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Xuan H.,Kompetenzzentrum Obstbau Bodensee KOB | Ding Y.,Beijing Vegetable Research Center | Spann D.,Kompetenzzentrum Obstbau Bodensee KOB | Moller O.,LVWO Weinsberg | And 2 more authors.
Acta Horticulturae | Year: 2011

The aim of this study was, parallel to the usual morphological identification of cultivars, to fingerprint a subset of 45 European plum cultivars with 7 SSR primers, to determine if there was sufficient polymorphism to differentiate the cultivars. And furthermore, to establish one routine method for cultivar identification for European plums at the KOB, and then use this new method as a technique to differentiate two unknown samples, as well as examine the authenticity and purity of cultivars for the nursery industry. The 7 SSR markers generated a total of 140 alleles in the European plum. The number of alleles per locus ranged from 31 for EMPaS01 to 10 for EMPaS02 with an average of 20 alleles per locus. The polymorphism information content (PIC) for the 7 SSR loci varied from 0.857 to 0.94 with an average of 0.898. Based on the PIC values, primer pair EMPaS01 was the most informative while EMPaS02 was the least informative.


Mingchi L.,Beijing Vegetable Research Center | Xiangli L.,China Agricultural University | Jing H.,China Agricultural University | Lihong G.,China Agricultural University
Acta Horticulturae | Year: 2010

Plant growth, photosynthetic and physiological characteristics, fruit quality and yield were studied under simulated drought stress using CaCl 2 solution to improve tomato fruit quality. Under simulated drought stress, water potential and transpiration rate decreased, free water decreased and bound water increased. Photosynthetic rate, stomatal conductance and intercellular CO 2 were reduced. Drought stress improved fruit quality, SSC, organic acid, and vitamin C, increased sugar/acid ratio, but decreased yields. Under simulated drought stress, the number of fruits almost did not change but the weight per fruit was reduced. Dry matter content per fruits increased, and the total dry matter content per section did not change.


Zhao H.,China Agricultural University | Han D.,China Agricultural University | Song S.,Beijing Vegetable Research Center | Chang D.,China Agricultural University
Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering | Year: 2012

For the purposes of detecting the maturity of small watermelon and real-time monitoring its internal quality during the growth, this paper studied a variety of basic information in constantly changing circumstances along with growing days of mini watermelon fruit Jingxiu. During the growth, the majority of the small watermelon basic information changes follow certain regularities and some indicators regularities are similar. Based on the correlation analysis, the factor analysis and the new constructed variable results, it can be found that the quality changes of the small watermelon fruit Jingxiu mainly manifest as the content changes of soluble solids, titratable acidity, flesh moisture and chlorophyll from 20 to 39 days after pollination process. Among these changes, the chlorophyll content changes notably in the course of 22 to 25 days after pollination process, while the other three primarily change in the course of 28 to 30 days. The quality becomes stable during the growth process of 31 to 39 days except some small fluctuations within a small range. The results can provide a basis for selection of parameters for optical non-destructive testing of small watermelon quality and maturity.


PubMed | Beijing Vegetable Research Center and Boyce Thompson Institute for Plant Research
Type: | Journal: BMC genomics | Year: 2015

Pumpkin (Cucurbita maxima Duch.) is an economically important crop belonging to the Cucurbitaceae family. However, very few genomic and genetic resources are available for this species. As part of our ongoing efforts to sequence the pumpkin genome, high-density genetic map is essential for anchoring and orienting the assembled scaffolds. In addition, a saturated genetic map can facilitate quantitative trait locus (QTL) mapping.A set of 186 F2 plants derived from the cross of pumpkin inbred lines Rimu and SQ026 were genotyped using the genotyping-by-sequencing approach. Using the SNPs we identified, a high-density genetic map containing 458 bin-markers was constructed, spanning a total genetic distance of 2,566.8 cM across the 20 linkage groups of C. maxima with a mean marker density of 5.60 cM. Using this map we were able to anchor 58 assembled scaffolds that covered about 194.5 Mb (71.7%) of the 271.4 Mb assembled pumpkin genome, of which 44 (183.0 Mb; 67.4%) were oriented. Furthermore, the high-density genetic map was used to identify genomic regions highly associated with an important agronomic trait, dwarf vine. Three QTLs on linkage groups (LGs) 1, 3 and 4, respectively, were recovered. One QTL, qCmB2, which was located in an interval of 0.42 Mb on LG 3, explained 21.4% phenotypic variations. Within qCmB2, one gene, Cma_004516, encoding the gibberellin (GA) 20-oxidase in the GA biosynthesis pathway, had a 1249-bp deletion in its promoter in bush type lines, and its expression level was significantly increased during the vine growth and higher in vine type lines than bush type lines, supporting Cma_004516 as a possible candidate gene controlling vine growth in pumpkin.A high-density pumpkin genetic map was constructed, which was used to successfully anchor and orient the assembled genome scaffolds, and to identify QTLs highly associated with pumpkin vine length. The map provided a valuable resource for gene cloning and marker assisted breeding in pumpkin and other related species. The identified vine length QTLs would help to dissect the underlying molecular basis regulating pumpkin vine growth.


