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Biswas M.,Bengal Institute of Pharmaceutical science | Haldar P.K.,Jadavpur University
Tropical Journal of Pharmaceutical Research | Year: 2010

Purpose: Terminalia arjuna Roxb (Combretaceae) is commonly known as Arjjhan and Arjun in Bengal, India. The anti-leishmanial and anticancer activities of a pentacyclic triterpenoid isolated from its leaves were evaluated. Methods: Dried and crushed leaves of Terminalia arjuna were de-fatted with petroleum ether (40 -60°C) and extracted with methanol. The extract was subjected to column chromatography and column fractions were monitored on TLC plates developed in a suitable solvent system. Structures of the isolated pure compounds were elucidated by infra-red spectroscopy, mass spectrometry and nuclear magnetic resonance spectroscopy. One of the compounds was screened for anti-leishmanial activity against promastigotes of Leishmania donovani, and anti-cancer activity on K562 leukaemic cell line. Results: A pentacyclic triterpenoid, characterized as 3β-hydroxyurs-12-en-28-oic acid (together with ß-D-glucoside) and designated ursolic acid, was successfully isolated. The structure was determined on the basis of spectroscopic analyses. In vitro anti-leishmanial activity against promastigotes of Leishmania donovani (strain AG 83) and anti-cancer activity on K562 leukaemic cell line were shown by the isolated ursolic acid. Conclusion: The methanol extract of the leaves of Terminalia arjuna led to the isolation of a pentacyclic triterpenoid, ursolic acid. This compound demonstrated in vitro anti-leishmanial and anti-cancer activities. © Pharmacotherapy Group.


Biswas M.,Bengal Institute of Pharmaceutical science | Haldar P.K.,Jadavpur University
Journal of Natural Science, Biology and Medicine | Year: 2010

This study evaluated the in vitro antioxidant potential of petroleum ether (60-80°C), chloroform, and methanol extract of the fruits of Dregea volubilis Benth (Asclepiadaceae). The different antioxidant assays, including total antioxidant activity, reducing power, free radical, super oxide anion radical, nitric oxide scavenging, lipid peroxidation, and total phenolic content were studied. The extracts exhibited potent total antioxidant activity that increased with increasing amount of extract concentration, which was compared with standard drug vitamin C at different concentrations as extracts. The different concentrations of all the extracts and vitamin C showed inhibition on lipid peroxidation. In addition, all the extracts had effective reducing power, free radical scavenging, super oxide anion scavenging, nitric oxide scavenging, lipid peroxidation, and total phenolic content depending on concentration. These various antioxidant activities were compared with standard antioxidant such as vitamin C at different concentration as different extracts.


Banerjee S.,Bengal Institute of Pharmaceutical science | Debnath P.K.,Gananath Sen Institute of Ayurvdiya and Research
Journal of Traditional and Complementary Medicine | Year: 2015

Ayurveda proclaims food and drugs are intersecting concepts that are vital for human survival and for the prevention and mitigation of diseases. Food interferes with the molecular mechanisms of an organism's "physiome". It is consumed in large amounts compared to any drug. Hence, research on its effect and interaction with genome is highly relevant toward understanding diseases and their therapies. Ayurgenomics presents a personalized approach in the predictive, preventive, and curative aspects of stratified medicine with molecular variability, which embodies the study of interindividual variability due to genetic variability in humans for assessing susceptibility, and establishing diagnosis and prognosis, mainly on the basis of the constitution type of a person's Prakriti. Ayurnutrigenomics is an emerging field of interest pervading Ayurveda systems biology, where the selection of a suitable dietary, therapeutic, and lifestyle regime is made on the basis of clinical assessment of an individual maintaining one's Prakriti. This Ayurveda-inspired concept of personalized nutrition is a novel concept of nutrigenomic research for developing personalized functional foods and nutraceuticals suitable for one's genetic makeup with the help of Ayurveda. Here, we propose and present this novel concept of Ayurnutrigenomics and its emerging areas of research, which may unfold future possibilities toward smart yet safe therapeutics. © 2015 Center for Food and Biomolecules, National Taiwan University.


