Bijie Tobacco Company of Guizhou Province

Bijie, China

Bijie Tobacco Company of Guizhou Province

Bijie, China
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Liu J.,Zhejiang Agriculture And forestry University | Yang X.,Zhejiang Agriculture And forestry University | Lu K.,Zhejiang Agriculture And forestry University | Zhang X.,Zhejiang Agriculture And forestry University | And 2 more authors.
Huanjing Kexue Xuebao/Acta Scientiae Circumstantiae | Year: 2015

An incubation experiment was conducted to investigate the effect of biochars derived from bamboo (BB) and rice straw (RSB) on the bioavailability, transformation and redistribution of heavy metals in a sandy loam paddy soil contaminated with Cd, Cu, Pb and Zn. Biochars with two mesh sizes (< 0.25 mm and <1 mm) were applied at three application rates (0, 1% and 5%, w/w) to the soil. After one-year incubation with a constant temperature at (25 ±1)℃, pH value and concentrations of available phosphorus and DTPA (Diethylene Triamine Pentacetate Acid)-extractable heavy metals in soil samples was analyzed. In addition, BCR (European Community Bureau of Reference) sequential extraction procedure was conducted to analyze the chemical forms of heavy metals. The results indicated that the addition of rice straw biochar to soil significantly (p<0.05) increased soil pH, and the soil pH value of the RSB-5% treatment was the highest. The available phosphorus concentration in soil increased with the application rates of rice straw biochar. However, the addition of bamboo biochar had no significant effect on soil pH and available phosphorus concentration. The RSB-5% treatment showed the greatest decrease in concentrations of DTPA-extractable Cd, Cu, Pb and Zn in soil, with remarkable decrease by 34.5%, 50.1%, 52.5% and 52.1%, respectively. In the fine (0.25 mm) rice straw biochar treatments, the soil acid-soluble fractions of Cd and Cu were transformed to reducible and oxidizable fractions, whereas the acid-soluble Zn was mainly transformed to reducible Zn. Adding the coarse (1 mm) bamboo biochar to soil enhanced transformation of Cu and Zn from acid soluble fraction to reducible, oxidizable and residual fractions. Compared with the coarse rice straw biochar, the fine rice straw biochar was more effective in transforming acid-soluble Pb to oxidizable and reducible fractions. Both bamboo biochar and rice straw biochar treatments can alter the transformation and availability of heavy metals in the soil, and the effect was more significant in the 5% biochar treatments. © 2015, Science Press. All right reserved.


Zhang S.J.,Sichuan Agricultural University | Zhang S.J.,Agricultural Quality Inspection and Testing Center | Li T.X.,Sichuan Agricultural University | Huang H.G.,Sichuan Agricultural University | And 8 more authors.
International Journal of Environmental Science and Technology | Year: 2014

Athyrium wardii (Hook.) is a promising herbaceous plant species for phytostabilization of cadmium (Cd)-contaminated sites with large biomass and fast growth rate. However, little information is available on its tolerance mechanisms toward Cd. To further understand the mechanisms involved in Cd migration, accumulation and detoxification, the present study investigated subcellular distribution and chemical forms of Cd in the mining ecotypes and corresponding non-mining ecotypes of A. wardii via greenhouse pot experiment. Subcellular fractionation of Cd-containing tissues demonstrated that the majority of the element was mainly located in soluble fraction in cell walls. This indicated that both the vacuoles and cell walls might be evolved the Cd tolerance mechanisms to protect metabolically active cellular compartments from toxic Cd concentrations. Meanwhile, Cd taken up by the plant existed in different chemical forms. Results showed that the majority of Cd in plant was in undissolved Cd-phosphate complexes (extracted by 2 % CH3COOH), followed by water-soluble Cd-organic acid complexes, Cd(H2PO4)2, pectates and protein form (extracted by deionized water and 1 M NaCl), whereas only small amount of Cd in roots was in inorganic form (extracted by 80 % ethanol), which suggests low capacity to be transported to aboveground tissues. It could be suggested that Cd integrated with undissolved Cd-phosphate complexes in cell wall or compartmentalization in vacuole might be responsible for the adaptation of the mining ecotypes of A. wardii to Cd stress. © 2013 Islamic Azad University (IAU).


