BGR Bundesansaltfur Geowissenschaften und Rohstoffe

Hannover, Germany

BGR Bundesansaltfur Geowissenschaften und Rohstoffe

Hannover, Germany
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Juarez E.,Instituto Nacional Of Enfermedades Respiratorias | Ronquillo de Jesus E.,Metropolitan Autonomous University | Nieto-Camacho A.,National Autonomous University of Mexico | Kaufhold S.,BGR Bundesansaltfur Geowissenschaften und Rohstoffe | And 4 more authors.
Applied Clay Science | Year: 2016

Sepiolite and palygorskite have shown beneficial health effects but understanding human cell-clay interactions has yet to become unveiled. This paper reports on the effects of sepiolite (Vallecas, Spain) and palygorskite (Torrejon El Rubio, Spain) on the infiltration of human blood leukocytes to an infiltration site. Quantification of human blood leukocyte cells under pro- and anti-inflammatory conditions was conducted, and cells visualized in an Axioscope (Carl Zeiss; Oberkochen, Germany). Images were recorded with an Axiocam Mrm monochromatic camera and ZEN Pro software (Carl Zeiss). The distribution of human blood leukocyte cells at the inflammation site varied before and after adding the clay. The relative proportion of PMN-to-monocytes(MN) (PMN/MN) exposed to the inflammatory activity by 12-O-tetradecanoylphorbol-13-acetate (TPA) changed in the presence of sepiolite (TPA + sepiolite) or palygorskite (TPA + palygorskite) either after 4 or 24. h, namely, 0.60, 2.5, and 2.33; and 4.33, 1.53, and 2.8, respectively. PMN/MN values compared in the presence of TPA or TPA and palygorskite, however decreased sharply in the presence of TPA and sepiolite. Proposedly, decreases in PMN/MN values caused by adding sepiolite may alter PMN and MN immunological functions, by lessening the destruction extent of invasive bacteria via phagocytosis and the conversion of MN to macrophages. Proposedly, limiting a conversion of MN to macrophages impedes resolving inflammation because of an incomplete digestion of aged cells. Evidently, shifting from pro- to anti-inflammatory conditions due to the addition of the clay altered the mechanism of infiltration of different leukocyte cells to an inflammation site. Finally, the presence of few macrophages at the inflammation site was attributed to resolution of inflammation, whereby macrophages participated in anti-inflammatory mechanisms leading to the return to homeostasis in tissues. © 2016 Elsevier B.V.


Cervini-Silva J.,Metropolitan Autonomous University | Cervini-Silva J.,Lawrence Berkeley National Laboratory | Cervini-Silva J.,NASA | Camacho A.N.,National Autonomous University of Mexico | And 12 more authors.
Applied Clay Science | Year: 2016

This manuscript reports on the effects of natural Fe-halloysite matrices on infiltration and migration of neutrophils (polymorphonuclear (PMN) leukocytes), which, after the skin, constitute the primary protection of organisms against pathogens. Speciation of mineral Fe was quantified before and after treatment with citrate-bicarbonate-dithionite (CBD). Infiltration and migration of inflammatory and immune effector cells, and cell viability were quantified using the 12- O-tetradecanoylphorbol-13-acetate (TPA) and myeloperoxidase (MPO) enzymatic activity methods, and the Griess assay. Halloysite was collected ~. 2 km from Opotiki, Bay of Plenty, New Zealand. HRSEM images confirmed typical morphological features proper of spheroidal Hal (S-Hal). Mössbauer spectroscopy of S-Hal confirmed the presence of Fe, octahedrally coordinated in the form of substituted Fe(III), magnetically ordered goethite or ferrihydrite. HRTEM images showed the presence of small-size domains of Fe (~. 3-nm) predominantly in the form of ferrihydrite. EPR analyses of S-Hal (0-5000. ppm) before and after reacting with desferrioxamine-B confirmed the fast release of Fe from the nanodomains of ferrihydrite. Early inhibition of edema by S-Hal doubled that by CBD treated Hal (t-S-Hal), explained because labile Fe (2- L-ferrihydrite) enhanced the 4-h anti-inflammatory response. On the other hand, prolonged inhibition of edema by S-Hal and t-S-Hal compared, consistent with the release of Fe from the Hal structure. The presence of S-Hal or t-S-Hal related to the inhibition of MPO content. After 4 h, the inhibition of MPO content by S-Hal or t-S-Hal compared to that by commercial indomethacin (ca. 80%). S-Hal or t-S-Hal showed high inhibition of MPO contents shortly after exposure, but decreased sharply afterwards. On the other hand, tubular Hal (T-Hal) caused an increasing inhibition of MPO with time, explained because clay structure restricted the kinetics and mechanism of MPO inhibition. Evidenced showed that the release of mineral Fe related to infiltration and migration of inflammatory and immune effector cells, expanding the knowledge that metal ions affect inflammatory responses. Finally, dose-response experiments confirmed that the inhibition of edema and cell viability were surface-mediated. Natural clay reservoirs are complex in composition, therefore identifying the molecular mechanism(s) regulating cell migration and infiltration becomes necessary prior to recommending their use for healing purposes. © 2015.


