Beijing Forestry University is a multi-disciplinary university under the administration of the Ministry of Education of the People's Republic of China. As one of the key national universities under the Ministry of Education, the university provides advanced education in the fields of forestry, Biological science, Biotechnology, ecology and environmental studies. It was one of the first universities to be admitted into the 211 Project and the 21st-century Educational Vitalization Action Program. Wikipedia.
Song G.,CAS Dalian Institute of Chemical Physics |
Song G.,Beijing Forestry University |
Li X.,CAS Dalian Institute of Chemical Physics
Accounts of Chemical Research | Year: 2015
Conspectus The possibility of developing ne methods for the efficient construction of organic molecules via disconnections other than traditional functional group transformations has driven the interest in direct functionalization of C-H bonds. The ubiquity of C-H bonds makes such transformations attractive, but they also pose several challenges. The first is the reactivity and selectivity of C-H bonds. To achieve this, directing groups (DGs) are often installed that can enhance the effective concentration of the catalyst, leading to thermodynamically stable metallacyclic intermediates. Hoever, the presence of a pendant directing group in the product is often undesirable and unnecessary. This may account for the limitation of applications of C-H functionalization reactions in more common and general uses. Thus, the development of removable or functionalizable directing groups is desirable. Another key problem is that the reactivity of the resulting M-C bond can be lo, hich may limit the scope of the coupling partners and hence limit the reaction patterns of C-H activation reactions.hile the first Cp∗Rh(III)-catalyzed C-H activation of arenes as reported only 7 years ago, significant progress has been made in this area in the past fe years. e began our studies in this area in 2010, and e and others have demonstrated that diversified catalytic functionalization of arenes can be realized using Cp∗Rh(III) complexes ith high reactivity, stability, and functional group compatibility. This Account describes our efforts to solve some of these challenges using Rh(III) catalysis.e fulfilled our design and activation of the arene substrates by taking advantage of the nucleophilicity, electrophilicity, oxidizing potential, and properties of a participating ligand of the directing groups hen the arenes are coupled ith relatively reactive unsaturated partners such as alkenes and alkynes. These in situ funtionalizable roles of the DG alloed extensive chemical manipulation of the initial coupled product, especially in the construction of a diverse array of heterocycles. In the coupling of arenes ith polar coupling partners, the polar Rh(III)-C(aryl) bond shoed higher reactivity as both an organometallic reagent and a nucleophilic aryl source. The polar coupling partners ere accordingly activated by virtue of umpolung, ring strain, and rearomatization. All of these transformations have been made possible by integration of the higher reactivity, stability, and compatibility of Rh(III)-C bonds into catalytic systems. e have demonstrated that to date some of these transformations can be achieved only under rhodium catalysis. In addition, by means of stoichiometric reactions, e have gained mechanistic insights into the interactions beteen the Rh-C bond and the other coupling partners, hich have opened ne avenues in future direct C-H functionalization reactions. © 2015 American Chemical Society.
Wang Q.,Beijing Forestry University |
O'Hare D.,University of Oxford
Chemical Communications | Year: 2013
A facile method for the synthesis of Zn2Al-borate and Mg 3Al-borate layered double hydroxides (LDHs) with extremely high specific surface areas of 458.6 and 263 m2 g-1 and containing delaminated nanosheets is reported. To the best of our knowledge, this is the first report of LDH powders that still remain exfoliated on drying. © 2013 The Royal Society of Chemistry.
He X.Q.,Beijing Forestry University
BMC microbiology | Year: 2014
The aim of this paper was to develop a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid, sensitive and inexpensive detection of astrovirus. The detection limit of LAMP using in vitro RNA transcripts was 3.6 × 10 copies·μL-1, which is as sensitive as the presently used PCR assays. However, the LAMP products could be identified as different colors with the naked eye following staining with hydroxynaphthol blue dye (HNB). No cross-reactivity with other gastroenteric viruses (rotavirus and norovirus) was observed, indicating the relatively high specificity of LAMP. The RT-LAMP method with HNB was used to effectively detect astrovirus in reclaimed water samples. The LAMP technique described in this study is a cheap, sensitive, specific and rapid method for the detection of astrovirus. The RT-LAMP method can be simply applied for the specific detection of astrovirus and has the potential to be utilized in the field as a screening test.
