Granada, Spain

Time filter

Source Type

Carrasco-Marin E.,Hospital Santa Cruz Of Liencres | Rodriguez-Del Rio E.,Hospital Santa Cruz Of Liencres | Frande-Cabanes E.,Hospital Santa Cruz Of Liencres | Tobes R.,BIC Granada - CEEI | And 6 more authors.
Journal of Biological Chemistry | Year: 2012

Phagosomes are critical compartments for innate immunity. However, their role in the protection against murine listeriosis has not been examined. We describe here that listericidal phago-receptosomes are induced by the function of IFN-γ or IL-6 as centralized compartments for innate and adaptive immunity because they are able to confer protection against murine listeriosis. These phago-receptosomes elicited LLO(91-99)/CD8+- and LLO(189-201)/CD4+-specific immune responses and recruited mature dendritic cells to the vaccination sites controlled by T cells. Moreover, they present exceptional features as efficient vaccine vectors. First, they compartmentalize a novel listericidal STAT-1-mediated signaling pathway that confines multiple innate immune components to the same environment. Second, they show features of MHC class II antigen-loading competent compartments for cathepsin-D-mediated LLO processing. Third, murine cathepsin-D deficiencies fail to develop protective immunity after vaccination with listericidal phago-receptosomes induced by IFN-γ or IL-6. Therefore, it appears that the connection of STAT-1 and cathepsin-D in a single compartment is relevant for protection against listeriosis. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.


Rio E.R.,Fundacion Marques Of Valdecilla Ifimav And Hospital Santa Cruz Of Liencres Humv | Frande-Cabanes E.,Fundacion Marques Of Valdecilla Ifimav And Hospital Santa Cruz Of Liencres Humv | Tobes R.,BIC Granada - CEEI | Pareja E.,BIC Granada - CEEI | And 4 more authors.
International Journal of Biochemistry and Molecular Biology | Year: 2011

LLO is the major immuno-dominant antigen in listeriosis and is also required for protective immunity. Two forms of LLO can be observed in endosomal membranes, a LLO intact form and a Ctsd-processed LLO1-491 form. En-dosomes obtained from resting macrophages contained only LLO intact forms, while endosomes obtained from IFN-activated macrophages contained both forms. Both types of endosomes elicited LLO90-91/CD8 + and LLO189-201/CD4 + specific immune responses. However, only endosomes containing the Ctsd-processed LLO 1-491 form showed significant CD4 + and CD8 + T cell responses similar to LM infected bone marrow derived macrophages and characteristic of protective Listeria immunity. Moreover, endosomes with intact LLO could not confer protection as vaccine carriers against murine listeriosis. While endosomes with Ctsd-processed LLO1-491 form showed a moderate ability, slightly lower than high efficiency vaccine vectors as MØ infected with LM. These studies argue that all cell-free membrane vesicles might serve as valid vaccine carriers against infectious agents. Exclusively those cell-free vesicles MIIC competent for LLO processing are protective vaccines vectors since they recruit significant numbers of mature dendritic cells to the vaccination sites and contain a LLO 1-491 form that might be accessible for MHC class I and class II antigen presentation.


BIC Granada - CEEI | Entity website


BIC Granada - CEEI | Entity website


BIC Granada - CEEI | Entity website

Qu son las cookies? Una cookie es un fichero que se descarga en tu ordenador al acceder a determinadas pginas web. Las cookies permiten a una pgina web, entre otras cosas, almacenar y recuperar informacin sobre los hbitos de navegacin de un usuario o de su equipo, gestionar el acceso de usuarios a zonas restringidas de la web, etc ...


BIC Granada - CEEI | Entity website


BIC Granada - CEEI | Entity website

BIC Granada es un Centro Europeo de Empresas e Innovacin perteneciente a la Agencia de Innovacin y Desarrollo de Andaluca, organismo dependiente de la Consejera de Empleo, Empresay Comerciode la Junta de Andaluca. Contamos con 12aos de experiencia en la creacin y desarrollo de Empresas Innovadoras de Base Tecnolgica, (EIBTs)


BIC Granada - CEEI | Entity website

BIC Granada es un Centro Europeo de Empresas e Innovacin perteneciente a la Agencia de Innovacin y Desarrollo de Andaluca, organismo dependiente de la Consejera de Empleo, Empresay Comerciode la Junta de Andaluca. Contamos con 12aos de experiencia en la creacin y desarrollo de Empresas Innovadoras de Base Tecnolgica, (EIBTs)


BIC Granada - CEEI | Entity website

Loading BIC Granada - CEEI collaborators
Loading BIC Granada - CEEI collaborators