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Mohan Goud V.,Joginpally Br Pharmacy College | Rao A.S.,Bhaskar Pharmacy College
Der Pharma Chemica | Year: 2011

The present work is carried out for the synthesis of benzimidazole derivatives with well established procedures. A new series of benzimidazole derivatives were synthesized and the structures of these compounds were confirmed by IR, HNMR and Mass Spectroscopy. The title compounds were screened for analgesic and anti inflammatory activities by biological evaluation method and also for other possible pharmacological activities including antibacterial activity. Among the synthesized compounds, some have shown promisingly significant analgesic, anti-inflammatory activites and moderate antibacterial activity.


Vishnu Priya P.,Joginpally Brpharmacy College | Radhika K.,Joginpally Brpharmacy College | Srinivasa Rao A.,Bhaskar Pharmacy College
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2011

Synthetic drugs are potentially toxic and are not free of side effects on the host, an attempt has been made to study the anti-oxidant activity on plants. As plants and plant-based drugs are less toxic and have acceptable side effects. The crude extracts of leaves of Parthenium hysterophorus were selected for studying anti-oxidant activity. The plant extracts were prepared by using solvents (methanol, acetone and ethanol) and the activity was determined by DPPH (2, 2-diphenyl-1-picrylhydrazyl radical) scavenging assay with vitamin E as standard. By DPPH assay it was shown that acetone extract has higher anti-oxidant activity than methanol and chloroform extracts. The medicinal plants with anti-oxidant properties leading to possess good anti-cancer activities. The anti-oxidant phytochemicals protect the cells from oxidative damage caused by free radicals. Thus, consuming a diet rich in anti-oxidant foods (e.g. fruits and vegetables) will provide health-protective effects. It is a significance to exploit novel anti-oxidant drugs from the medicinal plants.


Hadi M.A.,Bhaskar Pharmacy College | Rao N.G.R.,Jyothishmathi Institute of Pharmaceutical Science | Rao A.S.,Bhaskar Pharmacy College
Pakistan Journal of Pharmaceutical Sciences | Year: 2015

In this present research work, we have designed a pulsincap formulation comprising mini-tablets, which to the best of our knowledge this combination has not been reported yet. We successfully combined the advantages of minitablets technology to meet the optimized requirements of our pulsincap formulation. Our main aim was to target lornoxicam to treat rheumatoid arthritis as per the chronotherapeutic pattern of the disease. Directly compressing method was used to prepare mini-tablets. The drug, polymers and combine mixtures of drug and polymers was evaluated for preformulation testing. Prepared mini-tablets were also evaluated for physicochemical, dissolution and stability studies. From FTIR and DSC evaluation, we found no interaction between the drug and polymers used. For mini-tablets, all the physico-chemical parameters were in limit. The mini-tablets of lornoxicam were filled into an insoluble body of capsule, and its opening was sealed by plugging it with a polymer. The complete capsule body after sealing with a cap was given enteric coating. Different polymers in various concentrations were used as a plug, to identify the most suitable which gives a complete lag time of 5 hours when combined with 5% CAP coating. HPMC-K100M in 30% and sodium alginate in 40% concentrations were identified as the most suitable plugs. Our optimized pulsincap formulations releases lornoxicam after a lag time of 5 hrs and maximum portion of the drug will be released in the early morning hours. It was also found to be stable for a period of 6 months as per ICH guidelines.


Runja C.,Joginpally Br Pharmacy College | Ravi Kumar P.,Research Solutions | Avanapu S.R.,Bhaskar Pharmacy College
Journal of Chromatographic Science | Year: 2016

A new simple, rapid stability indicating assay method has been developed and validated for the determination of emtricitabine, tenofovir disoproxil fumarate, elvitegravir and cobicistat using reverse-phase high-performance liquid chromatography in their pharmaceutical dosage form. The chromatographic separation was performed on an ODS column (250 × 4.6 mm, 5 μm) using mobile phase A (potassium dihydrogen orthophosphate, pH adjusted to 2.5) and mobile phase B (acetonitrile) in the ratio of 55:45% v/v at a flow rate of 1 mL/min. The analytes were detected at 250 nm. The method was found to be linear in the concentration range of 2-12 μg/mL for EMT, 3-18 μg/mL for TNDF, 1.5-9 μg/mL for ELV and COB, with the coefficient value (R2) of >0.9990. The accuracy was measured via recovery studies and found to be acceptable, and the percentage recoveries were found in the range of 99.93-100.08 ± 0.5%. Forced degradation studies were also conducted, and the drugs were subjected to various stress conditions such as acid hydrolysis, base hydrolysis, oxidative, photolytic and thermal degradation. The proposed method was successfully validated and applied for the quantitative estimation of these drugs in both bulk and tablet dosage forms. © 2016 The Author 2016. Published by Oxford University Press. All rights reserved.


