Fu S.,Binzhou Animal Husbandry and Veterinary Research Academy |
Fu S.,Shandong Lvdu Ante Veterinary Drug Industry Co. |
Qu G.,Binzhou Animal Husbandry and Veterinary Research Academy |
Qu G.,Shandong Lvdu Biological Technology Co. |
And 10 more authors.
Applied Biochemistry and Biotechnology | Year: 2011
During the last 10 years, with the development of loop-mediated isothermal amplification (LAMP) method, it has been widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency, and outstanding specificity. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA. Expensive equipment are not necessary to acquire a high level of precision, and there are fewer preparation steps compared to conventional PCR and real-time PCR assays. This paper briefly summarized the applications of LAMP method in pathogenic microorganisms, genetically modified ingredients, tumor detection, and embryo sex identification. © 2010 Springer Science+Business Media, LLC.