Bhavans College andheri West

Mumbai, India

Bhavans College andheri West

Mumbai, India
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Shetti P.,Bhavans College andheri West | Venkatachalam A.,Bhavans College andheri West
International Journal of PharmTech Research | Year: 2011

Donepezil is used worldwide for the treatment of Alzheimer's disease, where it is used to increase cortical acetylcholine. We present a simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method developed and validated for the quantification of Donepezil in Human Plasma. Quetiapine was used as the internal standard. The simple and cost effective Liquid-Liquid Extraction method is applied for the extraction of Donepezil and Internal standard from Plasma. A rapid isocratic separation of Donepezil is achieved by a short C18 column using mobile phase of Acetonitrile and 1mM Ammonium acetate buffer (92:8 v/v, (%)) at flow rate of 0.5 ml/min. The run time is 2.5 minutes suggests high throughput of the proposed method. The collision-induced transition m/z 380 à 91 was used to analyze donepezil in selected reaction monitoring mode. The signal intensity of this transition was found to relate linearly with donepezil concentrations in plasma from 0.1- 42 ng/ml. The lower limit of quantification of the LC-MS/MS method was 0.1ng/ml. The intra-and inter-day precisions were below 10.0% and the accuracy was between + 5.0% and - 5.0%. The validated LC/MS/MS method was applied to pharmacokinetic study on healthy male volunteer who received single oral dose of 10 mg Donepezil hydrochloride. The maximum plasma concentration observed was 17.05ng/ml at 2.5 hours post dosing. This validated LC-MS/MS is rapid, sensitive, specific and cost-effective method for determining donepezil in human plasma samples.


Sanap R.,D 5 2 | Mirza Z.,Bhavans College andheri West
Acta Zoologica Lituanica | Year: 2011

The trapdoor spider genus Scalidognathus Karsch, 1891 is represented in India by a single species, S. montanus Pocock, 1900 from Shevaroy hills in Tamil Nadu. The specimens collected during a recent survey conducted in the southern Western Ghats of Tamil Nadu enable us to describe two new species of the genus Scalidognathus. Natural history notes and burrow structure of both species are provided.


Singare P.U.,Bhavans College andheri West | Lokhande R.S.,University of Mumbai
Ionics | Year: 2012

The present paper deals with the study of iodide and bromide ion-isotopic exchange reactions by application of radioactive tracer technique. The specific reaction rate (per minute) of both the reactions decreases with rise in temperature and increases with concentration of ionic solution. It was observed that due to solvation effect, the iodide ion-isotopic exchange reaction take place at a faster rate than bromide ion-isotopic exchange reaction. Also for iodide ion-isotopic exchange reaction, the distribution coefficient (K d) values were higher than that for bromide ion-isotopic exchange reaction. The application of tracer technique was further extended for characterization of two widely used nuclear grade ion exchange resins Tulsion A-33 and Duolite ARA 9366. It was observed that the values of K d, specific reaction rate (per minute), amount of ion exchanged (millimoles), and percentage of ions exchanged for Tulsion A-33 resins are higher than that for Duolite ARA 9366 resins under identical experimental conditions. © 2011 Springer-Verlag.


Singare P.U.,Bhavans College andheri West
Russian Journal of Physical Chemistry A | Year: 2014

Radioanalytical technique using 131I and 82Br was employed to evaluate organic based anion exchange resins Tulsion A-30 and Indion-930A. The evaluation was based on performance of these resins during iodide and bromide ion-isotopic exchange reactions. It was observed that for iodide ion-isotopic exchange reaction by using Tulsion A-30 resin, the values of specific reaction rate (min-1), amount of iodide ion exchanged (mmol), initial rate of iodide ion exchange (mmol/min) and logK d were 0.238, 0.477, 0.114, and 11.0, respectively, which was higher than 0.155, 0.360, 0.056, and 7.3, respectively as that obtained by using Indion-930A resins under identical experimental conditions of 40.0°C, 1.000 g of ion exchange resins and 0.003 M labeled iodide ion solution. Also at a constant temperature of 40.0°C, as the concentration of labeled iodide ion solution increases 0.001 to 0.004 M, for Tulsion A-30 resins the percentage of iodide ions exchanged increases from 59.0 to 65.1%, and from 46.4 to 48.8% for Indion-930A resins under identical experimental conditions. The identical trend was observed for both the resins during bromide ion-isotopic exchange reactions. The overall results indicate that under identical experimental conditions, Tulsion A-30 show superior performance over Indion-930A resins. The results of present experimental work have demonstrated that the radioanalytical technique used here can be successfully applied for characterization of different ion exchange resins so as to evaluate their performance under various process parameters. © 2014 Pleiades Publishing, Ltd.