PubMed | Research Solutions, Joginpally Br Pharmacy College and Bhaskar Pharmacy College
Type: Journal Article | Journal: Journal of chromatographic science | Year: 2016

A new simple, rapid stability indicating assay method has been developed and validated for the determination of emtricitabine, tenofovir disoproxil fumarate, elvitegravir and cobicistat using reverse-phase high-performance liquid chromatography in their pharmaceutical dosage form. The chromatographic separation was performed on an ODS column (250 4.6 mm, 5 m) using mobile phase A (potassium dihydrogen orthophosphate, pH adjusted to 2.5) and mobile phase B (acetonitrile) in the ratio of 55:45% v/v at a flow rate of 1 mL/min. The analytes were detected at 250 nm. The method was found to be linear in the concentration range of 2-12 g/mL for EMT, 3-18 g/mL for TNDF, 1.5-9 g/mL for ELV and COB, with the coefficient value (R(2)) of >0.9990. The accuracy was measured via recovery studies and found to be acceptable, and the percentage recoveries were found in the range of 99.93-100.08 0.5%. Forced degradation studies were also conducted, and the drugs were subjected to various stress conditions such as acid hydrolysis, base hydrolysis, oxidative, photolytic and thermal degradation. The proposed method was successfully validated and applied for the quantitative estimation of these drugs in both bulk and tablet dosage forms.


Vishnu Priya P.,Joginpally Br Pharmacy College | Srinivasa Rao A.,Bhaskar Pharmacy College
Asian Journal of Pharmaceutical and Clinical Research | Year: 2015

Objective: Evaluation of anticancer activity of various extracts of leaves of Tridax procumbens by 3-(4,5-dimethyl-thiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, and trypan blue dye exclusion assay against A549 (human lung cancer cell line), Hep G2 (human liver carcinoma cell line). Methods: In vitro anticancer activity of ethanol, acetone, and aqueous leaf extracts of T. procumbens was evaluated on selected cancerous cells lines by MTT assay and trypan blue dye exclusion assay. MTT assay is based on the capacity of mitochondrial enzymes of viable cells to reduce the yellow soluble salt MTT to purple blue insoluble formazan precipitate which is then quantified spectrophotometrically at 570 nm. Trypan blue assay is based on staining of cells. Cells are then counted using hemocytometer under the microscope, non-viable cells were stained blue, viable cells remain unstained. Results: The aqueous leaf extract of T. procumbens has not shown any anticancer activity. However, potent anticancer activity was shown by the acetone and ethanol leaf extracts of T. procumbens on A549 (human lung cancer cell line), Hep G2 (human liver carcinoma cell line). Conclusion: The anticancer medicinal plant i.e., T. procumbens was studied by in vitro evaluation methods i.e., MTT assay and trypan blue exclusion assay. The acetone and ethanol leaf extract of T. procumbens have shown potent anticancer activity on selected cancerous cell lines. More efforts are needed to explore potent anticancer plants from the mother earth and save humans around the world from cancer. © 2015, Asian Journal of Pharmaceutical and Clinical Research. All rights reserved.


PubMed | Joginpally Br Pharmacy College and Bhaskar pharmacy college
Type: | Journal: Annales pharmaceutiques francaises | Year: 2016

A liquid chromatography tandem mass spectrometry (LC-MS/MS) based method was developed for the simultaneous estimation of pioglitazone and its active metabolites in human plasma for applicability to pharmacokinetic studies. The chromatographic separation was carried on the reversed phase Peerless Basic C18, column (1004.6mm, 5m) at column temperature of 40C using a binary mobile phase consisting of methanol: 5mM ammonium acetate in 0.1% formic acid (80:20, v/v). The mobile phase was run at a flow rate of 1mL/min and the sample injection was 10L. The method utilized pioglitazoneD4 (IS1) and 5-hydroxyl pioglitazone M-IVD4 (IS2) as an internal standard. The linearity of the method was validated over the range of 6.04-1503.21ng/mL for pioglitazone and 6.01-1496.28ng/mL for 5-hydroxyl pioglitazone. The mean extraction recovery of PIO & HPIO from the spiked plasma was found to be 94.92% for pioglitazone and 96.13% for 5-hydroxy pioglitazone. The developed method can be successfully employed in healthy human volunteers to monitor the pharmacokinetics profile of pioglitazone.