Huang H.,Zhejiang University | Huang H.,Bijie Tobacco Company of Guizhou Province | Wang K.,Zhejiang University | Zhu Z.,Zhejiang University | And 5 more authors.
Environmental Science and Pollution Research | Year: 2013

While phytoextraction tools are increasingly applied to remediation of contaminated soils, strategies are needed to optimize plant uptake by improving soil conditions. Mineral nutrition affects plant growth and metal absorption and subsequently the accumulation of heavy metal through hyper-accumulator plants. Microcosm experiments were conducted in greenhouse to examine the effect of different phosphorus (P) sources on zinc (Zn) phytoextraction by Sedum alfredii in aged Zn-contaminated paddy soil. The Zn accumulation, soil pH, microbial biomass and enzyme activity, available Zn changes. and Zn phytoremediation efficiency in soil after plant harvest were determined. Upon addition of P, Zn uptake of S. alfredii significantly increased. Mehlich-3 extractable or the fractions of exchangeable and carbonate-bound soil Zn were significantly increased at higher P applications. Soil pH significantly decreased with increasing P application rates. Soil microbial biomass in the P-treated soils was significantly higher (P < 0. 05) than those in the control. Shoot Zn concentration was positively correlated with Mehlich-3 extractable P (P < 0. 0001) or exchangeable/carbonate-bound Zn (P < 0. 001), but negatively related to soil pH (P < 0. 0001). These results indicate that application of P fertilizers has the potential to enhance Zn mobility and uptake by hyperaccumulating plant S. alfredii, thus increasing phytoremediation efficiency of Zn-contaminated soils. © 2012 Springer-Verlag.


Wu C.,Guizhou Academy of Tobacco Science | Li Y.,Qiannan Tobacco Company of Guizhou Province | Shi H.,Qiannan Tobacco Company of Guizhou Province | Feng Y.,Zunyi Tobacco Company of Guizhou Province | And 3 more authors.
Tobacco Science and Technology | Year: 2014

To accurately evaluate the comprehensive trait of new flue-cured tobacco varieties (lines), the data of 12 trait indexes (including natural leaf number, yield, percentage of high grade leaf, grade index, nicotine content, reducing sugar content, potassium content, bacterial wilt disease index, climate spot disease index, appearance quality score, stem content and total score of sensory evaluation) obtained in 2012 Guizhou regional test were analyzed with fuzzy comprehensive evaluation method. GGE biplot analysis was used to analyze comprehensive evaluation results, conduct varieties (lines) evaluation and testing points evaluation, and analyze the adaptable areas of each variety (line). The results showed that Guiyan 2, 2010A4 and MT-2 obtained better and stabler comprehensive evaluation value; YQ-1 obtained better comprehensive evaluation value in District 1 (Longgang and Weining), as did Guiyan 2 in District 2 (Zunyi, Guiding and Kaili) and 6517 in District 3 (Yinjiang). According to evaluation of testing points, those in Guiding and Zunyi were good in representativeness and discrimination; those in Yinjiang, Weining and Longgang were good in discrimination, but not as good in representativeness; those in Kaili were good in representativeness, but not as good in discrimanation.


Gupta D.K.,Federal University of Santa Maria | Huang H.G.,Bijie Tobacco Company of Guizhou Province | Nicoloso F.T.,Federal University of Santa Maria | Schetinger M.R.,Federal University of Santa Maria | And 5 more authors.
Ecotoxicology | Year: 2013

Plantlets of Pfaffia glomerata (Spreng.) were exposed for 28 days to three different metal/metalloid (Hg, Pb and As) with different levels (Hg 1; As 25, 50, 100 and Pb 100 and 400 μM) to analyze the possible phytochelatin initiation and affects on growth and photosynthetic pigments vis-à-vis metal accumulation potential of plants. The plantlets showed significant Hg, As and Pb accumulation in roots (150, 1267.67 and 2129 μg g-1 DW respectively); however, a low root to shoot metal translocation was observed. It was interesting to note that all tested macronutrient (Mg, K, Ca) was higher in shoots and just opposite in case of micronutrients (Cu, Fe, Zn), was recorded highest in roots. The growth of plantlets (analyzed in terms of length and dry weight) was negatively affected by various metal treatments. In addition, the level of photosynthetic pigments alters significantly in response to all metal/metalloid treatment. In response to all tested metal/metalloids in plants only As induced phytochelatins (PC2, PC3 and PC4) in roots, and in shoots, GSH was observed in all tested metal/metalloids. In conclusion, P. glomerata plantlets could not cooperatively induce phytochelatins under any of Hg and Pb levels. © 2013 Springer Science+Business Media New York.


