BIGTEC PRIVATE Ltd | Date: 2011-04-28
The present disclosure provides a non contact real time micro Polymerase Chain Reaction [PCR] system comprises; a chip having a reaction chamber for holding a sample and an embedded metal heater below the reaction chamber for heating the sample; an optical unit comprising associated LED driver and photo detector amplifier placed above the chip to detect the fluorescence; an induction heater mounted around the chip and is inductively coupled to the metal heater; an infrared temperature sensor mounted below the chip for measuring a temperature of the metal heater, wherein said infrared temperature sensor is interfaced with a signal conditioner; and a controller interfaced with the signal conditioner and the induction heater for regulating the power to the induction heater based on feedback received from the infrared temperature sensor through the signal conditioner.
BIGTEC PRIVATE Ltd | Date: 2011-07-15
The present disclosure gives description of a method used for the detection and quantification of dengue viral infection caused by dengue virus using nucleic acids isolated from blood, plasma or serum samples by employing Oligonucleotide probes. The method employed here for detection is by Real time PCR. The instant disclosure also provides for primers, probes, PCR Reaction mixture and kit thereof.
BIGTEC PRIVATE Ltd | Date: 2010-01-27
The present disclosure provides a method for the detection and quantification of Hepatitis B Virus. It discloses oligonucleotide probes set forth in SEQ ID Nos. 1 and 2 for detection of Hepatitis B Virus along with respective primers [sense and antisense] set forth in SEQ ID Nos. 3, 4, 5 and 6. It also provides a PCR reaction mixture for detection of Hepatitis B Virus and a kit for detection of HBV comprising said mixture along with an instruction package.
BigTec Private Ltd | Date: 2010-01-27
The present disclosure gives description of a method used for the detection and quantification of malarial infection caused either by
BIGTEC PRIVATE Ltd | Date: 2010-02-23
The present disclosure gives a detailed description of methods for determining the presence of Chikungunya viral nucleic acids in blood/serum/plasma samples by employing Oligonucleotide probes. The designed Oligonucleotide probes can be used for qualitative or quantitative detection of Chikungunya virus in an infected sample by employing Real time PCR.