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Fang Y.,CAS Changchun Northeast Institute of Geography and Agroecology | Zhong R.,CAS Changchun Northeast Institute of Geography and Agroecology | Chen L.,Beijing Embryo Biotech Center | Feng C.,Beijing Embryo Biotech Center | And 2 more authors.
Small Ruminant Research | Year: 2015

The aim of this study was to evaluate the effects of astaxanthin (AST) supplementation of semen extender on the quality of ram semen during storage at 4. °C for periods up to 72. h. Semen samples were obtained from five Small-tail Han rams by use of artificial vagina during normal reproduction season (August to October in 2014), and diluted with glucose-egg yolk buffer supplemented with 0 (control), 0.5, 1, 2, or 4. μM AST. The results showed that supplementation of the extender with 2. μM and 4. μM of AST increased sperm vitality and plasma membrane integrity (P< 0.05), and significantly reduced malondialdehyde and reactive oxygen species levels during the 72. h storage period (P< 0.05). Acrosome integrity did not differ among treatments. After 72. h of storage in extender, samples were analyzed with computer-assisted semen analysis. The motility of sperm in the 2. μM and 4. μM AST treatment groups increased significantly (P< 0.05) compared to the control treatment, while curvilinear velocity, progressive velocity, and path velocity were not affected. In conclusion, the present study demonstrated that supplementing semen extender with AST can improve ram semen preservation via protecting plasma membrane integrity. © 2015 .

Deng Q.,Jilin University | Gao Y.,Jilin University | Jiang H.,Jilin University | Chen C.Z.,Jilin University | And 4 more authors.
Genetics and Molecular Research | Year: 2015

This study was designed to examine a single nucleotide polymorphism (SNP) in the HIF-3α gene in three hundred Changbaishan black cattle using PCR-restriction fragment length polymorphism to determine whether there is an association between this SNP and superovulation. The cloning and sequencing results indicate that the polymorphism is due to a point mutation at the 278-bp position in the HIF-3α gene, resulting in 3 genotypes (AA, AB, and BB). Association analysis indicated that the polymorphism has a significant effect on the number of unfertilized embryos (NUE) (P < 0.05) in the cattle. Cattle with genotype BB had a higher NUE than those with genotype AA, but the difference in NUE between AB and AA or BB was not significant. The polymorphism also has a highly significant effect on the number of degenerative embryos (NDE) and the number of total embryos (NTE) (P < 0.01). Genotype BB was associated with a higher NDE than AA, but the difference in NDE between AB and AA or BB was not significant. Genotype BB showed a higher NTE than AA or AB, but the difference in NTE between AA and AB was not significant. No significant conclusions could be drawn with respect to susceptibility to other traits. HIF-3α could serve as a useful biomarker for donor selection, superovulation improvement, and assisted fertility. © FUNPEC-RP.

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