Jian L.,Southwest University |
Jian L.,BiJie College |
Zhang Y.-Z.,BiJie College |
Yu D.-F.,BiJie College |
Zhu L.-Q.,Southwest University
African Journal of Biotechnology | Year: 2010
Fifty-four Cymbidium kanran cultivars from China, Japan and Korea were examined and analyzed by using the successive screening of 3'-end extended random primer amplified polymorphic DNA (ERAPD) markers to determine their molecular diversity and relationships. In ERAPD analyses, the strandspecific DNA sequence of direct oligonucleotide extension sequencing primers was independently read from each of the RAPD fragments without recourse to cloning or strand separation. Eight primers, identified from 80 original RAPD primers, produced strong repetitive polymorphic bands that were used in 3'-end-extended random primer amplified DNA marker analysis. The products of primers ACTGAACGCCCG + ACTGAACGCCGG and ACTGAACGCCC + ACTGAACGCC, linked to the same locus (2.5 - kb), were developed from the original ACTGAACGC RAPD primer; the products of this marker were more stable and specific than the original RAPD marker. Unweighted pair-group mean analysis (UPGMA) grouped them into two clusters based upon geographical traits. We demonstrated that the ERAPD technique is a powerful tool for cultivar identification and establishment of genetic relationships of cultivars in Cymbidium kanran. © 2010 Academic Journals.
Xue X.,Chinese Academy of Sciences |
Xue X.,University of Chinese Academy of Sciences |
Xue X.,Bijie College |
Hao M.,Chinese Academy of Sciences |
Hao M.,Northwest University, China
Acta Agriculturae Scandinavica Section B: Soil and Plant Science | Year: 2011
Nitrate leaching is an important factor affecting N fertilizer consumption in the agroecosystem of the Loess Plateau of China. Therefore, the movement and residual amounts of nitrate within the soil profile under different fertilizer application rates were studied to determine the most appropriate rates of fertilizer application. Soil samples were collected from a longterm experimental site to determine the concentration of nitrate in mid-September 1999 and 2007. The results showed that NO 3 --N had moved more than 100 cm down the soil profile from 1999 to 2007, and two peaks of NO 3 --N were present at different depths after 23 years of high rates of N application. NO 3 --N had leached to depths exceeding 300 cm in plots where-90 kg ha -1 N had been applied alone. At the fertilization rate of 180 kg N ha -1, up to 1500 kg ha -1 residual NO 3 --N was detected, equivalent to 34.8% of the total input of N fertilizer during the experiment. The total amount of residual nitrate increased with increases in the N application rate, but decreased with increases in P 2O 5 application when the N application was up to 90 kg ha -1 or more. The results indicate that fertilization using a 1:1 mixture of N:P 2O 5 at 90 kg ha -1 p.a. could prevent NO 3 --N from leaching in soil used to grow continuous winter wheat (Triticum aestivum L.) crops in the rain-fed agricultural areas of China, while providing optimum yields. © 2011 Taylor & Francis.
Dong S.-W.,Chinese Academy of Agricultural Sciences |
Gao Z.-H.,Chinese Academy of Agricultural Sciences |
Gao Z.-H.,Gansu Agricultural University |
Shen X.-Y.,Bijie College |
And 2 more authors.
Journal of Integrative Agriculture | Year: 2014
Copper nanoparticle is a new material widely used in biological medicine, animal husbandry and industrial areas, but its potential toxicity to human health and environment remains unclear. In order to study the hepatotoxic mechanisms of nanoparticles copper, two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI/TOF MS) of proteomics technology were used to isolate and identify the differentially expressed proteins from liver, which associated with hepatotoxicity induced by copper nanoparticle in rats. In this study, we have screened 15 kinds of proteins related with hepatotoxicity, of which spot8212 was identified as Malate dehydrogenase (Mdh1). The mRNA expression trend of Mdh1 was consistent with the result of 2-DE by RT-PCR validation. Bioinformatics analysis showed that Mdh1 was stable and no signal peptides, subcellular location was in endoplasmic reticulum; it contained many functional sites such as malate dehydrogenase activity signal sites 155LTRLDHNRAKSQI167; α helixes and random coils were the two main elements. Homologous analysis demonstrated high homologous of Mdh1 in rats with mouse and human, and the phylogenetic tree of Mdh1 was constructed. The result indicated that copper nanoparticle could regulate up the Mdh1 protein expression so as to compensate the energy deficit. Energy metabolic disturbance may be a pathway for copper nanoparticle particles to exert the hepatotoxic effects in rats. © 2014 Chinese Academy of Agricultural Sciences.
Shi K.,Southwest University |
Diao C.,Southwest University |
Sun X.,Southwest University |
Zuo T.,Southwest University |
And 3 more authors.
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2013
In view of the evaluation of eco-security of cultivated land requisition-compensation balance system's uncertainty, an improved set pair analysis (SPA) model is quoted that utilizing SPA theory and the malleability of the original relation degree to extend the comprehensive evaluation ranking of the evaluation of eco-security of cultivated land requisition-compensation balance from 3 levels to 4 levels. It can be used for obtaining the evaluation of eco-security of cultivated land requisition-compensation balance level in different years of Yongchuan district based on 18 influential factors with the theory of entropy weight and improved SPA., The comprehensive evaluation results of proposed model of the evaluation of eco-security of cultivated land requisition-compensation balance is compared with the results of improved fuzzy comprehensive evaluation and comprehensive assessment to prove the rationality of improved SPA. The results showed that the eco-security of cultivated land requisition-compensation balance of 2005 were II, the grade of 2009 were III, and the grade of 2015 and 2020 were IV. The whole level of cultivated land requisition-compensation balance in Yongchuan district had been in improving trends that jumping to IV from II from 2005, 2009, 2015 and 2020. Compared with improved fuzzy comprehensive evaluation and comprehensive assessment, the results of three methods are basically consistent. Then it shows the evaluation results of improved SPA are reliable,reasonable and visualized,and the improved SPA is a scientific and practical evaluation method that can more meticulously describe the cultivated land requisition-compensation balance level level.