Beijing Saisheng Pharmaceutical Co.
Beijing Saisheng Pharmaceutical Co.
Wang A.,Peking University |
Wang A.,Beijing Saisheng Pharmaceutical Co. |
Shang M.,Peking University |
You R.,Qinghai University for Nationalities |
And 3 more authors.
Journal of Chinese Pharmaceutical Sciences | Year: 2015
Sinopodophylli Fructus is the commonly used traditional Tibetan medicinal herb. In the present study, we established a reversedphase high performance liquid chromatography method to simultaneously determine three lignans and five flavonoid constituents, namely podophyllotoxin, desoxypodophyllotoxin, 4'-demethyldesoxypodophyllotoxin, 8-prenylkaemferol, quercetin, kaempferol, 8,2'-diprenylquercetin 3-methylether and 8-prenylquercetin, in Sinopodophylli Fructus. The chromatographic separation was achieved on a C18 analytical column with a gradient mobile phase consisting of acetonitrile and 0.05% phosphoric acid at a flow rate of 1.0 mL/min. UV detection was set at 290 nm and 370 nm, and the column oven was set at 35°C. This method provided a good reproducibility, and its overall intra- and inter-day precision was less than 3% and 4%, respectively. The recovery of the method was 98.29%-101.60%, and a good linearity (R2≥0.9992) was obtained for all the analytes over a relatively wide range of concentration. A total of 17 samples of S. hexandrum (12 fruits, 5 roots and rhizomes) were collected from different areas and then successfully quantified. The results indicated that the contents of eight compounds significantly varied (the sum content ranged from 16.90 to 55.68 mg/g), and prenylated flavonoids could be used as marker constituents in the identification and quality control of Sinopodophylli Fructus. © 2015 Journal of Chinese Pharmaceutical Sciences, School of Pharmaceutical Sciences, Peking University.
Li X.-F.,Beijing Saisheng Pharmaceutical Co. |
Kong S.-Q.,Beijing Saisheng Pharmaceutical Co. |
Zhai Z.-F.,Beijing Saisheng Pharmaceutical Co. |
Song M.-W.,Beijing Saisheng Pharmaceutical Co. |
Ma B.,Beijing Saisheng Pharmaceutical Co.
Chinese Journal of Biologicals | Year: 2015
Objective: To develop and verify a method for determination of residual tributyl phosphate content in bovine fibrinogen. Methods: Capillary gas chromatography was adopted under the following conditions: chromatographic column: DA-FFAP modified cross-linked polyethylene glycol capillary chromatographic column-20M (30 m × 0.25 mm × 0.25 μm); solvent: n-hexane; temperature of vaporizing chamber: 190 °C; temperature of detector: 210 °C; temperature of column: 140 °C; carrier gas: highly pure nitrogen (99.999%); flow rate: 1.0 ml/min, no diversion; sample loading: 0.1 μl. The quantitative and minimum detection limits of tributyl phosphate were determined. The method was verified for precison, linearity and accuracy, and used for determination of residual tributyl phosphate content in three batches of samples. Results: The quantitative detection limit and minimum detection limit of tributyl phosphate were 0.830 6 and 0.207 6 ng respectively. The RSI) of peak areas of six sample loadings was 2.138 6%, indicating high precision of the method. The linear range of tributyl phosphate was 2.129 0 ∼ 17.032 0 μg/ml, while the regression equation was y = 5.317 7 x + 1.096 1, with a R2 value of 0.997 3. The mean recovery rates of samples at low, moderate and high dosages were 100.596 9%, 98.597 5% and 99.712 5%, with RSDs of 0.641 2%, 0.553 7% and 1.009 5%, respectively. The residual tributyl phosphate contents in three batches of samples were 3.259 6, 3.287 9 and 3.193 3 (Jig/ml respectively. Conclusion: Gas chromatography was simple, accurate and reliable, which might be used for the determination of residual tributyl phosphate content in blood products.