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Ma W.-H.,Bethune International Peace Hospital of Chinese PLA | Zhang X.-M.,Hebei National Defence Construction Hospital | Shi S.-S.,Chinese PLA General Hospital | Li Y.-F.,Chinese PLA General Hospital
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010

BACKGROUND: In vitro research of bone cells were difficult for result analysis due to various factors. The separated bone cells could obtain single cell line. Therefore, the single cell line was used to observe direct effect of various materials or methods and avoid some interfering factors. OBJECTIVE: To investigate a feasible method to mass-produce, culture, and reserve normal osteoblast of people. METHODS: Diaphysis of extremities from normal fetus aged four months were harvested to digest in collagenase and pancreatinto generate single cell suspensions, the cells and tissue segments were cultured for establishing normal osteoblast system of people. Morphology and histology were observed; type collagen produce was measured using immunohistochemistry; cells were reserved using liquid nitrogen frozen method; cells in good logarithmic growth phase were collected for preparation of genetics-chromosome. RESULTS AND CONCLUSION: Osteoblast was fusiformed-shaped and had plentiful processes. Nucleus was orbicular-ovateand leaning to lateral side. Soma was large, and plasma was abundant. Alkaline phosphatase staining suggested that a great number of gray-black particles were observed in plasma, and some region was darkly stained. Quantitative analysis demonstrated that levels of alkaline phosphatase and osteocalcin in the plasma were significantly higher than fibroblast; immunohistochemical staining suggested that the type collagen was mainly produced. Chromosome analysis indicated that there were 23 pairs of chromosomes, and abnormal chromosome was not detected, suggesting that the obtained samples were normal human cells which were still normal following various cultures. Fluorochrome-tetracycline staining demonstrated that the cultured cells in this study had the osteogenesis ability. In this study, a normal osteoblast system of people was established and could be reserved with liquid nitrogen for a long time. The cells had stable biological characteristics following both various passages and frozen reservation. Source


Li J.,Hebei Medical University | Wu H.,Bethune International Peace Hospital of Chinese PLA | Xue G.,Bethune International Peace Hospital of Chinese PLA | Wang P.,Hebei General Hospital | Hou Y.,Hebei Medical University
Basic and Clinical Pharmacology and Toxicology | Year: 2013

Numerous studies in rodents have indicated that exposure to ketamine during the period when synaptogenesis is highly active induces neurodegeneration. Thus, there is a growing need to develop strategies to prevent ketamine-induced brain injury in the developing brain. Oestradiol is a neuroactive steroid that prevents neuronal cell death in different experimental models by activating cell survival signals and inhibiting apoptotic signals. The main goal of this study was to investigate the neuroprotective effects of 17β-oestradiol against ketamine-induced apoptotic neurodegeneration in primary-cultured cortical neurons. The data revealed that 17β-oestradiol (0.1 μM) in combination with ketamine (100 μM) increased cell viability in the MTT assay and reduced the number of apoptotic cells detected by TUNEL and Hoechst 33258 staining. To elucidate a possible mechanism by which 17β-oestradiol exerts its neuroprotective effect, we investigated the PI3K pathway using an inhibitor of PI3K, LY294002. The protective effects of 17β-oestradiol were abrogated by LY294002. Furthermore, we found that 17β-oestradiol not only induced phosphorylation of the PI3K substrate Akt, but also increased the expression of Bcl-2, which down-regulated ketamine-induced caspase-3 activity and inhibited neuronal apoptosis. These data demonstrate that 17β-oestradiol exerts a neuroprotective effect against ketamine-induced neuronal apoptosis by activating the PI3K/Akt/Bcl-2 signalling pathway. Therefore, 17β-oestradiol appears to be a promising agent in preventing or reversing ketamine's toxic effects on neurons at an early developmental stage. © 2013 Nordic Pharmacological Society. Published by John Wiley & Sons Ltd. Source


Zhao Q.-T.,Hebei General Hospital | Yang Z.-X.,Hebei General Hospital | Yang L.,Bethune International Peace Hospital of Chinese PLA | Xing D.,Hebei General Hospital | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

