Bhaskar Pharmacy College

Hyderabad, India

Bhaskar Pharmacy College

Hyderabad, India
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Kumar K.S.,Jawaharlal Nehru University | Rao A.S.,Bhaskar Pharmacy College
Asian Journal of Pharmaceutical and Clinical Research | Year: 2017

Objective: This investigation involves the extraction, isolation, and characterization of flavonoid from a Euphorbiaceae family plant Chrozophora plicata followed by evaluation of its antioxidant principles. Methods: The dried leaves were subjected to sequential soxhlation with polar and nonpolar solvents. Methanolic extract reveals the presence of large amount of flavonoids. Methanolic extract was subjected to isolation using column chromatographic analysis with solvents such as petroleum ether, chloroform, hexane, ethyl acetate, methanol, and water. Further, the isolated compound was subjected to thin layer chromatography technique and spectral analysis such as infrared,1HNMR,13CNMR, mass spectroscopy, and high performance thin layer chromatography (HPTLC) finger printing techniques. The compound was evaluated for in vitro antioxidant studies using 2,2-diphenyl-1-picrylhydrazyl (DPPH), NO assay, reducing power assay, H2O2 scavenging assay, superoxide anion scavenging assay and β-Carotene linoleate system and in vivo antioxidative studies using carbon tetrachloride (CCl4), and acetaminophen intoxicated rats. Results: The compound was isolated in methanol:water in the ratio of 80:20 using column chromatographic technique. On the basis of phytochemical, chromatographic, and spectral analysis, the isolated compound was identified as kaempferol and finally with HPTLC finger printing technique it was found that the Rf value of the isolated compound was found to be 0.58 which is nearly similar to the Rf value of standard kaempferol (0.55). Hence, the isolated compound was confirmed as kaempferol and is structurally elucidated as 3,5,7-trihydroxy-2-(4-hydroxyphenyl)chromen-4-one. This compound was isolated for the first time from the C. plicata leaves. The in vitro antioxidant assay of isolated flavonoid has shown a dose-dependent increase in free radical scavenging activity using DPPH, no assay, reducing power assay, H2O2 scavenging assay, superoxide anion scavenging assay, and β-carotene linoleate system. Further, the methanolic extract of C. plicata (MECP) leaves was subjected to single dose acute toxicity study for 14 days in female rats on the basis of OECD guidelines 423 and the therapeutically selected doses were 200 mg/kg and 400 mg/kg. In vivo antioxidant studies in CCl4 and acetaminophen intoxicated rats indicated that the MECP leaves have significantly decreased lipid peroxidation in a dose-dependent manner and increased the levels of catalase, superoxide dismutase, and glutathione. Conclusions: By the above results, it was concluded that the isolated compound from C. plicata leaves was confirmed as kaempferol and it possesses significant antioxidative potentials. © 2017 The Authors.


Ahmed M.F.,Nizam Institute of Pharmacy and Research Center | Srinivasa Rao A.,Bhaskar Pharmacy College
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2014

Objective: The present study was carried out to estimate the phenolics compound, Rutin, Quercetin and Kaempferol in Brassica oleracea L.var capitata. by high-performance liquid chromatography (HPLC). Methods: For the estimation of Rutin, Quercetin and Kaempferol by HPLC method, the mobile phase used are, Acetonitrile and Phosphate buffer (pH=5.8) in ratio of 55: 45.Quantification of Rutin, Quercetin and Kaempferol was carried by Athena C18 column and absorbance was measured at 254 nm with flow rate of 1 ml/min. Results: In HPLC analysis the retention time(Rt) of standards, Rutin, Quercetin and Kaempferol were found to be 2.357, 6.093 and 9.373 respectively, while the Retention times of Rutin and Kaempferol in Brassica oleracea L.var capitata are 2.387, 6.060 and 9.113 which are found to be matching with standards retention time values respectively. Conclusion: Thus this HPLC method was found to be simple and rapid for quantitative determination of Rutin, Quercetin and Kaempferol in Brassica oleracea L.var capitata.


