Bihār Sharīf, India
Bihār Sharīf, India

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PubMed | Bihar Veterinary College
Type: Journal Article | Journal: Veterinary world | Year: 2016

The objective of this study was to evaluate the effect of season and sex on hemato-biochemical parameters of turkey (Meleagris gallopavo) in the arid tropical environment.The experiment was conducted on 20-week old turkeys consisting of 20 males and 20 females. Blood was collected from all turkeys during January and May. Hemoglobin (Hb), red blood cell (RBC), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were estimated in whole blood and glucose, protein, albumin, globulin, A/G ratio, calcium, phosphorus, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in serum.Season has significant (p<0.05) effect on Hb concentration, RBC, and PCV in both male and female. Male has significantly higher (p<0.05) Hb concentration, RBC, and PCV. There is no significant effect of sex, and season was observed on MCV, MCH, and MCHC. Glucose, protein, albumin, globulin, and A/G ratio were significantly (p<0.05) affected by season and sex. AST and ALT were significantly (p<0.05) affected by season in both sexes. There is no significant difference was recorded on calcium, phosphorus due to season and sex.Under arid tropical environment, turkey hemato-biochemical parameters are influenced by both sex and season.


PubMed | Indian Veterinary Research Institute, Bihar Veterinary College, Central Institute of Brackishwater Aquaculture, Central Institute for Research on Goats and 2 more.
Type: Journal Article | Journal: Iranian journal of microbiology | Year: 2015

Haemorrhagic septicaemia (HS), caused by Pasteurella multocida, is the most important bacterial disease of cattle and buffaloes in India. Oil adjuvant vaccine (OAV) is the most potent vaccine available for the control of HS. The study aims to evaluate the effect of alum co-adjuvantation of OAV on emulsion stability and immune response.Two different oil adjuvant vaccines viz., standard oil adjuvant vaccine (OAV) and alum precipitated oil adjuvant vaccine (A-OAV) were prepared with Pasteurella multocida antigen. Emulsion stability was tested by centrifugation, storage at 37 C for 3 months and microscopy. Immune responses were evaluated by ELISA antibody titer, CD4, CD8 T cell populations and survival post challenge by P. multocida in mice.The separation of aqueous and oil phase of emulsion by centrifugation and storage test were 0 and 6.76% in A-OAV as compared to 11.00 and 26.39% in OAV, respectively. The mean droplet size was significantly smaller (p<0.01) in A-OAV as compared to OAV. The A-OAV recorded higher ELISA antibody titer (p<0.05) up to 21st days post vaccination, and higher CD4 (p>0.05) and CD8 T cell (p<0.05) populations compared to OAV. The A-OAV group conferred 100% protection after challenge with both 100 LD50 and 1000 LD50 as compared to 100 and 60% respective protection by OAV group.The results indicates that A-OAV had better emulsion stability, produces higher level of CD4, CD8 T cells and antibody titer with better protection compared to oil adjuvant vaccine.


Mohan K.,Bihar Veterinary College | Prakash B.S.,National Dairy Research Institute
Tropical Animal Health and Production | Year: 2010

The objective of this study was (1) to establish the duration of behavioral estrus signs and timing of ovulation in Murrah buffaloes (n = 10) and (2) to determine relationship between behavioral estrus signs with change in plasma estrogen concentrations in animals treated with Heatsynch protocol. Estrus and its behavioral signs were detected at hourly intervals by visual observations per rectal examination of genitalia and bull parading four times in a day for 30 min each. Among the behavioral signs of estrus, swollen vulva was the best indicator of estrus followed by excitement and chasing by bull (90%). Among the duration of behavioral estrus signs, the first and longest duration of estrus signs was swollen vulva, which was seen up to 21. 6 ± 1. 1 h after onset of estrus. The mean total duration of estrus symptoms from appearance to disappearance of all the behavioral estrus symptoms was 40. 2 ± 1. 8 h. Endocrine profile during the periestrus period showed that the mean peak concentrations of total estrogen 330. 9 ± 108. 3 pg/ml occurred at 9. 6 ± 1. 0 h after estradiol benzoate injection. The average number of estrus symptoms observed per animal during Heatsynch treatment was 7. 1. Ovulation occurred after 50. 0 ± 2. 0 h after estradiol benzoate treatment and 26. 7 ± 2. 0 h after end of total estrogen surge, respectively. In conclusion, our results suggest that all signs of behavioral estrus occurred after the preovulatory rise in estrogens. The first sign of estrus was swollen vulva, and this symptom persisted the longest. © 2009 Springer Science+Business Media B.V.


