Time filter

Source Type

Yang P.,U.S. Center for Disease Control and Prevention | Yang P.,Beijing Research Center for Preventive Medicine | Thompson M.G.,Centers for Disease Control and Prevention | Ma C.,U.S. Center for Disease Control and Prevention | And 9 more authors.
Vaccine | Year: 2014

Background: Influenza vaccine coverage remains low in China, and there is limited information on the preventive value of local vaccination programs. Methods: As part of influenza virological surveillance in Beijing, China during the 2012-2013 influenza season, we assessed the vaccine effectiveness (VE) of one or more doses of trivalent inactivated influenza vaccine (IIV3) in preventing medically-attended influenza-like-illness (ILI) associated with laboratory-confirmed influenza virus infection using a test-negative case-control design. Influenza vaccination was determined based on self-report by adult patients or the parents of child patients. Results: Of 1998 patients with ILI, 695 (35%) tested positive for influenza viruses, including 292 (42%) A(H3N2), 398 (57%) A(H1N1)pdm09, and 5 (1%) not (sub)typed influenza viruses. The rate of influenza vaccination among all patients was 4% (71/1998). Among influenza positive patients, 2% (57/1303) were vaccinated compared to 4% (14/695) among influenza negative patients, resulting in VE for one or more doses of vaccine (adjusted for age, sex, week, and days since illness onset) against all circulating influenza viruses of 52% (95% CI = 12-74%). A significant adjusted VE for one or more doses of vaccine for all ages against A(H1N1)pdm09 of 59% (95% CI, 8-82%) was observed; however, the VE against A(H3N2) was 43% (95% CI, -30% to 75%). The point estimate of VE was 59% (95% CI, 19-79%) for those aged <60 years, but a negative VE point estimate without statistical significance was observed among those aged ≥60 years. Conclusions: IIV3 conferred moderate protection against medically-attended influenza in Beijing, China during the 2012-2013 season, especially against the A(H1N1)pdm09 strain and among those aged <60 years old. © 2014 Elsevier Ltd.


Yin J.,Beijing Research Center for Preventive Medicine | Wang H.,Beijing Research Center for Preventive Medicine | Zhang J.,Beijing Research Center for Preventive Medicine | Zhou N.,China National Center for Biotechnology Development | And 4 more authors.
Chemosphere | Year: 2012

Human breast milk samples collected from mothers (n=110) who lived in Chengdu, Sichuan Province, southwestern China in 2009 were analyzed to determine the concentrations of 13 musk compounds. Possible relationships between musk concentrations and some personal characteristics were also studied. Only five target analytes were detected in the milk samples analyzed, with median concentration values of 16.5, 11.5, 7.85, <1.5 and <1.4ngg -1lipid weight for AHTN (7-acetyl-1,1,3,4,4,6-hexamethyl-1,2,3,4-tetrahydronaphthalene), HHCB (1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta[γ]-2-benzopyran), HHCB-lactone (1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta[γ]-2-benzopyran-1-one), OTNE ([1,2,3,4,5,6,7,8-octahydro-2,3,8,8-tetramethylnaphthalen-2yl]ethan-1-one) and musk ketone (4-tert-butyl-2,6-dimethyl-3,5-dinitroacetophenone, MK), respectively. Mothers who reported high use of hand-cleaning agents, body-cleaning agents, shampoo and hair conditioners, hair dyes and hair gels had significantly elevated milk concentrations of HHCB whereas elevated milk concentrations of AHTN were observed among mothers reporting high use of body-cleaning agents, body lotions, shampoos, hair dyes and hair gels. Younger age showed a significantly positive effect on milk concentrations of both HHCB and AHTN whereas BMI after delivery, the number of children nursed and place of residence (urban or rural) had no significant effect. The estimated median daily intakes of synthetic musks for breast-fed infants were considerably lower than the current provisional tolerable daily intake amounts suggested for adults. © 2011 Elsevier Ltd.


Yang Y.,Beijing Research Center for Preventive Medicine | Guan J.,U.S. Center for Disease Control and Prevention | Yin J.,Beijing Research Center for Preventive Medicine | Shao B.,Beijing Research Center for Preventive Medicine | Li H.,Beijing Research Center for Preventive Medicine
Chemosphere | Year: 2014

