Fan W.-H.,Beijing Military Region General Hospital |
Wang X.-Q.,Peking University
Chinese Journal of New Drugs | Year: 2012
Objective: To improve the oral bioavailability of sorafenib, a poorly water-soluble drug, self-microemulsifying drug delivery system (SMEDDS) of sorafenib was prepared and its oral relative bioavailability in rats was evaluated. Methods: The SMEDDS was prepared from a mixture of ethyl oleate, Tween-80, PEG-400 and ethanol. The plasma concentration of sorafenib after oral administration to rats was determined by HPLC. Results: The particle size of emulsified sorafenib SMEDDS was 20~25 nm. The stability studies showed little changes in particle size and drug concentrations during 8 h after the forming of the microemulsions in saline or 5% glucose solution. Compared to sorafenib suspension, the SMEDDS significantly increased the area under the concentration-time curve (AUC 0~72h), maximal blood concentration (C max) and mean residence time (MRT) values. Most importantly, the oral relative bioavailability based on AUC 0~72h increased about 25 times after associating sorafenib to SMEDDS. Conclusion: SMEDDS may be a promising vesicle for sorafenib.
Gao Y.,Chinese PLA General Hospital |
Gao Y.,Shanghai University |
Meng H.,Air Force General Hospital |
Meng H.,Beijing Military Region General Hospital |
And 9 more authors.
Human Molecular Genetics | Year: 2015
Recently, a large number of long non-coding RNAs (lncRNAs) have been reported in mammalian genomes and are evolutionarily conserved and presumably function in many biological events, especially in the pathogenesis of diverse human cancers. A lncRNA, named HOST2 (human ovarian cancer-specific transcript 2), was once reported to specifically be expressed at high level in human ovarian cancer. However, how HOST2 acts to regulate gene functions in ovarian carcinogenesis has remained enigmatic. Here we report, for the first time, that HOST2 promotes tumor cell migration, invasion and proliferation in epithelial ovarian cancer by working in key aspects of biological behaviors. In the present study, bioinformatics analysis indicated that HOST2 binds with microRNA let-7b, a potent tumor suppressor, which was then verified to target HOST2. Our results showed that HOST2 harbors a let-7b binding site and modulates let-7b availability by acting as a molecular sponge. HOST2 inhibits let-7b functions, which post-transcriptionally suppress the expression of targets, including some oncogenes that regulate cell growth and motility. Additionally, understanding HOST2/let-7b-dependent regulation may lead to alternative approaches for the diagnosis and cure of this deadly disease. © The Author 2014. Published by Oxford University Press. All rights reserved.
Lu H.-W.,Chinese People's Liberation Army |
Lu H.-W.,Xi'an Jiaotong University |
Chen Y.-B.,Beijing Military Region General Hospital |
Li Y.-M.,Xi'an Jiaotong University |
And 2 more authors.
Chinese Medical Journal | Year: 2010
Background In clinical liver transplantation, whether the delay of hepatic arterial ischaemia increases biliary fibrosis or not is controversial. We designed a liver transplantation model to test this controversy and explore its mechanism. Methods Twelve dogs were divided into two groups randomly: hepatic arterial ischaemia (HAI) and control groups. In HAI group, hepatic artery was perfused 60 minutes after portal perfusion, but in control group, hepatic arterial perfusion was simultaneous with portal perfusion. The pathological changes of intrahepatic bile ducts were observed. Transforming growth factor beta 1 (TGF-β1), expressed in epithelial cells of intrahepatic bile duct, was detected by immunohistochemical streptoadividin-biotin complex method. Expressions of Smad3, P-Smad3 and the transcriptional levels of alpha smooth muscle actin (α-SMA) mRNA in intrahepatic bile ducts were detected by Western blotting and RT-PCR respectively. Results Compared with the control group, more collagen deposition and leucocytic infiltration could be seen in biliary vessel walls. Significantly more buffy particles, which are the proteins of TGF-β1, could be seen in biliary epithelial cells. P-Smad3 and α-SMA mRNA (as ratio to corresponding β-actin) in intrahepatic bile ducts were 1.82±0.18 and 1.86±0.73 respectively in HAI group, significantly higher than those in control group (0.59±0.09 and 0.46±0.18, respectively). Conclusions Hepatic arterial ischaemia could increase the deposition of collagen fibres, trigger the transdifferentiation of myofibroblasts in intrahepatic bile duct and might result in biliary fibrosis by activating the TGF-β1 signalling pathway.