Wang K.,Zhejiang University | Wang K.,Ningbo University | Chen X.-X.,Ningbo University | Zhu Z.-Q.,Zhejiang University | And 3 more authors.
Environmental Science and Pollution Research | Year: 2014

A microcosm experiment was conducted to investigate the dissipation of available benzo[a]pyrene (BaP) in soils co-contaminated with cadmium (Cd) and pyrene (PYR) during aging process. The available residue of BaP in soil was separated into desorbing and non-desorbing fractions. The desorbing fraction contributed more to the dissipation of available BaP than the non-desorbing fraction did. The concentration of bound-residue fraction of BaP was quite low across all treatments. Within the duration of this study (250 days), transformation of BaP from available fractions to bound-residue fraction was not observed. Microbial degradation was the dominant mechanism of the dissipation of available BaP in the soil. The dissipation of available BaP was significantly inhibited with the increment in Cd level in the soil. The addition of PYR (250 mg kg-1) remarkably promoted the dissipation of available BaP without reducing Cd availability in the soil. The calculated half-life of available BaP in the soil prolonged with the increment in Cd level; however, the addition of PYR shortened the half-life of available BaP by 13.1, 12.7, and 32.8 % in 0.44, 2.56, and 22 mg Cd kg-1 soils, respectively. These results demonstrated that the inhibiting effect of Cd and the promoting effect of PYR on the dissipation of available BaP were competitive. Therefore, this study shows that the bioremediation process of BaP can be more complicated in co-contaminated soils. © 2013 Springer-Verlag Berlin Heidelberg.


Gupta D.K.,CSIC - Experimental Station of El Zaidín | Huang H.G.,Zhejiang University | Huang H.G.,Bijie Tobacco Company of Guizhou Province | Corpas F.J.,CSIC - Experimental Station of El Zaidín
Environmental Science and Pollution Research | Year: 2013

Lead (Pb) is naturally occurring element whose distribution in the environment occurs because of its extensive use in paints, petrol, explosives, sludge, and industrial wastes. In plants, Pb uptake and translocation occurs, causing toxic effects resulting in decrease of biomass production. Commonly plants may prevent the toxic effect of heavy metals by induction of various celular mechanisms such as adsorption to the cell wall, compartmentation in vacuoles, enhancement of the active efflux, or induction of higher levels of metal chelates like a protein complex (metallothioneins and phytochelatins), organic (citrates), and inorganic (sulphides) complexes. Phyotochelains (PC) are synthesized from glutathione (GSH) and such synthesis is due to transpeptidation of γ-glutamyl cysteinyl dipeptides from GSH by the action of a constitutively present enzyme, PC synthase. Phytochelatin binds to Pb ions leading to sequestration of Pb ions in plants and thus serves as an important component of the detoxification mechanism in plants. At cellular level, Pb induces accumulation of reactive oxygen species (ROS), as a result of imbalanced ROS production and ROS scavenging processes by imposing oxidative stress. ROS include superoxide radical (O2 .-), hydrogen peroxide (H2O2) and hydroxyl radical (·OH), which are necessary for the correct functioning of plants; however, in excess they caused damage to biomolecules, such as membrane lipids, proteins, and nucleic acids among others. To limit the detrimental impact of Pb, efficient strategies like phytoremediation are required. In this review, it will discuss recent advancement and potential application of plants for lead removal from the environment. © 2013 Springer-Verlag Berlin Heidelberg.