Shetti P.,Bhavans College andheri West | Venkatachalam A.,Bhavans College andheri West
E-Journal of Chemistry | Year: 2010

A new, simple, precise, rapid, selective and stability indicating reversed-phase high performance liquid chromatographic (HPLC) method has been developed and validated for simultaneous quantification of trihexyphenidyl hydrochloride, trifluoperazine hydrochloride and chlorpromazine hydrochloride from combined tablet formulation. The method is based on reverse-phase using C-18 (250x4.6) mm, 5 μm particle size column. The separation is achieved using isocratic elution by methanol and ammonium acetate buffer (1% w/v, pH 6.5) in the ratio of 85:15 v/v, pumped at flow rate 1.0 mL/min and UV detection at 215 nm. The column is maintained at 30 °C through out the analysis. This method gives baseline resolution. The total run time is 15 min. Stability indicating capability is established buy forced degradation experiment. The method is validated for specificity, accuracy, precision and linearity as per International conference of harmonisation (ICH). The method is accurate and linear for quantification of trihexyphenidyl hydrochloride, trifluoperazine hydrochloride and Chlorpromazine hydrochloride between 5 - 15 μg/mL, 12.5- 37.5 μg/mL and 62.5 - 187.5 μg/mL respectively.


Patil R.C.,Bhavans college andheri West | Murugkar T.P.,Bhavans college andheri West | Shaikh S.A.,Bharati Vidyapeeth Deemed University
International Journal of Pharma and Bio Sciences | Year: 2012

Degradation of waste especially that of vegetable waste has become a mounting challenge owing to its generation in huge amounts. This vegetable market generated waste can be utilized to biosynthesize pectinase enzyme at cheaper rate which have numerous industrial applications. Screening was carried out to obtain pectinase producing microorganisms from carrot. The best isolate was identified as a unique strain of Bacillus sp. based on morphological, biochemical tests and using 16S rRNA gene sequencing studies. This isolate was selected for further bulk production using the optimized conditions of pH 9 and temperature of 50°C to give a maximum yield of 49.58% from carrot waste.


Khanna M.M.,Bhavans College andheri West
Journal of Advanced Microscopy Research | Year: 2014

Soft slimy bodies of Molluscans are protected, supported and sheltered under their hard calcareous shell. This abode which is available for retreat in case of threats is of a peculiar shape in each of the genera and species of molluscans. Scutus (=Parmaphorus) unguis (Linnaeus) was selected for the morphological and analytical studies as this valuable information would contribute to Taxonomy and Mineralogy. Mineralogy of the fossil, recent and for that matter a primitive shell may be useful in Palaeoecological, Palaeoenvironmental, Palaeogeological, Petroleum geological, Phylogeny, Pollution and taxonomical studies. Comparison of the composition of fossil and recent molluscan shells can be used to study the differences in their composition and the attribute to the effects of weathering and diagenesis. Shell of Scutus unguis has been studied using SEM (Scanning Electron Microscopy) and EDX analysis. Shell surface architecture reveals that the protoconch of Scutus unguis is paucispiral and trochispiral type. The umbilicus is imperforate and the protoconch is retained at the apex. The surface is found to be uniformly lamellated and is correlated to the very sluggish slow moving behavior of the animal. Interlamellar regions are characterized by the presence of the very fine denticles. Lead and Zinc have been detected in the shell of Scutus unguis which was collected from the polluted rocky shores of Girgaum Chowpatty, Mumbai, India. Copyright © 2014 American Scientific Publishers All rights reserved.


