Biberach an der Riss, Germany

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Patent
Biberach University of Applied Sciences | Date: 2015-04-24

The invention relates to a nucleic acid construct comprising at least two different regions, wherein the regions are selected of a first region encoding for at least one miRNA stimulating cellular production of a biomolecule in a cell, a second region encoding for at least one miRNA and/or miRNA-inhibitor suppressing cell death, and a third region encoding for at least one miRNA and/or miRNA-inhibitor regulating cell proliferation. The invention further relates to a cell comprising a respective nucleic acid construct and to a method for increasing the yield of a biomolecule produced by a cell cultured in vitro comprising at least two steps selected of stimulating cellular production of the biomolecule, reducing cell death, and regulating proliferation.


Lochner I.,Biberach University of Applied Sciences
Structures and Architecture - Proceedings of the 3rd International Conference on Structures and Architecture, ICSA 2016 | Year: 2016

This paper presents studies for the design of structures on the basis of methods of structural optimization, especially topology optimization based on the homogenization method. These tools are widely applied in automotive and aircraft design, while their application in the field of architectural design is still marginal. The benefits of these methods can be found both in objective engineering as well as in aesthetic design solutions: these tools merge aspects of architectural design and engineering, resulting in both aesthetic and reasonable structures. © 2016 Taylor & Francis Group, London.


Kaysser L.,University of Tübingen | Siebenberg S.,University of Tübingen | Kammerer B.,Biberach University of Applied Sciences | Gust B.,University of Tübingen
ChemBioChem | Year: 2010

En route: The liposidomycin biosynthetic gene cluster has been identified, cloned and heterologously expressed. A comparison with the gene cluster of the structurally related caprazamycins supports the proposed pathway to liponucleoside formation and led to the identification of new sulfated caprazamycin derivatives. (Chemical Equation Presented). © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.


Henze G.P.,University of Colorado at Boulder | Floss A.G.,Biberach University of Applied Sciences
Energy and Buildings | Year: 2011

The degradation of the temperature difference between supply and return flow reduces the efficiency of chilled water systems as well as central heating systems. In both applications, the problem is caused by excessive water flow rates under part load conditions and equipment oversizing. Remedies to address this ΔT degradation by reducing bypass flow have been recommended and documented over the last 25 years, especially in the field of chilled water systems. This paper addresses ΔT degradation caused by a set of common faults: hydraulic network imbalance, control valve oversizing, incorrect inherent valve characteristic, and inadequate control loop gain parameters. To allow for the quantitative evaluation of the numerous possible causes that contribute to ΔT degradation, a dynamic simulation environment was developed and employed in the presented analysis that couples pressure-flow relationships in the hydraulic network with the dynamic thermal loads of the building under investigation. Relative to the reference case of a properly designed and tuned hydraulic subsystem, a ΔT degradation of over 6 K in a sample central heating system is predicted, well aligned with commissioning reports of large chilled water and district heating plants. In district heating systems, this significant ΔT degradation of 6 K would lead to an increase of the primary energy consumption between 4 and 12%. © 2011 Elsevier B.V. All rights reserved.


Paul A.J.,Biberach University of Applied Sciences | Schwab K.,Biberach University of Applied Sciences | Hesse F.,Biberach University of Applied Sciences
BMC Biotechnology | Year: 2015

Background: Protein aggregation during monoclonal antibody (mAb) production can occur in upstream and downstream processing (DSP). Current methods to determine aggregate formation during cell culture include size exclusion chromatography (SEC) with a previous affinity chromatography step in order to remove disturbing cell culture components. The pre-purification step itself can already influence protein aggregation and therefore does not necessarily reflect the real aggregate content present in cell culture. To analyze mAb aggregate formation directly in the supernatant of Chinese hamster ovary (CHO) cell culture, we established a protocol, which allows aggregate quantification using SEC, without a falsifying pre-purification step. Results: The use of a 3 μm silica SEC column or a SEC column tailored for mAb aggregate analysis allows the separation of mAb monomer and aggregates from disturbing cell culture components, which enables aggregate determination directly in the supernatant. Antibody aggregate analysis of a mAb-producing CHO DG44 cell line demonstrated the feasibility of the method. Astonishingly, the supernatant of the CHO cells consisted of over 75% mAb dimer and larger oligomers, representing a substantially higher aggregate content than reported in literature so far. Conclusion: This study highlights that aggregate quantification directly in the cell culture supernatant using appropriate SEC columns with suitable mAb aggregate standards is feasible without falsification by previous affinity chromatography. Moreover, our results indicate that aggregate formation should be addressed directly in the cell culture and is not only a problem in DSP. © 2014 Paul et al.; licensee BioMed Central Ltd.


