Beijing Fisheries Research Institute

Beijing, China

Beijing Fisheries Research Institute

Beijing, China
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Luo L.,Beijing Fisheries Research Institute | Ai L.,Chinese Academy of Agricultural Sciences | Liang X.,Chinese Academy of Agricultural Sciences | Hu H.,Beijing Fisheries Research Institute | And 2 more authors.
Aquaculture | Year: 2017

Two diets with the same docosahexaenoic acid:eicosapentaenoic acid ratio (1.8:1), but with different n-3 long-chain polyunsaturated fatty acid (n-3 LC-PUFA) contents [29.5 g/kg (DH) and 17.9 g/kg (DL)], were designed and fed to two groups of 46 female and 30 male Siberian sturgeon (Acipenser baerii) broodstock for 11 months. Sperm and egg quality and reproductive performance indices were recorded during cross-breeding. Larval offspring resulting from cross-matings were divided into the following four groups for a 30-d feeding trial: D1 (DH♀ × DH♂), D2 (DH♀ × DL♂), D3 (DL♀ × DH♂), and D4 (DL♀ × DL♂). The results showed that egg size of the DH group was significantly larger than that of the DL group. With respect to sperm quality, there were no significant differences in wobble, amplitude of lateral head displacement, beat/cross frequency, and mean angular displacement between the two dietary groups; however, a high n-3 LC-PUFA diet increased sperm curvilinear, straight-line, and average path velocities in the DH group. The DH group had significantly lower levels of serum triglyceride, low-density lipoprotein cholesterol, and malonaldehyde, but higher superoxide dismutase level, and total antioxidant capacity compared with the DL group. At 7 and 32 days post hatching, the survival of larvae in groups D1 and D2 was significantly higher than that in groups D3 and D4. The specific growth rate of larvae in group D4 was the lowest among the four groups. The results suggest that n-3 LC-PUFA can improve the reproductive performance of male and female broodstock of Siberian sturgeon and the quality of their offspring, but that n-3 LC-PUFA enrichment of the diet is more critical for female broodstock than for males. © 2017 Elsevier B.V.


Luo L.,Beijing Fisheries Research Institute | Xue M.,Chinese Academy of Agricultural Sciences | Vachot C.,French National Institute for Agricultural Research | Geurden I.,French National Institute for Agricultural Research | Kaushik S.,French National Institute for Agricultural Research
Aquaculture | Year: 2014

This study examined the effect of dietary medium-chain triglycerides supplied by coconut oil on postprandial plasma metabolite profiles in rainbow trout. The fish (initial body weight 71.3 ± 0.3. g, 17. °C) were fed one of four practical diets containing either 5% fish oil (FO low-fat, FL), 15% fish oil (FO high-fat, FH), 5% coconut oil (CO low-fat, CL) or 15% coconut oil (CO high-fat, CH) for 3. weeks. At the end of the trial, the fish were weighed and plasma sampled to determine glucose, non-esterified fatty acids (NEFA), triglyceride (TG), cholesterol, high density lipoprotein-cholesterol (HDL-cholesterol), and myeloperoxidase (MPO) at 3, 6, 9, 12, 15 and 24. h after the last meal. Plasma total ketone bodies (KB) were determined at 6, 12 and 24. h after meal. Blood nitroblue tetrazolium (NBT) tests were also performed in samples withdrawn at 24. h after meal.Plasma glucose was higher in fish fed the low fat level diet than those fed high fat level, and peaked at postprandial 9-12. h. Fish fed CH showed higher plasma TG than CL at 3. h after meal, and there was no significant difference in plasma TG at the other time points. The peak of TG appeared 12. h after the meal. No clear pattern was found for cholesterol and HDL-cholesterol (HDL-C) in any of the groups. However, fish fed diet FH had the highest postprandial plasma HDL-cholesterol level and HDL-C/cholesterol ratio. The peak of NEFA was observed at 12-15. h after meal and plasma NEFA of fish fed CH was the highest. Plasma total KB decreased with postprandial time, and fish of FH groups had higher KB than that of CL group at 6. h. Besides, NBT in fish fed FH was significantly higher than that of CH, but there were no differences in MPO between groups. In summary, time-course changes in plasma profiles related to dietary fat level were as expected whereas those related to dietary fat source were relatively small. © 2013 Elsevier B.V.


