Bharat Institute of Pharmacy

Ibrahimpatnam, India

Bharat Institute of Pharmacy

Ibrahimpatnam, India
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Elphine Prabahar A.,Chalapathi Institute of Pharmaceutical Science | Vijayaraj Kumar P.,Bharat Institute of Pharmacy
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2010

Two simple, rapid and sensitive extractive spectrophotometry methods have been developed for the assay of cinitapride in pure and pharmaceutical formulations. These methods are based on the formation of chloroform soluble ion-association complexes of cinitapride with Bromocresol Green (BCG) and with Bromothymol Blue (BTB) in potassium hydrogen phthalate buffer pH-4 with absorption maximum at 414 nm and 416 nm for BCG and BTB, respectively. Reaction conditions were optimized to obtain the maximum colour intensity. The absorbance was found to increase linearly with increase in concentration of cinitapride, which was corroborated by the calculated correlation coefficient values (0.9999 and 0.9998). The systems obeyed Beer's law in the range of 5-40 and 2-10 μg/mL for BCG and BTB, respectively. Various analytical parameters have been evaluated and the results have been validated by statistical data. No interference was observed from common excipients present in tablets.

Vijayaraj Kumar P.,Bharat Institute of Pharmacy
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2011

Two simple and sensitive spectrophotometry methods (A and B) for the determination of Pramipexole Dihydrochloride in pure and pharmaceutical formulations are described. In method A, distilled water was used as solvent and shows absorbance maximum at 262 nm. In method B, methanol was used as solvent and shows absorbance maximum at 263 nm. In method A linearity was found to be in the range of 10 - 50 μg/ml and for method B linearity was 5 - 40 μg/ml; for method A (Y=0.02 X-0.003; r2=0.9996) and for method B (Y=0.0291 X+0.0073; r2=0.9987), respectively. The proposed methods were successfully applied for the determination of Pramipexole in pharmaceutical formulations.

Samala A.,Bharat Institute of Pharmacy | Sasikala M.,Bharat Institute of Pharmacy | Chatlapelli K.,Bharat Institute of Pharmacy
Journal of Chemical and Pharmaceutical Research | Year: 2014

A simple, accurate, rapid and isocratic reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed for estimation of Ropinirole hydrochloride in tablet dosage forms.Chromatographic separation was operated with 0.05mol glacial acetic acid (pH 3): acetonitrile (50:50v/v) at a flow rate of 1ml/min on hypersil C18 (BDS) column, using 250nm as detection wavelength. The retention time for Ropinirole hydrochloride was found to be 4.037min. The method was linear over the concentration range of 4-12μg/ml and coefficient correlation was found to be 0.997. The method showed good recoveries (99.0-99.89%) and its intraday and interday precision were found to be 0.39 and 0.479 respectively. For its sensitivity and reliability, the proposed method can be used for quality control assay of Ropinirole hydrochloride.

Vijayalakshmi P.,Sree Dattha Institute of Pharmacy | Venkateswara Rao J.,Bharat Institute of Pharmacy
International Journal of Drug Delivery | Year: 2014

The aim of the present investigation was to development of immediate release liquid coated pellets of poorly soluble drugs Amlodipine besylate (AMD) and Nebivolol HCl (NBV) by novel liquid layering technology to enhance solubility and bioavailability with HPC-EF as hydrophilic polymer and PVP K 30 as binder. A 32 full factorial design was employed to optimize the formulation of pellets. In order to optimize formulations, two polymers HPC-EF and PVP K 30 as factors and amount of polymers (three different concentrations), were taken as independent variables. All the formulations were evaluated for particle size, friability, moisture content, drug content, in vitro dissolution studies and in vivo bioavailability studies. All the formulations were found uniform with respect to all evaluation parameters. The optimized formulation (F5) showed highest % of drug release 99.59 by the end of 8 min for AMD and 99.21 % of drug release for NBV, when compared with the marketed product (NEBISTAR-AM) the percentage of AMD and NBV was 83.91 and 82.67 respectively within 8 min, by using 4% of HPCEF and 1% of PVP K 30. SEM confirmed that F5 was spherical in shape with a smooth surface. In vivo studies indicated significant difference in the bioavailability between AMD and NBV coated pellets with pure drug. Clinical data confirmed that the optimized formulation (F5) by choosing immediate release drug coated pellet technology by liquid layering method could improve patient compliance and ensure better disease management when compared with the marketed product. © 2014, Advanced Research Journals , All Rights Reserved.

