Beijing Rehabilitation Center

Beijing, China

Beijing Rehabilitation Center

Beijing, China
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Xie J.-K.,Capital Medical University | Xie J.-K.,Beijing Rehabilitation Center | Jiang D.-C.,Capital Medical University
Chinese Pharmaceutical Journal | Year: 2013

OBJECTIVE: To look for gene polymorphism loci influencing valproic acid interindividual differences, thus to provide support for treating epilepsy patients with valproic acid. METHODS: Through the KEGG Database, genes and their loci influencing valproic acid pharmacokinetics and pharmacodynamics were inquired from the perspective of pathway: literature was searched about valproic acid-related gene polymorphisms: the FastSNP was used to estimate the risk of the genes found from KEGG Database and literature: the dbSNP database was used to find the distribution of the gene loci with higher risk in Chinese and then to select the loci for the experimental study. RESULTS: The related gene inquired from the perspective of pathway were ABAT, CACNA1I, CACNA1H, CACNA1G, CDY2B, CDY1B, CDY1, CDY2A, CYP2C9, and CYP3A4. Through literature search and bioinformatics methods, 29 influencing loci on valproic acid were identified, including rs1641021, rs1731017, and rs1057910, and so on. CONCLUSION: From this bioinformatics research, the gene polymorphism loci affecting valproic acid interindividual difference are successfully screened. This study provides genetics basis for individual treatments of epileptic patients.

Wang H.-M.,Beijing Hongtianji Neuroscience Academy | Wang H.-M.,Beijing Rehabilitation Center | Ren Y.-S.,Beijing Hongtianji Neuroscience Academy | Tian G.-Z.,Beijing Hongtianji Neuroscience Academy | And 6 more authors.
Acta Anatomica Sinica | Year: 2010

Objective: To establish a technical method to isolate and culture human olfactory mucosa cells in vitro, and to study the immunological characteristics of olfactory ensheathing cells (OECs). Methods: In this study, seven adult olfactory mucosae were obtained from nasal cavity surgery. Cells were acquired by dissection of olfactory mucosa and purified according to the different attachment time among the various cell types. The cells were observed for morphological characteristics under inverted microscope and identified using immunofluorescence staining with p75, S100 and glial fibrillary acidic protein (GFAP). The characteristics of olfactory ensheathing cells was observed under confocal microscope. Results: S100 and p75 positive cells were more common in the olfactory mucosa cells under confocal microscope, GFAP positive cells were relatively rare. In general, S100 and p75 were co-expressed in the same cells, but not the GFAP and S100, p75 expressions. The p75 positive cells presented two completely different morphology. The percentage of S100 and p75 double labeled cells was (35.0 ± 8.3)% under confocal microscope. Conclusion: The olfactory ensheathing cells can be isolated from human olfactory mucosa, they are accessible sources for autologous grafting to repair nervous system disease.

Shi X.,Peking University | Kang Y.,Beijing Hongtianji Neuroscience Academy | Kang Y.,Beijing Rehabilitation Center | Hu Q.,Peking University | And 9 more authors.
Brain Research | Year: 2010

Trials of neuroprotection in ischemic stroke mainly concern gray matter protection, with little information on white matter damage. Olfactory ensheathing cells (OECs) are capable of providing continuous neurotrophic factor and of promoting growth and remyelination of damaged axons. We evaluated OECs for the repair of white matter after transplantation in middle cerebral artery occlusion (MCAO) rat. OECs were cultured from the olfactory bulbs of adult green fluorescent protein (GFP)-expressing transgenic rats and transplanted into peri-infarct basal ganglia imminently after reperfusion. The mortality, neurological score, and function were record everyday until 56 days. At 24 h and 56 days, the infarction volume, the Luxol-fast blue (LFB) staining, and Neurofilament (NF) immunohistochemistry and Western blot were performed to assess the demyelination and remyelination in white matter. OECs transplantation reduced the infarct volume, decreased mortality, and improved neurological deficits in 56 days after MCAO. LFB marked myelin, NF-positive immunohistochemistry, and Western blot indicated that the remyelination and axon regeneration in OEC transplanted rat were significant. In conclusion, OECs can protect the white matter from ischemic injury, but the potential mechanisms of transplanted OEC-mediated recovery need further studies to identify. © 2009 Elsevier B.V. All rights reserved.