Wei Z.-J.,Beijing Military Region General Hospital |
Ou Y.-Q.,Beijing Military Region General Hospital |
Li X.,Beijing Military Region General Hospital |
Li H.,Beijing Military Region General Hospital
Neurological Sciences | Year: 2014
The aim of the study was to assess the 90-day prognostic value of copeptin in a group of Chinese patients with acute intracerebral hemorrhage (ICH). In this study, all consecutive patients with first-ever ICH from 2010 to 2012 were recruited to participate in the study. On admission, plasma copeptin levels were measured by enzyme-linked immunosorbent assay. The Glasgow Coma Scale (GCS) and Hemphill ICH scores were assessed on admission blinded to plasma copeptin levels. For the assessment of functional outcome at 90 days, Modified Rankin Scale was used. During the study period, 271 patients were diagnosed as ICH and were included in the analysis. The median GCS score on admission was 11 points. Patients with an unfavorable outcomes and non-survivors had significantly increased plasma copeptin levels on admission (P < 0.001 for both). Copeptin was an independent prognostic marker of functional outcome and death [odds ratio 3.45 (95 % confidence intervals: 1.85–6.99) and 3.66 (2.42–8.28), respectively, P < 0.001 for both, adjusted for age, the hematoma volume and other predictors] in patients with ICH. In receiver operating characteristic curve analysis, copeptin could improve the Hemphill score in predicting 90-day functional outcome [area under the curve (AUC) of the combined model, 0.83; 95 % CI 0.74–0.90; P < 0.001] and mortality (AUC of the combined model, 0.88; 95 % CI 0.82–0.93; P < 0.001). In conclusion, our study suggests that copeptin levels are a useful tool to predict unfavorable functional outcome and mortality 90 days after ICH and have a potential to assist clinicians. © 2014, Springer-Verlag Italia.
Zhu L.,Chongqing Medical University |
Zhu L.,Chinese People's Liberation Army |
Zhang H.,Beijing Military Region General Hospital |
Shi F.,Chinese People's Liberation Army |
And 2 more authors.
Burns | Year: 2015
Objectives: The objective of this study was to investigate the epidemiological characteristics of scalds induced by "guo lian kang" (literally "stove connected to bed") in children. Methods: The demographic, burn features, outcome, and other information of 0-14-year-old children with "guo lian kang"-induced scalds admitted to the burn treatment center of the 322 Hospital of the People's Liberation Army in Datong from 30 September 2001 to 31 December 2011 were analyzed. Results: Among the 1993 children clearly documented with scalds due to hot liquid in vessels, 795 (39.89%) had burns due to "guo lian kang." The proportion of the pediatric burns caused by "guo lian kang" was 55.56% in 2002 and 37.30% in 2011, with a decreasing trend. One-year-old children accounted for the highest proportion of children with burns due to "guo lian kang" (male:female = 1.54:1). Scalding mostly occurred from October to May; the most common times were around 8:00 AM, 11:00 AM, and 7:00 PM. Most scalds occurred on the upper limbs, 90.22% of which were dermal burns. The median burn area was 11% of the total body surface area. The burn agent was mainly hot water in pots. Scalding mostly occurred when the children accidentally sat on, or fell or stepped into the pot while playing. Among the children with "guo lian kang"-induced scalds, up to 42.26% of the children did not receive any treatment before hospital admission, and 18.86% of the children were admitted to the hospital ≥6 h after the occurrence of burns. In addition, 74.97% of the children's families can afford the medical expenses, while 5.28% abandoned treatment because of financial difficulties. There were no significant differences between children with scalds caused by "guo lian kang" and scalds caused due to other reasons considering the age and sex. Compared to scalds due to other reasons, children with "guo lian kang"-induced scalds had greater burn area and depth, shock incidence, surgery rate, blood transfusion rate, length of hospital stay, and hospitalization costs. Conclusion: Preventive measures against burns should be taken from October to May and mainly during cooking time in order to prevent burns in children less than 3 years of age, especially in 1-year-old boys. The most simple and effective preventive measure is to install a barrier between the pot and bed. The incidence of shock in children may be reduced if parents and primary medical staff undergo training to perform first aid for burns and if there are improvements in the referral system after burns. © 2014 Elsevier Ltd and ISBI. All rights reserved.
