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Li B.,Beijing University of Chinese Medicine | Zhang Y.H.,The Southern District of Beijing Stomatological Hospital | Wang L.X.,Beijing Stomatological Hospital | Li X.,The Second Artillery General Hospital of Beijing | Zhang X.D.,Hebei Medical University
Genetics and Molecular Research | Year: 2015

This study aims to investigate the expression changes of RANKL, RUNX2, and OPG in rabbit periodontal tissues under orthodontic force and explore its effect on the remodeling of periodontal tissues. A total of 16 specific pathogen-free rabbits were used in this study. The maxillary appliance was worn on the right (experimental) side, and the appliance-free left side was used as the control. Rabbits were sacrificed after 3, 5, 7, and 14 days of treatment. Changes in the expression levels of OPG, RANKL, and RUNX2 in the periodontium were detected using real-time PCR and western blotting methods. The OPG expression levels decreased after 3 to 14 days of treatment, while the expression levels of RANKL and RUNX2 increased after 3 to 14 days. The OPG expression levels decreased while those of RANKL and RUNX2 increased during orthodontic tooth movement,which suggested that they play a role in the osteogenesis process and the reconstruction of periodontal tissue. © FUNPEC-RP. Source


Wang X.,Tsinghua University | You Z.,Tsinghua University | Sha H.,Beijing Stomatological Hospital | Cheng Y.,Qingdao University of Science and Technology | And 2 more authors.
Talanta | Year: 2014

A DNA and graphene (GR) bi-layer modified carbon ionic liquid electrode (CILE) was fabricated by an electrodeposition method. GR nanosheets were electrodeposited on the surface of CILE at the potential of -1.3 V and then DNA was further deposited at the potential of +0.5 V on GR modified CILE. Electrochemical performances of the fabricated DNA/GR/CILE were carefully investigated. Then electrochemical behaviors of dopamine (DA) on the modified electrode were studied with the calculated electrochemical parameters. Under the optimized conditions, a linear relationship between the oxidation peak current and the concentration of DA was obtained in the range from 0.1 μmol/L to 1.0 mmol/L with a detection limit of 0.027 μmol/L (3σ). The modified electrode exhibited excellent reproducibility, repeatability, stability, validation and robustness for the electrochemical detection of DA. The proposed method was further applied to the DA injection solution and human urine samples determination with satisfactory results. © 2014 Elsevier B.V. All rights reserved. Source


Wang X.,Tsinghua University | Cheng Y.,Qingdao University of Science and Technology | You Z.,Tsinghua University | Sha H.,Beijing Stomatological Hospital | And 3 more authors.
Ionics | Year: 2015

In this paper, a new electrochemical oxalic acid (OA) sensor based on graphene (GR)-modified carbon ionic liquid electrode (CILE) was proposed. The GR nanosheets were deposited on the surface of CILE by electroreduction with graphene oxide as precursor and electrocatalytic oxidation of OA on GR/CILE was further investigated. As compared with that of bare CILE, the oxidation peak current of OA on GR/CILE were greatly improved with the decrease of the oxidation potential. Electrochemical parameters of the electrooxidation of OA were calculated. Under the selected conditions, the oxidation peak currents increased with OA concentration in the range from 8.0 μM to 6.0 mM with the detection limit as 0.48 μM (3σ). The proposed method exhibited higher sensitivity and wider linear range, which was applied to determination of OA concentration in spinach samples with satisfactory results. © 2014, Springer-Verlag Berlin Heidelberg. Source


Wang X.,Tsinghua University | You Z.,Tsinghua University | Sha H.,Beijing Stomatological Hospital | Sun Z.,Qingdao University of Science and Technology | Sun W.,Qingdao University of Science and Technology
Journal of Solid State Electrochemistry | Year: 2014

In this paper, a Fe3O4@SiO2 core-shell structure microsphere was synthesized and used to investigate the direct electron transfer of myoglobin (Mb) with a 1-butylpyridinium hexafluorophosphate based carbon ionic liquid electrode (CILE) as the substrate electrode. The mixture of Mb and Fe3O4@SiO2 microsphere could form an organic-inorganic composite, which was immobilized on the surface of CILE with a chitosan (CS) film. Cyclic voltammetric experiments indicated that a pair of well-defined quasi-reversible redox peaks appeared on CS/Mb-Fe 3O4@SiO2/CILE with the formal peak potential (E 0′) located at -0.31 V (vs. saturated calomel electrode), which was corresponded to the electroactive center of Mb heme Fe(III)/Fe(II) redox couples. Direct electrochemical behaviors of Mb in CS-Fe3O 4@SiO2 composite film were carefully investigated with the electrochemical parameters calculated. The CS/Mb-Fe3O 4@SiO2/CILE showed good electrocatalytic behaviors to the reduction of trichloroacetic acid in the concentration range from 0.2 to 11.0 mmol L-1 with the detection limit of 0.18 mmol L-1 (3σ). Based on CS/Mb-Fe3O4@SiO2/CILE, a new third-generation reagentless electrochemical biosensor was constructed with higher sensitivity and reproducibility. © 2013 Springer-Verlag Berlin Heidelberg. Source


Wang X.,Tsinghua University | You Z.,Tsinghua University | Cheng Y.,Qingdao University of Science and Technology | Sha H.,Beijing Stomatological Hospital | And 3 more authors.
Journal of Molecular Liquids | Year: 2015

In this study a new electrochemical folic acid (FA) sensor was proposed by using gold nanoparticles (AuNPs) and graphene (GR) modified carbon ionic liquid electrode (CILE) as the working electrode. GR and AuNPs were directly electroreduced on the surface of CILE to get the modified electrode, which exhibited excellent electrochemical performances due to the specific characteristics including higher conductivity and larger surface area. The modified electrode showed excellent electrocatalytic ability to the oxidation of FA with an irreversible oxidation peak appeared at + 1.05 V (vs. SCE) in pH 5.5 phosphate buffer solution. Electrochemical behaviors of FA on the modified electrode were carefully investigated with the electrochemical parameters calculated. Under the optimal conditions and by using differential pulse voltammetry, the oxidation peak currents were linear to FA concentration in the range from 0.01 μmol L- 1 to 50.0 μmol L- 1 with the detection limit as 2.7 nmol L- 1 (3σ). The proposed method was successfully applied to the FA tablet sample determination with satisfactory results. © 2015 Elsevier B.V. All rights reserved. Source

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