Beijing Municipal Key Laboratory of Clinical Epidemiology

Fengtai, China

Beijing Municipal Key Laboratory of Clinical Epidemiology

Fengtai, China
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Yuan L.,Capital Medical University | Yuan L.,Beijing Municipal Key Laboratory of Clinical Epidemiology | Zhou X.,Capital Medical University | Li D.,East China University of Science and Technology | And 5 more authors.
Neuroscience Letters | Year: 2012

Pattern recognition receptors (PRRs) play important roles in the inflammatory responses to Alzheimer's disease (AD). Our previous study indicated that soybean isoflavone (SIF) exhibited anti-inflammatory effect in rats treated by β-amyloid peptides1-42 (Aβ1-42). In present study, we further detected the effects of SIF against inflammation caused by Aβ1-42 treatment in rats. Serum inflammatory mediators and neurotrophic factors including transforming growth factor-β (TGF-β), inducible nitric oxide synthase (iNOS), brain-derived neurotrophic factor (BDNF) and S100β were detected by enzyme-like immunosorbent assay (ELISA). Reverse transcription-polymerase chain reaction (RT-PCR) and western blot methods were applied for detecting mRNA and protein expression of interleukin-1β (IL-1β), iNOS, tumor necrosis factor-α (TNF-α), TGF-β, BDNF, S100β, myeloid differentiation factor88 (Myd88), Toll-like receptor2 (TLR2), formyl peptide receptors (FPRs), inhibitor κB kinase (IKK) and inhibitor κB-α (IκB-α) in rat's brain tissue. Our results indicated that SIF could reduce the production of IL-1β, TNF-α and iNOS induced by Aβ1-42 in serum and brain of rats. SIF also significantly reversed Aβ1-42-induced up-regulation of TLR2, FPR, Myd88, IKK and decreased IκB-α mRNA and protein expressions in rats. These results suggested that TLR2 and FPR might involve in the inflammatory process induced by Aβ1-42 treatment, and SIF was an efficiency compound in reversing the inflammation caused by Aβ1-42 treatment. © 2011 Elsevier Ireland Ltd.


Wang N.,Capital Medical University | Wang N.,Beijing Key Laboratory of Respiratory and Pulmonary Circulation Disorders | Hu X.,Capital Medical University | Liu C.,Capital Medical University | And 7 more authors.
Canadian Journal of Cardiology | Year: 2014

Background: The diagnostic accuracy of cardiovascular magnetic resonance (CMR) for pulmonary hypertension (PH) compared with right heart catheterization were assessed. The purpose of this systematic review was to comprehensively evaluate the diagnostic accuracy of CMR in evaluating PH. Methods: Published literature was obtained from PUBMED, Web of Knowledge, Cochrane library, Embase, Biosis Preview, China National Knowledge Infrastructure, and Chongqing VIP databases, and all studies were inclusive until December 2012. Studies relevant to PH and its imaging in CMR and right heart catheterization were included if correlation coefficient was elucidated clearly. Quality Assessment of Diagnostic Accuracy Studies 2 (QUADAS-2) score was used to assess the quality of studies. Sensitivity and specificity were pooled separately and compared with overall accuracy measures: diagnostic odds ratio and symmetric summary receiver operating characteristic. Results: Sixteen studies were included in the systematic review. Of all the studies, the most widely used index was ventricular mass index (VMI) of CMR. We performed a meta-analysis for VMI among 429 patients in 5 individual studies, which showed a modest diagnostic accuracy of VMI for PH with a summary sensitivity and specificity of 84% (95% confidence interval, 79%-87%) and 82% (95% confidence interval, 73%-89%), respectively. In addition, the summary positive likelihood ratio was 4.894, indicating that VMI of CMR allows a modest ability to distinguish PH patients from healthy subjects with a cutoff point of 0.45 using functional and structural measures. Conclusions: This systematic review and meta-analysis indicates that VMI seems to have a moderate sensitivity and specificity for detection of PH. The application values of other parameters still need further investigation. © 2014 Canadian Cardiovascular Society.


