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Qiao A.,Bengbu University | He B.,Nanjing University of Science and Technology | Luo S.,Bengbu University | Huang Y.,Bengbu University | Wang Y.,Bengbu University
Nongye Jixie Xuebao/Transactions of the Chinese Society for Agricultural Machinery | Year: 2017

Installed between the motor's shaft and the hoist power input one, an integrate sensor could output not only the total torque signal related to the change for hoist's load but also 60 pulses per revolution for motor's shaft. The hoist's opening could be calculated by associating the number of those pulses with some parameters of the hoist. In the integrate sensor, those rotary circuits could be powered from the rotary secondary of transformer whose primary was fixed with the sensor's metal shell, which was used as a part of magnetic core of the transformer. By using the photoelectric coupling method, non-contact transmission for the sensor's signals about the total torque and the opening was realized. Besides the torque caused by constant load, the total one also included some other torques shown as fluctuation and unsteadiness because of those factors, such as friction existing in the hoist's mechanical structure. In order to get the actual load from the total torque signal, two phases were selected for hoist's load detection. Firstly, the analog-to-digital converter (ADC) output value corresponding to the total torque was corrected though gray correlation analysis, and the corrected ADC value whose fluctuation and unsteadiness had been greatly reduced was selected to be as the result of the first phase. In the second phase, by using the result of the first phase as input variable, the hoist's load predicted model was founded according to the least square support vector regression (LS-SVR) or partial least square regression (PLSR). Taking the first predicted value as the expected data sequence and implementing the correction based on the gray correlation analysis again, the hoist actual load was finally attained. Experiment result showed that the error of the result of load detection was less than ±0.6% for LS-SVR and below±1% for PLSR model cooperated with the correction based on the gray correlation analysis. Meanwhile, since the integrate sensor was directly installed between the motor shaft and the hoist one, the number of pulses would be greatly increased during the hoist's moving up and down which could well improve the opening detection resolution. The resolution of the hoist opening detection was far below 1 mm. © 2017, Chinese Society of Agricultural Machinery. All right reserved.

Zhang X.,Bengbu University | Lu J.,Wannan Medical College | Li X.,Bengbu University | Chai L.,Bengbu University | Jiao Y.,Bengbu University
New Journal of Chemistry | Year: 2017

We rapidly prepared photoluminescent carbon dots (CDs) using a single-step heat reflux method with l-glutamic acid as the carbon source. The as-prepared CDs possessed a quasispherical morphology with an average diameter of approximately 5.42 nm and a quantum yield of approximately 6.3%. The CDs clearly exhibited excitation-dependent photoluminescence and good temperature-sensitive photoluminescence, as well as excellent water-solubility. Moreover, the luminescent CDs could not only be efficiently taken up by CT26.WT and CAL-27 cells but also exhibited low cytotoxicity and favourable biocompatibility, making them promising candidates for cellular imaging applications, among others. © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique.

Meng P.,Bengbu University
International Journal of Advanced Manufacturing Technology | Year: 2015

Monolayer brazed grinding wheels were prepared using polycrystalline diamond grains, Ni-Cr-B-Si brazing alloy, and commercial Q235A steel with the brazing temperature 1000 °C and the holding time 5 min. The microstructure and element distribution of joint interface was studied through scanning electron microscope and energy dispersive X-ray spectroscope, respectively. Grinding performance of wheels was evaluated during grinding alumina specimen. The fracture of polycrystalline diamond grains with the embedding depth of 50 and 70 % was studied quantitatively. The results show that good joint interface was formed among polycrystalline diamond grains, Ni-Cr-B-Si brazing alloy, and Q235A steel through element diffusion and chemical reaction during brazing. Chemical resultants Cr3C2 and Cr7C3 formed within joint interface between polycrystalline diamond grain and Ni-Cr-B-Si brazing alloy; meanwhile, the growth of Cr3C2 has an orientation relationship with element arrangement of diamond surface. Compared with single crystal diamond grinding wheel, the polycrystalline diamond grinding wheel has exhibited evident advantages according to the grinding force and force ration (tangential grinding force Ft / normal grinding force Fn). The Fn and Ft is 21.69 and 7.23 N with the expositing high of 50 % and 5.16 and 1.72 N with the expositing high of 70 % respectively, when polycrystalline diamond grain fracture takes place. The failure mechanism of brazed polycrystalline diamond grains grinding wheel is diamond fracture. © 2015 Springer-Verlag London