Feng M.,CAS Zhengzhou Research Institute | Zhang H.,CAS Shanghai Institute of Organic Chemistry | Cao B.,Bijie Tobacco Company of Guizhou Province | Liu S.,CAS Zhengzhou Research Institute | And 2 more authors.
Drug Metabolism and Pharmacokinetics | Year: 2015

8-Methoxypsoralen (8-MOP) is a well established drug in the treatment of various skin diseases. Pretreatment of mice with 8-MOP before administration of 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) significantly reduced the incidence of NNK-induced tumor. The present study was designed to evaluate the in vivo effects of 8-MOP on the bioactivation of NNK in mice. Decrease in the α-hydroxylation of NNK in mouse blood and tissues was observed as the most pronounced effect of 8-MOP. The catalytic property of cytochrome P450 2A5 (CYP2A5) enzyme in mice was determined by the coumarin 7-hydroxylation reaction, suggesting that 8-MOP produced remarkable inhibition on CYP2A5 in female C57BL/6 mice. These results implied that 8-MOP could prevent NNK-induced mutagenesis and tumorigenesis in mice through the inhibition of NNK α-hydroxylation, which may be achieved through the effect of 8-MOP on the bioactivities of CYP2A5. © 2015, The Japanese Society for the Study of Xenobiotics. Published by Elsevier Ltd. All rights reserved.


Han X.,Zhejiang University | Liang C.-F.,Zhejiang University | Li T.-Q.,Zhejiang University | Wang K.,Ningbo University | And 2 more authors.
Journal of Zhejiang University: Science B | Year: 2013

The simultaneous sorption behavior and characteristics of cadmium (Cd) and sulfamethoxazole (SMX) on rice straw biochar were investigated. Isotherms of Cd and SMX were well modeled by the Langmuir equation (R 2>0.95). The calculated maximum adsorption parameter (Q) of Cd was similar in single and binary systems (34 129.69 and 35 919.54 mg/kg, respectively). However, the Q of SMX in a binary system (9 182.74 mg/kg) was much higher than that in a single system (1 827.82 mg/kg). The presence of Cd significantly promoted the sorption of SMX on rice straw biochar. When the pH ranged from 3 to 7.5, the sorption of Cd had the characteristics of a parabola pattern with maximum adsorption at pH 5, while the adsorption quantity of SMX decreased with increasing pH, with maximum adsorption at pH 3. The amount of SMX adsorbed on biochar was positively correlated with the surface area of the biochar, and the maximum adsorption occurred with d 250 biochar (biochar with a diameter of 150-250 μm). Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) showed that the removal of Cd and SMX by rice straw biochar may be attributed to precipitation and the formation of surface complexes between Cd or SMX and carboxyl or hydroxyl groups. The results of this study indicate that rice straw biochar has the potential for simultaneous removal of Cd and SMX from co-contaminated water. © 2013 Zhejiang University and Springer-Verlag Berlin Heidelberg.


Huang H.,Bijie Tobacco Company of Guizhou Province | Xiao M.,CAS Institute of Subtropical Agriculture | Xiao M.,Guangxi Normal University | Liang S.,Guangxi Normal University | And 6 more authors.
Tobacco Science and Technology | Year: 2015

To investigate the changes of soil microbial characteristics in Karst mountain areas caused by the transformation from conventional cultivation into organic cultivation, an experiment was carried out in tobacco fields on which organic cultivation had been conducted for three consecutive years in Jinsha County, Guizhou Province by taking conventional cultivation as the control. The basic soil properties, bacterial and fungal community structure and key influencing factors were studied. The results showed that: comparing with conventional cultivation, the contents of soil organic matter and microbial biomass carbon significantly increased, the contents of soil nutrients (total nitrogen, total potassium, available phosphorus, available potassium, etc) significantly decreased (p<0.05), and the amount of beneficial microorganism groups increased as a result of organic cultivation. The richness and diversity of bacteria were higher than those of fungi were common to both cultural practices. The diversities and microbial communities at phylum level were not obviously different between conventional and organic cultural practices. Bacterial communities dominated by Proteobacteria, Actinobacteria and Acidobacteria(accounted for 54.2%-62.0%), and fungal communities dominated by Ascomycota (accounted for 74.4%). The microbial communities in Karst mountain areas were mainly influenced by the organic matter, nutrient and bulk density in soil. © 2015, Editorial Office of Tobacco Science & Technology. All right reserved.

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