Wang A.,Capital Medical University | Wang A.,Beijing Municipal Key Laboratory of Clinical Epidemiology | Liu X.,Hebei United University | Liu X.,Tangshan Peoples Hospital | And 10 more authors.
Journal of Hypertension | Year: 2014

Resting heart rate (RHR) is a predictive risk factor of the development of cardiovascular diseases, but its association with arterial hypertension has remained unclear. This study investigated the relationship between RHR and new-onset hypertension (NOH) in an Asian population. METHODS:: The Kailuan study is a prospective longitudinal cohort study on cardiovascular risk factors and cardiovascular or cerebrovascular events. Hazard ratios with 95% confidence intervals (CIs) were calculated using Cox regression modelling. RESULTS:: Out of 101 510 individuals originally included into the Kailuan study, 31 507 participants (mean age: 46.3 ± 11.5 years) were selected with no previous arterial hypertension or cardiac arrhythmias. After a mean follow-up period of 3.5 ± 0.9 years, 12 565 (39.88%) individuals developed arterial hypertension. Incidence of hypertension was 104.4, 109.7, 114.2 and 124.6 per 1000 person-years for each RHR quartile. In multivariate analysis with adjustment for blood pressure, blood lipids, diabetes mellitus and other parameters, hazard ratios for NOH increased significantly (P < 0.0001) with increasing RHR quartile. Increase in RHR by 10 beats/minute was associated with an 8% increase in NOH. Individuals in the highest RHR quartile as compared with participants in the lowest quartile demonstrated a 16% greater risk of developing NOH [hazard ratio 1.16; 95% confidence interval (CI) 1.11-1.23]. There were no significant interactions between RHR and prehypertension, diabetes mellitus, age and BMI in terms of NOH risk, respectively. CONCLUSION:: Independently of other baseline parameters such as blood pressure, blood lipids and diabetes mellitus, elevated RHR significantly increases the risk of incident hypertension. Measuring RHR is helpful in predicting the risk of eventual arterial hypertension.


Wu J.,Capital Medical University | Wu J.,Beijing Municipal Key Laboratory of Clinical Epidemiology | Liu Z.,Capital Medical University | Liu Z.,Beijing Municipal Key Laboratory of Clinical Epidemiology | And 3 more authors.
PLoS ONE | Year: 2014

Background: Adiponectin plays an important role in regulating glucose levels and fatty acid oxidation. Multiple studies have assessed the association between rs2241766 polymorphism in the adiponectin (ADIPOQ) gene and obesity susceptibility. However, the results are inconsistent and inconclusive. The aim of this meta-analysis was to investigate this association in adults. Method: Several electronic databases were searched for relevant literature published up to November 2013. Statistical analyses were performed using software Review Manager (Version 5.02) and STATA (Version 10.0). The pooled odds ratios (ORs) and 95% confidence intervals (CIs) were calculated with a random-effects model or a fixed-effect model depending on heterogeneity among studies. Q tests and Egger's tests were performed to assess heterogeneity and publication bias. Sensitivity analysis was conducted to confirm the reliability and stability of the meta-analysis. Results: A total of 2,819 obese and 3,024 controls in 18 case-control studies were included in the meta-analysis. The results indicated that compared with TT genotype, the ADIPOQ-rs2241766 GG genotype was associated with an increased risk for obesity (OR = 1.39, 95% CI: 1.11-1.73, P for heterogeneity = 0.520, I2 = 0%) in overall studies. Whereas, GT genotype was associated with a borderland increased risk for obesity (OR = 1.13, 95% CI: 0.94-1.36, P for heterogeneity = 0.006, I2 = 51%). The susceptibility of obesity was increased based on genotypes of TT


Li P.-G.,Chinese Academy of Agricultural Sciences | Mu T.-H.,Chinese Academy of Agricultural Sciences | Mu T.-H.,Capital Medical University | Deng L.,Chinese Academy of Agricultural Sciences | Deng L.,Beijing Municipal Key Laboratory of Clinical Epidemiology
World Journal of Gastroenterology | Year: 2013

AIM: To investigate the effects of proteins purified from sweet potato storage roots on human colorectal cancer cell lines. METHODS: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Hoechst 33258 nuclear staining and Boyden transwell chamber methods were used to determine whether purified sweet potato protein (SPP) from fresh sweet potato roots affected proliferation, migration and invasion, respectively, of human colorectal cancer SW480 cells in vitro. The inhibitory effects of SPP on growth of human colorectal cancer HCT-8 cells intraperitoneally xenografted in nude mice and spontaneous lung metastasis of murine Lewis lung carcinoma 3LL cells subcutaneously transplanted in C57 BL/6 mice were also investigated in vivo. RESULTS: SPP inhibited the proliferation of SW480 cells in a dose-dependent manner, with an IC50 value of 38.732 μmol/L (r2 = 0.980, P = 0.003) in the MTT assay. Hoechst 33258 nuclear staining further revealed inhibition of cell viability and induction of apoptosis by SPP. The transwell assay disclosed significant reduction in migrated cells/field by 8 μmol/L SPP (8.4 ± 2.6 vs 23.3 ± 5.4, P = 0.031) and invaded cells/field through the ECMatrix by 0.8 μmol/L SPP, compared with the control (25.2 ± 5.2 vs 34.8 ± 6.1, P = 0.038). Both intraperitoneal (ip) and intragastric (ig) administration of SPP led to significant suppression of growth of intraperitoneally inoculated HCT-8 cells in nude mice to 58.0% ± 5.9% (P = 0.037) and 43.5% ± 7.1% (P = 0.004) of the controls, respectively, after 9 d treatment. Bloody ascites additionally disappeared after ip injection of trypsin inhibitor. Notably, ig and ip administration of SPP induced a significant decrease in spontaneous pulmonary metastatic nodule formation in C57 BL/6 mice (21.0 ± 12.3 and 27.3 ± 12.7 nodules/lung vs 42.5 ± 4.5 nodules/lung in controls, respectively, P < 0.05) after 25 d treatment. Moreover, the average weight of primary tumor nodules in the hind leg of mice decreased from 8.2 ± 1.3 g/mice in the control to 6.1 ± 1.4 g/mice in the ip group (P = 0.035). CONCLUSION: SPP exerts significant antiproliferative and antimetastatic effects on human colorectal cancer cell lines, both in vitro and in vivo. © 2013 Baishideng. All rights reserved.