Anjali Devi N.,Joginpally B R Pharmacy College | Hadi M.A.,Bhaskar Pharmacy College | Rajitha P.,Bhaskar Pharmacy College | Sharma J.V.C.,Joginpally B R Pharmacy College | Srinivasa Rao A.,Bhaskar Pharmacy College
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2013

Imatinib Mesylate is an anti cancer agent which is used to treat chronic myelogenous leukemia (CML), gastrointestinal stromal tumors (GISTs) and a number of other malignancies. In the present research work an attempt has been made for the formulation of floating tablet containing Imatinib Mesylate as a drug candidate which would remain in stomach or upper part of GIT for prolonged period of time thereby maximizing the drug release at the desired site within the stipulated time. In the present study Imatinib Mesylate floating tablets were prepared by wet granulation method. The floating tablets were subjected to preformulation studies, in-vitro drug release, kinetic studies and stability studies. FTIR studies shown there was no interaction between drug and polymers. The percentage of Imatinib Mesylate content from the tablets was determined by UV-Spectroscopy and ranged from 98. 25±1. 8 to 98. 91±1. 5. The in-vitro percentage release of Imatinib Mesylate from the optimized tablets at the end of 12 hours was 99. 46%. The kinetic studies revealed that the drug was released by zero-order kinetics. The optimized formulation was subjected to stability studies and shown there were no significant changes in drug content, physicochemical parameters and release pattern. From this study, it is concluded that, the formulations retained for longer period of time in the stomach and provides controlled release of the drug. Hence, it will be increasing the bioavailability of the drug and patient compliance.


Priya P.V.,Joginpally Br Pharmacy College | Rao A.S.,Bhaskar Pharmacy College
International Journal of Pharmaceutical Sciences Review and Research | Year: 2016

The present study was aimed to evaluate the anticancer activity of various leaf extracts of Mazus pumilus against PC 3 (prostate cancer cell lines), A549 (lung adeno carcinoma cell line), Hep G2 (liver carcinoma cell line). MTT assay is based on the capacity of mitochondrial enzymes of viable cells to reduce the yellow soluble salt MTT i.e., [3-(4, 5-dimethyl –thiazole-2-yl)-2, 5-diphenyl tetrazolium bromide] to purple blue insoluble formazan precipitate which is then quantified spectrophotometrically at 560 nm. Tryphan blue assay is based on staining of cells. The cells which exclude the stain are viable. The acetone and ethanol leaf extracts of Mazus pumilus has shown potent anticancer activity on A549 and Hep G2 human cancerous cell lines by MTT assay and Tryphan blue dye exclusion test. The aqueous, ethanol and acetone leaf extracts of Mazus pumilus has shown less anticancer activity on PC 3 cancer cell line by MTT assay and Tryphan blue exclusion assay. The results of the study shown that various leaf extracts of Mazus pumilus has high potential of anticancer activity on A549 and Hep G2 human cancerous cell line. Further detailed investigation of active components of the plant for exact mechanism of action will contribute greatly to the development of new pharmaceuticals. © 2016, Global Research Online. All rights reserved.


Rao V.,Cross Roads | Hadi M.A.,Bhaskar Pharmacy College
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2013

Objective: Pregabalin is used for treating pain caused by neurologic diseases such as neuralgias as well as seizures. The starting recommended dose for postherpetic neuralgia is dosing at 75 mg two times a day or 50 mg three times a day (150 mg/day). The half-life of pregabalin is 5-6.5 hrs. So, in order to improve the half-life and bioavailability we have designed twice daily mini-tablets formulation of pregabalin. Method: The system comprises of 15 matrix mini-tablets weighing 25 mg encapsulated in HPMC capsule (size1). For achieving the sustain release profile, various viscosity grades of Hydroxy propyl methyl cellulose polymer (HPMC K4M, K15M, K100M) were used. The mini-tablets were prepared by direct-compression method. The prepared mini-tablets were subjected for pre-compressional and post-compressional parameters. The compatibility of drug with other ingredients was checked by FTIR studies. Stability study carried out as per ICH guidelines for three months. Results: The values of pre-compression parameters evaluated were within prescribed limits and indicated good free flowing property. All the post-compressional parameter evaluated were within acceptable limits. The in-vitro performance of our best mini-tablets formulation showed the desired behavior, nearly 99.57 % of drug was sustained for a period of 12 hrs. FTIR results revealed that there was no interaction between dug and other excipients. The stability study revealed that the formulations were found to be stable. Conclusion: From this, study it can be concluded that, matrix mini-tablets of pregabalin along with HPMC can be used to improve its half-life and improve its bio-availability.


