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Mali G.V.,Bharali Vidyapeeths M.B.S.K. Kanya Mahavidyalaya | Patil R.C.,Bharatiya Vidya Bhavans College andheri W | Bodhankar M.G.,P.A. College
Research Journal of Chemistry and Environment | Year: 2011

Azotobacter chroococcum isolates from the rhizosphere of groundnut varieties from different localities were tested for their ability to produce antifungal metabolites and phytohormones - indol acetic acid (IAA) and gibberellins (GA). Out of the 25 isolates, KG 2, KG 3 and KG 5 were found to be more significant as compared with others. KG 2 exhibited activity against Aspergillus flavus, Aspergillus terms and Fusarium oxysporum and produced indol acetic acid and gibberellins in 55 μg/ml and 50 μg/ml amount respectively ; KG 3 exhibited activity against Aspergillus flavus and Aspergillus terms and produced IAA and GA in 56 μg/ml and 58 μg/ml amount respectively whereas KG 5 exhibited activity against Aspergillus terms, Alternaria alternata and Fusarium oxysporum and produced IAA and GA in 53 μg/ml and 62 μg/ml amount respectively. A study on the effect of mixed culture inoculation of these three isolates of Azotobacter chroococcum and native isolates of Rhizobia on nodulation and dry mass of groundnut (Arachis hypogea L.) var. TPG 41, JL 286 and JL 501 was undertaken in pot culture experiment by using randomized block design. A significant increase in nodulation and dry mass of each variety was observed due to mixed culture inoculation over uninoculated control. Treatment with Azotobacter alone or Rhizobium alone and with mixture of Azotobacter and Rhizobium biofertilizers also increased nodulation and dry mass over control but it was less than the native species. Therefore, these isolates could be used as bifertilizers and biocontrol agents. Source

Patil R.C.,Bharatiya Vidya Bhavans College andheri W | Mali G.V.,Bharati Vidyapeeths M.B.S.K. Kanya Mahavidyalaya | Waghela C.B.,Bharatiya Vidya Bhavans College andheri W | Nambiar R.A.,Bharatiya Vidya Bhavans College andheri W | And 3 more authors.
Journal of Pure and Applied Microbiology | Year: 2011

Bio-enhancers are molecules without drug activity that augment the bioavailability of drugs in combination therapy. The aim of this study was to assess the bio-enhancing activity of bioactive fractions obtained from plant extracts on commonly used antibiotics. The effect of ethyl acetate extract of Cleome rutidosperma leaves on the antibacterial activities of amoxycillin and cloxacillin against Staphylococcus aureus NCIM 2614 and Pseudomonas aeruginosa NCIM 2653, respectively, was studied. Moreover, the antimicrobial activities of extracts of different parts of C. rutidosperma were evaluated against S. aureus, P. aeruginosa, Klebsiella pneumoniae, Salmonella typhi, Candida albicans, and Aspergillus niger. Hot ethyl acetate leaves extract (HEALE) and antibiotics were tested individually and in combination. HEALE showed maximum activity against test organisms; the minimum inhibitory concentration (MIC) of HEALE was 600 μg/mL. The test organisms were found to be multidrug-resistant (MDR) strains. However, HEALE or HEALE in combination with antibiotics did not lead to the development of resistance. Phytochemical and chromatographic analyses of HEALE revealed the presence of antimicrobial and bio-enhancing constituents. Such findings herald the possibility of a potentially active bio-enhancer of plant origin. Source

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