Beijing Institute of Otorhinolaryngology

Beijing, China

Beijing Institute of Otorhinolaryngology

Beijing, China
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Zhang Y.,Beijing Institute of Otorhinolaryngology | Zhang Y.,Capital Medical University | Bosse Y.,Laval University | Castano R.,University of Montréal | Desrosiers M.,McGill University
American Journal of Rhinology and Allergy | Year: 2011

Background: Nitric oxide (NO), is a biological messenger molecule and a component of innate immunity, with important roles in the regulation of inflammation and in defense against bacterial biofilms. Polymorphisms in genes regulating NO production have the potential for a role in the development of chronic rhinosinusitis (CRS). The purpose of this study was to determine whether polymorphisms in genes regulating NO synthesis are associated with CRS. Methods: An established population of 206 individuals with severe CRS and 196 postal code-matched controls was previously screened using a pooling genome-wide associations study to estimate allelic frequency. Genes regulating NO synthesis with a maximal probability of association were identified. High-probability single nucleotide polymorphisms SNPs from the NO synthase (NOS1) and its ligand NOS1 adaptor protein (NOS1AP) genes were retained for individual genotyping. PLINK software was used to determine association. Results: Sixteen SNPs were genotyped successfully with a genotype distribution in agreement with Hardy-Weinberg equilibrium. Two SNPs for NOS1 (rs1483757 and rs9658281) were significantly associated with CRS, with a protective effect. The severe subphenotype showed stronger associations. Subgroup analysis for the presence of nasal polyps, origin, and gender did not influence strength of associations. Conclusion: These data suggest that polymorphisms in the NOS1 gene may play a role in the susceptibility to develop CRS. Study findings apply to patients with severe CRS, unresponsive to surgery. Copyright © 2011, OceanSide Publications, Inc.


Wei X.,Capital Medical University | Wei X.,Beijing Institute of Otorhinolaryngology | Wei X.,Peoples Hospital of Hainan Province | Zhang Y.,Capital Medical University | And 4 more authors.
PLoS ONE | Year: 2013

Background: Allergic rhinitis (AR) is a chronic inflammatory disease of the nasal mucosa, involving a complex interaction between genetic and environmental factors. Evidence suggests that polymorphisms in the gene coding for mitochondrial ribosomal protein L4 (MRPL4), located in close proximity to intercellular adhesion molecule-1 (ICAM-1) gene on chromosome location 19p13.2, may influence the risk factor for the development of AR. Objective: The aim of our study was to investigate any association between AR susceptibility and polymorphisms in ICAM-1 gene, as well as associations between AR risk and polymorphisms in MRPL4, nuclear factor-kappaB (NF-κB) and tumor necrosis factor alpha(TNF-α) genes, associated with ICAM-1 expression. Methods: A cohort of 414 patients with AR and 293 healthy controls was enrolled from the Han Chinese population in Beijing, China. Blood was drawn for DNA extraction and total serum immunoglobulin E (IgE). A total of 14 single nucleotide polymorphisms (SNPs) in ICAM-1, NF-κB, TNF-α, and MRPL4 genes were selected using the CHB genotyping data from the International Haplotype Mapping (HapMap) and assessed for differences in frequencies of the alleles and genotypes between the AR patients and control subjects. Results: TNF-α SNP rs1799964 and MRPL4 SNP rs11668618 were found to occur in significantly greater frequencies in the AR group compared to control group. There were no significant associations between SNPs in NF-κB, ICAM-1 and AR. The SNP-SNP interaction information analysis further indicated that there were no synergistic effects among the selected sets of polymorphisms. Conclusions: Our results suggest a strong association between AR risk and polymorphisms of MRPL4 and TNF-α genes in Han Chinese population. © 2013 Wei et al.


