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Bhagat S.,Indian Agricultural Research Institute | Pan S.,Bidhan Chandra Krishi Viswavidyalaya Mohanpur
Biopesticides International

Twelve isolates of Trichoderma spp. were evaluated for their competitive parasitic ability against sclerotia of Sclerotium rolfsii by using as live baits under pot condition. The present investigation revealed that withdrawal of stasis barrier (fungistasis/mycostasis) from soil by means of either sun drying or steam sterilization gradually reduced the EID 50 values of each isolate of Trichoderma. The mycelial or chlamydospore form of inocula had lower EID 50 values than conidial form of inocula. The isolates ThrWB-1 and ThrWB-2 were found most effective in sclerotial parasitization of S. rolfsii under Mohanpur (WB) soils, whereas they performed very poorly in Port Blair soil. However, the isolates ThrWB-1, ThrAN-5, ThrAN-7, TvAN-3 and TvAN-5 were most promising isolates with high competitive colonization of sclerotia of S. rolfsii under both soils. The lowest effective inoculum dose for 50% colonization of sclerotia of Rhizoctonia solani was recorded with chlamydospore form of inoculum of ThrAN-5 (EID 50 = 2.33) followed by TvAN-5 (EID 50 = 2.81) and TvAN-3 (EID 50 = 2.90) under the sterilized soil of Port Blair. But these isolates of Trichoderma required much higher EID 50 values (4.72, 4.95 and 4.92) in natural soil of Mohanpur. Lowest collar rot incidence in brinjal was noted with ThrWB-1 (T 3, seed + soil application) and highest reduction in disease incidence (77.1%) was also recorded with seed and soil application of same isolate followed by TvAN-3 (T 12), ThrAN-5 (T 6), TvAN-5 (T 15), whereas the isolate ThrAN-13 was least effective. Source

Pramanick B.,Bidhan Chandra Krishi Viswavidyalaya Mohanpur | Brahmachari K.,Bidhan Chandra Krishi Viswavidyalaya Mohanpur | Ghosh A.,Indian Central Salt and Marine Chemicals Research Institute | Zodape S.T.,Indian Central Salt and Marine Chemicals Research Institute
Bangladesh Journal of Botany

On summer rice, foliar spray was applied thrice at different concentrations (0, 2.5, 5.0, 7.5, 10.0 and 15.0%, v/v) of seaweed extracts (namely, Kappaphycus sp. and Gracilaria sp.) along with the recommended dose of fertilizer (RDF). The highest grain yield was recorded with the applications of 15% Kappaphykus sap + RDF, followed by 15% Gracilaria sap + RDF extract resulting in 41.47 and 34.99% increases, respectively compared to the control. The maximum straw yield was also achieved with 15% seaweed extract application. Improved nutrient uptake (N, P and K) was also observed with seaweed extract applications. Source

Biswas T.,Bidhan Chandra Krishi Viswavidyalaya Mohanpur | Bhattacharya S.,Bidhan Chandra Krishi Viswavidyalaya Mohanpur
Indian Journal of Agricultural Research

Twenty eight diverse rice genotypes collected from West Bengal, have been studied to assess genetic diversity using eight SSR markers linked with QTL of drought tolerance. All the SSR markers generated two reproducible alleles except RM-300 where three alleles were observed. The percentage of highest frequency allele among the used SSR markers ranging from 57.14 % to 100 %. PIC value was higher in case of RM-231, RM-278 and RM-490 therefore these markers could be used for diversity analysis. For most of the markers rare alleles were detected in Kalonunia, Satika, Shungakalma, Turkibalam, Laldhula and in Asnala. So, these genotypes are suitable for marker assisted breeding with the aim of increasing drought tolerance. Dendogram revealed 5 clusters at 0.65 % similarity coefficient level. Polymorphism identified by 8 SSR markers and resultant diversity analysis of the Bengal landraces may serve in selecting parental combination as well as in marker assisted breeding programme. Source

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