Kumari R.R.,Bihar Veterinary College | Kumar P.,DLFM | Mondal T.,West Bengal University of Animal and Fishery Sciences
National Journal of Physiology, Pharmacy and Pharmacology | Year: 2013

Background: Chromium (Cr), a naturally occurring heavy metal, exists in many states, but the trivalent Cr (III) and hexavalent Cr (VI) forms are the most common in the environment. Acute and chronic toxicity in both animal and man are mainly caused by Cr (VI) compounds. Studies on hematological parameters in sub-acute toxicity of potassium chromate in broiler chicks are limited. Aims & Objective: The study was envisaged to evaluate the protective effect of vitamin E and Selenium on haematological parameters in sub-acute oral toxicity of potassium chromate [Cr (VI)] in broiler chicks. Materials and Methods: Potassium chromate (K2CrO4) used as test compound and Vitamin E and Se powder as Tocosel® contained 5 g of Tocopherol acetate and 30 mg of sodium selenite per 10 gm. LD50 was calculated before initiating the experiment by graphical probit analysis method. Experiment was conducted in randomly divided four equal groups (n=6) of day old chicks. Chicks of each group were maintained up to the age of 45 day and sacrificed on 46th day. Chicks of control group (T1) were provided only with basal diet and drinking water. While groups T2 and T3 was fed different concentrations of K2CrO4 and T4 as treatment group supplemented with Vitamin E and Se. Results: LD50 of K2CrO4 in broiler chick was calculated as 277.95 mg/kg. Feeding potassium chromate [Cr (VI)] in group T2 (1/5th of LD50) and T3 (1/3rd of LD50) for 30 days, decreased haemoglobin percentage, haematocrit values, total erythrocyte count and total leucocyte count while increased the clotting time in a dose dependent manner. In group T4 chicks fed 1/3rd of LD50 of K2CrO4 and simultaneous treatment of Vitamin E and Se, normalised these haematological values, signifying its protective effect in sub-acute hexavalent chromium toxicity produced by potassium chromate. Conclusion: It may be concluded that above mentioned changes might have reduced erythrocyte number, leucocyte number, haemoglobin value and increased clotting time. Vitamin E and Se containing glutathione peroxidase are among the principle in vivo chain breaking antioxidants, thus had protective effect.


Tiwary R.,Bihar Veterinary College | Amarpal,Indian Veterinary Research Institute | Aithal H.P.,Indian Veterinary Research Institute | Kinjavdekar P.,Indian Veterinary Research Institute | And 2 more authors.
Cartilage | Year: 2014

Objective: To investigate the utility of bone-marrow-derived mononuclear cells (BMNCs) and insulin like growth factor-1 (IGF-1) in articular cartilage repair. Design: An osteochondral defect of 3 mm diameter and 5 mm depth was created in patellar groove of the left knee joint in each of 36 New Zealand White rabbits. The defect was filled with RPMI-1640 medium in group A (control), autologous BMNCs in group B, and autologous BMNCs plus IGF-1 in group C (n = 12). Healing of the defect was assessed by gross, scanning electron microscopic, radiographic, and histological examinations up to 90 days. Results: Gross and scanning electron microscopic examination of the healing site revealed superior gross morphology and surface architecture of the healing tissue in the animals of group C as compared to other groups. Radiographically on day 90, the defect area was not distinguishable from the surrounding area in group C, but a small circular defect area was still evident in groups A and B. The regenerated tissue was mostly hyaline in group C and fibrocartilage in groups A and B. The cells were well organized and showed better deposition of proteoglycans in groups C and B than in group A. Conclusions: It was concluded that implantation of bone-marrow-derived nucleated cells may facilitate the healing of osteochondral defects; however, the combination of BMNCs and IGF-1 induces faster and histologically better healing than the BMNCs alone. © The Author(s) 2013.


Kumar K.,Bihar Veterinary College | Chaudhary L.C.,Indian Veterinary Research Institute | Agarwal N.,Indian Veterinary Research Institute | Kamra D.N.,Indian Veterinary Research Institute
Tropical Animal Health and Production | Year: 2016

The effect of feeding tannin-degrading bacteria (Streptococcus gallolyticus strain TDGB 406) on carcass characteristics of goats fed with oak (Quercus semicarpifolia) leaves was studied on 18 male goats (4 months old, average body weight 9.50 ± 1.50 kg), distributed into three groups of six animals each. The animals of group 1 served as control, while the animals of groups 2 and 3 were given (at 5 ml/kg live weight) autoclaved and live culture of isolate TDGB 406 (106 cells/ml), respectively. The animals were fed with oak leaves as a basal roughage source and maize hay along with fixed quantity of concentrate mixture. After 4 months of feeding, the animals were slaughtered for carcass studies. The feeding of live culture of isolate TDGB 406 did not cause any effect (P > 0.05) on pre-slaughter weight, empty body weight, carcass weight, dressing percent, and yield of wholesale cuts (neck, rack, shoulder, breast, shank, loin, leg, and flank) of the goat meat. The chemical composition of longissimus dorsi muscle was comparable (P > 0.05) among the groups. The organoleptic evaluation of pressure-cooked meat in terms of tenderness and overall palatability was increased significantly (P < 0.05) in the meat of group 3 where live culture was supplemented. The other attributes were similar among the groups. It was concluded that supplementation of tannin-degrading bacteria S. gallolyticus strain TDGB 406 to goats fed with oak leaves did not affect the carcass characteristics and meat quality. © 2016 Springer Science+Business Media Dordrecht


