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North Bergen, NJ, United States

Temple L.M.,James Madison University | Miyamoto D.M.,Drew University | Mehta M.,Drew University | Mehta M.,Johns Hopkins Hospital | And 9 more authors.
Infection and Immunity | Year: 2010

Bordetella avium causes bordetellosis in birds, a disease similar to whooping cough caused by Bordetella pertussis in children. B. avium agglutinates guinea pig erythrocytes via an unknown mechanism. Loss of hemagglutination ability results in attenuation. We report the use of transposon mutagenesis to identify two genes required for hemagglutination. The genes (hagA and hagB) were adjacent and divergently oriented and had no orthologs in the genomes of other Bordetella species. Construction of in-frame, unmarked mutations in each gene allowed examination of the role of each in conferring erythrocyte agglutination, explanted tracheal cell adherence, and turkey poult tracheal colonization. In all of the in vitro and in vivo assays, the requirement for the trans-acting products of hagA and hagB (HagA and HagB) was readily shown. Western blotting, using antibodies to purified HagA and HagB, revealed proteins of the predicted sizes of HagA and HagB in an outer membrane-enriched fraction. Antiserum to HagB, but not HagA, blocked B. avium erythrocyte agglutination and explanted turkey tracheal ring binding. Bioinformatic analysis indicated the similarity of HagA and HagB to several two-component secretory apparatuses in which one product facilitates the exposition of the other. HagB has the potential to serve as a useful immunogen to protect turkeys against colonization and subsequent disease. Copyright © 2010, American Society for Microbiology. All Rights Reserved. Source


Ragheb S.M.,Nile Company for Pharmaceuticals and Chemical Industries | Jimenez L.,Bergen Community College
PDA Journal of Pharmaceutical Science and Technology | Year: 2014

Detection of microbial contamination in pharmaceutical raw materials and finished products is a critical factor to guarantee their safety, stability, and potency. Rapid microbiological methods - such as polymerase chain reaction - have been widely applied to clinical and food quality control analysis. However, polymerase chain reaction applications to pharmaceutical quality control have been rather slow and sporadic. Successful implementation of these methods in pharmaceutical companies in developing countries requires important considerations to provide sensitive and robust assays that will comply with good manufacturing practices. ©PDA, Inc. 2014. Source


Russell G.L.,NASA | Lacis A.A.,NASA | Rind D.H.,NASA | Colose C.,Albany State University | Opstbaum R.F.,Bergen Community College
Geophysical Research Letters | Year: 2013

How does climate sensitivity vary with the magnitude of climate forcing? This question was investigated with the use of a modified coupled atmosphere-ocean model, whose stability was improved so that the model would accommodate large radiative forcings yet be fast enough to reach rapid equilibrium. Experiments were performed in which atmospheric CO2 was multiplied by powers of 2, from 1/64 to 256 times the 1950 value. From 8 to 32 times, the 1950 CO2, climate sensitivity for doubling CO2 reaches 8°C due to increases in water vapor absorption and cloud top height and to reductions in low level cloud cover. As CO2 amount increases further, sensitivity drops as cloud cover and planetary albedo stabilize. No water vapor-induced runaway greenhouse caused by increased CO2 was found for the range of CO2 examined. With CO2 at or below 1/8 of the 1950 value, runaway sea ice does occur as the planet cascades to a snowball Earth climate with fully ice covered oceans and global mean surface temperatures near -30°C. Key Points Atmospheric CO2 controls the Earth's climate Water vapor feedback magnifies the greenhouse effect Climate sensitivity is presently at a local minimum ©2013. American Geophysical Union. All Rights Reserved. Source


Campanella J.J.,Montclair State University | Bologna P.A.X.,Montclair State University | Smalley J.V.,Bergen Community College | Avila D.N.,Montclair State University | And 3 more authors.
Population Ecology | Year: 2013

Within Barnegat Bay, New Jersey, eelgrass (Zostera marina) populations have declined by 62 % over the last 20 years. To better understand the consequences of this devastation, we have previously employed microsatellite DNA polymorphisms to analyze the population structure of Z. marina within Barnegat Bay, as well as along the eastern United States seaboard. We have restored populations of Z. marina in Barnegat Bay over the last 10 years to help assess the best planting conditions and ecotypes that might be used in long-term restoration strategies. In this study, we examined the genetic health of the restored populations compared to that of the donor eelgrass populations within the bay. Using microsatellites, we can identify which parental founding ecotypes survived the restoration process over multiple generations. The frequency of observed heterozygotes, although higher than in the natural populations, still indicates reduced levels of diversity and connectivity. The inbreeding frequency is high in the restored populations, but lower than what is seen in the native populations. All restored populations have effective population values >50, suggesting a high probability of survival in the short term. © 2012 The Society of Population Ecology and Springer Japan. Source


Campanella J.J.,Montclair State University | Zaben N.,Montclair State University | Enriquez D.,Montclair State University | Smalley J.V.,Bergen Community College | Ludwig-Muller J.,TU Dresden
Acta Horticulturae | Year: 2014

We have examined how the ILR1-like auxin conjugate hydrolase gene family has functionally evolved in the gymnosperm species Sitka spruce (Picea sitchensis) and Loblolly pine (Pinus taeda). We have isolated and cloned two orthologues from spruce (PsIAR31, PsIAR32) and one from pine (PtIAR31) that are homologous to the Arabidopsis thaliana AtIAR3 auxin amidohydrolase. We have previously examined the enzymatic activity of PsIAR31 using a thin layer chromatography method. Using more sensitive HPLC methods, we have re-investigated the hydrolytic activity and substrate recognition of both PsIAR31 and PsIAR32. Neither PsIAR31 nor PsIAR32 appears to hydrolyze indole butyric acid (IBA) or indole proprionic acid (IPA) conjugates. Additionally, we have found that PsIAR31 and -32 recognize several IAA conjugates (including IAA-ala, IAA-asp, IAA-gluc, and IAA-isoleu). The PtIAR31 enzyme seems to primarily recognize IBA-alanine as a substrate. Using phylogenetic analysis, we also found a family of five putative paralogue hydrolases in both the pine (PtIAR31-35) and the spruce (PsIAR31-35) genomes. This result supports the hypothesis that multiple copies of auxin conjugate hydrolase genes have been present since gymnosperms evolved, and that this redundancy did not arise later in angiosperms. Source

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