Fischer S.,Biberach University of Applied Sciences | Fischer S.,University of Ulm | Handrick R.,Biberach University of Applied Sciences | Aschrafi A.,Radboud University Nijmegen | Otte K.,Biberach University of Applied Sciences
RNA Biology | Year: 2015

Understanding the multifaceted nature of microRNA (miRNA) function in mammalian cells is still a challenge. Commonly accepted principles of cooperativity and multiplicity of miRNA function imply that individual mRNAs can be targeted by several miRNAs whereas a single miRNA may concomitantly regulate a subset of different genes. However, there is a paucity of information whether multiple miRNAs regulate critical cellular events and thereby acting redundantly. To gain insight into this notion, we conducted an unbiased high-content miRNA screen by individually introducing 1139 miRNA mimics into Chinese hamster ovary (CHO) cells. We discovered that 66% of all miRNAs significantly impacted on proliferation, protein expression, apoptosis and necrosis. In summary, we provide evidence for a substantial degree of redundancy among miRNAs to maintain cellular homeostasis. © 2015 Taylor & Francis Group, LLC.


Moreth J.,Biberach University of Applied Sciences | Mavoungou C.,Biberach University of Applied Sciences | Schindowski K.,Biberach University of Applied Sciences
Immunity and Ageing | Year: 2013

Alzheimer's disease (AD) is the most common dementia in the industrialized world, with prevalence rates well over 30% in the over 80-years-old population. The dementia causes enormous costs to the social healthcare systems, as well as personal tragedies for the patients, families and caregivers. AD is strongly associated with Amyloid-beta (Aβ) protein aggregation, which results in extracellular plaques in the brain, and according to the amyloid cascade hypothesis appeared to be a promising target for the development of AD therapeutics. Within the past decade convincing data has arisen positioning the soluble prefibrillar Aβ-aggregates as the prime toxic agents in AD. However, different Aβ aggregate species are described but their remarkable metastability hampers the identification of a target species for immunization. Passive immunotherapy with monoclonal antibodies (mAbs) against Aβ is in late clinical development but recently the two most advanced mAbs, Bapineuzumab and Solanezumab, targeting an N-terminal or central epitope, respectively, failed to meet their target of improving or stabilizing cognition and function. Preliminary data from off-label treatment of a small cohort for 3 years with intravenous polyclonal immunoglobulins (IVIG) that appear to target different conformational epitopes indicate a cognitive stabilization. Thus, it might be the more promising strategy reducing the whole spectrum of Aβ-aggregates than to focus on a single aggregate species for immunization. © 2013 Moreth et al.; licensee BioMed Central Ltd.


Moreth J.,Biberach University of Applied Sciences | Mavoungou C.,Biberach University of Applied Sciences | Schindowski K.,Biberach University of Applied Sciences
Frontiers in Aging Neuroscience | Year: 2013

Amyloid-beta (Aβ) in Alzheimer's disease (AD) appeared to be a promising target for disease-modifying therapeutic strategies like passive immunotherapy with anti-Aβ monoclonal antibodies (mAbs). Biochemical markers in cerebrospinal fluid (CSF) include alterations of Aβ that allow the diagnosis of AD. Biomarker strategies, such as the levels of Aβ in CSF and plasma, currently play an important role in early clinical trials for AD. Indeed, these strategies have a relevant impact on the outcome of such studies, since the biomarkers are used to monitor the bioactivity of anti-Aβ mAbs. The clinical trials of Solanezumab were mainly based on the readout of Aβ levels in CSF and plasma, whereas those of Bapineuzumab were based on cognition; however, little is known about the mechanisms altering these biomarker levels, and no biomarker has yet been proven to be a successful predictor for AD therapy. In addition, the Aβ biomarkers allow for the determination of free and bound anti-Aβ mAb in order to monitor the available amount of bioactive drug and could give hints to the mechanism of action. In this review, we discuss clinical Aβ biomarker data and the latest regulatory strategies. © 2013 Moreth, Mavoungou and Schindowski.


Patent
Biberach University of Applied Sciences | Date: 2015-10-28

The invention relates to a nucleic acid construct comprising at least two different regions, wherein the regions are selected of a first region encoding for at least one miRNA stimulating cellular production of a biomolecule in a cell, a second region encoding for at least one miRNA and/or miRNA-inhibitor suppressing cell death, and a third region encoding for at least one miRNA and/or miRNA-inhibitor regulating cell proliferation. The invention further relates to a cell comprising a respective nucleic acid construct and to a method for increasing the yield of a biomolecule produced by a cell cultured in vitro comprising at least two steps selected of stimulating cellular production of the biomolecule, reducing cell death, and regulating proliferation.


Patent
Biberach University of Applied Sciences | Date: 2016-03-23

In a method for evaluating the energy efficiency of a refrigeration machine (1; 2) and/or a heat pump (3; 4), wherein a working fluid of the refrigeration machine (1; 2) and/or heat pump is running through an expansion valve (40; 40A, 40B, 40C) at one point of a thermodynamic cycle, at different positions of the thermodynamic cycle, at least one thermodynamic parameter (T) of the working fluid is detected. The mass flow (m) of the working fluid is determined using a classification (m) of the opening of the expansion valve (40; 40A, 40B, 40C) and some of the detected thermodynamic parameters (T) of the working fluid. A cooling power and/or a heating power (Q) of the refrigeration machine (1; 2) and/or heat pump (3; 4) is determined as a function of the mass flow (m) of the working fluid. The energy efficiency of the refrigeration machine and/or heat pump (3; 4) is evaluated by comparing the cooling power and/or heating power (Q) to an electrical power (P used to power the refrigeration machine and/or heat pump.

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