Bhojia Institute of Life science

Baddi, India

Bhojia Institute of Life science

Baddi, India
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Kumar A.,Bhojia Institute of Life science | Chopra J.,Bhojia Institute of Life science | Singh S.K.,U For Life | Khan A.,U For Life | And 2 more authors.
Der Pharmacia Lettre | Year: 2015

Azo dyes account for most of all textile dyes stuffs produced and have been the most commonly used synthetic dyes in textile, food, paper making and cosmetic industries. Release of azo dyes into the environment from the effluents of dye- utilizing industries has become a major concern in wastewater treatment. Some azo dyes have been linked to human bladder cancer, spleenic sarcomas, hepato-carcinomas & chromosomal aberrations in mammalian cells. Bacillus subtilis 'RA29' (NCBI accession no: JF901735) was applied on four different azo dyes (Congo red, Amido black, Acid orange & Rhodamine B) for the decolorization. In Yeast Malt broth (YMB), Bacillus subtilis 'RA29' showed 98.23% decolorization of Congo red, 78.32% Amido black, 96.69% Acid orange & 6.69% Rhodamine B at pH 7.0 after 20 hr of incubation at 37°C. During the growth and Congo red decolorization kinetic study, the maximum decolorization (99.7%), cell yield (347.80) with biomass concentration 17.216mg/ml was observed after 16 hr of incubation at temperature 37°C under static aerobic condition. The enzyme activities of laccase, azoreductase, peroxidase enzymes in partially purified extracellular crude protein were detected as 7.34U/ml, 0.168 U/ml, 0.134 U/ml respectively. Liquid chromatography mass spectroscopy (LCMS) studies detected the tentative presence of AcetoAcet-P-ChloroAnilide, 3-(2,2-dichlorovinyl)-2,2-dimethyl cyclopropane-1-carboxylic acid chloride, N-{4-[(4-Bromophenyl) sulfamoyl] phenyl}-N2-[(4-chlorophenyl) sulfonyl]-N2 (2 ethoxyphenyl) glycinamide and 2, 2, 2-Trichloroethanol at retention time 1.571 min, 13.68 min, 19.153 min and 18.234 min respectively.


Kumar A.,Bhojia Institute of Life science | Chopra J.,Bhojia Institute of Life science | Sharma A.,Bhojia Institute of Life science
Current Trends in Biotechnology and Pharmacy | Year: 2014

The various plant parts (stem, leaf, bark, fruit and sap) extract of Ficus benghalensis had been known for their anathematic, analgesic, anti-inflammatory, antioxidants, antidiabetic, immuno-modulatory and antimicrobial activity for ancient time. These plant part extracts are used in various ayurvedic medicines to treat diarrhea, dysentery and piles, teeth disorders, rheumatism, skin disorders like sores and to boost immune system. The present study was focused on the prop root extracts (hydro & ethanolic) of F. benghalensis against the pure bacterial strains of human oral cavity which inhibits gingival and supragingival diseases and was also analyzed for the bioactive phytochemicals compounds. The qualitative analysis of the F. benghalensis prop roots crude hydroextract revealed the presence of flavonoids, saponins, steroids and reducing sugars while ethanolic extracts showed the presence of alkaloids, tannins, and glycosides additionally. The Liquid Chromatography Mass Spectroscopy (LCMS) showed presence of α- ethyl-α-phenyl glutarimide, 1, 2- Dihexanoyl-sn- Glycero-3-Phosphocholine, Antimony trisulfide, 2, 2, 2-trichloroethanol, Antimony trioxide at retention time of 1.586 min., 12.377 min.,13.646 min., 14.376 min., and 22.185 min respectively in crude hydroextract of prop roots of F. benghalensis. The Thin Layer Chromatography (TLC) product as bioactive compound was identified as 2-chloroethyl phosphonic acid. The antibacterial activity of crude hydroextract were also determined and found the encouraging results against bacterial strain Streptococcus sp. (8 mm zone of inhibition), Lactobacillus sp. (9 mm), Actinobacillus sp. (7 mm), Fusobacterium sp. (9 mm) but TLC separated product (2- chloroethyl phosphonic acid) showed antibacterial activity only against Lactobacillus sp. as 17 mm zone of inhibition. © 2014 Association of Biotechnology and Pharmacy. All rights reserved.


