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Ma J.,Beijing Polytechnic
Lecture Notes in Electrical Engineering | Year: 2013

There has been fierce competition in the express delivery market in China with its fast development. This competition is mostly focused on lower service price which results in poor customer service in the industry, especially for private companies. Thus, express companies should carefully observe the demands of consumers and take service quality into strong consideration. This paper aims to provide features of consumers' demand based on a survey of 147 corporations in Beijing. From this survey, various concerns about service quality emerged including delayed delivery, high damage rate to packages, and poor service attitude. The paper focuses on the micro level rather than the macro level of the express delivery market. The questionnaire was designed to measure not only the satisfaction degree of current services but also the features of service demand required. The results in this paper can be useful for express companies when they design service products or place marketing strategies. © 2013 Springer-Verlag London.

Liu Z.H.,Beijing Polytechnic
Advanced Materials Research | Year: 2014

In the entire life-cycle of manufacturing of a new product, a great number of multimediaresources are generated, which is belong to manufacturing resources too. How to manage and fully sharing these multimediaresources is a key research part for cloud manufacturing (CMfg), which is aim at realize the full sharing and on-demand use of various manufacturing resources. In this paper, after introduce the concept of cloud computing and cloud manufacturing, a cloud based multimediaresources management and sharing method is proposed, as well as is architecture. © (2014) Trans Tech Publications, Switzerland.

Chen Z.,Beijing University of Chemical Technology | Li Y.,Beijing Polytechnic | Yuan Q.,Beijing University of Chemical Technology
International Journal of Biological Macromolecules | Year: 2015

Chondroitinase ABC I (ChSase ABC I) which could degrade chondroitin sulfate (CS) to low molecular weight CS was expressed with His-tag in Escherichia coli (E. coli) BL21(DE3). The effect of His-tag on ChSase ABC I was investigated compared with ChSase ABC I which cut His-tag for the first time. After three steps purification, the specific activity of His-ChSase ABC I was 201.9±5.4IU/mg which was two times lower than ChSase ABC I. Results of multi angle light scattering (MALS) and analytical ultracentrifugation (AUC) showed that the polymeric state of His-ChSase ABC I was not effected by His-tag and it was monomer, and ChSase ABC I was the same. The optimal temperature and pH of His-ChSase ABC I were 37°C and 7.5, and were almost same with ChSase ABC I. Vmax and kcat/Km of His-ChSase ABC I were 2.4±0.1μmol/Ls, and 22.2±0.4L/(μmols) and catalytic efficiency was lower than ChSase ABC I. Generally, His-tag had no effect on polymeric state, optimal temperature and pH, had little negative impact on specific activity, kcat/Km and secondary-structure of ChSase ABC I. This study might guide the application of ChSase ABC I in industrial production. © 2015 Elsevier B.V.

Chen Z.,Beijing University of Chemical Technology | Li Y.,Beijing Polytechnic | Yuan Q.,Beijing University of Chemical Technology
International Journal of Biological Macromolecules | Year: 2015

Chondroitinase ABC I (ChSase ABC I) which can degrade chondroitin sulfate (CS) and other glycosaminoglycan to oligosaccharide or unsaturated disaccharide, was fusionally expressed with maltose-binding protein (MBP) in Escherichia coli BL21(DE3) (E. coli BL21(DE3)) and purified for the first time in this study. The result showed that the productivity of recombinant MBP-ChSase ABC I was 3180IU/(L fermentation liquor) with CS A as substrate, and the productivity might be the highest level when compared to the reported ones. The specific activity of recombinant MBP-ChSase ABC I was 76IU/(mg protein) after purification. The Vmax, Km and kcat were 18.7±0.3μmol/Ls, 73.1±4.1μmol/L and 586.7±10.8s-1, respectively. Enzyme activity of the purified enzyme remained about 78% after 210min when the enzyme incubated at 30°C. This study introduces a rapid method for highly expressing ChSase ABC I, and the method could be adopted in the process of industrial production. Furthermore the investigation of thermostability might lead to an important guide in clinical treatment. © 2014 Elsevier B.V.

Yang H.,Beijing Polytechnic
Open Automation and Control Systems Journal | Year: 2015

As an object-oriented dynamic scripting language, Python has been widely applied in scientific computation, image processing and network communication fields, etc., however, there is no enough attention paid to it in automated test. A Python-based client/server auto-dial testing mode which is successfully applied in actual wireless network optimization testing is introduced. The actual application shows that this mode brings flexible and high-efficiency features of Python programming into full play and significantly improves the efficiency of testing system. © Hongxue Yang.

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