Zhang Y.,Capital Medical University | Zhang Y.,Beijing Institute of Otorhinolaryngology | Song X.,Capital Medical University | Song X.,Beijing Institute of Otorhinolaryngology | And 5 more authors.
BMC Medical Genetics | Year: 2012

Background: Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine, implicated in the development and progression of allergic diseases. Recent studies have demonstrated significantly increased expression and synthesis of TSLPin nasal mucosa of patients with allergic rhinitis (AR), compared with nonallergic control subjects. Also, there is significant correlation between the level of TSLP mRNA and symptom severity in AR patients. In this study, we investigated whether polymorphisms in the TSLP gene were associated with increased risk of AR in the Chinese population.Methods: In a candidate gene association study, we tested 11 single nucleotide polymorphisms (SNPs) in the TSLP gene in 368 AR and 325 control adult Han Chinese subjects from Beijing. The 11 SNPs were selected from the Chinese HapMap genotyping dataset to ensure complete genetic coverage. AR was established by questionnaire and clinical examination, and blood was drawn from all subjects for DNA extraction. The PLINK software package was used to perform statistical testing.Results: In the single-locus analysis of AR risk, no significant differences in allele and genotype frequencies were found between AR and control subjects. Further logistic regression analyses adjusted for age and gender also failed to reveal significant associations between AR and the selected SNPs. Similarly, analysis stratified by gender, and haplotype or diplotype did not reveal any association with AR risk.Conclusion: Although TSLP presents itself as a good candidate for contributing to allergy, this study failed to find an association between specific SNPs in the TSLP gene and AR susceptibility in the Han Chinese population. © 2012 Zhang et al.; licensee BioMed Central Ltd.


Wang S.,Macquarie University | Wang S.,Beijing Institute of Otorhinolaryngology | Wang S.,ing Cooperative Research Center | Mannell R.,Macquarie University | And 5 more authors.
Ear and Hearing | Year: 2011

OBJECTIVE: The purpose of this study was to investigate the contribution of spectral fine structure and spectral envelope cues to recognition of Mandarin lexical tones in normal-hearing and sensorineural hearing-impaired Mandarin-speaking listeners. DESIGN: Four groups of subjects participated in the study, including 20 normal-hearing, 20 moderately, 20 moderately to severely, and 8 severely hearing-impaired listeners. The original speech materials consisted of 16 sets of Mandarin monosyllables spoken by a male and a female. Each monosyllable had four tonal patterns, resulting in a total of 64 combinations of consonants, vowels, and tones. A Linear Predictive Coding (LPC) algorithm was used to create two sets of synthesized materials, including 128 tokens with the original spectral fine structure mixed with the spectral envelope from a different tone, as well as 128 tokens with noise fine structure and the original spectral envelope. All subjects participated in tone recognition tests using the two sets of chimeric tone tokens. Oral responses to tones were recorded and scored as percent correct. RESULTS: Hearing-impaired listeners could take advantage of spectral fine structure in the recognition of lexical tones, but with increasing hearing loss, the ability of hearing-impaired listeners to recognize tones became worse, especially for severely hearing-impaired listeners. Hearing-impaired listeners showed significant differences in tone recognition between the male and female voices. Tone 3 was the easiest tone to perceive, followed by tone 2, whereas tones 1 and 4 were hard for all subjects, particularly when only the spectral envelope cue was available. Hearing-impaired listeners showed a significantly lower level of lexical tone recognition than normal-hearing listeners when using spectral envelope cues in comparison with normal-hearing listeners. CONCLUSIONS: These results demonstrate that the spectral fine structure cue dominates lexical tone recognition for all subjects. Listeners with sensorineural hearing impairment showed reduced ability in the recognition of lexical tones using both spectral fine structure and spectral envelope cues, which may result from their impaired auditory spectral resolution. Copyright © 2010 by Lippincott Williams & Wilkins.


Mfuna-Endam L.,University of Montréal | Zhang Y.,Beijing Institute of Otorhinolaryngology | Zhang Y.,Capital Medical University | Desrosiers M.Y.,University of Montréal
Current Allergy and Asthma Reports | Year: 2011

Suggestion for a potential genetic basis to chronic rhinosinusitis (CRS) is afforded by degree of inheritability suggested from family and twin studies, existence of CRS in simple mendelian diseases, and development of sinusitis as part of the phenotype of certain gene "knockout" murine models. Genetic association studies are expected to identify novel genes associated with CRS and suggest novel mechanisms implicated in disease development. Although these studies are subject to methodologic difficulties, associations of CRS and polymorphisms in more than 30 genes have been published, with single nucleotide polymorphisms in 3 (IL1A, TNFA, AOAH) replicated. While the individual risk conferred by these single nucleotide polymorphisms remains modest, taken as a group, they suggest an important implication of pathways of innate immune recognition and in regulation of downstream signaling in the development of CRS. In a demonstration of these techniques' potential to identify new targets for research, the authors present a functional investigation of LAMB1, the top-rated gene from a pooling-based genome-wide association study of CRS. Upregulation of gene expression in LAMB1 and associated laminin genes in primary epithelial cells from CRS patients implicates the extracellular matrix in development of CRS and offers a new avenue for further study. © 2011 Springer Science+Business Media, LLC.


