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Shi X.,CAS Institute of Chemistry | Sun L.,Beijing Institute of Traumatology and Orthopedics | Gan Z.,CAS Institute of Chemistry
Acta Polymerica Sinica | Year: 2011

Porous polylactide (PLA) microspheres were prepared by means of the modified double emulsion solvent evaporation. The organic solvents with different boiling points and aqueous solubility were used to prepare PLA microspheres with different porous structures. Microspheres prepared with methylene chloride, chloroform and toluene as solvents had the similar homogeneous porous structure, while those microspheres with ethyl acetate as solvent showed a hollow structure with a porous thin shell. The effects of solvent types on porous structure of microspheres were discussed. It was concluded that the removal rate of solvent plays the decisive role in determining the porous structure of microspheres. Accordingly, a formation mechanism of porous microspheres fabricated with different solvents was proposed.

Cheng X.-G.,Beijing Jishuitan Hospital | Li K.,Beijing Jishuitan Hospital | Ou S.-X.,General Hospital of Guangzhou Military Command | Tang G.-Y.,Tongji University | And 4 more authors.
Journal of Clinical Densitometry | Year: 2016

This study compares spinal volumetric bone mineral density (vBMD) with spinal areal bone mineral density (aBMD) among young adults from 3 eastern provincial capital cities in Mainland China. A total of 416 young adults (age range: 20-40 yr) from 3 eastern provincial capital cities (Beijing, Shanghai, and Guangzhou) in Mainland China were recruited in this study. From each subject, the vBMD of the lumbar spine was measured by the Mindways quantitative computed tomography system. Moreover, the aBMD of the lumbar spine, measured by the dual-energy X-ray absorptiometry, was extracted from a previous multicenter large-scale study, and the 420 participants were matched by age, gender, height, weight, as well as geographic territory. The vBMD and the aBMD values were further compared and analyzed. Generally, the bone mineral density (BMD) results were significantly different among participants from the 3 cities (p <0.05). Specifically, both vBMD and aBMD values of participants from Beijing were significantly different from those from Guangzhou (p <0.05). Additionally, a statistically significant difference in aBMD values was also found between participants from Beijing and Shanghai (p <0.05). However, no significant differences were found between participants from Shanghai and Guangzhou in terms of the aBMD and vBMD values (p 1 > 0.05 and p 2 > 0.05). Interestingly, the overall mean vBMD value was 5.9% greater in women than those in men for all the 3 cities (p <0.001). This study demonstrated an overall heterogeneity in spinal BMD among young adults from 3 eastern provincial capital cities in Mainland China. Specifically, the taller and heavier young adults from the northern part of China have smaller spinal vBMD but higher spinal aBMD values than those who were shorter and lighter from the southern part of China. © 2016 International Society for Clinical Densitometry.

Wang Q.,Beijing Institute of Traumatology and Orthopedics
Wei sheng yan jiu = Journal of hygiene research | Year: 2011

To evaluate the effects of different doses of phytoestrogen (genitein) combined with calcium and vitamin D3 on preventing osteoporosis in ovariectomized (OVX) mice. 63 female CD-1 mice, 29g average weight, were randomly divided into 7 groups. Including Sham group, OVX group and groups of treatment with calcium, vitamin D3 and genistein in high dose (GH, 67 mg/kg), genistein in moderate does (GM, 33.5 mg/kg), genistein in low dose(GL,16.75 mg/kg), pure genistein (G) and pure 17-betaestradiol (E2). After six weeks treatment, bone mineral density (BMD), bone mineral content (BMC), Biomechanical characteristics bone strength and bone biochemical markers were measured in all mice. In the groups of GH, GM and GL, it was stimulative effect of genistein on elevating uterine weight in ovariectomized mice and the effect in GL group is lower. BMD, BMC, length and width of femora were significantly increased in GM group mice than those in OVX mice (P < 0.01), as well as BMD of femora in GL group mice were markedly increased (P < 0.01). Peak load and resilience of femora were the most conspicuously increased in GL group mice than those in others treatment group mice (P < 0.01). In GL group, bone alkaline phosphatase (BALP) increased and tartrate-resistant acid phosphatase (TRAP) decreased (P < 0.01). It was lower stimulative effect of low dose of phytoestrogen (genitein) combined with calcium and vitamin D3 on elevating uterine weight in ovariectomized mice. Low dose of Phytoestrogen (genistein) combined with calcium and vitamin D3 might increase BMD, improve the mechanical strength of bone, promote bone fromation and inhibit bone resorption significantly. It might reduce the dose of genistein administrated and be safter than E2.