Ge J.,Anhui University | Ge J.,Bengbu University | Liu L.,Anhui University | Shen Y.,Anhui University
Journal of Porous Materials | Year: 2016

The metal–organic frameworks UiO-66 with amine functional groups were synthesized by microwave irradiation, their structures and properties were characterized. The results show that NH2-UiO-66 can be prepared quickly and well-crystallized by microwave-assisted method within 15 min. The amine-functionalized UiO-66 was octahedral crystals of well defined sizes (500–900 nm) and had a high specific surface area about 924.37 m2 g−1 and micropore range from 2.0 to 11 nm. The amine-functionalized UiO-66 was thermally stable up to 540 °C stability and exhibited a good combination of methylene blue adsorption. The maximum adsorption capacity reached up to 203.95 mg g−1. The kinetics and equilibrium of the adsorption process were found to follow the pseudo-second-order kinetic and Langmuir adsorption model, respectively. © 2016 Springer Science+Business Media New York

Ouyang L.-L.,Shanghai Ocean University | Li H.,Bengbu University | Yan X.-J.,Ningbo University | Xu J.-L.,Ningbo University | Zhou Z.-G.,Shanghai Ocean University
Frontiers in Plant Science | Year: 2016

To analyze the contribution of glycerol-3-phosphate acyltransferase (GPAT) to the first acylation of glycerol-3-phosphate (G-3-P), the present study focused on a functional analysis of the GPAT gene from Lobosphaera incisa (designated as LiGPAT). A full-length cDNA of LiGPAT consisting of a 1,305-bp ORF, a 1,652-bp 5′-UTR, and a 354-bp 3′-UTR, was cloned. The ORF encoded a 434-amino acid peptide, of which 63 residues at the N-terminus defined a chloroplast transit peptide. Multiple sequence alignment and phylogeny analysis of GPAT homologs provided the convincible bioinformatics evidence that LiGPAT was localized to chloroplasts. Considering the conservation of His among the G-3-P binding sites from chloroplastidial GPATs and the substitution of His by Arg at position 195 in the LiGPAT mature protein (designated mLiGPAT), we established the heterologous expression of either mLiGPAT or its mutant (Arg195His) (sdmLiGPAT) in the GPAT-deficient yeast mutant gat1Δ. Lipid profile analyses of these transgenic yeasts not only validated the acylation function of LiGPAT but also indicated that the site-directed mutagenesis from Arg195 to His led to an increase in the phospholipid level in yeast. Semi-quantitative analysis of mLiGPAT and sdmLiGPAT, together with the structural superimposition of their G-3-P binding sites, indicated that the increased enzymatic activity was caused by the enlarged accessible surface of the phosphate group binding pocket when Arg195 was mutated to His. Thus, the potential of genetic manipulation of GPAT to increase the glycerolipid level in L. incisa and other microalgae would be of great interest. © 2016 Ouyang, Li, Yan, Xu and Zhou.

Li Z.-Q.,Bengbu University | Yang J.-C.,Bengbu University | Sui K.-W.,Bengbu University | Yin N.,Bengbu University
Materials Letters | Year: 2015

Metal-organic framework Zr6O4(OH)4(BTC)2(HCOO)6 (MOF-808) nanoparticles were successfully synthesized with a household microwave oven in less than 5 min of irradiation time. The obtained MOF-808 nanoparticles exhibited high superacidity, making them excellent adsorbent for arsenic removal. The adsorption capacity of the as-synthesized MOF-808 nanoparticles for arsenic was 24.83 mg/g. Furthermore, the adsorbents still maintained 82.10% of the removal efficiency after five cycles, indicating that the MOF-808 nanoparticles can serve as a regenerable As(V) adsorbent. © 2015 Elsevier B.V. All rights reserved.

