Bharathi Womens College Autonomous

Chennai, India

Bharathi Womens College Autonomous

Chennai, India
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Megala J.,Bharathiar University | Megala J.,Vels University | Geetha A.,Bharathi Womens College Autonomous
Pharmacologyonline | Year: 2010

The study was designed to evaluate the gastroprotective effect of hydroalcoholic fruit extract of Pithecellobium dulce (HAEPD) in the injury of rat gastric mucosa induced by absolute ethanol and as well as to elucidate the role of reactive oxygen species, lipid peroxidation and some important antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GSH-Px) and myeloperoxidase (MPO) in these effects as markers of ulceration process following oral administration of HAEPD and Omeprazole in rats with absolute ethanol-induced ulcers. HAEPD (200 mg/ kg b wt) as well as Omeprazole (30 mg/kg b wt) was administered orally once a day for 30 days before ulcer induction with absolute ethanol (1 ml/ 200 g b wt). Pretreatment with HAEPD and Omeprazole inhibited the ulceration damage of absolute ethanol by 68% and 58.51%respectively.Our results also showed that gastric ulcer index was significantly reduced in rats pretreated with HAEPD as compared with ethanol-treated controls. Moreover, in rats pretreated with HAEPD, there is significant reduction of TBARS content in gastric mucosa was found as compared to those rats treated with ethanol alone. In contrast, SOD, GR, GSH-Px activities were significantly increased in gastric mucosa of HAEPD pretreated rats with respect to those treated with ethanol alone. HAEPD and Omeprazole showed decreased MPO activity when compared to ethanol treated rats as a result of gastric injuries. Hence, our results demonstrates that HAEPD pretreatment exerts gastroprotective effects in ethanol-induced gastric ulcers in rats as evidenced by stimulation of antioxidant enzymes such as SOD, CAT, GR and GSH-Px which are scavengers of ROS and therefore prevents the gastric damage induced by them.

Nadhiya K.,Research Scholar | Vijayalakshmi K.,Bharathi Womens College Autonomous
International Journal of Pharmaceutical Sciences Review and Research | Year: 2015

The present study is deliberate on the isolation and purification of ethanolic extract of Benincasa hispida fruit by column chromatography and the presence of β-sitosterol in active fraction IV is to be analysed by HPTLC method. Ethanolic extract of Benincasa hispida fruit extract was subjected to column chromatography followed by HPTLC analysis using β-Sitosterol as a standard. Similar fractions with the same Rf values were pooled. The pooled fractions were subjected to quantification of phenols, flavonoids, terpenoids and tannins. Fraction containing high amount of these secondary metabolites were subjected to HPTLC analysis using β-sitosterol, as a marker. The column chromatography analysis resulted in 84 fractions. When the fractions were subjected to TLC analysis Rf value were obtained for each fractions. According to the Rf value fractions were pooled into five fractions. Ethanolic extract of Benincasa hispida fraction IV showed the presence of different bands with different Rf values ranging from 0.04 to 0.83. Secondary metabolites in all fractions were determined. Total Phenols found in all fractions were range from 124.16 ± 4.07 to 137.7 ± 3.89, total flavonoids range from 16.85 ± 0.75 to 37.95 ± 1.00. Total tannins range from 6.3 ± 0.35 to 37.31 ± 0.36 and total terpenoids range from 4.25 ± 0.32 to 16.38 ± 0.30. β-sitosterol which is identified in fraction IV of ethanolic extract of Benincasa hispida fraction has many medicinal properties. β-sitosterol is type of phytosterol. So it can be used for the formulation of drugs for treatment of diseases like inflammatory, Hypercholesterolemia and cancer. © Global Research Online. All rights reserved.

Vijayalakshmi K.,Bharathi Womens College Autonomous | Nadhiya K.,Bharathi Womens College Autonomous | Haripriya D.,Bharathi Womens College Autonomous | Ranjani R.,Bharathi Womens College Autonomous
International Journal of Pharmacognosy and Phytochemical Research | Year: 2014

Bauhinia variegata and Garcinia cambogia are traditionally used for treating different diseases. The present study was focused on in silico analysis to elucidate the inhibitory activity of retinol binding protein 4 (RBP4) using compounds from GC-MS analysis of Bauhinia variegata and Garcinia cambogia. The present study was carried out to determine the possible chemical components from Bauhinia variegata and Garcinia cambogia by GC-MS analysis. These compounds were docked with Retinol binding protein 4. These chemical components having anti-lipidemic activity were subjected to in silico analysis. This study evaluates the inhibitory activity of compounds with Retinol binding protein 4 using Argus lab software. Compounds like Octadecanoic acid, Tertradecanoic acid, Hexadecanoic acid and Trans-Geranylgeraniol of Garcinia cambogia and compounds like phytol and Hexadecanoic acid of Bauhinia variegata were docked with RBP4. GC-MS analysis reveal 31 compounds from Garcinia cambogia while, Bauhinia variegata exhibit 9 compounds. In silico analysis showed that Tetra decanoic acid has interaction energy -9.08kcal/mol, followed by Hexadecanoic acid (-9.64Kcal/mol) and then Octadecanoic acid has -9.88Kcal/mol. Trans-geranylgeraniol showed -16.45Kcal/mol. But there was no interaction with protein and ligands of Bauhinia variegata like Hexadecanoic acid and Phytol was studied for insilico analysis. Phytol has -3.78Kcal/mol of interaction energy similarly hexadecanoic acid has -9.88Kcal/mol. But phytol did not show any hydrogen bond between the protein and ligand but Hexadecanoic acid shows hydrogen bond. While comparing these compounds Octadecanoic acid, hexadecanoic acid and tetradecanoic acid shows better interaction with RBP4 compared to Phytol and Trans-geranylgeraniol. Molecular docking analysis proved that secondary metabolites of Garcinia cambogia had better inhibitory activity against RBP4 than Bauhinia variegate and so these compounds from Garcinia cambogia may act as better drug models for obesity. © 2014, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved.

