PubMed | University of New South Wales, Binhai Genomics Institute, Beijing Electrical Power Hospital and Peking Union Medical College
Type: Journal Article | Journal: PloS one | Year: 2016
The -hemolysin, encoded by the hla gene, is a major virulence factor in S. aureus infections. Changes in key amino acid residues of -hemolysin can result in reduction, or even loss, of toxicity. The aim of this study was to investigate the diversity of the hla gene sequence and the relationship of hla variants to the clonal background of S. aureus isolates. A total of 47 clinical isolates from China were used in this study, supplemented with in silico analysis of 318 well-characterized whole genome sequences from globally distributed isolates. A total of 28 hla genotypes were found, including three unique to isolates from China, 20 found only in the global genomes and five found in both. The hla genotype generally correlated with the clonal background, particularly the multilocus sequence type, but was not related to geographic origin, host source or methicillin-resistance phenotype. In addition, the hla gene showed greater diversity than the seven loci utilized in the MLST scheme for S. aureus. Our investigation has provided genetic data which may be useful for future studies of toxicity, immunogenicity and vaccine development.
Yu L.-Y.,Beijing Electrical Power Hospital |
Pei Y.,Chinese PLA General Hospital
Chinese Medical Journal | Year: 2015
Objective: To analyze the mechanism of neuroprotection of insulin and which blood glucose range was benefit for insulin exerting neuroprotective action. Data Sources: The study is based on the data from PubMed. Study Selection: Articles were selected with the search terms “insulin”, “blood glucose”, “neuroprotection”, “brain”, “glycogen”, “cerebral ischemia”, “neuronal necrosis”, “glutamate”, “γ-aminobutyric acid”. Results: Insulin has neuroprotection. The mechanisms include the regulation of neurotransmitter, promoting glycogen synthesis, and inhibition of neuronal necrosis and apoptosis. Insulin could play its role in neuroprotection by avoiding hypoglycemia and hyperglycemia. Conclusions: Intermittent and long-term infusion insulin may be a benefit for patients with ischemic brain damage at blood glucose 6–9 mmol/L. © 2015, Chinese Medical Association. All rights reserved.
Li Y.,Chinese PLA Medical School |
Wang Y.,Beijing Electrical Power Hospital |
Ma L.,Chinese PLA Medical School
Genomics, Proteomics and Bioinformatics | Year: 2014
Although molecular mechanisms of heat stroke under physiological and pathological conditions have not yet been elucidated, a novel disease-associated gene encoding a calcium-binding protein, calsequestrin-1 (CASQ1), was suggested relevant based on results from a transgenic murine model. Here, we show the association between single nucleotide polymorphisms (SNPs) of CASQ1 and physiological parameters for heat stroke from a study involving 150 patients. Pooled DNA from heat stroke patients were subjected to sequencing and 3 SNPs were identified. Genotypes were assigned for all patients according to g. 175A>G, one SNP which leads to a nonsynonymous substitution (N59D) in the first exon of human CASQ1 gene. We analyzed the genotypic data with a linear model based on significance scores between SNP (175A>G) and heat stroke parameters. As a result, we found a significant association between SNP A175G and heat stroke (P<. 0.05). Further bioinformatics analysis of the 1-Mb flanking sequence revealed the presence of two genes that encode DDB1 and CUL4 associated factor 8 (DCAF8), and peroxisomal biogenesis factor 19 (PEX19), respectively, which might be functionally related to CASQ1. Our results showed that the blood calcium of patients with allele D increased significantly, compared to patients with allele N (P<. 0.05), which may result from the decreased calcium in muscle, suggesting that N59D in CASQ1 might account for the dysfunction of CASQ1 in calcium regulation during heat stroke. © 2014.
Guo L.,Shanxi Normal University |
Zhang R.,Shanxi Normal University |
Guo L.L.,Beijing Electrical Power Hospital |
Niu S.,Shanxi Normal University
Computational and Theoretical Chemistry | Year: 2014
Using the CO oxidation as a chemical probe, we perform a comprehensive ab initio study of catalytic activities of subnanometer AlPtn (n=1-3) clusters. A Langmuir-Hinshelwood (LH) mechanism is proposed, which offers insights into the fundamental mechanism for CO oxidation on nanosized AlPtn clusters. It is shown that mixing two different metals (Al and Pt) can have beneficial effects on the catalytic activity. The alloyed AlPt2 cluster is proposed as the best effective nanocatalyst. © 2014 Elsevier B.V.