Liu Y.,Beijing Forestry University
PloS one | Year: 2013
Yellow horn (Xanthoceras sorbifolia Bunge) is an oil-rich seed shrub that grows well in cold, barren environments and has great potential for biodiesel production in China. However, the limited genetic data means that little information about the key genes involved in oil biosynthesis is available, which limits further improvement of this species. In this study, we describe sequencing and de novo transcriptome assembly to produce the first comprehensive and integrated genomic resource for yellow horn and identify the pathways and key genes related to oil accumulation. In addition, potential molecular markers were identified and compiled. Total RNA was isolated from 30 plants from two regions, including buds, leaves, flowers and seeds. Equal quantities of RNA from these tissues were pooled to construct a cDNA library for 454 pyrosequencing. A total of 1,147,624 high-quality reads with total and average lengths of 530.6 Mb and 462 bp, respectively, were generated. These reads were assembled into 51,867 unigenes, corresponding to a total of 36.1 Mb with a mean length, N50 and median of 696, 928 and 570 bp, respectively. Of the unigenes, 17,541 (33.82%) were unmatched in any public protein databases. We identified 281 unigenes that may be involved in de novo fatty acid (FA) and triacylglycerol (TAG) biosynthesis and metabolism. Furthermore, 6,707 SSRs, 16,925 SNPs and 6,201 InDels with high-confidence were also identified in this study. This transcriptome represents a new functional genomics resource and a foundation for further studies on the metabolic engineering of yellow horn to increase oil content and modify oil composition. The potential molecular markers identified in this study provide a basis for polymorphism analysis of Xanthoceras, and even Sapindaceae; they will also accelerate the process of breeding new varieties with better agronomic characteristics.
Cui B.-K.,Beijing Forestry University
Mycological Progress | Year: 2013
Antrodia tropica sp. nov. is described and illustrated on the basis of collections originating from Hainan, southern tropical China. Both the morphology and phylogenetic analysis of rDNA ITS sequences support this new species. Morphologically, it is characterized by resupinate basidiocarps, an annual growth habit, greyish to pinkish buff pore surface, a dimitic hyphal system with clamped generative hyphae, and cylindrical to subfusiform basidiospores. The hymenophoral trama is dominated by generative hyphae, whereas skeletal hyphae are dominant in the subiculum. Molecular phylogeny inferred from ITS sequence data suggested a close relationship between A. tropica and two other Antrodia species, including A. huangshanensis reported from China and A. ramentacea found mostly in boreal Eurasia. © 2012 German Mycological Society and Springer.
Cao S.,Beijing Forestry University
Critical Reviews in Environmental Science and Technology | Year: 2011
Since, 1978, China has pursued some of the world's most ambitious conservation programs, including the Three Norths Shelter Forest System Project, Grain for Green Project, and the Natural Forest Conservation Program. After 30 years of experience with these programs, researchers have found that improving agricultural technology and providing appropriate payment for ecosystem service (PES) to farmers displaced by these programs can improve both the environment and society while avoiding the malignant cycle in which poverty leads to environmental destruction, which then deepens poverty. However, flaws in the implementation of these programs mean that it remains necessary to watch for damage to the environment and society caused by inappropriate technology and policy © Taylor & Francis Group, LLC.
Xu Z.,Beijing Forestry University
Molecular genetics and genomics : MGG | Year: 2014
MADS-box genes encode transcription factors that play crucial roles in plant development, especially in flower and fruit development. To gain insight into this gene family in Prunus mume, an important ornamental and fruit plant in East Asia, and to elucidate their roles in flower organ determination and fruit development, we performed a genome-wide identification, characterisation and expression analysis of MADS-box genes in this Rosaceae tree. In this study, 80 MADS-box genes were identified in P. mume and categorised into MIKC, Mα, Mβ, Mγ and Mδ groups based on gene structures and phylogenetic relationships. The MIKC group could be further classified into 12 subfamilies. The FLC subfamily was absent in P. mume and the six tandemly arranged DAM genes might experience a species-specific evolution process in P. mume. The MADS-box gene family might experience an evolution process from MIKC genes to Mδ genes to Mα, Mβ and Mγ genes. The expression analysis suggests that P. mume MADS-box genes have diverse functions in P. mume development and the functions of duplicated genes diverged after the duplication events. In addition to its involvement in the development of female gametophytes, type I genes also play roles in male gametophytes development. In conclusion, this study adds to our understanding of the roles that the MADS-box genes played in flower and fruit development and lays a foundation for selecting candidate genes for functional studies in P. mume and other species. Furthermore, this study also provides a basis to study the evolution of the MADS-box family.
