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Zhu C.,Beijing Key Laboratory of Transplantation and Immune Regulation
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2013

To identify specific antigens related to streptomycin resistant (SMr) Mycobacterium tuberculosis. Cellular proteins were extracted from SMr clinical isolate 01108, SM-sensitive clinical isolate 01105 and H37Rv. Differential expression proteins were identified with isobaric tags for relative and absolute quantitation (iTRAQ) combined with Nano LC-MS/MS technology. Approximately 194 and 146 differential expression proteins were identified in 01108 strain compared with the proteomic profiles of 01105 strain and H37Rv, respectively, and 121 proteins were identified in 01108 strain compared with the proteomic profiles of both 01105 strain and H37Rv. Identified proteins showed a pI (isoelectric point) variation between 3.74-12.48 and a molecular mass (M) range between 7.63 and 326.2 kDa. Differential expression proteins were mainly associated with metabolism (involved in intermediary metabolism, respiration, and lipid metabolism) and took part in catalysis and binding function. Seven ribosomal proteins (Rv0056, Rv0641, Rv0652, Rv0701, Rv1630, Rv2442c and Rv2785c) and seven proteins (the ratios > 1.20 or < 0.55) were commonly down-regulated in 01108 strain compared with both 01105 strain and H37Rv, i. e. the thiol peroxidase (Rv1932), acyl carrier protein dehydrogenase (Rv0824c), 30S ribosomal protein S15 (Rv2785c), acetone acid dehydrogenase E2 part (Rv2215), two-component transcriptional regulatory protein (Rv3133c) and Hypothetical protein (Rv2466e and Rv2626c). Differential expression proteins were found in SMr strain compared with both SM-sensitive strain and H37Rv. Further studies are needed to assess the role of these differential expression proteins in SM resistance.


Huang X.-Y.,Beijing Key Laboratory of Transplantation and Immune Regulation | Zhu C.-Z.,Beijing Key Laboratory of Transplantation and Immune Regulation | Pang Y.,National Center for Tuberculosis Control and Prevention | Shao J.-S.,Beijing Key Laboratory of Transplantation and Immune Regulation | And 4 more authors.
Medical Journal of Chinese People's Liberation Army | Year: 2014

Objective: To identify the proteins related to ofloxacin (OFX) resistance of Mycobacterium tuberculosis (MTB).Methods: Standard MTB H37Rv strain, clinical isolates of OFX resistant strain (OFXR) and sensitive strain (OFXS) were obtained from the Chinese Center for Disease Control and Prevention, and they were cultured in Sauton's medium, and then inactivated by 60Co. Whole cellular proteins were extracted from OFXR, OFXS and H37Rv strain of MTB, respectively. The peptides were labeled, separated and identified by isobaric tags of relative and absolute quantitation (iTRAQ) combined with Nano LCMS/ MS technology.Results: One hundred and seventy-five and 134 differential expression proteins were identified in MTB OFXR compared with MTB OFXSand H37Rv, respectively. One hundred and four common differential expression proteins were identified in MTB OFXR compared with both MTB OFXS and H37Rv. The isoelectric point and theoretic relative molecular mass of differential expression proteins were widely distributed. The majority of the common differential expression proteins were involved in intermediary metabolism, respiration, and lipid metabolism. Twelve common differential expression proteins showed significant differences (the ratio>1.2 or <0.55) in MTB OFXR, including Rv0106, Rv0895, Rv2185c, Rv3248c and Rv3841 up-regulation and Rv2524c, Rv2986c, Rv3118 and Rv3597c down-regulation.Conclusion: iTRAQ has been used to identify the common differential expression proteins in MTB OFXR compared with both MTB OFXS and H37Rv, which provides a basis for further study of the mechanism of OFX-resistance. © 2014, People's Military Medical Press. All rights reserved.

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