The use of bisphenol A (BPA) has been restricted in many countries because of its potential health effects. As a result of these restrictions, a group of bisphenol analogues that are structurally similar to BPA have been developed as the alternatives for industrial applications. However, latest researches indicated that these chemicals have similar endocrine-disrupting effects as BPA in humans. Moreover, only a limited number of studies have attempted to monitor the exposure level in humans of other bisphenol analogues. In the present study, the concentrations of seven bisphenols, including bisphenol S (BPS), bisphenol F (BPF), bisphenol B (BPB), BPA, bisphenol AF (BPAF), tetrachlorobisphenol A (TCBPA) and tetrabromobisphenol A (TBBPA), in human urine samples were measured by liquid chromatography coupled to mass spectrometry (LC-MS/MS) following the enzymatic hydrolysis of glucuronidase/arylsulfatase and liquid-liquid extraction (LLE). Under the optimised conditions, high recoveries (81.6-116.8%) were obtained for all the analytes, and the relative standard deviations (RSD, %) were less than 16.4% (n=6). The isotopic internal standard calibration curves for each of the target compounds exhibited excellent linearity (r2>0.99) and the limit of quantification (LOQ) for the analytes in urine ranged from 0.024 to 0.310ngmL-1. The method was applied to investigate the urinary levels of these seven bisphenols in a cohort of residents living near a BPAF manufacturing plant in south China. BPS, BPF, BPA and BPAF were detected in urine samples at concentrations ranging from


PubMed | National Institute for Nutrition and Health, U.S. Center for Disease Control and Prevention and Beijing Research Center for Preventive Medicine
Type: Journal Article | Journal: Biomedical and environmental sciences : BES | Year: 2015

To determine ten B-vitamins in human milk by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).The pretreated human milk samples were adequately separated and quantified within 11 min by UPLC-MS/MS with an Acquity UPLC HSS T3 column (2.1100 mm, 1.8 m). The mobile phase was a gradient of 2.5 mmol/L ammonium formate aqueous solution and acetonitrile at a flow rate of 0.35 mL/min. Stable isotope internal standards were used in the analysis, to correct for the method variability, including matrix and ionization effects. The homogenized human milk samples were deproteinzed using methanol, unknown contaminants were extracted with diethyl ether and hydrophobic phase was discarded. The analytes were monitored via ESI+ionization and detected in multiple reaction monitoring (MRM) with three acquisition functions.Calibration curves ranged from 0.5-160 ng/mL (thiamin, riboflavin, biotin, nicotinic acid, pyridoxine, pyridoxamine, pyridoxal), and 2.5-800 ng/mL (pantothenic acid, FAD and nicotinamide) (R2=0.990-0.999). The relative recovery ranged from 80.1% to 120.2%; accuracy was determined to be 98.3% to 108.0%. Intra-day and inter-day variation were 3.4%-19.9% and 5.9%-18.1%, respectively. The limit of quantification (LOQ) for all vitamins was between 0.25 and 3 g/L.This method was successfully applied for simultaneous analysis of ten B-vitamins in human milk.


Yang Y.,Beijing Research Center for Preventive Medicine | Lu L.,Beijing Research Center for Preventive Medicine | Zhang J.,Beijing Research Center for Preventive Medicine | Wu Y.,China National Center for Food Safety and Risk Assessment | Shao B.,Beijing Research Center for Preventive Medicine
Journal of Chromatography A | Year: 2014

This article presents a simple and universal analytical method for the simultaneous analysis of bisphenol S (BPS), bisphenol F (BPF), bisphenol A (BPA), bisphenol B (BPB), bisphenol AF (BPAF), tetrachlorobisphenol A (TCBPA), and tetrabromobisphenol A (TBBPA) in environmental water (river water, sewage) and solid samples (sediment, sludge) based on liquid chromatography-electrospray tandem mass spectrometry (LC-MS/MS). Analytes were extracted from water samples using hydrophilic lipophilic balanced (HLB) solid-phase extraction (SPE) cartridges, and the extracts were further purified using MAX SPE cartridges. For the solid samples, a combination of ultrasonic extraction with the same SPE clean-up procedures used for the water samples was employed. The absolute recoveries for all analytes in the water and solid samples ranged from 57.1 to 114.3%. Good method reproducibility was achieved in terms of intra- and inter-day precision, yielding relative standard deviations (RSDs) less than 16.9 and 18.1%, respectively. The method limits of quantitation (MLOQ) for the seven compounds in environmental water and solid samples ranged from 0.05 to 4.35. ng/L and from 0.06 to 2.83. ng/g (dry weight, d.w.), respectively. Finally, this method was successfully applied to real environmental sample analysis, which revealed that all of the tested BPs were present, with the exception of BPB. © 2014 Elsevier B.V.


