Beijing Stomatological Hospital

Beijing, China

Beijing Stomatological Hospital

Beijing, China
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Zhang W.,Beijing Stomatological Hospital | Li C.,Capital Medical University | Xing X.,Beijing ChaoYang Integrative Medicine Emergency Medical Center | Li X.,Beijing ChaoYang Integrative Medicine Emergency Medical Center | And 3 more authors.
NeuroReport | Year: 2017

This study aimed to explore brain activation in patients with cleft lip and palate (CLP) using a Chinese character subvocalization task, in which the stimuli were selected from a clinical articulation evaluation test. CLP is a congenital disability. Individuals with CLP usually have articulation disorder caused by abnormal lip and palate structure. Previous studies showed that primary somatosensory and motor areas had a significant difference in activation in patients with CLP. However, whether brain activation was restored to a normal level after palatoplasty and speech rehabilitation is not clear. Two groups, adults after palatoplasty with speech training and age-matched and sex-matched controls, participated in this study. Brain activation during Chinese character subvocalization task and behavioral data were recorded using functional MRI. Patients with CLP responded to the target significantly more slowly compared with the controls, whereas no significant difference in accuracy was found between the groups. Brain activation had similar patterns between groups. Broca's area, Wernicke's area, motor areas, somatosensory areas, and insula in both hemispheres, and the dorsolateral prefrontal cortex and the ventrolateral prefrontal cortex in the right hemisphere were activated in both groups, with no statistically significant difference. Furthermore, the two-sample t-test showed that the hippocampus in the left hemisphere was activated significantly in patients with CLP compared with the controls. The results suggested that the hippocampus might be involved in the language-related neural circuit in patients with CLP and play a role of pronunciation retrieval to help patients with CLP to complete the pronunciation effectively. © 2017 Wolters Kluwer Health, Inc. All rights reserved.


Li B.,Beijing University of Chinese Medicine | Zhang Y.H.,The Southern District of Beijing Stomatological Hospital | Wang L.X.,Beijing Stomatological Hospital | Li X.,The Second Artillery General Hospital of Beijing | Zhang X.D.,Hebei Medical University
Genetics and Molecular Research | Year: 2015

This study aims to investigate the expression changes of RANKL, RUNX2, and OPG in rabbit periodontal tissues under orthodontic force and explore its effect on the remodeling of periodontal tissues. A total of 16 specific pathogen-free rabbits were used in this study. The maxillary appliance was worn on the right (experimental) side, and the appliance-free left side was used as the control. Rabbits were sacrificed after 3, 5, 7, and 14 days of treatment. Changes in the expression levels of OPG, RANKL, and RUNX2 in the periodontium were detected using real-time PCR and western blotting methods. The OPG expression levels decreased after 3 to 14 days of treatment, while the expression levels of RANKL and RUNX2 increased after 3 to 14 days. The OPG expression levels decreased while those of RANKL and RUNX2 increased during orthodontic tooth movement,which suggested that they play a role in the osteogenesis process and the reconstruction of periodontal tissue. © FUNPEC-RP.


Wang X.,Tsinghua University | You Z.,Tsinghua University | Sha H.,Beijing Stomatological Hospital | Gong S.,Qingdao University of Science and Technology | And 2 more authors.
Microchimica Acta | Year: 2014

A nanohybrid biomaterial was fabricated by mixing Co3O4 nanorods, gold nanoparticles (Au-NPs) and myoglobin (Mb), and depositing it on the surface of a carbon paste electrode containing the ionic liquid N-hexylpyridinium hexafluorophosphate as the binder. UV-vis and FT-IR revealed the Mb in the composite film to have remained in its native structure. A pair of well-defined redox peaks appears in cyclic voltammograms and indicates direct electron transfer from the Mb to the underlying electrode. The results are attributed to the favorable orientation of Mb in the composite film, to the synergistic effects of Co3O4 nanorods and Au-NPs. The modified electrode shows excellent electrocatalytic ability towards the reduction of substrates such as trichloroacetic acid and nitrite, and displays good stability and reproducibility. [Figure not available: see fulltext.] © 2013 Springer-Verlag Wien.