Ansari M.M.,Sher-e-Kashmir University of Agricultural Sciences and Technology | Sharma S.P.,Bihar Veterinary College
Buffalo Bulletin | Year: 2013

The present experimental urothroplasty was undertaken in sixteen male buffalo calf models apparently in good health status using urinary and caecal grafts. The surgical technique was evaluated on the basis of clinical observation, determination of blood urea nitrogen, histopathological and postoperative complication for a period of 60 days post-operatively. Urethroplasty using formalin preserved urinary bladder and caecal grafts acted as a scaffold around which there was gradual regeneration of urethral tissue and resolution of grafted material by 60th post-operative day. However, both these techniques were worthy and feasible in buffalo calves, yet caecourethroplasty was preferred, because it was easier and safer than the cystourethroplasty.


Bharti S.K.,Bihar Veterinary College | Gautam A.K.,Bihar Veterinary College
Indian Journal of Animal Research | Year: 2013

The study was conducted to elucidate gross and histomorphological structure of magnum in indigenous chicken of Assam. The magnum was abruptly larger and longer than other parts of oviduct and it was most tortuous. The mucosa of magnum was lined by simple columnar ciliated epithelium with primary and secondary folding.The propria-submucosa was packed with long branched coiled tubular glands and it contained large amount of collagen fibres and somewhat less amount of elastic fibres. PAS positive reaction was intense in glands of magnum and the central part of mucosal folds was moderate PAS positive.


Jayachandran C.,Bihar Veterinary College | Nirala R.K.,Bihar Veterinary College | Agrawal A.K.,Bihar Veterinary College
Indian Journal of Animal Sciences | Year: 2014

Pharmacokinetics and testing of dosage regimen of cefazolin was carried out in 4 healthy female buffalo calves after its intravenous (iv) administration @ 10 mg/kg. Plasma and urine concentrations of cefazolin were estimated at various time intervals by microbiological assay using Sarcina lutea (ATCC 9341). From log plasma concentrations versus time, kinetics parameters were calculated. Based on kinetic parameters, loading (D∗) and maintenance (D0) doses were calculated for maintaining CP∞ min (MIC) of 0.5 μg/ml at the dosage interval (γ) of 6h. Using the data, appropriate D∗ was injected i/v and 3 consecutive Do were given at selected dosage interval (γ) of 6 h to maintain CP∞ min of 0.5 μg/ml in each animal. The study obtained distribution (t1/2 β) and elimination (t1/2 β) half life, volume distribution, and total body clearance (ClB) of 0.19±0.06 h, 4.78±0.28 h, 5.49±0.83 L/ kg and 13.15±1.53 ml/kg/min. D∗ and D0 of 6.52±0.77 and 3.77±0.38 mg/were calculated to maintain CP∞ min of 0.5 μg/ml at γ of 6 h.


PubMed | Indian Veterinary Research Institute and Bihar Veterinary College
Type: Journal Article | Journal: Cartilage | Year: 2015

To investigate the utility of bone-marrow-derived mononuclear cells (BMNCs) and insulin like growth factor-1 (IGF-1) in articular cartilage repair.An osteochondral defect of 3 mm diameter and 5 mm depth was created in patellar groove of the left knee joint in each of 36 New Zealand White rabbits. The defect was filled with RPMI-1640 medium in group A (control), autologous BMNCs in group B, and autologous BMNCs plus IGF-1 in group C (n = 12). Healing of the defect was assessed by gross, scanning electron microscopic, radiographic, and histological examinations up to 90 days.Gross and scanning electron microscopic examination of the healing site revealed superior gross morphology and surface architecture of the healing tissue in the animals of group C as compared to other groups. Radiographically on day 90, the defect area was not distinguishable from the surrounding area in group C, but a small circular defect area was still evident in groups A and B. The regenerated tissue was mostly hyaline in group C and fibrocartilage in groups A and B. The cells were well organized and showed better deposition of proteoglycans in groups C and B than in group A.It was concluded that implantation of bone-marrow-derived nucleated cells may facilitate the healing of osteochondral defects; however, the combination of BMNCs and IGF-1 induces faster and histologically better healing than the BMNCs alone.

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