Beijing Laboratory Animal Research Center

Beijing, China

Beijing Laboratory Animal Research Center

Beijing, China
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Gao J.,China Agricultural University | Hou R.-G.,China Agricultural University | Zhang H.-Q.,China Agricultural University | He J.-Z.,China Agricultural University | And 3 more authors.
Letters in Applied Microbiology | Year: 2011

Aims: To develop a PCR-based assay to detect Prototheca zopfii (P. zopfii) and its mastitis-related subtype (genotype 2) directly from milk samples. Methods and Results: The DNA extraction method herein is based on the lysing properties of chemical agents, mechanical grinding and DNA-binding properties of silica particles; this method was developed to rapidly extract DNA directly from P. zopfii in bovine milk. Two pairs of primers specific for P. zopfii and genotype 2 were used in the duplex PCR, and a sensitivity test showed that the detection level was 5×10 2 colony-forming units (CFU)ml -1 for P. zopfii and 5×10 3CFUml -1 for genotype 2. Furthermore, a practical survey of 23 milk samples showed that the assay produced results that were in accordance with those obtained by the conventional microbiology method. Conclusions: The DNA extraction method is effective in isolating sufficient quantities of DNA from P. zopfii in milk for PCR analysis. The PCR assay is economical, sensitive and more rapid than the conventional culture method. Significance and Impact of the Study: The assay could be used as an alternative method for the rapid the detection of bovine mastitis resulting from P. zopfii genotype 2. © 2011 The Authors. Letters in Applied Microbiology © 2011 The Society for Applied Microbiology.

Liu Y.,Centers for Disease Control and Prevention | Wang L.,Beijing Laboratory Animal Research Center | Wang Z.-J.,Centers for Disease Control and Prevention
Asian Pacific Journal of Cancer Prevention | Year: 2014

ELDF15, homologous with AT2 receptor-interaction protein 1 (ATIP1), may play an important role in cell differentiation, proliferation, and carcinogenesis. We aimed to understand the biological function of ELDF15 via construction and transfection of a recombinant expression vector containing antisense ELDF15. Recombinant expression vectors were successfully constructed and transfected into K562 cells. A stable transfectant, known as pXJ41-asELDF15, stably produced antisense ELDF15. Compared with K562 and K562-zeo cells, K562- pXJ41-asELDF15 cells showed inhibition of cell proliferation. RT-PCR analysis showed that the expression and protein level of ELDF15 decreased significantly in K562 cells transfected with pXJ41-asELDF15. Expression of hemoglobin increased in K562 cells transfected with pXJ41-asELDF15 by benzidine staining. increases NBT reduction activity in K562 cells transfected with pXJ41-asELDF15.Colony forming efficiency in two-layer soft agar was clearly inhibited as assessed by electron microscopy. These results suggest that ELDF15 plays a potential role in cell differentiation, proliferation and carcinogenesis.

Ou C.,China Agricultural University | Pu Z.,Mianyang Normal University | Li S.,Beijing Laboratory Animal Research Center | Pan Q.,China Agricultural University | And 3 more authors.
Journal of Animal and Veterinary Advances | Year: 2011

4-hydroxycinnamic acid was firstly isolated from Fructus mume and identified to be one of the main components. The studies aimed to investigate the immunotherapeutic potential of 4-hydroxycinnamic acid on SPF chickens infected with IBDV The results showed that the bird survival rate of 3 dosage groups of 4-hydroxycniuaruic acid was 87.5, 62.5 and 42.86%, respectively while body weight gain rate was 25.68, 25.28 and 21.37%, respectively. As for the survival rate and body weight gain, no significant difference was found between 35 mg kg-1 dosage and high immune yolk antibody group (p>0.05). The histopathology score of the high dosage group 3 was lower than that of the high immune yolk antibody group 6. The Stimulate Index (SI) of spleen lymphocytes of chickens fed 4-hydroxycinnamic acid (SI = 1.69) was significantly higher than that of untreated chickens (SI = 1.36) (p<0.05). These results showed that chickens fed with 35 mg kg-1 4-hydroxycinnamic acid for 5 days could effectively induce a high protection against IBDV and its related mechanism may enhance cell immunity. © Medwell Journals, 2011.

Gao J.,China Agricultural University | Yu F.-Q.,National Animal Husbandry | Luo L.-P.,China Agricultural University | He J.-Z.,China Agricultural University | And 5 more authors.
Veterinary Journal | Year: 2012

The aim of this study was to characterise the phenotypic and genotypic antibiotic resistance patterns of Streptococcus agalactiae isolated from cows with mastitis in China. Antibiotic resistance was based on minimum inhibitory concentrations and detection of resistance genes by PCR. S. agalactiae isolates most frequently exhibited phenotypic resistance to tetracycline, while the resistance genes most frequently detected were ermB, tetL and tetM. Resistance genes were detected in some susceptible isolates, whereas no resistance genes could be detected in some resistant isolates, indicating that the resistance genotype does not accurately predict phenotypic resistance. © 2012 Elsevier Ltd.

Gao J.,China Agricultural University | Zhang H.,China Agricultural University | He J.,China Agricultural University | He Y.,China Agricultural University | And 5 more authors.
Mycopathologia | Year: 2012

Prototheca zopfii (P. zopfii) has become an important cause of bovine mastitis in many countries. In the present study, to better understand the occurrence of one clinical mastitis (CM) outbreak due to P. zopfii, the molecular characterization and resistance patterns of the microalga were described. P. zopfii strains were isolated from 17 of 23 quarters, which suffered CM in the outbreak, and 7 of 46 CM recovered quarters before the outbreak, as well as 2 of 75 environmental samples in the dairy farm. All strains were identified as genotype 2 by genotype-specific PCR analysis. Results of in vitro antimicrobial and antifungal susceptibility tests indicated that these strains were resistant to majority of tested drugs, with the only exception of amphotericin B, nystatin, streptomycin, gentamicin, and amikacin. This is the first report about CM outbreak caused by P. zopfii in China. These data suggest that P. zopfii may represent a serious risk in the studied herd, and this microalga could be an important potential pathogen causing mastitis in dairy herds of Beijing. © 2011 Springer Science+Business Media B.V.

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