Cao J.-L.,Beijing Hongtianji Neuroscience Academy | Liu J.-Y.,Beijing Hongtianji Neuroscience Academy | Xiao J.,Beijing Hongtianji Neuroscience Academy | Jiang X.-R.,Beijing Hongtianji Neuroscience Academy | And 7 more authors.
Acta Anatomica Sinica | Year: 2010

Objective: To observe the survival and migration of olfactory ensheathing cells (OECs) after transplanted into amyloid precursor protein (APP) transgenic mice and investigate its therapeutical effect on Alzheimer disease. Methods: OECs from olfactory ensheathing bulb of enhanced green fluorescent protein ( EGFP ) transgenic mice were isolated, then transplanted into an APP transgenic mice. Stereotaxic coordinates and anterior-posterior were appilicated for microinjection. Confocal microscope was used to observe the survival and migration of the cells, immunohistochemistry and Western blotting were carried out to survey amyloid burden, 4 mice in each group. Results: The results showed that OECs survived well and migrated away from injection site after transplanted into APP transgenic mice, the vascular phenomena of OECs was also observed, and the therapy reduced amyloid burden of Alzheimers disease model. Quantitative test showed that APP reduced significantly (P < 0.05). Conclusion: Olfactory ensheathing cells do some help to reduce the amyloid burden in the brain of APP transgenic mice.

Li Y.,Beijing Hongtianji Neuroscience Academy | Li Y.,Taishan Medical University | Bao J.,Beijing Hongtianji Neuroscience Academy | Bao J.,First Affiliated Hospital of Xin Jiang Medical University Urumqi | And 10 more authors.
Anatomical Record | Year: 2011

Amyotrophic lateral sclerosis (ALS) is a progressively fatal, incurable, neurodegenerative disorder. In this study, we investigated whether olfactory ensheathing cells (OEC) transplantation could provide protection to motor neurons and enable remyelination in mutant SOD1G93A transgenic rats with ALS. Seventy-two rats were divided into four groups: SOD1G93A rats (n = 20); medium+SOD1G93A rats (n = 20); OECs+SOD1G93A rats (n = 24); and another eight wild-type rats were used as controls. About 5 μL (1 × 105) OECs in DF12 medium was injected into the dorsal funiculus of the thoracic spinal cord at a predetermined depth. Survival analysis revealed a significant increase in the survival time in OEC+SOD1G93A rats. Body weight records and inclined board test showed a significant difference between OEC+SOD1G93A and SOD1G93A from the onset at 7 days to 11 days (P < 0.05). Four weeks following transplantation, motor neuron counts in the ventral horn of the spinal cord noted a significant motor neuron loss in SOD1G93A rats when compared with wild-type rats (P < 0.001), and much less neuronal loss and collapse was noted in OEC+SOD1G93A rats when compared with SOD1G93A rats(P < 0.001); immunohistochemistry and Western blot analysis of choline acetyltransferase supported the motor neuron count. Images of confocal microscope indicated that the transplanted OECs had survived for more than 4 weeks and migrated 4.2 mm through the spinal cord. Evidence of remyelination of transplanted OEC was captured with triple fluorescence labeling of green fluorescent protein, neurofilament, and myelin basic protein and was further confirmed by Western blot analysis of MPB. In conclusion, the transplanted OECs could serve as a source of neuroprotection and remyelination to modify the ALS microenvironment. © 2011 Wiley-Liss, Inc.