Snyder J.,University of Kentucky | Min C.,Beijing Vegetable Research Center
Acta Horticulturae | Year: 2012

The core collections of two tomato wild relatives, Lycopersicon hirsutum and L. pennellii, were screened in the open field for resistance to aphids. Nearly all accessions of L. pennellii were resistant to aphids. For the accessions of L. hirsutum, some accessions were very susceptible, some were intermediate and others were extremely resistant. When these lines were being screened for aphid resistance, a line of L. hirsutum, LA2329, was discovered that possessed resistance to leaf miner (Liriomyza spp.). Subsequent attempts to evaluate spidermite and leafminer resistance in this line and in hybrids of LA2329 with cultivated tomato indicated that resistance was dominant and relatively easy to transfer to the hybrids. This is an unusual result since a high level of resistance is not commonly uncovered in early generations of tomato interspecific hybrids. Recovering resistance in early generation backcross populations is equally unusual. In order to study mechanism of resistance, we have conducted spider mite bioassays on a range hybrids of interspecific origin. Progress, including our most recent results with the second backcross gene ration is reviewed.


Pan L.,Hefei University of Technology | Xu X.,Beijing Vegetable Research Center | Luo J.,Hefei University of Technology
Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering | Year: 2011

The effects of ultrasound-assisted extraction on extraction rate and physical characteristics of water insoluble dietary fibers (IDF) were studied. Based on the single factor experiments: the ratio of water to dry soybean dregs powder, ultrasonic power intensity, ultrasonic temperature and extraction time, the four-factor second order rotation combination experimental design was applied to optimize the parameters for IDF of ultrasound-assisted extraction. The physical characteristics of the IDF obtained by technology of ultrasound-assisted extraction (UAE) were examined by a combination of chemical and instrumental analysis approaches, including water-holding, extension, water-binding, fat-binding capacities, SEM, Fourier transform infrared (FT-IR) spectra and TG, and compared with acid dissolution and alkali precipitatiom (ADAP) and enzyme-assisted extraction (EAE). The results indicated that the highest yield of 92.11% was reached under the conditions which the ratio of water to dry soybean dregs power was 35 mL/g, ultrasonic power intensity 600 W/g, ultrasonic temperature 50°C and extraction time 50 min. The IDF obtained by UAE had higher water-holding, extension, water-binding and fat-binding capacities, and much abundant reticular structure than those obtained by ADAP and EAE. The analytic data of FT-IR and TG indicated that ultrasound treatment could raise the free hydroxyl groups, but did not change its pyrolysis mechanism. In conclusion, UAE not only increased the extraction rate of IDF, but also improved physical characteristics.


Ding Y.,Beijing Vegetable Research Center | Jian Y.,Beijing Vegetable Research Center
Acta Horticulturae | Year: 2010

Since nutritional quality of vegetables was increasingly considered to be an important marketing advantage, our cauliflower breeding research efforts were in recent years focused on providing new hybrids high in carotenoid concentration which plays an important role in human nutrition and health. As a result, one F1 hybrid with orange curds was bred. It was crossbred by a cytoplasmatic male sterile line CMS92-60 as female parent and an inbred line 93-4 as male parent. 93-4 was an orange cauliflower mutant bearing low commercial value due to its too small and uncompacted curd. CMS92-60 was a white cauliflower CMS line carrying the cytoplasmatic male sterile gene, which had good combining ability. The results showed that this F1 hybrid was improved in both nutritional value and commercial qualities compared to its parents. It was determined that the content of β-carotene in dry curd was 31.3 μg/g which was 10.8 times more than that of white cauliflower, and the contents of vitamin C, dietary fibre, protein, and mineral elements were nearly equivalent to those of white cauliflower. Each head weighed 800-1000 grams on average, as the level of a commercial variety. It is an early-mature variety, taking about 60 days from transplant to harvest. This hybrid was consequently offered to the growers by the name of 'Jinyu 60'. It is expected to have a positive market advantage in the future.


Xiaoyan Z.,Beijing Vegetable Research Center | Yue M.,Beijing Vegetable Research Center
Acta Horticulturae | Year: 2010

The review summarizes the development of Chinese vegetable production and processing. It indicates that by-products and waste become a crucial problem with reference to main commercial products. It is important that such by-products have functional factors and that they can be utilized; tomato waste is mainly seed and skin which can be resources of dietary fiber and lycopene, carrot pomace contains carotenoids and fiber, asparagus by-products can be made into functional juice, and artichoke waste is a good resource of polyphenols. The market of Chinese functional food is also described and the future work is discussed as well.


Wu L.,Beijing Vegetable Research Center | Baohai Z.,Beijing Vegetable Research Center | Yong X.,Beijing Vegetable Research Center
Acta Horticulturae | Year: 2010

Beijing will hold the 2008 Olympic Games during the rainy season which is characterized by high temperature and high levels of humidity. This time of the year, between August and September is also known for the low level of vegetable production and the high infestation rate from pests. Under such difficult conditions, the BOCOG (Beijing Organizing Committee for the Games of XXIX Olympiad) will strive to meet the requirements on vegetable types, quantity and quality for supply to the many athletes and other participants of the Olympics. One of key services prescribed by the BOCOG and People's Government of Beijing Municipality is to guarantee a safe supply of vegetables for the games.


PubMed | Beijing Vegetable Research Center
Type: Journal Article | Journal: Plant cell reports | Year: 2013

Isolated microspores of various populations of three varieties of the Chinese cabbage pakchoi (Brassica rapa ssp. chinensis) were cultivated in vitro on NLN82 medium (Lichter 1982) and embryos and plantlets obtained with nine cultivars. The best embryo yield per bud was 57.4. A 33C one day heat treatment was generally necessary to induce embryogenesis. Analysis of ploidy level through flow cytometry for two cultivars indicated that haploids were present.

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