Zhang Y.,Capital Medical University | Zhang Y.,Beijing Institute of Otorhinolaryngology | Wang C.,Capital Medical University | Zhao Y.,Capital Medical University | And 2 more authors.
American Journal of Rhinology and Allergy | Year: 2013

Background: Forkhead box protein 3 (FOXP3), a transcription factor required for development and function of regulatory T cell, and Epstein-Barr virus-induced gene 3 (EBI3), a downstream target of FOXP3, may be important in the development of chronic rhinosinusitis (CRS) with or without sinonasal polyposis (CRSwNP and CRSsNP, respectively). Our objective was therefore to examine the presence of any associations between specific single nucleotide polymorphisms (SNPs) in/around the FOXP3 and EBI3 genes and risk for CRS in Chinese subjects. A population-based case- control association analysis was performed. Methods: DNA extracted from peripheral blood leukocytes from 667 subjects with CRS (360 CRSwNP and 306 CRSsNP) and 330 healthy controls was assessed for SNPs selected and complemented with tagging SNPs. A total of seven SNPs (4 in FOXP3 and 3 in EBI3 genes) were genotyped and genetic association tests were performed. Results: In the single-locus analyses of CRS risk, the allele frequencies of rs428253 (p = 0.0191) in EBI3 gene and rs2232365 (p = 0.0307) and rs3761548 (p = 0.0221) in FOXP3 gene were significantly different between the CRS cases and the controls. Furthermore, genotype association analysis showed a significant protective effect only between EBI3-rs428253 (CG/CC; p = 0.029) and CRS after adjustment. Similarly, stratified analysis of CRS risk on category showed rs428253 of the EBI3 gene to play a protective role among both CRSwNP (CG/CC; p = 0.002) and CRSsNP (GG/CC; p = 0.023) subjects. Haplotype analysis of the FOXP3 gene region further indicated that CRS risk was higher in individuals carrying the haplotype GG in rs2294018 -rs2232365 block, compared with wild-type AG haplotype, and AG in rs3761548-4824747 block, compared with wild-type CG haplotype. Conclusion: The findings of this study suggest that SNPs in FOXP3 and EBI3 genes modify the risk for development of CRS. Copyright © 2013, OceanSide Publications, Inc., U.S.A.


Hao X.P.,Beijing Institute of Otorhinolaryngology
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery | Year: 2010

To research the effects of chronic suppurative otitis media on bone conduction threshold in old patients. The files of patients with unilateral chronic otitis media were retrospective analyzed, who were all oder than 60 years, who were inpatient in our department since January 2005 to March 2009. Conventional puretone audiometry test was carried out. Bone conduction thresholds were calculated for frequencies of 0.5, 1, 2, and 4 kHz, with comparison between the ear with chronic otitis media and contralateral ear. Thresholds were examined separately for each frequency. The bone conduction threshold for the normal side was lower than those for the ear with chronic otitis media. The threshold shift was statistically significant for each frequency (P < 0.01). There were no differences between the groups when analyzed for the presence of cholesteatoma except at 2 kHz frequencies (Z = -1.975, P = 0.048). There were differences between the groups when analyzed for an interruption of the ossicular chain only at 2 kHz frequencies (Z = -2.721, P = 0.007). There were differences between the groups when the duration of middle ear disease was not same at 1 kHz and 2 kHz frequencies (Z value were -2.877, -2.624, P < 0.01, respectively). This study shows that chronic otitis media can enhance bone conduction threshold for old patients. All measures for early cure should be considered as early as possible in oder patients with chronic otitis media to prevent advance of sensorineural hearing loss.