Mahto M.K.,Acharya Nagarjuna University | Likhitha Raj V.,Bharat Institute of Pharmacy | Bhaskar M.,Sri Venkateswara University | Divya R.,Gulbarga University
International Journal of Pharmaceutical Sciences Review and Research | Year: 2012

Now days, Influenza is one of the highly potent contagious pathogen spreading and threatening all over the world by causing dreadful respiratory diseases like Swine flu for which enzyme Neuraminidase (NA) is the major drug target. Neuraminidase, otherwise called as sialidases, exists as a mushroom shape projection on the surface; catalyze the hydrolysis of terminal sialic acid residues from the newly formed virions and from the host cell receptors. Reports of the recent studies reveal that enzyme neuraminidase is resistant to drugs like Oseltamivir. In the present study, we have used commercial computational tools like Accelry's Discovery Studio 2.5 to identify the novel analogs that established better binding than the Zanamivir. From the docking studies, we found that substitution of hydroxyl group with methyl is having better dock score and higher interaction energy than Zanamivir.

Sharma A.,Bharat Institute of Pharmacy | Singh R.,Maharishi Markandeshwar University
Mini-Reviews in Organic Chemistry | Year: 2015

Imidazole, an important azole heterocycle, having biological and medicinal properties prompted various research groups to develop different synthetic routes to this heterocycle. This review is an attempt to focus on the various synthetic routes involving conventional and new greener approaches for the synthesis of trisubstituted imidazoles. © 2015 Bentham Science Publishers.

Kumar P.,Hetro labs Ltd | Singh R.,Maharishi Markandeshwar University | Sharma A.,Bharat Institute of Pharmacy
Current Computer-Aided Drug Design | Year: 2015

Candida albicans is one of the most important causes of life-threating fungal infections. Lanosterol 14α-demethylase (Cytochrome P450DM) is the target enzyme of azole antifungal agents. The study involved selection and modeling of the target enzyme followed by refinement of the model using molecular dynamic simulation. The modeled structure of enzyme was validated using Ramachandran plot and Sequence determination technique. A series of chlorosubstituted imidazole analogues were evaluated for Cytochrome P450 inhibitory activity using molecular docking studies. The imidazole analogues were prepared using Chem sketch and molecular docking was performed using Molergo Virtual Docker program. The docking study indicated that all the imidazole analogues (AN1-AN45) and standard drugs i.e., Ketoconazole, Clotrimazole and Miconazole have interaction with protein residue of 14α-demethylase, Heme cofactor and the water molecules present in the active site. © 2015 Bentham Science Publishers.

Hemanth kumar G.,Bharat institute of pharmacy
International Journal of Research in Pharmaceutical Sciences | Year: 2012

Today three fourth of the drugs manufactured are taken orally and are not found to be effective as desired. When compared to oral route of administration, transdermal route has numerous advantages over the more traditional drug delivery systems. Drug delivery through the skin to attain systemic circulation is known as transdermal drug delivery system (TDD).TDDS are the dosage forms which involves penetration of the drug substances through the outermost layer of skin that is stratum corneum to show its therapeutic effect, where major part of drug is trans-ported into systemic circulation. A Transdermal Patch is an adhesive patch that has a coating of medicine (drug) that is placed on the skin to deliver specific dose (drug) into the blood over a period of time. This review describes about recent advancements in TDD enhancement techniques, skin morphology & mechanism of penetration, components of Transdermal patches, types of patches, and its physicochemical methods of evaluation is de-scribed. © JK Welfare & Pharmascope Foundation.

Thangabalan B.,Southern Institute of Medical science | Vijayaraj Kumar P.,Bharat Institute of Pharmacy
Asian Journal of Chemistry | Year: 2011

A sensitive and rapid extractive spectrophotometeric method has been developed for the assay of acipimox in pharmaceutical formulation. The method is based on the formation of a chloroform soluble ion-pair complex between acipimox and methylene blue in a basic medium. The complex shows absorption maximum at 662 nm and the system obeys Beer's law in the concentration range of 2-10 μg/mL. The results obtained by the proposed method were validated statistically and by recovery studies.

Thangabalan B.,Southern Institute of Medical science | Kumar P.V.,Bharat Institute of Pharmacy
Asian Journal of Chemistry | Year: 2011

A reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for the estimation of acipimox in bulk drug and pharmaceutical dosage forms. The quantification was carried out on Luna C18 column in isocratic mode, with mobile phase consisting of 0.1 % v/v phosphoric acid in water and acetonitrile in the ratio of 95:5 [v/v]. The mobile phase was pumped at a rate of 1.0 mL/min and the detection was carried out at 229 nm and the linearity was found to be in the range of 20-300 μg/mL. The regression equation was found to be Y = 33672x + 6367.2 with correlation coefficient [r2] of 0.9998. The percentage recovery values were found to be in the range of 99.96-100.07 %. Validation of the proposed method has also been done.

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