Biswas M.,Bengal Institute of Pharmaceutical science | Kar B.,Jadavpur University | Kumar R.B.S.,Jadavpur University | Haldar P.K.,Jadavpur University
Pharmaceutical Biology | Year: 2011

Context: Terminalia arjuna Roxb. (Combretaceae), commonly known as Arjuna, is a large tree grown throughout the Indian peninsula and used traditionally for several medicinal purposes. Objective: To evaluate antihyperglycemic and antioxidant role of methanol extract of T. arjuna leaf (META) in Wistar rats. Materials and methods: Hyperglycemia was induced in rats by single intraperitoneal injection of streptozotocin (STZ, 65 mg/kg body weight). Three days after STZ induction, the hyperglycemic rats were treated with META orally at the dose of 100 and 200 mg/kg body weight daily for 15 days. Glibenclamide (0.5 mg/kg, orally) was used as reference drug. The fasting blood glucose levels were measured on every fifth day during the 15-day treatment. Serum biochemical parameters such as serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP), cholesterol, and total protein were estimated. Antioxidant properties were assessed by estimating hepatic lipid peroxidation, reduced glutathione (GSH), and catalase (CAT). Results and discussion: META at the dose of 100 and 200 mg/kg orally significantly (P < 0.001) and dose-dependently reduced and normalized blood glucose levels as compared with that of STZ control group. Serum biochemical parameters were significantly (P < 0.001) restored toward normal levels in META-treated rats as compared with STZ control. META treatment also significantly (P < 0.001) decreased lipid peroxidation and recovered GSH level and CAT activity toward normal as compared with STZ control. Conclusion: The present study infers that T. arjuna leaf demonstrated remarkable antihyperglycemic activity in STZ-induced diabetic rats. The potential antihyperglycemic action is plausibly due to its underlying antioxidant role. © 2011 Informa Healthcare USA, Inc.


PubMed | Bengal Institute of Pharmaceutical science and Rg Kar Medical College And Hospital
Type: Clinical Trial | Journal: Journal of evidence-based complementary & alternative medicine | Year: 2014

The purpose of the study was to assess the health-related quality of life and treatment satisfaction of diabetes patients (n = 36) with foot ulcers undergoing insulin and ayurvedic adjunct therapy. Retrospective hospital based cross-sectional design considered patients with diabetes foot ulcers undergoing 4-week treatment with insulin and ayurvedic combinatorial therapy. The Audit of Diabetes Dependent Quality of Life Questionnaire and Diabetes Treatment Satisfaction Questionnaire were completed for the assessment. The mean duration of diabetes among participants was 9.32 5.3 years. Assessment of Diabetes Treatment Satisfaction Questionnaire (total treatment satisfaction) score was found to be 26.0139 5.20369, and Audit of Diabetes Dependent Quality of Life Questionnaire (average weighted impact) score was -3.0819 1.83003. Relationship with diabetes complications showed that Audit of Diabetes Dependent Quality of Life Questionnaire score (P = .383) had no relationship, but Diabetes Treatment Satisfaction Questionnaire score (P = .039) showed significance. Integrated approaches for diabetes foot ulcer management with insulin and ayurvedic management have a favorable impact on patient-perceived quality of life.


PubMed | Bengal Institute of Pharmaceutical science
Type: Journal Article | Journal: Journal of traditional and complementary medicine | Year: 2016

The effect of an Ayurvedic poly-herbo-mineral formulation Kumarabharana Rasa (KR) in the management of chronic tonsillitis (Tundikeri) in children has been assessed in this study. This clinical study was a double-arm study with a pre- and post-test design at the outpatient level in a tertiary Ayurveda hospital attached to a teaching institute located in district headquarters in Southern India. Patients (n=40) with chronic tonsillitis satisfying diagnostic criteria and aged between 5 and 10 years were selected from the outpatient Department of Kaumarbhritya, SDM College of Ayurveda and Hospital, Hassan. Among them, 20 patients were treated with Kumarabharana rasa (tablet form) at a dose of 500mg once daily for 30 days (Group A). The other 20 patients were treated with Godhuma Vati (placebo) at a dose of 500mg once daily for 30 days (Group B). In both groups, Madhu was the Anupana advised. After completion of 30 days of treatment, the patients were assessed on the following day and another investigation took place 15 days later. Statistically significant effects (p<0.05) in the reduction of all signs and symptoms of chronic tonsillitis after KR treatment were observed. These results indicate that Kumarabharana Rasa has an ameliorative effect in reducing the signs and symptoms of chronic tonsillitis.