Zhang Z.,Peking University | Jia C.,Beijing Fisheries Research Institute | Hu Y.,Peking University | Sun L.,Peking University | And 8 more authors.
Toxicology Letters | Year: 2012

Salicylate esters (SEs), a class of chemicals extensively used as flavor and fragrance additives in foods, beverages and a wide variety of consumer products, are suspected to have estrogenic activity based on chemical analysis of in silica molecular docking. We evaluated the estrogenic potentials of phenyl salicylate (PhS), benzyl salicylate (BzS), phenethyl salicylate (PES), ethyl salicylate (ES) and methyl salicylate (MS) using an in vitro human estrogen receptor α (hERα)-coactivator recruiting assay and in vivo immature rodent uterotrophic bioassays. We found that PhS, BzS and PES showed obvious in vitro hERα agonistic activities; BzS in particular exhibited a higher estrogenic activity compared to bisphenol A (BPA). The uterine weights were significantly increased in mice treated with 11.1, 33.3, 100 and 300mg/kg/day BzS and 33.3mg/kg/day PES and rats treated with 3.7, 11.1, 33.3 and 100mg/kg/day BzS for 3 days (P<0.05). Finally, we transformed the daily intakes and the dermal exposures of SEs in the real world into estradiol equivalent concentrations (EEQs). We found that the EEQ of BzS daily intake in consumers in the U.S. and the EEQs of dermal BzS and PES exposure among high-volume users worldwide were higher than the maximum secure daily estradiol intake recommended by the U.S. Food and Drug Administration (FDA). In particular, the EEQ for dermal BzS exposure was up to 162ng EEQ/kg, which is 3.3 times higher than the maximal acceptable daily E 2 intake recommended by the Joint FAO/WHO Expert Committee on Food Additives (JECFA). © 2011 Elsevier Ireland Ltd.


Luo L.,Beijing Fisheries Research Institute | Wang J.,Chinese Academy of Agricultural Sciences | Pan Q.,University of South China | Xue M.,Chinese Academy of Agricultural Sciences | And 3 more authors.
Aquaculture Research | Year: 2012

Nutrient apparent digestibility coefficients (ADCs) of pet food grade poultry by-product meal (PBM) were determined for black tiger prawn, Penaeus monodon and Pacific white shrimp, Litopenaeus vannamei by the indirect method (reference diet and test diet at 7:3 ratio). Subsequently, an 8-week growth trial was conducted to evaluate the effects of substitution of fishmeal (FM) with PBM in diets of P. monodon (initial weight = 0.21 ± 0.01 g). In the growth trial, six isonitrogenous and isoenergetic diets PBM0, PBM25, PBM50, PBM75, PBM100 and PBMA100, containing a gradient of PBM 0, 88.7, 177.4, 266, 354.7 and 354 g kg-1 to replace 0, 92.5, 185, 277.5, 370 and 370 g kg-1 FM were fed to four replicate groups respectively. The diet PBMA100 was supplemented with DL-Met to be similar to PBM0. The results showed that both P. monodon and L. vannamei had relatively high ADC of crude protein (77.6% and 84.2% respectively) and gross energy (72.8% and 84.0% respectively) for PBM. Litopenaeus vannamei showed significantly higher digestion ability for PBM than P. monodon (P < 0.05). In growth trial, no significant difference in growth performance was observed among shrimp fed the experimental diets. DL-Met supplementation did not improve the growth of P. monodon. PBM is a suitable protein ingredient for P. monodon feeds and can be used up to 354.7 g kg-1 to totally replaced FM. © 2011 Blackwell Publishing Ltd.