Yang Y.,Capital Medical University | Liang L.,Capital Medical University | Zhai Z.,Capital Medical University | He H.,Capital Medical University | And 5 more authors.
PLoS ONE | Year: 2011

Background: There has not been sufficient evidence to support the Asians being less susceptible to pulmonary embolism (PE) than other ethnicities, because the prevalence of PE/deep venous thrombosis (DVT) in different racial and ethnic groups has not been carefully studied until recently except in Caucasians. To test the hypothesis that the Chinese population has a lower risk for PE, this study comprehensively assessed the hospital-based incidence and case fatality rates for PE during the 1997-2008 in China. Methods: A registration study of patients with suspected PE syndromes admitted to 60 level-3 hospitals involved in the National Cooperative Project for the Prevention and Treatment of Venous Thromboembolism (NCPPT) was conducted from January 1997 to December 2008. The only exclusion criterion was an age of less than 18 years. Helical computed tomography scan, ventilation-perfusion lung scintigraphy or pulmonary angiography was carried out before or after hospitalization. All images were reviewed and evaluated independently by two specialists. Results: A total of 18,206 patients were confirmed with PE from 16,972,182 hospital admissions. The annual incidence was 0.1% (95% CI: 0.1% to 0.2%). The overall incidence of PE in male patients (0.2%, 95% CI: 0.1% to 0.3%) was higher than that in female patients (0.1% and 95% CI: 0.0% to 0.1%). An increasing incidence gradient for PE was noticed from Southern to Northern China. In addition, the case fatality rate was apparently decreasing: 25.1% (95% CI: 16.2% to 36.9%) in 1997 to 8.7% (95% CI: 3.5% to 15.8%) in 2008. Conclusions: Our findings suggest the relatively stable PE incidence and decreasing fatality trends in Chinese hospitals may be partially attributable to the implementation of the NCCPT and suggest the government should reevaluate the severity of PE so that health resources for the prevention, diagnosis and treatment of PE could be used to their fullest. © 2011 Yang et al.


Wu H.,Capital Medical University | Sun T.,Capital Medical University | Wang J.,Capital Medical University | Li X.,Capital Medical University | And 8 more authors.
Journal of Digital Imaging | Year: 2013

The objective of this study was to investigate the method of the combination of radiological and textural features for the differentiation of malignant from benign solitary pulmonary nodules by computed tomography. Features including 13 gray level co-occurrence matrix textural features and 12 radiological features were extracted from 2,117 CT slices, which came from 202 (116 malignant and 86 benign) patients. Lasso-type regularization to a nonlinear regression model was applied to select predictive features and a BP artificial neural network was used to build the diagnostic model. Eight radiological and two textural features were obtained after the Lasso-type regularization procedure. Twelve radiological features alone could reach an area under the ROC curve (AUC) of 0.84 in differentiating between malignant and benign lesions. The 10 selected characters improved the AUC to 0.91. The evaluation results showed that the method of selecting radiological and textural features appears to yield more effective in the distinction of malignant from benign solitary pulmonary nodules by computed tomography. © 2013 Society for Imaging Informatics in Medicine.