Srinivasa Rao A.,Bhaskar Pharmacy College | Ahmed M.F.,Nizam Institute of Pharmacy and Research Center | Ibrahim M.,Nizam Institute of Pharmacy and Research Center
Journal of Applied Pharmaceutical Science | Year: 2012

The aim of the study is to investigate the hepatoprotective activity of Melia azedarach L leaves extracts against simvastatin induced hepatotoxicity. The phytochemical screening was carried on the leaves extracts of Melia azedarach revealed the presence of some active ingredients such as Alkaloids, Tannins, Sponginess, Phenols, glycosides, steroids, terpenoids and flavonoids. Leaves of Melia azedarach was successively extracted with ethanol against simvastatin (20mg/kg.p.o) induced hepatotoxicity using Standard drug Silymarin (25 mg/kg). There was a significant changes in biochemical parameters (increases in serum glutamate pyruvate transaminase (SGPT), Serum glutamate oxaloacetate transaminase (SGOT), alanine phosphatase (ALP),serum bilirubin and decrease the total proteins content.) in simvastatin treated rats, which were restored towards normalization in Melia azedarach (300 mg/kg and 500 mg/kg) treated animals. Thus the present study ascertains that the leaf extract of Melia azedarach possesses significant hepatoprotective activity.


PubMed | Bhaskar Pharmacy College and Moonray Institute of Pharmaceutical science
Type: Journal Article | Journal: Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society | Year: 2016

In this present research work, the aim was to develop ileo-colonic targeted matrix-mini-tablets-filled capsule system of Naproxen for chronotherapeutic treatment of Rheumatoid Arthritis. So Matrix-mini-tablets of Naproxen were prepared using microsomal enzyme dependent and pH-sensitive polymers by direct compression method which were further filled into an empty HPMC capsule. The compatibility was assessed using FT-IR and DSC studies for pure drug, polymers and their physical mixtures. The prepared batches were subjected to physicochemical studies, drug content estimation, in-vitro drug release and stability studies. When FTIR and DSC studies were performed, it was found that there was no interaction between Naproxen and polymers used. The physicochemical properties of all the prepared matrix-mini-tablets batches were found to be in limits. The drug content percentage in the optimized formulation F18 was found to be 99.240.10%. Our optimized matrix-mini-tablets-filled-capsule formulation F18 releases Naproxen after a lag time of 2.450.97h and 27.300.86%, 92.590.47%, 99.380.69% at the end of 5, 8, 12h respectively. This formulation was also found to be stable as per the guidelines of International Conference on Harmonisation of Technical Requirements of Pharmaceuticals for Human Use. Thus, a novel ileo-colonic targeted delivery system of Naproxen was successfully developed by filling matrix-mini-tablets into an empty HPMC capsule shell for targeting early morning peak symptoms of rheumatoid arthritis.


Cherukuvada S.,University of Hyderabad | Bolla G.,University of Hyderabad | Sikligar K.,University of Hyderabad | Sikligar K.,Bhaskar Pharmacy College | Nangia A.,University of Hyderabad
Crystal Growth and Design | Year: 2013

4-Aminosalicylic acid (p-aminosalicylic acid, PAS), an antituberculosis drug, is a model active pharmaceutical ingredient to study salt and cocrystal formation in a multiple hydrogen-bonding functionality molecule with carboxylic acid, amine, and phenol groups. A cytosine salt CYT+-PAS-, salt cocrystal hydrate CYT+-PAS--CYT-H2O, and nicotinamide cocrystal hydrate PAS-NAM-H2O, are described in this article. Furthermore, X-ray crystal structures of PAS sodium dihydrate, sulfate, and mesylate salts and dehydration/rehydration behavior of the sodium salt by powder X-ray diffraction are discussed. © 2013 American Chemical Society.

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