Yang H.-Q.,Beijing Rehabilitation Center | Zhang M.-Y.,Xi'an Jiaotong University | Dang X.-Q.,Xi'an Jiaotong University | Wang K.-Z.,Xi'an Jiaotong University
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: As a bone scaffold material, β-tricalcium phosphate has good biocompatibility, osteoinductive, and biomechanical properties. Objective: To study the effect of allogeneic osteoblasts compounded with β-tricalcium phosphate in repairing rabbit radial defects. Methods: A total of 45 rabbit radial defect models were made and divided into three groups in random. Experimental group was repaired with the compound of allogeneic osteoblasts and β-tricalcium phosphate; control group with β-tricalcium phosphate; and blank control group with nothing. The new bone formation of each group was observed and assessed by X-ray and histopathological analysis at weeks 4, 8, 16 after implantation for evaluation of the bone repairing effect. Results and Conclusion: With the repair time, the experimental group appeared to complete bone defect repair gradually. By the end of 16 weeks, the X-ray showed that the bone callus between the scaffold and the host was completely ossified, and bone defects were completely repaired in the experimental group. Histopathological observation showed continuous cortical bone formed in the defect area, and canal recanalization realized in the experimental group. Additionally, the repair effect in the experimental group was better than that in the control and blank control group at different time points (P < 0.01). It is suggested that the allogenic osteoblasts/β-tricalcium phosphate compound has the better effects on guiding bone regeneration and preventing from nonunion.

Hou Z.-W.,Beijing University of Chinese Medicine | Li Y.-L.,Beijing Hongzhi Middle School | Zhang L.-H.,Beijing Rehabilitation Center | Yu H.,Beijing University of Chinese Medicine | And 2 more authors.
Chinese Journal of Integrative Medicine | Year: 2014

Objective: To observe the physiological and psychological effects of Chinese traditional healthcare exercises (CTHE) on the adolescents. Methods: A total of 136 healthy students of junior school were recruited and randomly divided into the test group (68 cases) and the control group (68 cases). The subjects in the test group practiced CTHE, while those in the control group did "the 8th radio calisthenics", an official recommended calisthenics for promoting healthcare in China, 3 times a week, and 7 weeks practicing overall. The general body function examination and the meridian energy detection system were used to determine the effects of the two groups. Results: (1) After exercise, the chest circumference and heart rate were increased significantly (P<0.01), and both the systolic pressure (SP) and diastolic pressure (DP) were decreased significantly (P<0.01), while the weight had no significant change (P>0.05) in the test group. In the control group, the chest circumference, the SP and DP had no significant improvement (P>0.05), while the heart rate was significantly increased and the weight was significantly decreased (P<0.01). (2) The test group achieved significant positive changes in the performance status, state of mind and fatigue index (P<0.01 or P<0.05), while the control group only achieved a significant positive change in performance status (P<0.01), and a negative significant decrease in the autonomic nerve function (P<0.05). Conclusion: Junior school students would get physiological and psychological benefit from practicing CTHE, and which is suitable for them to practice. © 2014 Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag.

Liang Y.-H.,Capital Medical University | Liang Y.-H.,Beijing Rehabilitation Center | Li P.,Capital Medical University | Zhao J.-X.,Capital Medical University | And 2 more authors.
Journal of Chinese Integrative Medicine | Year: 2010

Objective: In order to reveal the treatment mechanism of Chinese medicine with the effect of activating blood and resolving putridity, we selected acetyl-11-keto-beta-boswellic acid (AKBA) and arsenic trioxide (ATO), the main monomeric components of frankincense and arsenolite which are two most commonly used Chinese medicine with effect of activating blood and resolving putridity. We combined AKBA and ATO as a compound, and explored its regulatory role in productions and activities of matrix metalloproteinase (MMP)-1, MMP-2 and MMP-9 in human skin fibroblasts (HSFbs) and human acute monocytic leukemia cell line THP-1 in inflammatory state. Methods: In order to simulate the inflammatory micro-environment of chronic wounds, we established 3 cell models: HSFb model activated by tumor necrosis factor-alpha (TNF-α), THP-1 cell model activated by phorbol-12-myristate-13-acetate (PMA) and HSFb-THP-1 cell coculture system. AKBA and ATO were cocultured with these cell models. Enzyme-linked immunosorbent assay (ELISA), gelatin zymography assay and reverse transcription-polymerase chain reaction (RT-PCR) were used to test the secretions, activities and mRNA expressions of MMP-1, MMP-2 and MMP-9. In the study of the regulatory mechanism of AKBA and ATO on MMPs, AKBA and ATO were cocultured with the cell models. ELISA was used to test the secretions of TNF-α and interleukin-1beta (IL-β) and Western blot was used to test the phosphorylation levels of extracellular signal-regulated kinases 1 and 2 (ERK1/2) and p38 mitogen-activated proteinkinase (p38MAPK). Results: Compound of AKBA and ATO inhibited MMP-1, MMP-2 and MMP-9 mRNA expressions, secretions and activities respectively in HSFbs and THP-1 cells in inflammatory state (P < 0.05, P < 0.01). Also compound of AKBA and ATO inhibited secretions of TNF-α and IL-1β in THP-1 cells and cell coculture system (P<0.01). It also decreased the phosphorylation of ERK1/2 and p38 MAPK in HSFbs and THP-1 cells (P<0.05, P<0.01). Conclusion: The combined use of AKBA and ATO which in line with the rule of activating blood and resolving putridity inhibits fibroblasts and inflammatory cells in producing MMPs in inflammatory state through inhibiting the release of inflammatory factors and MAPK cascade pathway.