Yin J.,Beijing Research Center for Preventive Medicine | Niu Y.,Beijing Research Center for Preventive Medicine | Shao B.,Beijing Research Center for Preventive Medicine
Journal of Environmental Sciences (China) | Year: 2016

Methotrexate (MTX) is a cytotoxic drug widely used in the treatment of tumors, autoimmune diseases and severe asthma. jen00883This drug has been frequently detected in the aquatic environment with concentrations up to μg/L levels. The MTX present in environmental water might be transformed and removed during chlorination disinfection treatment. In this work, the fate of MTX during aqueous chlorination was investigated in laboratory batch experiments, and the transformation products of MTX were identified. Aqueous solutions of MTX (1mg/L) were chlorinated by sodium hypochlorite solution at room temperature under neutral pH conditions. Chlorinated products were pre-concentrated with solid-phase extraction (SPE) cartridges and determined by liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). The reaction of MTX chlorination exhibited pseudo-first-order kinetics and the half-life time of MTX degradation was calculated to be 1.65min, when the initial chlorine concentration was 2mg/L. Two chlorinated MTX congeners, 4-amino-3-chlorinated-N10-methylpteroylglutamic (monochloro-MTX) and 4-amino-3,5-dichloro-N10-methylpteroylglutamic (dichloro-MTX) were found in the chlorinated solution. Monochloro-MTX was successfully fractionated by high performance liquid chromatography (HPLC) and its structure was further identified using 1H nuclear magnetic resonance (NMR) analysis. The presence of the two products in real hospital wastewater was then examined and both compounds were detected. Finally, the effects of MTX and monochloro-MTX on the cell cycle progression in vitro were evaluated using zebrafish liver cell line. It was found that both compounds could inhibit the proliferation of zebrafish liver cells through S phase arrest and their effects on the cell cycle profile had no significant difference. © 2016.


Ren X.,National Institute for Nutrition and Health | Yang Z.,National Institute for Nutrition and Health | Shao B.,Beijing Research Center for Preventive Medicine | Yin S.-A.,National Institute for Nutrition and Health | Yang X.,National Institute for Nutrition and Health
PLoS ONE | Year: 2015

To determine the contents of B-vitamins in human milk in China, we analyzed 1778 human milk samples from the sample bank of the National High Technique R & D Program (863 Projects) which was a cross-sectional survey and covered 6419 human milk samples from healthy lactating mothers who were at different stages of lactation (0-330 days postpartum) in 11 provinces of China. The contents of free forms of six B-vitamins in these human milk samples were analyzed by using UPLC-MS/MS. The median concentrations of free form of 6 B-vitamins in colostrums, transitional milk, 15-180 d mature milk and 181-330 d mature milk were respectively as follows: thiamin 5.0 μg/L, 6.7 μg/L, 21.1 μg/L and 40.7 μg/L; riboflavin 29.3 μg/L, 40.6 μg/L, 33.6 μg/L and 29.6 μg/L; niacin 470.7 μg/L, 661.3 μg/L, 687.0 μg/L and 571.3 μg/L; vitamin B-6 4.6 μg/L, 16.1 μg/L, 62.7 μg/L and 80.7 μg/L; flavin adenine dinucleotide (FAD) 808.7 μg/L, 1162.8 μg/L, 1023.9 μg/L and 1057.2 μg/L; pantothenic acid 1770.9 μg/L, 2626.8 μg/L, 2213.0 μg/L and 1895.5 μg/L. The contents of 6 B-vitamins varied significantly among the different lactation stages and different areas (coastal area vs inland area, rural area vs urban area). The present study indicated that the concentrations of B-vitamins in colostrum were generally much lower than those in transitional milk and mature milk. Further studies are warranted for their roles and significance on B-vitamins in colostrum in nutrition and metabolism of neonates. Copyright: © 2015 Ren et al.


Yang Y.,Beijing Research Center for Preventive Medicine | Yu J.,Beijing Research Center for Preventive Medicine | Yin J.,Beijing Research Center for Preventive Medicine | Shao B.,Beijing Research Center for Preventive Medicine | Zhang J.,Beijing Research Center for Preventive Medicine
Journal of Agricultural and Food Chemistry | Year: 2014

This study aimed to develop a selective analytical method for the simultaneous determination of seven bisphenol analogues in beverage and canned food samples by using a new molecularly imprinted polymer (MIP) as a sorbent for solid-phase extraction (SPE). Liquid chromatography coupled to triple-quadruple tandem mass spectrometry (LC-MS/MS) was used to identify and quantify the target analytes. The MIP-SPE method exhibited a higher level of selectivity and purification than the traditional SPE method. The developed procedures were further validated in terms of accuracy, precision, and sensitivity. The obtained recoveries varied from 50% to 103% at three fortification levels and yielded a relative standard deviation (RSD, %) of less than 15% for all of the analytes. The limits of quantification (LOQ) for the seven analytes varied from 0.002 to 0.15 ng/mL for beverage samples and from 0.03 to 1.5 ng/g for canned food samples. This method was used to analyze real samples that were collected from a supermarket in Beijing. Overall, the results revealed that bisphenol A and bisphenol F were the most frequently detected bisphenols in the beverage and canned food samples and that their concentrations were closely associated with the type of packaging material. This study provides an alternative method of traditional SPE extraction for screening bisphenol analogues in food matrices. © 2014 American Chemical Society.