Wang D.-W.,Beijing Military Region General Hospital |
Zhang Y.,Beijing Military Region General Hospital |
Yao J.-M.,Beijing Military Region General Hospital |
Xiao Z.-B.,Beijing Military Region General Hospital
Experimental and Therapeutic Medicine | Year: 2013
Essential thrombocythemia (ET) is a chronic clonal myeloproliferative disorder, which is often complicated by arterial or venous thrombosis and idiopathic bleeding diathesis. The present study reports a female patient with ET complicated by acute myocardial infarction, leading to ventricular aneurysm following interventional therapy for 3 years and a subsequent in-stent restenosis. Following careful examination, a ventricular aneurysm resection and coronary artery bypass graft were carried out. During this case, the monitoring and controlling of the platelet count, pre- and post-operatively, was extremely important for successful surgery.
Guan Y.,Beijing Military Region General Hospital |
Song H.,The 309Th Hospital of Chinese PLA |
Zhang G.,Beijing Military Region General Hospital |
Ai X.,Beijing Military Region General Hospital
Oncology Reports | Year: 2014
Flotillin-1 (FLOT1) is known to have a role in tumorigenesis; however, the effect of FLOT1 on proliferation and recurrence of human transitional cell carcinoma (TCC) is unclear. Samples from 156 TCC patients and 142 patients undergoing open bladder surgery for indications other than TCC were used in the present study. FLOT1 protein expression was determined by immunohistochemistry and western blot analysis, and mRNA expression was detected by RT-PCR and real-time PCR. A FLOT1-expressing pcDEF3 vector was stably transfected into 4 TCC cell lines and FLOT1 expression was decreased by RNAi. Proliferative analysis of TCC cells was detected by the WST-1 assay and a xenograft model using BALB/C nude mice. The association between FLOT1 expression and TCC recurrence was also analyzed by adhesion, migration and invasion assays. FLOT1 expression in TCC was significantly overexpressed compared to normal urothelial tissue, and the level of FLOT1 expression was significantly correlated with tumor size, pathologic grade, clinical stage and recurrence. In addition, FLOT1 significantly increased the proliferative ability of TCC cells in vitro and in vivo. TCC cells with a high level of FLOT1 expression exhibited a higher level of adhesion, migration and invasion. FLOT1 expression was shown to be upregulated in human TCC. These findings suggest that FLOT1 plays an important role in the proliferation and recurrence of TCC and that silencing FLOT1 expression might be a novel therapeutic strategy.
