Peter S.,University of Tubingen |
Harter K.,University of Tubingen |
Schleifenbaum F.,University of Tubingen |
Schleifenbaum F.,Berthold Technologies GmbH and Co. KG
Methods in Molecular Biology | Year: 2014
Optical microscopy has developed as an indispensable tool for Arabidopsis cell biology. This is due to the high sensitivity, good spatial resolution, minimal invasiveness, and availability of autofluorescent proteins, which can be specifically fused to a distinct protein of interest. In this chapter, we introduce the theoretical concepts of fluorescence emission necessary to accomplish quantitative and functional cell biology using optical microscopy. The main focus lies on spectroscopic techniques, which, in addition to intensity-based studies, provide functional insight into cellular processes. © 2014 Springer Science+Business Media New York.
Berthold F.,Berthold Technologies GmbH and Co. KG |
Tarkkanen V.,503 Chemin du Pied Marin II
Luminescence | Year: 2013
This article, written by two entrepreneurs in luminescence, traces their involvement in the major part of the interconnected innovation and development of luminometers, adenosine triphosphate (ATP) bioluminescence and other technologies from the mid-1970s to 2011 that ushered in much of the field of luminometry as we know it today. Key developments leading to current commercial applications of ATP bioluminescence, luminescence immunoassay, cellular luminescence, reporter gene and other applications are described from the first tube luminometers derived from early luminescence studies using liquid scintillation counting technology to measuring bioluminescence from crude ATP and firefly tail extracts. Copyright © 2012 John Wiley & Sons, Ltd.
Klett A.,Berthold Technologies GmbH and Co. KG |
Leuschner A.,German Electron Synchrotron |
Tesch N.,German Electron Synchrotron
Radiation Measurements | Year: 2010
Many active radiation detectors exhibit severe limitations in pulsed radiation fields, which cannot be easily overcome without the design of new technology. Therefore a new area dose monitor for the measurement of pulsed and continuous neutron and gamma radiation fields was developed. The measurement of pulsed radiation is based on activation of short-lived nuclides in the detector materials or in the surroundings. The instrument comprises a thermal neutron detector in a moderator for neutron detection and a plastic scintillator for beta and gamma detection. These detectors are measuring direct radiation and also decay particles from the instable activated nuclei. The data are time-resolved acquired by a fast FADC module and the delayed decay products are identified by analyzing their timing characteristics. Measurements with pulsed and with continuous radiation are reported. © 2010 Published by Elsevier Ltd. All rights reserved.
Calmet D.,French Atomic Energy Commission |
Ameon R.,Institute for Radiological Protection and Nuclear Safety |
Beck T.,Federal office for Radiation Protection |
Bombard A.,Triskem International |
And 17 more authors.
Radiation Protection Dosimetry | Year: 2011
Radon is considered to be the main source of human exposure to natural radiation. As stated by the World Health Organization, the exposure due to the inhalation of indoor radon is much greater than the one via the ingestion of water as radon degasses from water during handling. In response to these concerns about the universal presence of radon, environmental assessment studies are regularly commissioned to assess the radon exposure of public and workers. The credibility of such studies relies on the quality and reliability of radon analysis as well as on the sample representativeness of the radiological situation. The standard-setting approach, based on consensus, seemed to lend itself to a settlement of technical aspects of potential comparison. At present, two Working Groups of the International Standardization Organization are focussing on drafting standards on radon and its decay products measurement in air and water. These standards, which aim for a set of rigorous metrology practices, will be useful for persons in charge of the initial characterisation of a site with respect to natural radioactivity as well as to those performing the routine surveillance of specific sites. © The Author 2011. Published by Oxford University Press. All rights reserved.
Mormann D.,Berthold Technologies GmbH and Co. KG |
Schmid J.,Berthold Technologies GmbH and Co. KG |
Spagnul S.,Ergolines Laboratory s.r.l.
Stahl und Eisen | Year: 2011
In recent years the dominant radiometric mould level measurement has been supplemented by electromagnetic or eddy current methods. These typically provide faster response to level changes with a higher level resolution while not being influenced by mould powder addition. Yet, eddy current instruments available on the market are limited by temperature drifts, incompatibility with electromagnetic brakes, stirrers and mould coatings. We present recent technological progress and a comparison of both the radiometric and eddy current mould level measurement methods and their impact on on-line process control.