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Hildebrand L.,Charite - Medical University of Berlin | Hildebrand L.,Berlin Brandenburg Center for Regenerative Therapies | Rossbach B.,Charite - Medical University of Berlin | Rossbach B.,Berlin Brandenburg Center for Regenerative Therapies | And 13 more authors.
Stem Cell Research | Year: 2016

Fibrodysplasia ossificans progressiva (FOP) is an extremely rare, autosomal dominant transmitted genetic disease. Patients experience progressive bone formation replacing tendons, ligaments, muscle and soft tissue. Cause of FOP are gain-of-function mutations in the Bone Morphogenetic Protein (BMP) receptor Activin A receptor type 1 (ACVR1) (Kaplan et al., 2008). The most common mutation is R206H, which leads to the substitution of codon 206 from arginine to histidine (Shore et al., 2006).Here, we describe the derivation and characterization of two hiPSC lines from two FOP patients, both carrying the mutation R206H. Cells were isolated from urine and reprogrammed using integration free Sendai virus vectors under defined conditions. © 2015 The Authors. Source


Seltmann S.,Charite - Medical University of Berlin | Lekschas F.,Charite - Medical University of Berlin | Muller R.,Charite - Medical University of Berlin | Stachelscheid H.,Charite - Medical University of Berlin | And 7 more authors.
Nucleic Acids Research | Year: 2016

The human pluripotent stem cell registry (hPSCreg), accessible at http://hpscreg.eu, is a public registry and data portal for human embryonic and induced pluripotent stem cell lines (hESC and hiPSC). Since their first isolation the number of hESC lines has steadily increased to over 3000 and new iPSC lines are generated in a rapidly growing number of laboratories as a result of their potentially broad applicability in biomedicine and drug testing. Many of these lines are deposited in stem cell banks, which are globally established to store tens of thousands of lines from healthy and diseased donors. The Registry provides comprehensive and standardized biological and legal information as well as tools to search and compare information from multiple hPSC sources and hence addresses a translational research need. To facilitate unambiguous identification over different resources, hPSCreg automatically creates a unique standardized name for each cell line registered. In addition to biological information, hPSCreg stores extensive data about ethical standards regarding cell sourcing and conditions for application and privacy protection. hPSCreg is the first global registry that holds both, manually validated scientific and ethical information on hPSC lines, and provides access by means of a user-friendly, mobile ready web application. © The Author(s) 2015. Source


Hildebrand L.,Charite - Medical University of Berlin | Hildebrand L.,Berlin Brandenburg Center for Regenerative Therapies | Seemann P.,Charite - Medical University of Berlin | Seemann P.,Berlin Brandenburg Center for Regenerative Therapies | And 13 more authors.
Cellular and Molecular Life Sciences | Year: 2015

Human induced pluripotent stem cells (hiPSC) differentiate into multiple cell types. Selective cell targeting is often needed for analyzing gene function by overexpressing proteins in a distinct population of hiPSC-derived cell types and for monitoring cell fate in response to stimuli. However, to date, this has not been possible, as commonly used viruses enter the hiPSC via ubiquitously expressed receptors. Here, we report for the first time the application of a heterologous avian receptor, the tumor virus receptor A (TVA), to selectively transduce TVA+ cells in a mixed cell population. Expression of the TVA surface receptor via genetic engineering renders cells susceptible for infection by avian leucosis virus (ALV). We generated hiPSC lines with this stably integrated, ectopic TVA receptor gene that expressed the receptor while retaining pluripotency. The undifferentiated hiPSCTVA+ as well as their differentiating progeny could be infected by recombinant ALV (so-called RCAS virus) with high efficiency. Due to incomplete receptor blocking, even sequential infection of differentiating or undifferentiated TVA+ cells was possible. In conclusion, the TVA/RCAS system provides an efficient and gentle gene transfer system for hiPSC and extends our possibilities for selective cell targeting and lineage tracing studies. © 2015 Springer Basel. Source


Rossbach B.,Charite - Medical University of Berlin | Rossbach B.,Berlin Brandenburg Center for Regenerative Therapies | Hildebrand L.,Charite - Medical University of Berlin | Hildebrand L.,Berlin Brandenburg Center for Regenerative Therapies | And 11 more authors.
Stem Cell Research | Year: 2016

We have generated a human induced pluripotent stem cell (iPSC) line derived from urinary cells of 1 28-30. year old healthy female donor. The cells were reprogrammed using a non-integrating viral vector and shown to have full differentiation potential. Together with the iPSC-lines, the donors provided blood cells for the study of immunological effects of the iPSC line and its derivatives in autologous and allogeneic settings. The line is available and registered in the human pluripotent stem cell registry as BCRTi004-A. © 2015. Source


Rossbach B.,Charite - Medical University of Berlin | Rossbach B.,Berlin Brandenburg Center for Regenerative Therapies | Hildebrand L.,Charite - Medical University of Berlin | Hildebrand L.,Berlin Brandenburg Center for Regenerative Therapies | And 11 more authors.
Stem Cell Research | Year: 2016

We have generated a human induced pluripotent stem cell (iPSC) line derived from urinary cells of a 30 year old healthy female donor. The cells were reprogrammed using a non-integrating viral vector and have shown full differentiation potential. Together with the iPSC-line, the donor provided blood cells for the study of immunological effects of the iPSC line and its derivatives in autologous and allogeneic settings. The line is available and registered in the human pluripotent stem cell registry as BCRTi004-A. © 2016 The Authors. Source

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