Nadhiya K.,Bharathi Womens College Autonomous | Vijayalakshmi K.,Bharathi Womens College Autonomous | Gaddam Aadinath Reddy G.,Siddha central research institute
International Journal of Pharmaceutical and Clinical Research | Year: 2016

Aim: The study was designed to evaluate the efficacy of ethanolic extract of Benincasa hispida (EEBH) and its active fraction (AFBH) on high fat diet fed rats. Methods: Male wistar rats (200±10g) were divided into five groups of 6 rats. Group I (Normal control), Group II (High fat diet(HFD), Group III (HFD+300mg/kg ethanolic fruit extract), Group IV (HFD+100mg/kg active fraction from ethanolic fruit extract) and Group V (HFD+25mg/kg Orlistat (drug control). After induction of High fat diet, Physical parameters such as body weight, organ weight, fat pad weight, anthropometric parameters were measured. Biochemical parameters such as serum lipid profile, Glucose, insulin, Homeostatic insulin resistance (HOMOIR), Free fatty acids, Phospholipids, homocysteine, apolipoprotein-B(Apo-B), leptin and adiponectin levels were analysed. Enzyme parameters such as Aspartate transaminase (AST), Alanine transaminase (ALT), Alkaline phosphatase (ALP), Lactate dehydrogenase (LDH), Creatinine kinase (CK) and lipase were analysed. Histopathological changes in the adipose tissue were identified using haemotoxylin eosin stain and similarly histopathological changes in the liver were identified using haemotoxylin eosin stain and oil red O staining. Results: Active fraction of Benincasa hispida showed better result in reducing lipid levels such as cholesterol and Triglycerides (TG), Low density lipoprotein (LDL), free fatty acids, Phospholipids, Insulin, HOMO-IR, leptin and enzyme levels. Homocysteine and apolipoprotein B (Apo-B) was an important cardioprotective marker. These parameters also were reduced in AFBH and EEBH treated groups, EEBH and AFBH increases HDL and adiponectin levels, when compared with HFD fed groups. Antilipidemic activity was observed with AFBH. © 2016, International Journal of Pharmaceutical and Clinical Research. All rights reserved.

Megala J.,Bharathiar University | Geetha A.,Bharathi Womens College Autonomous
Journal of Ethnopharmacology | Year: 2012

Ethnopharmacological relevance: The ethnopharmacological importance of Pithecellobium dulce is evidenced by its traditional use for gastric complications. The aim of the study is to evaluate the gastroprotective activity and the mechanism of action of hydroalcoholic fruit extract of P. dulce (HAEPD) in rats by using chemical and stress induced ulcer models. Materials and methods: Gastric ulcer was induced by administering alcohol (or) acetylsalicylic acid (or) hypothermic restraint stress to rats pretreated with HAEPD (200 mg/kg b wt for 30 day). Volume of gastric fluid, pH, acidity, activities of pepsin, H +, K +-ATPase, myeloperoxidase, mucin content, nucleic acids, glycoproteins and prostaglandin E 2 (PGE 2) levels were assessed in gastric tissues. Results: Ulcer score was significantly minimized in HAEPD administered animals. pH and acidity of gastric fluid were significantly minimized and the mucin, PGE 2 levels were significantly maintained in drug pre administered animals. The activities of H +, K +- ATPase and myeloperoxidase were found to be significantly elevated in ulcer control animals and found to be decreased in drug pretreated animals. The cell proliferation was found to be enhanced in drug received animals. The total protein bound carbohydrate to total protein ratio was found to be significantly maintained by HAEPD. The effects were found to be comparable with that of standard drug omeprazole. Conclusion: It is concluded that HAEPD possess a potent antiulcer activity probably by acting as cytoprotective and antiacid secretory agent. © 2012 Elsevier Ireland Ltd.

