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Sun H.,Binzhou Medical University | Qi C.,Chinese Academy of Inspection and Quarantine | Niu Y.,CAS Institute of Mechanics | Kang T.,CAS Institute of Mechanics | And 5 more authors.
PLoS ONE | Year: 2015

Background: Cytomegalovirus (CMV) is the most common infectious cause of mental disability in newborns in developed countries. There is an urgent need to establish an early detection and high-throughput screening method for CMV infection using portable detection devices. Methods: An antibody analysis method is reported for the detection and identification of CMV antibodies in serum using a biosensor based on high spatial resolution imaging ellipsometry (BIE). CMV antigen (CMV-3A) was immobilized on silicon wafers and used to capture CMV antibodies in serum. An antibody against human immunoglobulin G (anti-IgG) was used to confirm the IgG antibody against CMV captured by the CMV-3A. Results: Our results show that this assay is rapid and specific for the identification of IgG antibody against CMV. Further, patient serum was quantitatively assessed using the standard curve method, and the quantitative results were in agreement with the enzyme-linked immunosorbent assay. The CMV antibody detection sensitivity of BIE reached 0.01 IU/mL. Conclusions: This novel biosensor may be a valuable diagnostic tool for analysis of IgG antibody against CMV during CMV infection screening. © 2015 Sun et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Chen X.,CAS Institute of Zoology | Zhang L.,Beijing Inspection and Quarantine Testing Center
Zootaxa | Year: 2016

Three new species of Acidiostigma Hendel from China are described and illustrated. The new species are A. nigrofasciola Chen and Zhang, sp. nov., A. subpostsignatum Chen and Zhao, sp. nov. and A. tongmaiense Chen, sp. nov. An updated key to the known species of the genus is provided. The male of A. bimaculatum W a n g and female of A. postsignatum (Chen) are newly recorded, described and illustrated. The biology and behaviour of A. bimaculatum W a n g and A. postsignatum (Chen) are observed in the field and recorded for the first time. © Copyright 2016 Magnolia Press.

Gu Q.,Zhangjiagang Entry Exit Inspection and Quarantine Bureau | Yi X.,Zhangjiagang Entry Exit Inspection and Quarantine Bureau | Zhang Z.,Beijing Inspection and Quarantine Testing Center | Yan H.,Beijing Inspection and Quarantine Testing Center | And 4 more authors.
Analytical Methods | Year: 2016

In this work a facile, sensitive and reliable method was developed and validated for simultaneous analysis of phytosterols, erythrodiol, uvaol, tocopherols and lutein in olive oils. Analytes extracted from saponified olive oils were directly determined by liquid chromatography-mass spectrometry (LC-MS) with atmospheric pressure chemical ionization (APCI). l-Ascorbic acid sodium salt was added into oils as an antioxidant prior to the saponification process, since tocopherols are sensitive to the effect of light and elevated temperature. We found that using l-ascorbic acid sodium salt made the sample treatment become a facile procedure. In this case we no longer need to use amber glass tubes or enwrap the tubes with aluminum foil. Separation was achieved on a C18 column with a gradient of acetonitrile/water (0.1% formic acid). Erythrodiol and uvaol were quantified separately for the first time in HPLC based analysis. The selected ion monitoring (SIM) mass spectra of 14 analytes were measured by using a mass spectrometer with an APCI ion source. Parameters including the linearity of the standard curves, the detection and quantification limits, recovery, repeatability and reproducibility were evaluated for the validation of the method. © 2016 The Royal Society of Chemistry.

Zhang Z.,Beijing Inspection and Quarantine Testing Center | Yan H.,Beijing Inspection and Quarantine Testing Center | Cui F.,Beijing Inspection and Quarantine Testing Center | Yun H.,Beijing Inspection and Quarantine Testing Center | And 5 more authors.
Food Analytical Methods | Year: 2015

The objective of the present study is to develop and optimize a simple, high-throughput method for determining 14 β-agonists (i.e., banbuterol, brombuterol, cimaterol, cimbuterol, clenbuterol, clenpeterol, clorpenaline, isoxsuprine, mabuterol, mapenterol, ractopamine, salbutamol, terbutaline, and tulobuterol) and two β-blockers (propranolol and penbutolol) in porcine muscle. The samples were pretreated by a modified quick, easy, cheap, efficient, rugged, and safe (QuEChERS) method, and analysis was carried out in a reversed phase HSS T3 C18 column using gradients of acetonitrile and 5 mmol L−1 ammonium acetate (0.1 % formic acid) solution for elution. Ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-LTQ Orbitrap MS, resolution 60,000) was used for qualification and quantification of the 16 target compounds. Under optimized conditions, the limits of detection and quantification obtained ranged from 0.17 to 1.67 μg kg−1 and from 0.56 to 5.00 μg kg−1, respectively. Recoveries for spiking levels of 5.0, 10.0, and 20.0 μg kg−1 ranged from 62.4 to 121.9 %, from 60.4 to 104.3 %, and from 66.5 to 121.3 %, respectively. The relative standard deviations obtained were lower than 20 % for all spiking levels assayed. The proposed method was applied successfully in sample analysis, and satisfactory results were obtained. © 2015 Springer Science+Business Media New York

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