Wang N.,Beijing Institute of Traumatology and Orthopedics | Zhao D.-H.,Beijing Institute of Traumatology and Orthopedics | Wu C.-A.,Beijing Institute of Traumatology and Orthopedics | Tian W.,Ji Shui Tan Hospital | Xiao B.,Ji Shui Tan Hospital
National Medical Journal of China | Year: 2010

Objective: To acquire information on the IVD (intervertebral disc) proteome and analyze the differences of identified proteins during IVD development and maturation by a shotgun proteomics approach so as to identify the global protein expression patterns of IVD tissues from fetus and adults. Methods: A 24-week fetus, a 25- and a 30-year-old adult IVD samples were collected and SDS-PAGE, RP-HPLC MS/MS shotgun analyses were performed. Bioinformational analysis with International Protein Index (IPI) database and functional classification with Gene Ontology Annotation (GOA) database were used to evaluate the results. Results: A total of 524 proteins were identified in fetal IVD sample while 181 and 172 proteins were observed in 25 and 30-year-old samples respectively. Forty-eight proteins existed in three samples while 84 proteins in the 25-years-old and 30-years-old samples but not in fetus. Only 174 high-quality proteins existed in fetal sample while 20 high-quality proteins in 25-year-old and 30-year-old samples. The physico-chemical characteristics of identified proteins displayed similar trends in three samples. Conclusions: This study represents the first presentation of a global proteomic map of fetal and adult IVD samples using shotgun technology. Substantial differences exist in number and variety of proteins between development and mature IVD. This contributes to our overall knowledge in of biochemical components, metabolic regulation and biological mechanics in IVD.

Qi H.,Beijing Institute of Traumatology and Orthopedics | Sun L.,Beijing Institute of Traumatology and Orthopedics | Chen L.,Beijing Institute of Traumatology and Orthopedics | Tao J.-F.,Beijing Institute of Traumatology and Orthopedics | Jiang J.,Beijing Institute of Traumatology and Orthopedics
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010

BACKGROUND: Cartilage defects can be treated with the method of tissue engineering, and the scaffold is of great importance in carrying vector and well fixation of chondrocytes. OBJECTIVE: To investigate the feasibility and validity of using complex material of biological fibrin glue and demineralized bone matrix as a scaffold for cartilage defects repair in experimental rabbits. METHODS: Scaffold was prepared with complex material of biological fibrin glue and demineralized bone matrix. Rabbit articular cartilage defect model was prepared and numbered, 42 New Zealand white rabbits were randomly divided into three groups: scaffold complex cell group (n=15): Rabbit articular cartilage defects were repaired with composite scaffold of biological fibrin glue and demineralized bone matrix seeded on the chondrocytes; single scaffold group (n=15): the defects were filled with complex scaffold without cells; blank control group (n=12): no any implant treatment was given. According to the grouping, the specimens were drawn at 4, 8, 12 weeks after surgery, and underwent gross, histological, toluidine blue staining, as well as Wakitani scores. The repair of articular defects was observed in each group. RESULTS AND CONCLUSION: Implantation of scaffold with chondrocytes had the significant effect on the cartilage defects after 12 weeks, and hyaline cartilage was the predominant, the repair effect was superior to single scaffold and blank control groups. Wakitani score showed the best repairing results in the scaffold complex cell group (P < 0.05). Complex material of biological fibrin glue and demineralized bone matrix can be used as one of the optimal scaffolds for cartilage tissue engineering and it contributes to regeneration and repair of the cartilage defects.

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