Liu X.,China Agricultural University | Jiang Y.,China Agricultural University | Shen S.,China Agricultural University | Luo Y.,China Agricultural University | Gao L.,Beijing Fisheries Research Institute
LWT - Food Science and Technology | Year: 2015

Quality changes in total aerobic counts (TAC), electrical conductivity (EC), K-value and sensory assessment (SA) of rainbow trout (Oncorhynchus mykiss) fillets during storage at 282, 279, 276, 273 and 270K were determined. Simultaneously, Arrhenius model and feed-forward artificial neuronal network (ANN) were established to predict changes of rainbow trout fillets during storage, and a comparative study between these two models was also performed. The relative error between predicted and experimental value was used as the comparative parameter. The results showed that TAC, EC and K-value increased with storage time, while SA decreased with time. The change rate of all indicators increased as a function of temperature. Arrhenius models based on EC and TAC were acceptable, while those based on SA and K-value showed poor performances in some days. By contrast, ANN was more effective to predict changes in TAC, EC, K-value and SA throughout the storage, with relative errors all below 10%. Therefore, ANN could be a potential tool in modeling quality changes of rainbow trout fillets within 270-282K. © 2014 Elsevier Ltd.


PubMed | Beijing Fisheries Research Institute and China Agricultural University
Type: Journal Article | Journal: Journal of food science and technology | Year: 2015

In order to evaluate the effect of super chilling (-3C) and chilled (3C) storage on the quality of rainbow trout fillets, total volatile base nitrogen (TVB-N), drip loss, pH, electric conductivity (EC), total aerobic count (TAC), K and related values, adenosine triphosphate (ATP) and related compounds, color and sensory score were determined and correlation between these indicators were analyzed. According to the comprehensive evaluation of TAC, K value and sensory score, the limit for acceptability of rainbow trout fillets was 5days at 3C and 11days at -3C. Additionally, the correlation coefficients between TVB-N and other freshness indicators (TAC, K value, sensory score) were relatively low. TVB-N may be inadequate for evaluating freshness changes of rainbow trout fillets compared with other indicators. Among the K and related values, H value was a better freshness indicator in rainbow trout fillets during chilled and super chilling storage for its better correlation coefficients with other freshness indicators. Super chilling storage could extend the shelf life of rainbow trout fillets by 6days compared to chilled storage.


PubMed | Beijing Fisheries Research Institute and China Agricultural University
Type: | Journal: Carbohydrate polymers | Year: 2015

Chondroitin sulfates (CSs) were extracted from sturgeon skull and backbone, and their chemical composition, anticoagulant, anti-platelet and thrombolysis activities were evaluated. The average molecular weights of CS from sturgeon skull and backbone were 38.5kDa and 49.2kDa, respectively. Disaccharide analysis indicated that the sturgeon backbone CS was primarily composed of disaccharide monosulfated in position four of the GalNAc (37.8%) and disaccharide monosulfated in position six of the GalNAc (59.6%) while sturgeon skull CS was primarily composed of nonsulfated disaccharide (74.2%). Sturgeon backbone CS showed stronger antithrombotic effect than sturgeon skull CS. Sturgeon backbone CS could significantly prolong activated partial thromboplastin time (APTT) and thrombin time (TT), inhibited ADP-induced platelet aggregation and dissolved platelet plasma clots in vitro. The results suggested that sturgeon backbone CS can be explored as a functional food with antithrombotic function.


Trudeau V.L.,University of Ottawa | Martyniuk C.J.,University of New Brunswick | Zhao E.,University of Ottawa | Hu H.,Beijing Fisheries Research Institute | And 3 more authors.
General and Comparative Endocrinology | Year: 2012