Wang X.,Tsinghua University | Cheng Y.,Qingdao University of Science and Technology | You Z.,Tsinghua University | Sha H.,Beijing Stomatological Hospital | And 3 more authors.
Ionics | Year: 2015

In this paper, a new electrochemical oxalic acid (OA) sensor based on graphene (GR)-modified carbon ionic liquid electrode (CILE) was proposed. The GR nanosheets were deposited on the surface of CILE by electroreduction with graphene oxide as precursor and electrocatalytic oxidation of OA on GR/CILE was further investigated. As compared with that of bare CILE, the oxidation peak current of OA on GR/CILE were greatly improved with the decrease of the oxidation potential. Electrochemical parameters of the electrooxidation of OA were calculated. Under the selected conditions, the oxidation peak currents increased with OA concentration in the range from 8.0 μM to 6.0 mM with the detection limit as 0.48 μM (3σ). The proposed method exhibited higher sensitivity and wider linear range, which was applied to determination of OA concentration in spinach samples with satisfactory results. © 2014, Springer-Verlag Berlin Heidelberg.


Wang X.,Tsinghua University | You Z.,Tsinghua University | Sha H.,Beijing Stomatological Hospital | Cheng Y.,Qingdao University of Science and Technology | And 2 more authors.
Talanta | Year: 2014

A DNA and graphene (GR) bi-layer modified carbon ionic liquid electrode (CILE) was fabricated by an electrodeposition method. GR nanosheets were electrodeposited on the surface of CILE at the potential of -1.3 V and then DNA was further deposited at the potential of +0.5 V on GR modified CILE. Electrochemical performances of the fabricated DNA/GR/CILE were carefully investigated. Then electrochemical behaviors of dopamine (DA) on the modified electrode were studied with the calculated electrochemical parameters. Under the optimized conditions, a linear relationship between the oxidation peak current and the concentration of DA was obtained in the range from 0.1 μmol/L to 1.0 mmol/L with a detection limit of 0.027 μmol/L (3σ). The modified electrode exhibited excellent reproducibility, repeatability, stability, validation and robustness for the electrochemical detection of DA. The proposed method was further applied to the DA injection solution and human urine samples determination with satisfactory results. © 2014 Elsevier B.V. All rights reserved.


Wang X.,Tsinghua University | You Z.,Tsinghua University | Sha H.,Beijing Stomatological Hospital | Sun Z.,Qingdao University of Science and Technology | Sun W.,Qingdao University of Science and Technology
Journal of Solid State Electrochemistry | Year: 2014

In this paper, a Fe3O4@SiO2 core-shell structure microsphere was synthesized and used to investigate the direct electron transfer of myoglobin (Mb) with a 1-butylpyridinium hexafluorophosphate based carbon ionic liquid electrode (CILE) as the substrate electrode. The mixture of Mb and Fe3O4@SiO2 microsphere could form an organic-inorganic composite, which was immobilized on the surface of CILE with a chitosan (CS) film. Cyclic voltammetric experiments indicated that a pair of well-defined quasi-reversible redox peaks appeared on CS/Mb-Fe 3O4@SiO2/CILE with the formal peak potential (E 0′) located at -0.31 V (vs. saturated calomel electrode), which was corresponded to the electroactive center of Mb heme Fe(III)/Fe(II) redox couples. Direct electrochemical behaviors of Mb in CS-Fe3O 4@SiO2 composite film were carefully investigated with the electrochemical parameters calculated. The CS/Mb-Fe3O 4@SiO2/CILE showed good electrocatalytic behaviors to the reduction of trichloroacetic acid in the concentration range from 0.2 to 11.0 mmol L-1 with the detection limit of 0.18 mmol L-1 (3σ). Based on CS/Mb-Fe3O4@SiO2/CILE, a new third-generation reagentless electrochemical biosensor was constructed with higher sensitivity and reproducibility. © 2013 Springer-Verlag Berlin Heidelberg.