The paper deals with the application of radio isotopic non-destructive technique in the characterization of two industrial grade anion exchange resins Indion GS-300 and Indion-860. For the characterization of the two resins, 131I and 82Br were used as tracer isotopes to trace the kinetics of iodide and bromide ion-isotopic exchange reactions. It was observed that the values of specific reaction rate (min-1), amount of iodide ion exchanged (mmol), initial rate of iodide ion exchange (mmol/min) and log Kd were calculated as 0.328, 0.577, 0.189 and 19.7 respectively for Indion GS-300 resin, which was higher than the respective values of 0.180, 0.386, 0.070 and 17.0 calculated for Indion-860 resins when measured under identical experimental conditions. Also at a constant temperature of 40.0 °C, as the concentration of labeled iodide ion solution increases 0.001 M to 0.004 M, the percentage of iodide ions exchanged increases from 75.16% to 78.36% for Indion GS-300 resins, which was higher than the increases from 49.65% to 52.36% compared to that obtained for Indion-860 resins. The overall results indicate that under identical experimental conditions, Indion GS-300 resins show superior performance over Indion-860 resins.


Singare P.U.,Bhavans College Andheri West
Colloid Journal | Year: 2014

The present study deals with the kinetic study of iodide and bromide ion-isotopic exchange reactions in organic based anion exchange resins Indion-102 (nuclear grade) and Indion GS-400 (non-nuclear grade) using radiotracer isotopes. The resins in iodide and bromide form were equilibrated respectively with iodide and bromide ion solutions which were previously spiked with 131I and 82Br radiotracer isotopes. For both bromide and iodide ion-isotopic exchange reactions, it was observed that the values of specific reaction rate increase with increase in ion concentration from 0.001 to 0.004 M at a constant temperature of 40.0°C. However, at constant ion concentration of 0.003 M, the specific reaction rate was observed to decrease with rise in temperature from 30.0 to 45.0°C. Also it was observed that for iodide ion-isotopic exchange reaction by using Indion-102 resin, the values of specific reaction rate, amount of iodide ion exchanged, initial rate of iodide ion exchange and logKd were 0.258 min−1, 0.492 mmol, 0.127 mmol/min and 19.2, respectively, which were higher than 0.208 min−1, 0.416 mmol, 0.087 mmol/min and 17.6, respectively, obtained by using Indion GS-400 resin under identical experimental conditions of 40.0°C, 1.000 g of ion exchange resin and 0.003M labeled iodide ion solution. The same trend was observed for the two resins during bromide ion-isotopic exchange reaction. The overall results indicate that, under identical experimental conditions, Indion-102 resin shows higher performance than Indion GS-400 resin. © 2014, Pleiades Publishing, Ltd.


Singare P.U.,Bhavans College Andheri West
Journal of Radioanalytical and Nuclear Chemistry | Year: 2014

The present study deals with characterization of industrial grade anion exchange resins Amberlite IRN78 and Indion H-IP for which non-destructive radiotracer technique using 131I and 82Br was used. The radioisotopes were used to trace the kinetics of iodide and bromide ion-isotopic exchange reactions taking place in the two resins. It was observed that under identical experimental conditions of 40.0 C, 1.000 g of ion exchange resins and 0.003 M labeled iodide ion solution for iodide ion-isotopic exchange reaction, the values of specific reaction rate (min-1), amount of iodide ion exchanged (mmol), initial rate of iodide ion exchange (mmol min-1) and log K d were 0.285, 0.544, 0.155 and 12.6 respectively for Amberlite IRN78 resin, which was higher than 0.093, 0.315, 0.029 and 4.9 respectively as that obtained by using Indion H-IP resins. Also at a constant temperature of 40.0 C, as the concentration of labeled iodide ion solution increases 0.001-0.004 M, the percentage of iodide ions exchanged increases from 68.10 to 74.00 % for Amberlite IRN78 resin, which was higher than the increase of 40.20-42.80 % as observed for Indion H-IP resins. The identical trend was observed for the two resins during bromide ion-isotopic exchange reaction. The overall results indicate that that under identical experimental conditions Amberlite IRN78 resins shows superior performance over Indion H-IP resins. © 2013 Akadémiai Kiadó, Budapest, Hungary.

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