Megala J.,Vels University | Geetha A.,Bharathi Womens College Autonomous
Food Chemistry | Year: 2010

The free radical-scavenging property and the inhibitory action on H+, K+-ATPase activity of aqueous (AEPD) and hydroalcoholic (HAEPD) fruit extracts of Pithecellobium dulce (PD) were screened in various in vitro models. HAEPD showed the greatest free radical-scavenging activity in all the experimental models. Further, HAEPD has shown a potent H+, K+-ATPase inhibitory activity and comparable to that of Omeprazole, the antiulcer drug. HPLC chromatogram of HAEPD was found to contain rich quantity of phenolic compounds and revealed the presence of flavonoids - quercitrin, rutin, kaempferol, naringin, daidzein. Thus HAEPD was found to possess a good antioxidant capacity when compared to AEPD. The study indicates that PD can be screened for antiulcer activity, since overexpression of H+, K+-ATPase results in gastric ulceration. So the free radical-scavenging and antiacid secretory activities of PD may be claimed for its traditional consumption for gastric complications. © 2010 Elsevier Ltd. All rights reserved.

Janaki K.,Bharathi Womens College Autonomous | Radhakrishnan N.,Anna University
International Review on Computers and Software | Year: 2013

It offers, indeed, immense challenges to assess clearly the exact variance of the optimal solutions with the recent remedies, when we set out to study the locations of modern hospitals in the context of the perplexing state of affairs and the intricate decisive factors prevalent in the current scenario. In order to do so, we first identify the main classes of criteria used in location planning. In the existing hospital location analysis technique a non linear mathematical model is developed to find the highly sensitive roads. But this method has such a drawback that the obtained optimal weights are not generally distributed. To overcome this, a new expert system developed for hospital location analysis with optimization method is presented. In our proposed technique, a new expert system is developed based on the Radial Basis Function (RBF). In the RBF the weights are optimized by using the well renowned optimization technique Adaptive Artificial Bee Colony (AABC) Algorithm. After that, the different length pattern values are mined and locations are computed by using the pattern mining and clustering techniques. Thus our proposed technique performance is analyzed by using the hospital location dataset and compared with the exiting hospital locations analysis techniques and optimizations methods PSO and Conventional ABC. © 2013 Praise Worthy Prize S.r.l. - All rights reserved.

Megala J.,Bharathiar University | Geetha A.,Bharathi Womens College Autonomous
Natural Product Research | Year: 2012

This study was carried out to evaluate the acute and sub-acute toxicity profile of the hydroalcoholic fruit extract (HAEPD) of Pithecellobium dulce (Leguminosae). Albino rats were treated orally with 100, 200 and 500mg kg-1 bodyweight (BW) of HAEPD for 90 days to assess its sub-acute toxicity. HAEPD at single doses of 100, 500, 1000, 2000 and 4000mg kg -1 BW was also administered to rats to assess its acute toxicity. The rats were observed for physical discomfort, BW change and feeding habits. Pithecellobium dulce did not cause any abnormal changes in haematological or biochemical parameters. Pathologically, no gross abnormality in the tissue architecture was observed. The LD 50 was found to be 3916mg kg -1 BW and potential effective doses for efficacy studies are 100 and 300mg kg -1 BW as the minimum and maximum doses, respectively. It is concluded that HAEPD can be used safely for experimental and clinical trials. © 2012 Taylor & Francis.

Chandramohan Y.,Bharathi Womens College Autonomous | Parameswari C.S.,Bharathi Womens College Autonomous
International Journal of Pharma and Bio Sciences | Year: 2013

The therapeutic effecicacy of a flavonoid Naringin (NG) against Cyclosporine A (CsA) induced nephrotoxicity was investigated in this study. Nephrotoxicity was induced in male albino wistar rats by injecting 25mg/kg body weight of CsA for a period of 21 days. CsA induced rats were also cotreated with 40mg of Naringin (NG/kg body weight) orally. After the experimental period the serum levels of urea, uric acid and creatinine were found to be elevated, followed by the decrease in creatinine clearance, whereas the levels of ALP, ACP were increased in serum of CsA induced rats. A significant decrease in LDH levels were observed in CsA induced animals compared to control. Immunohistochemical staining of inducible nitric oxide synthase (iNOS) expression was studied and NG co treatment significantly decreased the expression of iNOS in CsA challenged rats. This data was further evidenced by western blotting expression pattern of iNOS in experimental rats.

Surendran S.,Bharathiar University | Krishnamoorthy V.,Bharathi Womens College Autonomous
International Journal of Pharma and Bio Sciences | Year: 2014

The present study evaluated the effect of ethanolic extracts of Cyamopsis tetragonolobus rhizomes and Cyperus rotundus pods on the biochemical parameters of diabetic cataract induced Wistar albino rats. Healthy rats were grouped into 4: Group I - Normal, Group II - Diabetic cataract induced animals (STZ-induced diabetic rats which developed cataract), Group III - Cyamopsis tetragonolobus extract co-treated animals and Group IV - Cyperus rotundus extract co-treated animals (Treatment for 19 weeks after STZ induction). Body weight and organ weight of cataract animals decreased while plant treatment prevented this loss. Increase in values of glucose, glycated haemoglobin, insulin, creatinine, urea and uric acid levels were observed in diabetic cataract induced rats, while plant co-treated groups restored these levels. The changes in lipid and protein profile in Group II rats were prevented by the plant treatment. SGOT, SGPT, ALP and LDH increased in Group II but plant treatment retained enzymatic activities. The results signify the plant's protective nature.

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