Yu S.,Capital Medical University | Yu S.,Beijing Stomatological Hospital | Long J.,University of Sichuan | Yu J.,Nanjing Medical University | And 6 more authors.
Cells Tissues Organs | Year: 2013

PDLSC-mediated periodontal tissue regeneration is considered a promising method for periodontitis treatment, but the supply of PDLSCs is limited. As a potential alternative, WJCMSCs are readily available; however, there is a lack of evidence proving that WJCMSCs are suitable for periodontal tissue regeneration. In this study, we investigated the characteristics of WJCMSCs and PDLSCs. We found the osteo-/dentinogenic, adipogenic and chondrogenic differentiation potentials of PDLSCs were more powerful than those of WJCMSCs. Microarray analysis discovered that 903 genes were significantly down-regulated and 726 genes up-regulated in WJCMSCs compared with PDLSCs. Based on the microarray data, we found that several genes may be associated with MSCs characteristics. Further bioinformatic analysis identified that TGF-β and WNT signaling pathways, and several genes, including STAT5B and ITGA4, may play key roles in MSCs. Our results indicate that the differentiation potentials of WJCMSCs are far less than those of PDLSCs, and that unmodified WJCMSCs may not be good seeding cells for periodontal tissue regeneration. Our results also help to elucidate the differentiation mechanisms in MSCs and to find the key factors to prompt WJCMSC-mediated periodontal tissue regeneration. Copyright © 2012 S. Karger AG, Basel.


Shi Y.,Tsinghua University | Ren C.,Beijing Stomatological Hospital | Hao W.,Beijing Stomatological Hospital | Zhang M.,Tsinghua University | And 2 more authors.
IEEE Sensors Journal | Year: 2012

A silicon piezoresistive stress sensor is developed to measure the three components of the orthodontic forces of a tooth in invisible aligners. The stress sensor chip consists of multiple piezoresistor rosettes, and is fixed on the tooth surface to measure the orthodontic force induced stresses. An inverse problem algorithm is established to reconstruct the orthodontic forces of the tooth from the measured stresses. A key thinning process is developed using chemical-mechanical polishing to thin the sensor chip from 650 μm to 100 μm, such that it can be accommodated to the limited space between the aligner and the tooth, without much influence on the orthodontic force. The ultra-thin thickness enables the sensor chip to be compliance with tooth deformation, and improves the stress measurement sensitivity as much as 40 times, making it capable of measuring small orthodontic force. The sensor design, fabrication, orthodontic stress measurement, and force reconstruction are given in details, and the experimental results demonstrate the feasibility of using miniaturized and ultra-thin sensors to determine the in vitro orthodontic forces. © 2012 IEEE.


PubMed | P.A. College, Beijing Institute of Biotechnology, Dalian Medical University, Anhui Medical University and 2 more.
Type: Journal Article | Journal: Molecular medicine reports | Year: 2015

Four and a half LIM protein 1 (FHL1) has been characterized as a tumor suppressor in various types of tumor. However, the biological function and underlying mechanism of FHL1 in tongue squamous cell carcinoma (TSCC) remain to be elucidated. The present study demonstrated that FHL1 inhibits anchoragedependent and independent growth of TSCC cells in vitro and tumor growth in nude mice, as determined by cell proliferation and soft agar assays. Knockdown of FHL1 with FHL1 small interfering RNA (siRNA) promoted tumor growth in nude mice. Mechanistically, flow cytometric analysis showed that knockdown of FHL1 promoted G1/S cell cycle progression. Furthermore, expression of cell cycleassociated regulators, cyclin D and cyclin E, were detected by western blotting and reverse transcriptionquantitative polymerase chain reaction. Cyclin D and cyclin E were markedly elevated at both the protein and mRNA level in the FHL1 siRNAtransfected cells. These results suggested that FHL1 has a tumor suppressive role in TSCC and that FHL1 may be a useful target for TSCC gene therapy.


PubMed | Beijing Stomatological Hospital, Beijing University of Chinese Medicine, The Southern District of Beijing Stomatological Hospital, The Second Artillery General Hospital of Beijing and Hebei Medical University
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

This study aims to investigate the expression changes of RANKL, RUNX2, and OPG in rabbit periodontal tissues under orthodontic force and explore its effect on the remodeling of periodontal tissues. A total of 16 specific pathogen-free rabbits were used in this study. The maxillary appliance was worn on the right (experimental) side, and the appliance-free left side was used as the control. Rabbits were sacrificed after 3, 5, 7, and 14 days of treatment. Changes in the expression levels of OPG, RANKL, and RUNX2 in the periodontium were detected using real-time PCR and western blotting methods. The OPG expression levels decreased after 3 to 14 days of treatment, while the expression levels of RANKL and RUNX2 increased after 3 to 14 days. The OPG expression levels decreased while those of RANKL and RUNX2 increased during orthodontic tooth movement, which suggested that they play a role in the osteogenesis process and the reconstruction of periodontal tissue.

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