Cui L.-Q.,Binhai New Area Dagang Hospital of Tianjin |
Wang X.-R.,Tianjin Medical University |
Jiang Z.-M.,Tianjin Fifth Central Hospital |
Zhang M.,Binhai New Area Dagang Hospital of Tianjin |
And 5 more authors.
Tumor | Year: 2013
Objective: To investigate the functional role of CXCL12/CXCR4 (chemokine receptor 4) in bone metastatic breast cancer cells. Methods: After the breast cancer MDA-MB-231 cells were cultured in medium containing different concentrations of CXCL12, the expression of CXCR4 in MDA-MB-231 cells was detected by immunocytochemistry and the migration ability of MDA-MB-231 cells was detected by Transwell double chamber culture system. The MDA-MB-231 cells or MDA-MB-231 cells plus 100 nmol/L CXCL12 were injected into left ventricle of nude mice to establish the model of bone metastasis from breast cancer cells. The condition of bone metastasis was analyzed by pathological examination, and then the expression level of CXCL12 in metastatic bone lesions was detected by Western blotting. Results: The expression level of CXCR4 in MDA-MB-231 cells was significantly associated with CXCL12 in dose-related manner (r = 0.92, P < 0.05). The percentage of migratory MDA-MB-231 cells was gradually increased with the increasing concentration of CXCL12. As compared with MDA-MB-231 xenograft model in nude mice, the numbers of metastatic lesions in the vertebrae and sternum were much more in MDA-MB-231 xenograft model in nude mice treated with CXCL12 (P < 0.01), meanwhile the expression levels of CXCL12 protein were also higher in metastatic lesions in vertebrae and sternum. Conclusion: The CXCL12/ CXCR4 role-axis is closely related to bone metastasis from breast cancer cells, which may become a target of gene therapy. Copyright © 2013 by TUMOR.