Aim and Backgrounds: The accurate diagnosis of lung carcinoma patients with bone metastases is crucial for therapy and the prevention of complications. We performed a systematic review and meta-analysis to evaluate the diagnostic value of serum bone-specific alkaline phosphatase (BALP) in lung carcinoma patients with bone metastases. Methods: Such databases as PubMed, Embase, Cochrane Library, Web of Science, Ovid, BioMed Central, Biosis previews and four Chinese databases (Chinese Biomedical Literature Database-disc (CBM), Chinese National Knowledge Infrastructure (CNKI), Technology of Chongqing (VIP) and Wan Fang DATA) were retrieved on computer, and the relevant journals were also manually searched to collect the trials on BALP in diagnosis of lung carcinoma patients with bone metastases. The meta-analysis was conducted by using Meta-Disc 1.4 software. Results: A total of 8 studies were included, and there were 848 lung carcinoma patients diagnosed by gold standard, patients were divided into two groups: 419 cases with bone metastases and 429 cases without bone metastases. The meta-analysis showed that, the pooled sensitivity (SEN), specificity (SPE), positive likelihood ratio (PLR), negative likelihood ratio (NLR) and diagnostic odds ratio (DOR) was 0.48 [95% CI (0.43 to 0.53)], 0.86 [95% CI (0.82 to 0.89)], 3.14 [95% CI (2.47 to 3.99)], 0.62 [95% CI (0.56 to 0.68)], 6.66 [95% CI (4.62 to 9.60)] respectively. And the AUC of SROC was 0.78, (Q*=0.72). Conclusion: BALP has greater diagnostic value in detecting lung carcinoma patients with bone metastases. However, further large scale studies are required to confirm the predictive value. © 2015, International Journal of Clinical and Experimental Medicine. All rights reserved. Source


Wang G.,Capital Medical University | Tian J.,Capital Medical University | Zhu L.-Y.,Bethune International Peace Hospital of Chinese PLA | Yang S.,Bethune International Peace Hospital of Chinese PLA | And 7 more authors.
International Journal of Lower Extremity Wounds | Year: 2015

We aimed to investigate the characteristics of bacterial profiles and antibiotic sensitivity in diabetic foot ulcers before and after wound bed preparation. This study involved 423 diabetic patients with Wagner grades 1 to 4 foot ulcers. Secretion culture was performed before wound bed preparation. The observation endpoint was when the wound showed a tendency toward healing and a specialist determined that stopping antibiotic treatment would not affect wound healing. A second secretion culture was performed after the observation endpoint. We obtained results from both secretion cultures from 411 patients. The proportion of multi-drug-resistant (MDR) gram-positive bacteria was 22.0% and 47.8% before and after treatment, respectively; that for gram-negative bacteria was 3.5% and 19.2%, respectively (P <.05). Pretreatment antibiotic sensitivity of staphylococci and other gram-positive bacteria was 48.7% and 44.8%, respectively; the rates decreased significantly after treatment to 36.8% (P =.031) and 34.8% (P =.027), respectively. Pretreatment antibiotic sensitivity of common and nonfermenting rare gram-negative bacteria was 55.4% and 54.6%, respectively, which decreased substantially after treatment to 33.2% (P =.002) and 32.9% (P =.003), respectively. Wound healing was achieved in 92.7% of patients. Pretreatment and posttreatment C-reactive protein levels were 124.759 ± 71.58 mg/dL and 82.8 ± 53.61 mg/dL, respectively (P <.05). In conclusion, following wound bed preparation for diabetic foot ulcers, MDR bacteria numbers were increased and antibiotic sensitivity was decreased; inflammation was decreased. These findings warrant future studies for confirmation. © The Author(s) 2014. Source


Ma W.-H.,Bethune International Peace Hospital of Chinese PLA | Zhang X.-M.,National Defense Hospital of Hebei Province | Wang J.-F.,Chinese PLA General Hospital
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010

BACKGROUND: The main factors that influence revision of acetabular bone defects include evaluation of acetabular bone defects, reconstruction of acetabulum and appropriate acetabular cups used in revision. OBJECTIVE: The review from the three aspects will help to choose an appropriate cup in revision and to formulate the best revision protocols. METHODS: A computer-based online search of PubMed was performed for English articles published from January 1900 to June 2009 with the key words of acetabulum, revision, bone defect, in English and Chinese. Clinical studies published in core periodicals of the latest twenty years were reviewed. Articles regarding primary joint replacement, femoral prosthesis revision and animal experiments were excluded. RESULTS AND CONCLUSION: A total of 61 articles were analyzed, 31 were excluded, and 30 mainly published in 5 years, were included. Reconstruction of acetabular bone structure, restoration of original rotation center of hip joint are key factors for repair. Moreover, appropriate prosthesis further benefits the repair effect. Bone transplantation for bone defect and cementedless prosthesis have become favorable methods for revision of acetabular bone defects. Source

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