Hadi M.A.,Bhaskar Pharmacy College | Raghavendra Rao N.G.,Moonray Institute of Pharmaceutical science | Srinivasa Rao A.,Bhaskar Pharmacy College
Saudi Pharmaceutical Journal | Year: 2016

In this present research work, the aim was to develop ileo-colonic targeted matrix-mini-tablets-filled capsule system of Naproxen for chronotherapeutic treatment of Rheumatoid Arthritis. So Matrix-mini-tablets of Naproxen were prepared using microsomal enzyme dependent and pH-sensitive polymers by direct compression method which were further filled into an empty HPMC capsule. The compatibility was assessed using FT-IR and DSC studies for pure drug, polymers and their physical mixtures. The prepared batches were subjected to physicochemical studies, drug content estimation, in-vitro drug release and stability studies. When FTIR and DSC studies were performed, it was found that there was no interaction between Naproxen and polymers used. The physicochemical properties of all the prepared matrix-mini-tablets batches were found to be in limits. The drug content percentage in the optimized formulation F18 was found to be 99.24 ± 0.10%. Our optimized matrix-mini-tablets-filled-capsule formulation F18 releases Naproxen after a lag time of 2.45 ± 0.97 h and 27.30 ± 0.86%, 92.59 ± 0.47%, 99.38 ± 0.69% at the end of 5, 8, 12 h respectively. This formulation was also found to be stable as per the guidelines of International Conference on Harmonisation of Technical Requirements of Pharmaceuticals for Human Use. Thus, a novel ileo-colonic targeted delivery system of Naproxen was successfully developed by filling matrix-mini-tablets into an empty HPMC capsule shell for targeting early morning peak symptoms of rheumatoid arthritis. © 2015 The Authors.


Hadi M.A.,Bhaskar Pharmacy College | Rao N.G.R.,Jyothishmathi Institute of Pharmaceutical Science | Rao A.S.,Bhaskar Pharmacy College
Pakistan Journal of Pharmaceutical Sciences | Year: 2015

In this present research work, we have designed a pulsincap formulation comprising mini-tablets, which to the best of our knowledge this combination has not been reported yet. We successfully combined the advantages of minitablets technology to meet the optimized requirements of our pulsincap formulation. Our main aim was to target lornoxicam to treat rheumatoid arthritis as per the chronotherapeutic pattern of the disease. Directly compressing method was used to prepare mini-tablets. The drug, polymers and combine mixtures of drug and polymers was evaluated for preformulation testing. Prepared mini-tablets were also evaluated for physicochemical, dissolution and stability studies. From FTIR and DSC evaluation, we found no interaction between the drug and polymers used. For mini-tablets, all the physico-chemical parameters were in limit. The mini-tablets of lornoxicam were filled into an insoluble body of capsule, and its opening was sealed by plugging it with a polymer. The complete capsule body after sealing with a cap was given enteric coating. Different polymers in various concentrations were used as a plug, to identify the most suitable which gives a complete lag time of 5 hours when combined with 5% CAP coating. HPMC-K100M in 30% and sodium alginate in 40% concentrations were identified as the most suitable plugs. Our optimized pulsincap formulations releases lornoxicam after a lag time of 5 hrs and maximum portion of the drug will be released in the early morning hours. It was also found to be stable for a period of 6 months as per ICH guidelines.