Lu J.,Capital Medical University | Lu J.,Beijing Municipal Key Laboratory of Clinical Epidemiology | Guo Q.,Capital Medical University | Guo Q.,Beijing Municipal Key Laboratory of Clinical Epidemiology | And 5 more authors.
Molecular Biology Reports | Year: 2012

We aimed to evaluate the contribution of the G-protein β3 subunit C825T (GNB3-C825T) polymorphism to essential hypertension (EH) in Han Chinese population by performing meta-analysis. A meta-analysis was performed in 12 case-control genetic association studies including 3,020 hypertension patients and 2,790 controls from MEDLINE (PubMed) and the China National Knowledge Infrastructure platforms. The STATA 10.0 software was used in analysis. Overall, there was no significant association between the GNB3-C825T polymorphism and EH in neither additive [TT vs. CC: OR (95 % CI) = 1.11 (0.74-1.69), P = 0.61; TC vs. CC: OR (95 % CI) = 1.08 (0.89-1.31), P = 0.42], nor dominant [TT + TC vs. CC: OR (95 % CI) = 1.11 (0.86-1.42), P = 0.43] and nor recessive [TT vs. TC + CC: OR (95 % CI) = 1.04 (0.75-1.44), P = 0.81] genetic models. Although further subgroup analysis found statistically significant results [T vs. C: OR (95 % CI) = 1.50 (1.05-2.15), P = 0.03] in the southern population, but after exclusion one particular study, the significant association was disappeared. No significant result was found in the northern Han Chinese population. There was no significant association identified between GNB3-C825T polymorphism and EH in Han Chinese population. Further larger sample and well-designed studies are needed to assess the genetic association particularly in the southern Han Chinese population. © 2012 Springer Science+Business Media B.V.


Yuan L.-H.,Capital Medical University | Yuan L.-H.,Beijing Municipal Key Laboratory of Clinical Epidemiology | Meng L.-P.,China National Institute for Nutrition and Food Safety | Ma W.-W.,Capital Medical University | And 5 more authors.
British Journal of Nutrition | Year: 2012

The correlation of glutathione S-transferase (GST) M1/T1 genetic polymorphisms with oxidative stress-related chronic diseases was proved recently. The aim of the present study was to investigate the association of GSTM1/T1 genetic polymorphisms with antioxidant biomarkers and consumption of fruits and vegetables (F&V) in healthy subjects. In this study, for conducting a 3 d dietary survey, 190 healthy adults were recruited. After DNA extraction, a multiple PCR method was used for GSTM1/T1 genotyping. A spectrophotometer method was applied for the determination of plasma total antioxidant capacity (T-AOC), vitamin C level and erythrocyte GST enzyme activity. A general linear model was used to compare the mean values of antioxidant parameters for different GSTM1/T1 genotypes and consumption of F&V. Polymorphisms of GSTM1/T1 had no effects on plasma T-AOC and vitamin C levels. Deletion of the GSTM1 gene decreased the erythrocyte GST activity. There was correlation between plasma T-AOC and consumption of F&V in the GSTM1 - or GSTT1 + subjects. A similar pattern was evident for erythrocyte GST activity in the GSTM1 - subjects. No association was found among consumption of F&V and GSTM1/T1 genotypes and plasma vitamin C level. Different consumption of F&V had no impact on plasma T-AOC and vitamin C levels in the GSTM1 -/GSTT1 + or GSTM1 -/GSTT1 - subjects. The erythrocyte GST activity was more sensitive to consumption of F&V in the individuals with the GSTM1 -/GSTT1 + genotype. Association was found among GSTM1/T1 genotypes, antioxidant parameters and consumption of F&V. Large-scale and multiple ethnic studies are needed to further evaluate the relationship. © 2011 The Authors.


PubMed | Capital Medical University and Beijing Municipal Key Laboratory of Clinical Epidemiology
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

Recently, genome-wide association studies on cardio-vascular disease identified a series of associated single nucleotide polymorphisms in an intergenic region of chromosome 1p13.3. We investigated the association of this locus with cardiovascular disease in 13 case-control studies and undertook a meta-analysis for effect size, heterogeneity, publication bias, and strength of evidence. English and Chinese language articles were screened for the association of 1p13.3 single nucleotide polymorphisms with coronary heart/artery disease or myocardial infarction as primary outcomes. The included articles provided race, numbers of participants, and the data necessary to compute an odds ratio. Articles were excluded if other outcomes were reported or 1p13.3 single nucleotide polymorphisms were not included. Thirty-five articles were initially identified and 12 were eventually included in the meta-analysis. rs599839 and rs646776, representing the 1p13.3 locus, were genotyped in 13 case-control studies involving a total of 17,766 patients and 20,272 controls. For rs599839 (11 data sets), using a random-effect model, the summary odds ratio was 1.17 (95% confidence interval = 1.07-1.28, P = 0.0001). For rs646776 (4 data sets), using a fixed-effects model, the summary odds ratio was 1.13 (95% confidence interval = 1.06-1.21, P = 0.0001). This broad replication provided unprecedented evidence for an association between genetic variants at chromosome 1p13.3 and the risk of cardiovascular disease.

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