Yang Z.-Q.,Beijing Rehabilitation Center | Zhang Q.-M.,Beijing Rehabilitation Center
Chinese Journal of Contemporary Pediatrics | Year: 2013

For the children who suffer trauma in earthquake, rehabilitation care aims to promote functional recovery shorten hospital stay, and reduce the incidence of complications or disability by evidence-based, multidisciplinary and comprehensive early rehabilitation intervention on the basis of first aid and clinical treatment. Children are likely to suffer traumatic brain injury, spinal cord injury, peripheral nerve injury, limb fracture, and amputation in the earthquake disaster, so the clinical rehabilitation care designed considering the characteristics of children should be provided immediately alter acute phase of trauma to promote functional recovery.

Liang Y.-H.,Capital Medical University | Liang Y.-H.,Beijing Rehabilitation Center | Li P.,Capital Medical University | Zhao J.-X.,Capital Medical University | And 2 more authors.
Chinese Medical Journal | Year: 2012

Background The elevated matrix metalloproteinase (MMP) activity is an important cause of chronic wound healing failure. Arsenolite, whose main component is arsenic trioxide (AS2O3), is a common traditional Chinese medicine wildly used in treating chronic wounds; it can remove necrotic tissue and promote tissue regeneration. This research was designed to evaluate the effects of AS2O3 on production and activities of MMP-1, MMP-2 and MMP-9, and on regulation of its signal transduction pathway in human skin fibroblasts (HSFb) and human monocyte line (THP-1 cells) that were in an inflammatory state. Methods We established three cell models; HSFb activated by TNF-α, THP-1 cells activated by phorbol 12-myristate 13-acetate (PMA) and an HSFb-THP-1 co-culture system. Three cell models was cultured with AS2O3 for 24 hours. The levels of MMP-1, MMP-2, MMP-9, TNF-α and IL-1β in the cell culture supernatants were assayed by ELISA. The mRNA expressions of MMP-1, MMP-2 and MMP-9 were determined by RT-PCR. The activities of MMP-2 and MMP-9 were tested by Gelatin zymography assays. The phosphorylation levels of ERK1/2 and p38MAPK were assayed by Western blotting. Results AS2O3 inhibited the expression of MMP-1, MMP-2 and MMP-9 mRNA, the secretion and activity of MMP-1, MMP-2 and MMP-9 in HSFb and THP-1 cells in the inflammatory state (P <0.05 and P <0.01 respectively). It also inhibited the secretion of TNF-α and IL-1β in THP-1 cells and in the co-culture system (P <0.05 and P <0.01, respectively). It also decreased the phosphorylation of ERK1/2 and p38 MAPK in HSFb and THP-1 cells (P<0.05 and P <0.01, respectively). Conclusions AS2O3, as a main component of arsenolite, can inhibit the production of MMPs by HSFb and THP-1 cells in an inflammatory state through inhibiting the release of inflammatory factors and the activation of the MAPK cascade pathway. This may be a possible mechanism for arsenolite healing chronic wounds.

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