Numerous small potentially bioactive peptides are derived from the selective processing of the ~600 amino acid secretogranin II (SgII) precursor, but only the 31-42 amino acid segment termed secretoneurin (SN) is well-conserved from sharks to mammals. Both SNa and SNb paralogs have been identified in some teleosts, likely arising as a result of the specific genome duplication event in this lineage. Only one copy of the putative lamprey SgII (188 amino acids) could be identified which gives rise to a divergent agnathan SN that contains the signature YTPQ-X-LA-X 7-EL sequence typical of the central core of all known SN peptides. In rodent models, SN has regulatory effects on neuroinflammation and neurotransmitter release, and possesses therapeutic potential for the induction of angiogenesis. The wide distribution of SN in neuroendocrine neurons and pituitary cells suggests important endocrine roles. The clearest example of the endocrine action of SN is the stimulatory effects on pituitary luteinizing hormone release from goldfish pituitary and mouse LβT2 gonadotroph cells, indicative of an important role in reproduction. Several lines of evidence suggest that the SN receptor is most likely a G-protein coupled protein. Microarray analysis of SN effects on dispersed goldfish pituitary cells in vitro reveals novel SN actions that include effects on genes involved in notch signaling and the guanylate cyclase pathway. Intracerebroventricular injection of SN increases feeding and locomotory behaviors in goldfish. Given that SgII appeared early in vertebrate evolution, SN is an old peptide with emerging implications as a new multifunctional hormone. © 2011 Elsevier Inc.


Lin X.,Southwest University | Chen W.,Southwest University | Lin S.,Southwest University | Luo L.,Beijing Fisheries Research Institute
Aquaculture Research | Year: 2015

An 8-week feeding trial was conducted to evaluate the effect of dietary cecropin on growth performance, non-specific immunity and disease resistance of hybrid tilapia (Oreochromis niloticus × O. aureus). A basal diet was supplemented with 0 (control), 75, 150 and 225 mg kg-1 cecropin to formulate four experimental diets. The results showed that final body weight and specific growth rate obtained with diet 150 mg kg-1 cecropin were significantly higher (P < 0.05) than that obtained with diets 0 and 75 mg kg-1, whereas there was no difference between 150 and 225 mg kg-1. Feed conversion efficiency was improved by increasing the dietary cecropin content (P < 0.05). However, feeding intake was reduced as dietary cecropin increased. Serum lysozyme, alkaline phosphatase, catalase and superoxide dismutase activity were significantly increased with the increased levels of dietary cecropin (P < 0.05), and reached a maximum at level of 150 mg kg-1 cecropin, then those values no longer increased. Moreover, the dietary cecropin supplementation level also exhibited a decrease in the cumulative mortality rates compared with the controls when challenged with Aeromonas veronii. The results suggested that the dietary supplementation of cecropin could improve the growth, non-specific immunity indicators and resistance of hybrid tilapia against A. veronii. © 2015 John Wiley & Sons Ltd.


Yang G.,Beijing Fisheries Research Institute | Xu S.,Beijing Fisheries Research Institute
ITME 2011 - Proceedings: 2011 IEEE International Symposium on IT in Medicine and Education | Year: 2011

Specific dynamic action (SDA), the metabolic phenomenon resulting from the digestion and assimilation of a meal, is generally influenced by temperature. The effects of temperature (5.50.5, 8.50.5, 11.50.5, 14.50.5, 17.50.5C) on SDA of brook trout (Salvelinus fontinalus Mitchill) was evaluated, by measuring the temporal pattern of the oxygen consumption rates, after feeding. Peak VO 2 (highest recorded oxygen consumption rate following feeding) and SDAE (energy expended on SDA) increased slowly to a maximum at 14.50.5C, and then decreased at 17.50.5 C. The relationships between Peak VO 2 (mgkg 1h 1), the SDA E (kJkg 1) and temperature (t, C) were respectively described as: Peak VO 2 = 0.1332t 4 + 5.6612t 3 86.317t 2 + 581.33t 1275.3, SDA E = 23.59t 4 + 999.28t 3 15303t 2 + 101913t 229665 (n=650, R 2 = 1, 4.5#60;t#60;17.5). The results of Peak VO 2 and SDA E showed that the optimum growth of brook trout occurred at 14.50.5. Duration decreased from 280.7h to 160.6h as the temperature increased from 5.50.5 to 17.50.5C. The relationship between duration (h) and temperature (t, C) was described as: duration = 0.0051t 4 + 0.2305t 3 3.767t 2 + 25.724t 33.167, (n=650, R 2 = 1, 4.5

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