: Our results suggest that overexpression of miR-148b protects against cervical cancer by inducing G1/S-phase cell cycle arrest and apoptosis through caspase-3-dependent manner, and overexpression of miR-148b might develop a therapeutic intervention for cervical cancer. © Med Sci Monit.
Chen H.,Binzhou City Center Hospital |
Liu X.,Binzhou City Center Hospital |
Xu L.,Jining No 1 Peoples Hospital
Experimental and Therapeutic Medicine | Year: 2016
The aim of the study was to examine the relationship between incisal condition after loop electrosurgical excision procedure (LEEP) operation for cervical intraepithelial neoplasia (CIN) patients and prognosis. A study of high-risk incisal margin positive cases was also performed. We compared the differences in the prognosis of the 1-year follow-up visit in the 120 CIN patients admitted to the hospital during the period from April 2013 to April 2014. A total of 43 cases of positive incisal margin (35.8%), and 77 negative cases were included in the study. The differences in age, course of disease, and CIN level between the two groups showed no statistical significance (P>0.05). In the positive group, the positive ratio was significantly higher than that of the negative group (P<0.05). The positive incisal margin showed a significant positive correlation with human papillomavirus (HPV) positivity (r=0.327, P=0.035). The condition of most patients with positive incisal margin and HPV was critical, which was followed by positive incisal margin and negative HPV. The patients with the best prognosis were those with significant negative incisal margin as well as HPV (P<0.05). In conclusion, the positive incisal margin after LEEP operation was associated with relapse of the disease. Thus, considering the HPV test into consideration is valuable for the prognosis of the disease. © 2016, Spandidos Publications. All rights reserved.
Sun Y.,Fujian Medical University |
Huang Z.-Y.,Fujian Medical University |
Sun Q.-R.,Binzhou City Center Hospital |
Qiu L.-P.,Binzhou City Center Hospital |
And 2 more authors.
Acta Cardiologica | Year: 2016
Objective Studies suggest that continuous positive airway pressure (CPAP) therapy may decrease blood pressure for patients with obstructive sleep apnoea (OSA). However, these benefits are not completely clear. Method We undertook a meta-analysis of randomized trials identified in systematic researches of MEDLINE, EMBASE, and the Cochrane Database. Results Twelve studies (1,720 patients) were included. Overall, CPAP therapy significantly decreased 24-h systolic blood pressure (SBP) (MD, -2.03; 95% CI, -3.64 to -0.42; P = 0.01; I2 = 0%) and 24-h diastolic blood pressure (DBP) (MD, -1.79; 95% CI, -2.89 to -0.68; P = 0.001; I2 = 0%) compared with the control group. Meanwhile, CPAP was associated with significantly lower daytime DBP (MD, -1.43; 95% CI, -2.67 to -0.19; P = 0.02; I2 = 0%), nighttime SBP (MD, -3.94; 95% CI, -5.85 to -2.04; P < 0.0001; I2 = 34%), nighttime DBP (MD, -1.64; 95% CI, -2.88 to -0.40; P = 0.009; I2 = 0%), clinic SBP (MD, -4.53; 95% CI, -6.07 to -2.99; P < 0.00001; I2 = 70%) and clinic DBP (MD, -2.94; 95% CI, -4.16 to -1.72; P < 0.00001; I2 = 56%). Furthermore, the risk of cardiovascular events was significantly decreased in the CPAP group (OR, 0.59; 95% CI, 0.36 to 0.98; P = 0.04; I2 = 0%). Conclusions CPAP therapy was associated with a significantly decreased level of BP and cardiovascular events. However, larger randomized studies are needed to confirm these findings. © 2016, Acta Cardiologica. All rights reserved.
PubMed | Liaocheng Peoples Hospital, Weifang Peoples Hospital and Binzhou City Center Hospital
Type: | Journal: Medical science monitor : international medical journal of experimental and clinical research | Year: 2016
BACKGROUND Pediatric sepsis has high morbidity in children, may lead to acute kidney injury (AKI), and further aggravate the disease. Baicalin is a kind of flavonoid in Scutellaria baicalensis Georgi and has been reported to protect against several diseases, but its roles in septic AKI remain unclear. This study aimed to uncover the effects of baicalin in AKI during pediatric sepsis. MATERIAL AND METHODS Blood urea nitrogen (BUN) and serum creatinine (Cr) levels were detected in 50 pediatric patients, who underwent basic therapy with or without baicalin adjunctive therapy. Mouse sepsis models were constructed by cecal ligation and puncture (CLP) and treated with baicalin intragastrically, after which BUN and Cr examination, TUNEL apoptosis assay, and expression analyses of BAX and BCL2 were performed. RESULTS Baicalin adjunctive therapy significantly decreased BUN and Cr levels in pediatric sepsis patients (P<0.05). CLP led to elevated BUN and Cr levels in the mouse model (P<0.01), indicating kidney injury accompanied by sepsis. Baicalin decreased BUN and Cr levels (P<0.05), and reduced the apoptotic cell percent in the renal tissue (P<0.05) of the CLP model. It inhibited BAX and promoted BCL2 in the renal tissue, which was consistent with cell apoptosis changes. CONCLUSIONS Baicalin is capable of suppressing renal cell apoptosis and protecting against AKI in pediatric sepsis. This study provides a potential adjunctive therapy for treating AKI in pediatric sepsis, and further research is necessary to reveal its deeper mechanisms.
PubMed | Tsinghua University, Fujian Medical University, Binzhou City Center Hospital and Shenzhen Baoan District Songgang Peoples Hospital
Type: Journal Article | Journal: Sleep & breathing = Schlaf & Atmung | Year: 2016
Continuous positive airway pressure (CPAP) therapy may decrease the risk of mortality and cardiovascular events in patients with obstructive sleep apnea. However, these benefits are not completely clear.We undertook a meta-analysis of randomized clinical trials identified in systematic searches of MEDLINE, EMBASE, and the Cochrane Database.Eighteen studies (4146 patients) were included. Overall, CPAP therapy did not significantly decrease the risk of cardiovascular events compared with the control group (odds ratio (OR), 0.84; 95% confidence intervals (CI), 0.62-1.13; p=0.25; I (2)=0%). CPAP was associated with a nonsignificant trend of lower rate of death and stroke (for death: OR, 0.85; 95% CI, 0.35-2.06; p=0.72; I (2)=0.0%; for stroke: OR, 0.56; 95% CI, 0.18-1.73; p=0.32; I (2)=12.0%), a significantly lower Epworth sleepiness score (ESS) (mean difference (MD), -1.78; 95% CI, -2.31 to -1.24; p<0.00001; I (2)=76%), and a significantly lower 24h systolic and diastolic blood pressure (BP) (for 24h systolic BP: MD, -2.03mmHg; 95% CI, -3.64 to -0.42; p=0.01; I (2)=0%; for diastolic BP: MD, -1.79mmHg; 95% CI, -2.89 to -0.68; p=0.001; I (2)=0%). Daytime systolic BP and body mass index were comparable between the CPAP and control groups. Subgroup analysis did not show any significant difference between short- and mediate-to-long-term follow-up groups with regard to cardiovascular events, death, and stroke.CPAP therapy was associated with a trend of decreased risk of cardiovascular events. Furthermore, ESS and BP were significantly lower in the CPAP group. Larger randomized studies are needed to confirm these findings.