PubMed | China National Center for Food Safety Risk Assessment and Beijing Research Center for Preventive Medicine
Type: Journal Article | Journal: Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment | Year: 2016

The levels of 12 synthetic musks and one musk metabolite in 24 pooled human milk samples were examined in order to assess the health risks of these contaminants to breast-feeding infants of China. The 24 pooled samples comprised of 1237 individual human milk samples collected from 12 provinces of China according to WHO guidelines. Among the 13 target analytes, OTNE ([1,2,3,4,5,6,7,8-octahydro-2,3,8,8-tetramethylnaphthalen-2yl]ethan-1-one), HHCB (1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta[]-2-benzopyran), AHTN (7-acetyl-1,1,3,4,4,6-hexamethyl-1,2,3,4-tetrahydronaphthalene), musk ketone (4-tert-butyl-2,6-dimethyl-3,5-dinitroacetophenone, MK), Musk T (1,4-dioxacyclohepta decane-5,17-dione), HHCB-lactone (1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethyl cyclopenta[]-2-benzopyran-1-one) and musk ambrette (1-(1,1-dimethylethyl)-2-methoxy-4-methyl-3,5-dinitrobenzene, MA), were found in the milk samples analysed with mean (median) concentrations of 3.96 (3.91), 18.03 (15.10), 10.30 (9.38), 4.68 (4.45), <3.70 (<3.70), 10.02 (9.20) and <5.20 (<5.20) ngg(-1) lipid weight, respectively, whereas ADBI (4-acetyl-1,1-dimethyl-6-tert-butylindan), AHDI (6-acetyl-1,1,2,3,3,5-hexamethylindan), ATII (5-acetyl-1,1,2,6-tetramethyl-3-isopropylindan), musk xylene (1-tert-butyl-3,5-dimethyl-2,4,6-trinitrobenzene, MX), musk tibetene (1-tert-butyl-3,4,5-trimethyl-2,6-dinitrobenzene, MT) and musk moskene (1,1,3,3,5-pentamethyl-4,6-dinotroindane, MM) were not detected. Significantly positive associations were observed in concentration levels between HHCB and AHTN (p<0.001), HHCB and HHCB-lactone (p<0.05), AHTN and HHCB-lactone (p<0.001), and MK and OTNE (p<0.05). No statistical difference was found in musk concentrations between rural and urban areas in China (p>0.05). The mean (median) estimated daily intakes by infants were 20.5 (20.2), 93.4 (78.2), 53.4 (48.6), 24.2 (23.0) and 51.9 (47.6) ngkg(-1) body weight for OTNE, HHCB, AHTN, MK and HHCB-lactone, respectively. The musk exposure levels of infants in China via breast feeding were very low according to the current toxicological information.


PubMed | Capital Medical University and Beijing Research Center for Preventive Medicine
Type: Journal Article | Journal: Environmental geochemistry and health | Year: 2016

Triclosan (TCS) and triclocarban (TCC) exposures are highly concerned due to their suspected endocrine-disrupting effects. The present study investigated TCS and TCC exposure levels in the general Chinese population by biomonitoring human urine and nail samples. TCS (69-80%) and TCC (99-100%) were frequently detected, which demonstrates that the general Chinese population has extensive exposure to these chemicals. The geometric mean (GM) urinary concentrations were 0.40g/g creatinine (creat), 95% confidence interval (CI) 0.30-0.56, for TCS and 0.40g/g creat, 95% CI 0.29-0.56, for TCC. On the other hand, the GM levels of TCS and TCC were 13.57 (5.67g/kg) and 84.66g/kg (41.50g/kg) in fingernail (toenail) samples, respectively, indicating that the levels in fingernails were approximately twice as high as those in toenails. Pearsons correlation coefficients between the urine and fingernail (toenail) samples were 0.715 (0.614) for TCS and 0.829 (0.812) for TCC. These data suggest that nail samples can be applied to the biomonitoring for TCS and TCC in the general population. We observed that the levels of both chemicals were higher in females than in males for urine and fingernail samples, but no significant differences were found between different genders for either compound in toenails. Nineteen- to 29-year-olds had the highest TCS levels in their nail samples, whereas TCC levels did not differ with regard to age. Region of residence significantly influenced TCS and TCC concentrations in the three biological matrices measured.

Loading Beijing Research Center for Preventive Medicine collaborators
Loading Beijing Research Center for Preventive Medicine collaborators