About 50 bacterial strains, each of Pseudomonas fluorescens, from different rhizospheric soil of different plants were screened for antagonistic activity against Curvularia lunata, Fusarium oxysporum, Alternaria padwickii, Rhizoctonia solani causing black kernel, kernel spotting, root rots, stackburn and sheath blight diseases of rice (Oryza sativa L.). Out of the 50 isolates, 15 isolates were found to be effective in lysing the cell wall of the above-mentioned putative pathogens tested in vitro. These Pseudomonas isolates produced mycolytic enzymes, viz. β-1,3-glucanases, β-1,4-glucanases and lipases. P. fluorescens PAK1 and PAK12 among the strains were more effective for the production of these enzymes while PAK12 produce good level of β-1,3-glucanases, β-1,4-glucanases and lipases against tested fungal pathogens. These findings demonstrate a mechanism of antagonism by P. fluorescens against different fungal plant pathogens. © 2011 Taylor & Francis.


Karnwal A.,Bhojia Institute of Life science | Kaushik P.,Gurukula Kangri Vishwavidyalaya
Archives of Phytopathology and Plant Protection | Year: 2011

Free-living bacteria may trigger the plant growth through production of phytohormones viz. gibberellins, auxins and cytokinins. A total 50 isolates of fluorescent Pseudomonas were screened for their ability to produce cytokinins such as isopentenyladenosine (IPA), dihydroxyzeatin riboside (DHZR) and zeatin riboside (ZR) as plant growth-promoting activity. Pseudomonas fluorescens AK1 and Pseudomonas aeruginosa AK2 were found higher phytohormones producing strains. Of the three cytokinins, IPA was the major cytokinin produced by both isolates in pure culture (5.5 and 2.9 pmol/ml, respectively) and with rice root exudates (5.9 and 3.4 pmol/ml, respectively). Production of ZR and DHZR for both organisms was found after 48 and 72 h. Amount of ZR and DHZR increased with time for both isolates in pure culture conditions. In presence of rice, production of ZR was increased 0.8 and 0.6 pmol/ml for P. fluorescence AK1 and P. aeruginosa AK2, respectively, in comparison with controls. There was no significant difference in the production of DHZR with rice exudates. © 2011 Taylor & Francis.


Singh R.,Bhojia Institute of Life science | Mutneja R.,Panjab University | Kaur V.,Panjab University | Wagler J.,TU Bergakademie Freiberg | Kroke E.,TU Bergakademie Freiberg
Journal of Organometallic Chemistry | Year: 2013

Some new silatranes containing an azomethine group as coordinating system in the axial chain are reported. This system is introduced into the chain by the treatment of 3-aminopropylsilatrane with different carbonyl compounds like acetylacetone (1), 2-acetylphenol (2), paeonol (3) and salicylaldehyde (4) in the presence of anhydrous sodium sulphate. All the compounds were characterized by elemental analysis, spectroscopic techniques and X-ray diffraction analysis. The thermal stability of all silatranes was studied by TGA/DTG/DSC techniques, which is in agreement with the Mass spectrometry and X-ray diffraction studies. © 2012 Elsevier B.V. All rights reserved.


Sawhney R.,Bhojia Group of Institutions | Kumar A.,Bhojia Institute of Life science | Robina,Bhojia Group of Institutions | Jayata,Bhojia Group of Institutions
Asian Journal of Microbiology, Biotechnology and Environmental Sciences | Year: 2014

Congo red, a carcinogenic diazo dye is discharged through industrial effluents in the environment. The removal of this hazardous dye from effluents is important for health and environment protection. In recent past, immobilization has come up as a promising biological tool. The present study highlights the immobilization of Bacillus subtilis sub sp. subtilis RA-29' on a suitable matrix and the potential of immobilized whole cells to decolourize congo red. The whole cell immobilized calcium alginate beads were stable for 7 days. The immobilized RA-29 exhibited cell density dependent decolourization. Maximum congo red decolourization (81 .55%) was achieved at cell density of 3Oug/mm3 after 48 hr of incubation at 37°C under static condition. The calcium alginate immobilized "RA-29" showed operational stability with efficient decolourization up to five cycles with minimum decolourization percentage falling to 61.11%. The immobilized Bacillus subtilis could be commercially viable biotechnological tool for bioremediation of dye contaminated industrial effluents. © Global Science Publications.

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