Zhang H.,Bengbu University
Computers and Mathematics with Applications | Year: 2015

In this paper, by constructing an objective function and using the gradient search, full-rank and reduced-rank gradient-based algorithms are suggested for solving generalized coupled Sylvester matrix equations. It is proved that the reduced-rank iterative algorithm is convergent for proper initial iterative values. By analyzing the spectral radius of the related matrices, the convergence properties are studied and the optimal convergence factor of the reduced-rank algorithm is determined. The relationship between the reduced-rank algorithm and the full-rank algorithm is discussed. Consequently, the computation load can be reduced greatly for solving a class of matrix equation. A numerical example is provided to illustrate the effectiveness of the proposed algorithms and testify the conclusions suggested in this paper. © 2015 Elsevier Ltd. All rights reserved.

Qian S.,Nanjing Agricultural University | Qian S.,Bengbu University | Lu H.,Nanjing Agricultural University | Meng P.,Nanjing Agricultural University | And 4 more authors.
Bioresource Technology | Year: 2015

The effect of inulin on the production of bacillomycin D and the levels of mRNA of bacillomycin D synthetase genes: bmyA (BYA), bmyB (BYB), bmyC (BYC), the thioesterase gene (TE) and regulating genes: AbrB, ComA, DegU, PhrC, SigmaH and Spo0A in Bacillus subtilis fmbJ were investigated. The production of bacillomycin D was enhanced with the increase of biomass concentration. The maximum production and productivity of bacillomycin D were found to be 1227.49. mg/L and 10.23. mg/L. h. Inulin significantly improved the expression of bacillomycin D synthetase genes: bmyA (BYA), bmyB (BYB), bmyC (BYC) and the thioesterase gene (TE). Also, inulin up-regulated ComA, DegU, SigmaH and Spo0A and therefore promoted the high production of bacillomycin D. Our results provided a practical approach for efficient production of bacillomycin D and a meaningful explanation for regulatory mechanism of bacillomycin D biosynthesis. © 2014 Elsevier Ltd.

Qiao A.,Bengbu University | He B.,Nanjing University of Science and Technology | Wang Y.,Bengbu University
Nongye Jixie Xuebao/Transactions of the Chinese Society for Agricultural Machinery | Year: 2016

The control system of a smart pig porridge feeder was designed based on MCU whose kernel was the ARM Cortex-M3. The control system could well solve the problems of pig porridge feeder which include both the weight proportion between water and dry feeding, and the dry feeding weight delivered for every day. The ways of dynamical adjustment for the weight proportion of water and dry feed were shown as follows. Firstly, the least square support vector regression (LS-SVR) model between the delivered speed of the dry feeding and the motor's rotary speed was founded. Then referred to the LS-SVR model, the abnormal real-time sampling weight value during continuous delivering feed was removed by using some data process methods, such as the threshold value judgment and the Grubbs criterion. At last, the value of feeding at certain times was predicted via the line regression model attained by least squares fitting the rest of sampling data. Because the water flow could be truly detected by a flow sensor, the weight proportion of water and dry feed was well adjusted. Using recursive weight compensation combined the LS-SVR model and static measurement means, the delivered dry feeding weight for one day was accurately controlled by the control system. After being mixed with stirred fully by the feeder using the control system, the water and the dry feed were turned to porridge whose nutrients should be better absorbed by pigs. The weight proportion of water and dry feed error was below 4%. The weight error of feeding for a pig per day was less than 1 g. © 2016, Chinese Society of Agricultural Machinery. All right reserved.

Wu L.,Bengbu University | Zhang X.-F.,Bengbu University | Li Z.-Q.,Bengbu University | Wu F.,Bengbu University
Inorganic Chemistry Communications | Year: 2016

Amino(-NH2) functionalized zirconium metal-organic framework UiO-66 were synthesized in household microwave oven. The structural properties and morphology of the obtained UiO-66-NH2 nanoparticles were characterized by X-ray powder diffraction (XRD), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS). UiO-66-NH2 sensor show high selectivity and sensitivity toward Cu2 + over other tested metal ions. In addition, the possible sensing mechanism is also discussed. © 2016

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