Cervini-Silva J.,Metropolitan Autonomous University | Cervini-Silva J.,Lawrence Berkeley National Laboratory | Antonio-Nieto-Camacho,National Autonomous University of Mexico | Gomez-Vidales V.,National Autonomous University of Mexico | And 7 more authors.
Journal of Hazardous Materials | Year: 2014

This paper reports that the main component of respirable volcanic ash, allophane, induces lipid peroxidation (LP), the oxidative degradation of lipids in cell membranes, and cytotoxicity in murin monocyle/macrophage cells. Naturally-occurring allophane collected from New Zealand, Japan, and Ecuador was studied. The quantification of LP was conducted using the Thiobarbituric Acid Reactive Substances (TBARS) assay. The cytotoxic effect was determined by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide colorimetric assay. Electron-Paramagnetic Resonance (EPR) determinations of naturally-occurring allophane confirmed the incorporation in the structure and clustering of structural Fe3+, and nucleation and growth of small-sized Fe (oxyhydr)oxide or gibbsite. LP induced by allophane varied with time, and solid concentration and composition, reaching 6.7±0.2nmolTBARSmgprot-1. LP was surface controlled but not restricted by structural or surface-bound Fe3+, because redox processes induced by soluble components other than perferryl iron. The reactivity of Fe3+ soluble species stemming from surface-bound Fe3+ or small-sized Fe3+ refractory minerals in allophane surpassed that of structural Fe3+ located in tetrahedral or octahedral sites of phyllosilicates or bulk iron oxides. Desferrioxamine B mesylate salt (DFOB) or ethylenediaminetetraacetic acid (EDTA) inhibited LP. EDTA acted as a more effective inhibitor, explained by multiple electron transfer pathways. Registered cell-viability values were as low as 68.5±6.7%. © 2014 Elsevier B.V.


Cervini-Silva J.,Metropolitan Autonomous University | Cervini-Silva J.,Lawrence Berkeley National Laboratory | Cervini-Silva J.,NASA | Ramirez-Apan M.T.,National Autonomous University of Mexico | And 4 more authors.
Chemosphere | Year: 2016

Bentonites, naturally occurring clays, are produced industrially because of their adsorbent capacity but little is known about their effects on human health. This manuscript reports on the effect of bentonites on cell growth behaviour. Bentonites collected from India (Bent-India), Hungary (Bent-Hungary), Argentina (Bent-Argentina), and Indonesia (Bent-Indonesia) were studied. All four bentonites were screened in-vitro against two human cancer cell lines [U251 (central nervous system, glioblastoma) and SKLU-1 (lung adenocarcinoma)] supplied by the National Cancer Institute (USA). Bentonites induced growth inhibition in the presence of U251 cells, and growth increment in the presence of SKLU-1 cells, showing that interactions between bentonite and cell surfaces were highly specific. The proliferation response for U251 cells was explained because clay surfaces controlled the levels of metabolic growth components, thereby inhibiting the development of high-grade gliomas, particularly primary glioblastomas. On the other hand, the proliferation response for SKLU-1 was explained by an exacerbated growth favoured by swelling, and concomitant accumulation of solutes, and their hydration and transformation via clay-surface mediated reactions. © 2016 Elsevier Ltd.