Ma M.-G.,Beijing Forestry University
International Journal of Nanomedicine | Year: 2012
Hierarchically nanosized hydroxyapatite (HA) with flower-like structure assembled from nanosheets consisting of nanorod building blocks was successfully synthesized by using CaCl2, NaH2PO4, and potassium sodium tartrate via a hydrothermal method at 200°C for 24 hours. The effects of heating time and heating temperature on the products were investigated. As a chelating ligand and template molecule, the potassium sodium tartrate plays a key role in the formation of hierarchically nanostructured HA. On the basis of experimental results, a possible mechanism based on soft-template and self-assembly was proposed for the formation and growth of the hierarchically nanostructured HA. Cytotoxicity experiments indicated that the hierarchically nanostructured HA had good biocompatibility. It was shown by in-vitro experiments that mesenchymal stem cells could attach to the hierarchically nanostructured HA after being cultured for 48 hours. Objective: The purpose of this study was to develop facile and effective methods for the synthesis of novel hydroxyapatite (HA) with hierarchical nanostructures assembled from independent and discrete nanobuilding blocks. Methods: A simple hydrothermal approach was applied to synthesize HA by using CaCl2, NaH2PO4, and potassium sodium tartrate at 200°C for 24 hours. The cell cytotoxicity of the hierarchically nanostructured HA was tested by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) assay. Results: HA displayed the flower-like structure assembled from nanosheets consisting of nanorod building blocks. The potassium sodium tartrate was used as a chelating ligand, inducing the formation and self-assembly of HA nanorods. The heating time and heating temperature influenced the aggregation and morphology of HA. The cell viability did not decrease with the increasing concentration of hierarchically nanostructured HA added. Conclusion: A novel, simple and reliable hydrothermal route had been developed for the synthesis of hierarchically nanosized HA with flower-like structure assembled from nanosheets consisting of nanorod building blocks. The HA with the hierarchical nanostructure was formed via a soft-template assisted self-assembly mechanism. The hierarchically nanostructured HA has a good biocompatibility and essentially no in-vitro cytotoxicity. © 2012 Ma, publisher and licensee Dove Medical Press Ltd.
He S.-H.,Beijing Forestry University |
Dai Y.-C.,Beijing Forestry University
Fungal Diversity | Year: 2012
The taxonomy and phylogeny of Hymenochaete and its allied genera in Hymenochaetaceae in China are studied based on morphological characters and molecular analysis. The morphology of more than 1200 specimens were studied and 123 ITS sequences (including 104 new sequences) of 71 taxa and 94 nLSU rDNA sequences (including 51 new sequences) of 78 taxa of Hymenochaetaceae were used in the phylogenetic analysis. Three new species, Hymenochaete huangshanensis, H. minor and H. tropica are introduced. The genus Pseudochaete is supported by the data presented here, with inclusion of an additional seven species transferred herein from Hymenochaete, Hydnochaete and Cyclomyces. Three species of Hydnochaete and two species of Cyclomyces studied nested within the Hymenochaete clade, and combinations of five species from the two former genera to Hymenochaete are proposed. According to our results, Hymenochaete is a morphologically variable genus composed of taxa with corticioid, hydnoid, lamellate and poroid hymenophores, and this would be the same for Pseudochaete with more taxa included. Fifty species of Hymenochaete and eight species of Pseudochaete have so far been found in China, and identification keys to the two genera are provided. © 2012 Mushroom Research Foundation.
Zhang D.,Beijing Forestry University
DNA Research | Year: 2013
Gene-derived simple sequence repeats (genic SSRs), also known as functional markers, are often preferred over random genomic markers because they represent variation in gene coding and/or regulatory regions. We characterized 544 genic SSR loci derived from 138 candidate genes involved in wood formation, distributed throughout the genome of Populus tomentosa, a key ecological and cultivated wood production species. Of these SSRs, three-quarters were located in the promoter or intron regions, and dinucleotide (59.7%) and trinucleotide repeat motifs (26.5%) predominated. By screening 15 wild P. tomentosa ecotypes, we identified 188 polymorphic genic SSRs with 861 alleles, 2-7 alleles for each marker. Transferability analysis of 30 random genic SSRs, testing whether these SSRs work in 26 genotypes of five genus Populus sections (outgroup, Salix matsudana), showed that 72% of the SSRs could be amplified in Turanga and 100% could be amplified in Leuce. Based on genotyping of these 26 genotypes, a neighbour-joining analysis showed the expected six phylogenetic groupings. In silico analysis of SSR variation in 220 sequences that are homologous between P. tomentosa and Populus trichocarpa suggested that genic SSR variations between relatives were predominantly affected by repeat motif variations or flanking sequence mutations. Inheritance tests and single-marker associations demonstrated the power of genic SSRs in family-based linkage mapping and candidate gene-based association studies, as well as marker-assisted selection and comparative genomic studies of P. tomentosa and related species. © 2012 The Author. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.