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Guo Y.,Sourthern Medical University | Guo Y.,Beijing Key Laboratory of Pediatric Organ Failure | Guo Y.,Inner Mongolia University | Chai Q.,Inner Mongolia Peoples Hospital | And 5 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

Autoimmune disease systemic lupus erythematosus (SLE) is associated with increased expression of pro-inflammatory cytokines such as interferons (IFNs) and specific interleukins (ILs), which are induced by toll-like receptors (TLRs). The present study aimed to examine the serum levels of cytokines, the activation of TLR-7 and TLR-8 of peripheral blood mononuclear cells (PBMCs) from pediatric SLE patients, and to investigate the response of those PBMCs to viral RNA via the TLR-7 and TLR-8 signaling. Results demonstrated that pediatric SLE patients had increased serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, and IFN-α, and promoted activation of TLR-7 and TLR-8, compared to control subjects. Moreover, the peripheral blood mononuclear cells (PBMCs) from pediatric SLE patients were more sensitive to the stimulation by the transfection with viral RNA from influenza virus, with a promoted activation of TLR-7 and TLR-8 signaling. In conclusion, pro-inflammatory cytokines, such as IL-1β, IL-6, IL-8, IL-10, and IFN-α were promoted in pediatric SLE patients, with an increased activation of TLR-7 and TLR- 8 to the stimuli, such as virus infection. It implies the TLR-7 and TLR-8 activation by virus infection might play an important role in the pathogenesis of pediatric SLE. © 2015 E-Century Publishing Corporation. All rights reserved.


Hong X.,Affiliated Bayi Childrens Hospital | Hong X.,Beijing Key Laboratory of Pediatric Organ Failure | Liu C.,Affiliated Bayi Childrens Hospital | Liu C.,Beijing Key Laboratory of Pediatric Organ Failure | And 14 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2016

Objective: Increasing congenital heart disease (CHD) neonates receive cardiac surgery especially after two-child policy in China. This study was to investigate the risk factors for the mortality of CHD neonates after cardiopulmonary bypass (CPB). Methods: CHD neonates (n=123) undergoing CPB were prospectively enrolled. According to the early outcome, neonates were divided into survivor group and non-survivor group. The demographics and peri-operative factors were collected and compared between them. Variables with significant difference in univariate analysis were screened out by forward likelihood selection procedure, and included for multivariable logistic regression analysis. Results: A total of 23 neonatesdied after CPB. The overall in-hospital mortality was 18.70%. Univariate analysis indicated that the RACHS-1 score (P=0.029), time of CPB (P=0.003), incidence of cardiac arrest (CA) (P=0.005), proportion of neonates receiving extracorporeal membrane oxygenation (ECMO) (P=0.040) and lactate level (P=0.003) were significantly different between two groups. These 5 variables with significant difference in univariate analysis were screened out by forward likelihood selection procedure and multivariatelogistic regression analysis was performed. Results showed the odds ratio (OR) of death was increased by 1.009 for every unit increase in the time of CPB (OR: 1.009, P=0.046) and by 4.937 for CA present postoperatively (OR: 4.937, P=0.009). Conclusion: Long CPB and postoperative presence of CA were independent risk factors for mortality in neonates after CPB. Thus, clinicians should shorten the time of CPB and take measures to reduce CA, aiming to reduce the post-operative mortality. © 2016, E-Century Publishing Corporation. All rights reserved.

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