Wang D.,Beijing Military Region General Hospital |
Yao J.,Beijing Military Region General Hospital |
Zhao X.,Beijing Military Region General Hospital |
Lu L.,Beijing Military Region General Hospital |
Cui Z.,Beijing Military Region General Hospital
International Journal of Clinical and Experimental Medicine | Year: 2016
Coronary atherosclerotic heart disease (CHD) is the most common type of cardiovascular disease with increased incidence and serious damage to human life and health. Left main coronary artery lesions lead to severe ischemic symptoms, including ventricular fibrillation, cardiac shock, cardiac arrest, and even death. At present, two kinds of intervention such as percutaneous coronary intervention (PCI) and coronary artery bypass graft (CABG) could be used for treatment. Comparison of their effects is still under investigation. 462 cases of patients who were diagnosed as left main coronary artery disease and received blood reconstruction surgery in our hospital between Jan 2009 and Dec 2012 were retrospectively analyzed. 270 cases were treated by PCI combined drug-coated stents, and 192 cases received CABG therapy. Parsonnet score and Karnofsky score were compared. Target lesion revascularization rate, incidence of myocardial infarction, adverse cerebrovascular events (MACCE), mortality, and curative effect were evaluated. No statistical difference was observed in Parsonnet score (P > 0.05). Follow-up analysis found PCI group has higher rate of target lesion revascularization, incidence of angina pectoris, incidence of MACCE than CABG group, whereas lower Karnofsky score (P < 0.05). Myocardial infarction and fatality rate were similar between two groups. In conclusion, our study showed that PCI and CABG showed similar curative effect for the treatment of left main coronary artery lesions, while CABG presented better long-term curative effect. © 2016, E-Century Publishing Corporation. All Rights Reserved.
PubMed | Chinese PLA General Hospital, Massachusetts General Hospital and Beijing Military Region General Hospital
Type: Journal Article | Journal: Journal of clinical pharmacology | Year: 2016
The authors performed a meta-analysis to compare the characteristics of clonidine and dexmedetomidine as adjuvants to local anesthetic in intravertebral anesthesia. Four investigators independently searched electronic databases for randomized trials comparing the characteristics of clonidine vs dexmedetomidine as adjuvants to local anesthetic on adults. The endpoints were onset of analgesia, sensory and motor block, and duration of analgesia. A random-effects model was used to perform quantitative analysis. Seven studies comprising 354 subjects were included in this meta-analysis. The onset of sensory block was significantly 40 seconds shorter when dexmedetomidine was added as an adjuvant in the intrathecal injection. The duration of stable sensory block, duration of overall sensory block, and the time before the need for analgesic requirements were significantly extended, 10.8 minutes, 22.3 minutes, and 38.6 minutes, respectively, when dexmedetomidine was used as an adjuvant to local anesthetics (bupivacaine or ropivacaine). No significant differences were detected in the motor block characteristics and the time to achieve peak sensory level between dexmedetomidine and clonidine as adjuvants to local anesthetics. Compared to clonidine, the addition of dexmedetomidine as an adjuvant to local anesthetics is associated with earlier, prolonged sensory block characteristics and later need for analgesic requirements.
PubMed | Chongqing Medical University, Chinese People's Liberation Army and Beijing Military Region General Hospital
Type: Journal Article | Journal: Burns : journal of the International Society for Burn Injuries | Year: 2014
Retrospective surveys of all hospitalized pediatric burns under the age of 15 years were conducted in 18 hospitals from 5 provinces and municipal cities of North China between 2001 and 2010. A total of 17,770 patients were included in this study. The epidemiological characteristics of hospitalized pediatric burns and influencing factors of length of hospital stay and hospitalization cost were analyzed. In this study, children accounted for 43.57% of all hospitalized burns, with a gradually increasing trend (P=0.003). Among children hospitalized burns, the percentage of children younger than three years was 69.9%, with an upward trend (P<0.001). The ratio of male to female was 1.53:1. Scald burns accounted for 89.79% and 71.54% had burns of <10% total body surface area, with increasing trends (P0.001). The medians of length of hospital stay and hospitalization cost were eight days and 2469 RMB yuan respectively. The most important factors affecting length of hospital stay and hospitalization cost were burned surface area, surgery and treatment outcome. Children under three years of age, boys and children with a small area of mild scald burns should be made the focus of childhood burn prevention. Improving the medical insurance system for children is urgently needed.