Vishnu Priya P.,Joginpally Br Pharmacy College | Srinivasa Rao A.,Bhaskar Pharmacy College
Asian Journal of Pharmaceutical and Clinical Research | Year: 2015

Objective: Evaluation of anticancer activity of various extracts of leaves of Tridax procumbens by 3-(4,5-dimethyl-thiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, and trypan blue dye exclusion assay against A549 (human lung cancer cell line), Hep G2 (human liver carcinoma cell line). Methods: In vitro anticancer activity of ethanol, acetone, and aqueous leaf extracts of T. procumbens was evaluated on selected cancerous cells lines by MTT assay and trypan blue dye exclusion assay. MTT assay is based on the capacity of mitochondrial enzymes of viable cells to reduce the yellow soluble salt MTT to purple blue insoluble formazan precipitate which is then quantified spectrophotometrically at 570 nm. Trypan blue assay is based on staining of cells. Cells are then counted using hemocytometer under the microscope, non-viable cells were stained blue, viable cells remain unstained. Results: The aqueous leaf extract of T. procumbens has not shown any anticancer activity. However, potent anticancer activity was shown by the acetone and ethanol leaf extracts of T. procumbens on A549 (human lung cancer cell line), Hep G2 (human liver carcinoma cell line). Conclusion: The anticancer medicinal plant i.e., T. procumbens was studied by in vitro evaluation methods i.e., MTT assay and trypan blue exclusion assay. The acetone and ethanol leaf extract of T. procumbens have shown potent anticancer activity on selected cancerous cell lines. More efforts are needed to explore potent anticancer plants from the mother earth and save humans around the world from cancer. © 2015, Asian Journal of Pharmaceutical and Clinical Research. All rights reserved.


Srinivasa Rao A.,Bhaskar Pharmacy College | Ahmed M.F.,Nizam Institute of Pharmacy and Research Center | Ibrahim M.,Nizam Institute of Pharmacy and Research Center
Journal of Applied Pharmaceutical Science | Year: 2012

The aim of the study is to investigate the hepatoprotective activity of Melia azedarach L leaves extracts against simvastatin induced hepatotoxicity. The phytochemical screening was carried on the leaves extracts of Melia azedarach revealed the presence of some active ingredients such as Alkaloids, Tannins, Sponginess, Phenols, glycosides, steroids, terpenoids and flavonoids. Leaves of Melia azedarach was successively extracted with ethanol against simvastatin (20mg/kg.p.o) induced hepatotoxicity using Standard drug Silymarin (25 mg/kg). There was a significant changes in biochemical parameters (increases in serum glutamate pyruvate transaminase (SGPT), Serum glutamate oxaloacetate transaminase (SGOT), alanine phosphatase (ALP),serum bilirubin and decrease the total proteins content.) in simvastatin treated rats, which were restored towards normalization in Melia azedarach (300 mg/kg and 500 mg/kg) treated animals. Thus the present study ascertains that the leaf extract of Melia azedarach possesses significant hepatoprotective activity.


Cherukuvada S.,University of Hyderabad | Bolla G.,University of Hyderabad | Sikligar K.,University of Hyderabad | Sikligar K.,Bhaskar Pharmacy College | Nangia A.,University of Hyderabad
Crystal Growth and Design | Year: 2013

4-Aminosalicylic acid (p-aminosalicylic acid, PAS), an antituberculosis drug, is a model active pharmaceutical ingredient to study salt and cocrystal formation in a multiple hydrogen-bonding functionality molecule with carboxylic acid, amine, and phenol groups. A cytosine salt CYT+-PAS-, salt cocrystal hydrate CYT+-PAS--CYT-H2O, and nicotinamide cocrystal hydrate PAS-NAM-H2O, are described in this article. Furthermore, X-ray crystal structures of PAS sodium dihydrate, sulfate, and mesylate salts and dehydration/rehydration behavior of the sodium salt by powder X-ray diffraction are discussed. © 2013 American Chemical Society.

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