PubMed | Gananath Sen Institute of Ayurvdiya and Research and Bengal Institute of Pharmaceutical science
Type: Journal Article | Journal: Journal of traditional and complementary medicine | Year: 2015

Ayurveda proclaims food and drugs are intersecting concepts that are vital for human survival and for the prevention and mitigation of diseases. Food interferes with the molecular mechanisms of an organisms physiome. It is consumed in large amounts compared to any drug. Hence, research on its effect and interaction with genome is highly relevant toward understanding diseases and their therapies. Ayurgenomics presents a personalized approach in the predictive, preventive, and curative aspects of stratified medicine with molecular variability, which embodies the study of interindividual variability due to genetic variability in humans for assessing susceptibility, and establishing diagnosis and prognosis, mainly on the basis of the constitution type of a persons Prakriti. Ayurnutrigenomics is an emerging field of interest pervading Ayurveda systems biology, where the selection of a suitable dietary, therapeutic, and lifestyle regime is made on the basis of clinical assessment of an individual maintaining ones Prakriti. This Ayurveda-inspired concept of personalized nutrition is a novel concept of nutrigenomic research for developing personalized functional foods and nutraceuticals suitable for ones genetic makeup with the help of Ayurveda. Here, we propose and present this novel concept of Ayurnutrigenomics and its emerging areas of research, which may unfold future possibilities toward smart yet safe therapeutics.


PubMed | Tripura University, Bengal Institute of Pharmaceutical science and MBB College
Type: Journal Article | Journal: Journal of advanced pharmaceutical technology & research | Year: 2015

Aldose reductase (AR) plays an important role in the development of several long-term diabetic complications. Inhibition of AR activities is a strategy for controlling complications arising from chronic diabetes. Several AR inhibitors have been reported in the literature. Flavonoid type compounds are shown to have significant AR inhibition. The objective of this study was to perform a computational work to get an idea about structural insight of flavonoid type compounds for developing as well as for searching new flavonoid based AR inhibitors. The data-set comprising 68 flavones along with their pIC50 values ranging from 0.44 to 4.59 have been collected from literature. Structure of all the flavonoids were drawn in Chembiodraw Ultra 11.0, converted into corresponding three-dimensional structure, saved as mole file and then imported to maestro project table. Imported ligands were prepared using LigPrep option of maestro 9.6 version. Three-dimensional quantitative structure-activity relationships and docking studies were performed with appropriate options of maestro 9.6 version installed in HP Z820 workstation with CentOS 6.3 (Linux). A model with partial least squares factor 5, standard deviation 0.2482, R(2) = 0.9502 and variance ratio of regression 122 has been found as the best statistical model.


PubMed | Gujarat Ayurved University and Bengal Institute of Pharmaceutical science
Type: Journal Article | Journal: Ayu | Year: 2014

Jyotishmati (Celastrus paniculatus Willd.) is a woody climber belongs to the family Celastraceae; a well known herbal nootropic, distributed through the tropical and subtropical regions of India. Its leaves are used in eye disease and headache. Very low qualitative and quantitative information about leaves have been documented to establish its quality and purity.Present study was conducted to evaluate physicochemical, phyto-chemical and HPTLC analysis of different solvent extracts of the C. paniculatus leaves.Physico-chemical analysis revealed loss on drying 13.05% w/w, total ash value 16.08% w/w, acid insoluble ash 0.386% w/w, water-soluble extractive 14.22% w/w, alcohol-soluble extractive 9.91% w/w, chloroform-soluble extractive 7.75% w/w and ether-soluble extractive 4.74% w/w. Phytochemical screening showed the presence of steroid and terpenoid in the both pet. ether and ethyl acetate extracts while methanol extract possessed steroid, terpenoid, carbohydrate, alkaloid, saponin, and phenolic compounds.The observations made in this study may help to develop the standards of qualitative and quantitative parameters with regards to identification, quality and purity of C. paniculatus leaf.


Sarkar J.,Bengal Institute of Pharmaceutical science | Debnath M.,Bengal Institute of Pharmaceutical science | Biswas M.,Bengal Institute of Pharmaceutical science
International Journal of Pharmacy and Technology | Year: 2014

ClerodendrumindicumLinn.is an important medicinal plant and widely used in the treatment of variety of diseases and well explored scientifically for their pharmacological properties. The current study was therefore carried out to provide requisite pharmacognostical and physicochemical details of the leaves by means of standardization of the species. The morphological study reveals the leaves are obovate-oblanceolate or elliptic-lanceolate with serrate-dentate margins acute apex and cuneate base. The microscopy reveals the dorsiventral type of leaf, with anomocytic stomata covering lower epidermisandpresence of oil glands were also found. Calcium-oxalate crystals were found within the ground tissue. In the midrib section centrally hollow pith was found, which is surrounded withsclerenchymatous sheath. Physicochemical properties such as ash value and extractive values were established. Qualitative phytochemical screenings revealed the presence of alkaloids, steroids, terpenoids, glycosides, tannins and carbohydrates. The results of the study could be useful in setting some diagnostic indices for the identification and preparation of a monograph of the plant.

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