Cervini-Silva J.,Metropolitan Autonomous University | Cervini-Silva J.,Lawrence Berkeley National Laboratory | Cervini-Silva J.,NASA | Antonio-Nieto-Camacho,National University of Costa Rica | And 3 more authors.
Applied Clay Science | Year: 2015

Bentonites, naturally occurring clays, are produced industrially because of their adsorbent capacity but little is known about their effects on human health. This manuscript reports on the anti-inflammatory activity of bentonites. Bentonites collected from India (Bent-India), Hungary (Bent-Hungary), Argentina (Bent-Argentina), and Indonesia (Bent-Indonesia) were studied. All four bentonites were tested for anti-inflammatory activity using the mouse ear edema and the 12-O-tetradecanoylphorbol-13-acetate (TPA) method. Bentonites inhibited edema after 4. h (EI %), regardless of composition. A direct comparison between EI and surface area values showed that Bent-Argentina was four times more active than the other bentonites, with infiltration preferred in the former case. We attributed the inhibition of edema by bentonites to clay swelling, causing physical occlusion thereby limiting the movement of leukocyte cells towards the inflammation site. Expandability over changes in mineral composition underpinned inhibition of edema by bentonites, while evidence lacked to support chemical-transfer mechanism(s). © 2015 Elsevier B.V.


Cervini-Silva J.,Metropolitan Autonomous University | Cervini-Silva J.,Lawrence Berkeley National Laboratory | Nieto-Camacho A.,National Autonomous University of Mexico | Gomez-Vidales V.,National Autonomous University of Mexico | And 2 more authors.
Applied Clay Science | Year: 2015

This paper presents evidence of the novel anti-inflammatory properties of natural allophane collected from New Zealand, Japan, and Ecuador. Allophanes were assessed by (i) the mouse-ear edema method using 12-O-tetradecanoylphorbol-13-acetate (TPA) as inflammatory agent; and (ii) the myeloperoxidase (MPO) enzymatic-activity method. After 4h, applying 1mgear-1 allophane conveyed edema inhibition (EI; p≤0.01) in up to 39%, while MPO content inhibition (CI) values surpassed 60%. Pearson's correlation analysis between EI and MPO data showed that edema was mediated by the migration of neutrophils at t=4h (p<0.05), but not at t=24h. The lack of variation in cellular migration with time was explained because of a reaction of zero-order kinetics. EPR spectra for allophanes showing higher anti-inflammatory activity denoted a broad signal centered at g=2, and an intense spin-spin interaction, typical of a low-spin, octahedral Fe3+ environment (S=1/2); and overlapping signals typical for Ni, with octahedral coordination, explained either by oxidation states +1 (Ni1+), +3 (Ni3+), or bulk Ni2+ ions. © 2014.


PubMed | BGR Bundesansaltfur Geowissenschaften und Rohstoffe, Lawrence Berkeley National Laboratory, Direccion de Investigacion y Posgrado and National Autonomous University of Mexico
Type: | Journal: Chemosphere | Year: 2016

Bentonites, naturally occurring clays, are produced industrially because of their adsorbent capacity but little is known about their effects on human health. This manuscript reports on the effect of bentonites on cell growth behaviour. Bentonites collected from India (Bent-India), Hungary (Bent-Hungary), Argentina (Bent-Argentina), and Indonesia (Bent-Indonesia) were studied. All four bentonites were screened in-vitro against two human cancer cell lines [U251 (central nervous system, glioblastoma) and SKLU-1 (lung adenocarcinoma)] supplied by the National Cancer Institute (USA). Bentonites induced growth inhibition in the presence of U251 cells, and growth increment in the presence of SKLU-1 cells, showing that interactions between bentonite and cell surfaces were highly specific. The proliferation response for U251 cells was explained because clay surfaces controlled the levels of metabolic growth components, thereby inhibiting the development of high-grade gliomas, particularly primary glioblastomas. On the other hand, the proliferation response for SKLU-1 was explained by an exacerbated growth favoured by swelling, and concomitant accumulation of solutes, and their hydration and transformation via clay-surface mediated reactions.

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