Xi L.,Beijing Institute of Otorhinolaryngology
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery | Year: 2011

To explore the relationship between allergic rhinitis (AR) and personality traits in nonpsychiatric population of allergic status. Subjects were assigned to the allergic (84 cases) or nonallergic health group (37 cases) on the basis of skin prick test (SPT) and allergic symptoms. The psychological aspects of subjects were assessed by using the minnesota multiphasic personality inventory (MMPI). The allergic group scored higher than the nonallergic group on five clinical scales, hypochondriasis (Hs), depression (D), hysteria (Hy), hypomania (Ma), social introversion (Si), and one research scale manifest anxiety scale (Mas). The differences were significant (t value was respectively 2.169, 2.711, 2.010, 2.577, 2.390 and 2.196, all P < 0.05). In addition, the grade of resultant skin wheal was positively correlated with T scores on the Hs, Hy, psychopathic deviance (Pd) and psychasthenia (Pt). The r value was 0.366, 0.449, 0.345 and 0.355 respectively (all P < 0.05). Subjects with AR show poorer psychological functioning, indicating the close relationship between AR and psychological status.


Zhang Y.,Capital Medical University | Zhang Y.,Beijing Institute of Otorhinolaryngology | Zhang L.,Capital Medical University | Zhang L.,Beijing Institute of Otorhinolaryngology
Allergy, Asthma and Immunology Research | Year: 2014

Allergic rhinitis (AR) is a serious systemic allergic disease, which together with comorbid asthma causes major illness and disability worldwide. Recent epidemiological studies have revealed wide variations in the increasing prevalence of AR and allergies globally, including in China. Despite a markedly higher population than western countries, and a landmass close to Europe in area, little epidemiological data is available on AR in China. Thus, the present study reviewed the prevalence, comorbid allergic diseases, trends and pattern of sensitizing allergens in adults and children suffering from AR in China. Available data indicated that despite variations in the prevalence of AR in different regions of the country, the prevalence of AR has increased in both adults and children over the last 2 decades. Similarly, there has been an increase in a "western"-type lifestyle, industrialization and air pollution over this period, which may have contributed to the increased prevalence of AR observed in China. © The Korean Academy of Asthma, Allergy and Clinical Immunolog.


Lian M.,Capital Medical University | Fang J.,Capital Medical University | Han D.,Capital Medical University | Han D.,Beijing Institute of Otorhinolaryngology | And 4 more authors.
PLoS ONE | Year: 2013

Background: Laryngeal squamous cell carcinoma (LSCC) is the most common type in head and neck squamous cell carcinoma (HNSCC), and the development and progression of LSCC are multistep processes accompanied by changes of molecular biology. Objective: The purpose of this study was to investigate the molecular basis of tumorigenesis and regional lymph node metastasis in LSCC, and provide a set of genes that may be useful for the development of novel diagnostic markers and/or more effective therapeutic strategies. Methods: A total number of 10 patients who underwent surgery for primary laryngeal squamous cell carcinoma were recruited for microarray analysis. LSCC tissues compared with corresponding adjacent non-neoplastic tissues were analysed by Illumina mRNA microarrays, and LSCC tissues with regional lymph node metastasis and LSCC tissues without regional lymph node metastasis were analyzed in the same manner. The most frequently differently expressed genes screened by microarrays were also validated by qRT-PCR in another 42 patients diagnosed for LSCC. Results: Analysed by Illumina mRNA microarrays, there were 361 genes significantly related to tumorigenesis while 246 genes significantly related to regional lymph node metastasis in LSCC. We found that the six genes (CDK1, CDK2, CDK4, MCM2, MCM3, MCM4) were most frequently differently expressed functional genes related to tumorigenesis while eIF3a and RPN2 were most frequently differently expressed functional genes related to regional lymph node metastasis in LSCC. The expressions of these genes were also validated by qRT-PCR. Conclusions: The research revealed a gene expression signature of tumorigenesis and regional lymph node metastasis in laryngeal squamous cell carcinoma. Of the total, the deregulation of several genes (CDK1, CDK2, CDK4, MCM2, MCM3, MCM4, EIF3a and RPN2) were potentially associated with disease development and progression. The result will contribute to the understanding of the molecular basis of LSCC and help to improve diagnosis and treatment. © 2013 Lian et al.

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