Bijie Branch of Guizhou Provincial Tobacco Company

Bijie, China

Bijie Branch of Guizhou Provincial Tobacco Company

Bijie, China

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Huang H.,Henan Agricultural University | Huang H.,Bijie Branch of Guizhou Provincial Tobacco Company | Shen Y.,Bijie Branch of Guizhou Provincial Tobacco Company | Yu Q.,Bijie Branch of Guizhou Provincial Tobacco Company | And 3 more authors.
Tobacco Science and Technology | Year: 2016

To investigate the effects of different tobacco varieties and climate on the expression of genes in pigment, nitrogen, starch and sugar metabolism pathway of flue-cured tobacco at mature stage, RT-PCR was used to analyze the gene expression in tobacco leaves of cv. Bina 1 and Jiucaiping 2 planted in Bijie(Guizhou) and Baofeng (Henan). The results showed that the expression levels of zeta-carotene desaturase (ZDS), chlorophyllase (CLH), glutamine synthetase (Gln1-3), nitrite reductase 3 (Nir-3), hexokinase (Hex), sucrose-6-phosphate phosphatase(SPP2)and trehalose-phosphate phosphatase(TPP)in Jiucaiping 2 planted in Bijie were higher than those in Bina 1 planted in Bijie. The results suggested that there were differences in metabolism pathway between Bina 1 and Jiucaiping 2. Nir-3 and cellulose synthase-like protein (CslE) gene expression in Bina 1 planted in Bijie expressed at a lower level and a higher level than those planted in Baofeng, respectively. The expression levels of Hex in Bina 1 and Jiucaiping 2 planted in Baofeng were higher than those planted in Bijie, and the expression of TPP in Jiucaiping 2 was lower than that planted in Bijie. This study indicated that these four genes(Nir-3, CslE, Hex and TPP)were affected mostly by climatic conditions. © 2016, Editorial Office of Tobacco Science & Technology. All right reserved.


Liu B.-Q.,Henan Agricultural University | Xu J.-Y.,Henan Agricultural University | Xu J.-Y.,Huazhong Agricultural University | Huang H.-G.,Bijie Branch of Guizhou Provincial Tobacco Company | And 4 more authors.
Zhiwu Shengli Xuebao/Plant Physiology Journal | Year: 2015

In order to study the molecular mechanism of characteristics of flue-cured tobacco at different altitudes in Wumeng tobacco-growing areas of Guizhou province, the real time quantitative PCR was used to detect gene expression of the related enzymes in carbon and nitrogen metabolism of flue-cured tobacco. The results indicated that during vigorous growth stage vacuolar invertase (VIN) gene of flue-cured tobaccos in middle and high altitude areas was expressed more actively, and during maturing period VIN gene in low altitude areas was expressed slightly strongly. And Sucrose synthase (SS) and sucrose phosphate synthase (SPS) genes in high altitude areas were expressed more strongly during vigorous growth stage, but were expressed relatively strongly in low altitude areas and more weakly in middle altitude areas during maturing period. During the four sampling periods the higher the altitudes, the more strongly sucrose-6-phosphate phosphatase (SPP) gene was expressed, granule-bound starch synthase I (GBSSI) gene in high altitude areas was expressed most strongly. SBE gene in high altitude areas was expressed more strongly during vigorous growth stage, but relatively strongly in low altitude areas after transplanting 80 days. Based on the analysis of the different altitudes during the same period, with the altitudes lowering gradually, nitrate reductase (NR) gene was expressed more strongly. During the four sampling periods glutamine synthase (GS1-3 and GS1-5) genes in low altitude areas were expressed more strongly than those in middle and high altitude areas. ©, 2015, Science Press. All right reserved.


Yu Q.,Henan Agricultural University | Yu Q.,Bijie Branch of Guizhou Provincial Tobacco Company | Fu Y.,Henan Agricultural University | Li W.,Shanghai Tobacco Group Co. | And 4 more authors.
Tobacco Science and Technology | Year: 2015

To reveal the distribution characteristics of reducing sugar/nicotine ratio of flue-cured tobacco in Bijie tobacco growing areas and relationship thereof with sensory quality, the reducing sugar/nicotine ratio of 342 samples of C3F from Bijie was analyzed by descriptive statistical analysis and cluster analysis. Regression analysis was conducted between reducing sugar/nicotine ratio and sensory quality. The results showed that the mean value of reducing sugar/nicotine ratio was 9.64±3.80 with the coefficient of variation of 39.36%, and the reducing sugar/nicotine ratio of 96.4% of the samples fell in the range of 4.00 to 20.00. With the ascent of altitude, the reducing sugar/nicotine ratio of tobacco increased. Reducing sugar/nicotine ratio significantly differed between tobaccos from different areas and altitudes. Eight tobacco growing counties in Bijie were clustered into three categories on the basis of reducing sugar/nicotine ratio, Zhijin, Qianxi, Nayong and Qixingguan counties belonged to Category I with the reducing sugar/nicotine ratio of 8.39±2.48, Dafang and Jinsha counties were in Category II with the reducing sugar/nicotine ratio of 9.82±4.62, Hezhang and Weining counties in Category III with the reducing sugar/nicotine ratio of 11.59±3.74. In the range of 4.00 to 28.00, the reducing sugar/ nicotine ratio posed an extremely significant regression relationship with sensory quality indicators (except for combustibility). Tobacco offered better sensory quality when its reducing sugar/nicotine ratio was in the range of 8.00-22.00. ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved.


Liu C.,Henan Agricultural University | Zhai X.,Bijie Branch of Guizhou Provincial Tobacco Company | Xu Z.-C.,Henan Agricultural University | Chen X.,Bijie Branch of Guizhou Provincial Tobacco Company | And 3 more authors.
Chinese Journal of Ecology | Year: 2014

We collected samples of soil and tobacco leaves from 109 field sites in Wumeng tobacco-growing area in Guizhou Province, and identified the agro-geological background characteristics of light-sweet flavor style of flue-cured tobacco in terms of soil parent rock, parent material source, soil type and nutrition, and landform. The results indicate that carbonate and clastic sedimentary rocks are mainly the soil parent rocks for the typical light-sweet flavor style, carbonate sedimentary rocks for the light-sweet flavor style, and carbonate and clay sedimentary rocks for the weak light-sweet flavor style. Although the sources and compositions of parent materials that affect the formation of flue-cured tobacco styles are different, they are all characterized as slope sediments. Yellow brown soil and sand land can improve the light-sweet flavor of flue-cured tobacco, and the contents of organic matter, available nitrogen, total potassium, water-soluble chlorine in the soil for the typical light-sweet flavor style are low, while the available potassium content is the highest. Furthermore, the high-middle mountain landform is mainly responsible for the typical light-sweet flavor style, the shares of high-middle and middle mountain landform are equivalent for the light-sweet flavor style, and the middle mountain landform is for the weak lightsweet flavor style. © 2014, Editorial Board of Chinese Journal of Ecology. All rights reserved.

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