PubMed | Nantong University, Beijing Electrical Power Hospital and Soochow University of China
Type: | Journal: Neuroscience bulletin | Year: 2016
Itch (pruritus) is one of the most disabling syndromes in patients suffering from skin, liver, or kidney diseases. Our previous study highlighted a key role of oxidative stress in acute itch. Here, we evaluated the effects of antioxidants in mouse models of acute and chronic itch and explored the potential mechanisms. The effects of systemic administration of the antioxidants N-acetyl-L-cysteine (NAC) and N-tert-butyl--phenylnitrone (PBN) were determined by behavioral tests in mouse models of acute itch induced by compound 48/80 or chloroquine, and chronic itch by treatment with a mixture of acetone-diethyl-ether-water. We found that systemic administration of NAC or PBN significantly alleviated compound 48/80- and chloroquine-induced acute itch in a dose-dependent manner, attenuated dry skin-induced chronic itch, and suppressed oxidative stress in the affected skin. Antioxidants significantly decreased the accumulation of intracellular reactive oxygen species directly induced by compound 48/80 and chloroquine in the cultured dorsal root ganglia-derived cell line ND7-23. Finally, the antioxidants remarkably inhibited the compound 48/80-induced phosphorylation of extracellular signal-regulated kinase in the spinal cord. These results indicated that oxidative stress plays a critical role in acute and chronic itch in the periphery and spinal cord and antioxidant treatment may be a promising strategy for anti-itch therapy.
Lu N.-F.,Beijing Electrical Power Hospital |
Zheng R.-Q.,Yangzhou University |
Lin H.,Yangzhou University |
Shao J.,Yangzhou University |
And 2 more authors.
American Journal of Emergency Medicine | Year: 2015
Background Sepsis bundles can decrease mortality in patients with severe sepsis or septic shock. However, current methods of measuring pressure, such as central venous pressure, are inadequate. This study investigated the effect of improved sepsis bundles informed by pulse-indicated continuous cardiac output. Methods We compared the outcome of treatment with sepsis bundles informed by either conventional pressure measurements or pulse-indicated continuous cardiac output. Patients in 2 groups received fluid resuscitation, standard antibiotics, and oxygen therapy. Results A total of 105 patients with septic shock were randomly divided into 2 groups: the conventional sepsis bundle group (n = 52) or the improved sepsis bundle group (ISBG, n = 53). The ISBG significantly reduced the mean Acute Physiology and Chronic Health Evaluation II and Sepsis-related Organ Failure Assessment scores. Significantly fewer ISBG-treated patients received vasoactive drugs compared to conventional sepsis bundle group-treated patients. In addition, patients in the ISBG exhibited a significantly increased arterial blood lactate clearance rate and required less total fluid resuscitation and a shorter duration of mechanical ventilation and stay in the intensive care unit. Conclusions Pulse-indicated continuous cardiac output-directed sepsis bundles can reduce the severity of septic shock, provide more accurate fluid resuscitation, and reduce the duration of mechanical ventilation and stay in the intensive care unit. © 2015 Elsevier Inc.
Xu F.,Beijing Electrical Power Hospital |
Zhao M.-Y.,Beijing Electrical Power Hospital |
Liu J.,Beijing Electrical Power Hospital
International Eye Science | Year: 2016
AIM: To study the changes of choroidal thickness and hemodynamic parameters in patients with diabetic retinopathy and their influencing factors. METHODS: From January 2013 to January 2015, 100 patients (100 eyes) with type 2 diabetes were divided into 3 groups: 34 patients without diabetic retinopathy (NDR), 36 patients with non proliferative diabetic retinopathy (NPDR) group, and 30 patients with proliferative diabetic retinopathy (PDR). According to the results of OCT, the patients with diabetic retinopathy were divided into 2 groups: diabetic macular edema (DME) group (28 cases), and 38 cases without diabetic macular edema. During the same period in our hospital 35 subjects for physical examination were selected as the control group. The hemodynamic parameters of the posterior ciliary artery in different groups of patients at different distance from the center of the macular were compared, analyzing influencing factors. RESULTS: With the aggravation of diabetic retinopathy, the choroidal thickness in different distance from the center of the macular decreased. The choroidal thickness of NPDR and PDR group were thinner than that of the control group (P<0.05). The choroidal thickness of NDR group was not different from the control group (P>0.05). There was no significant difference in the choroidal thickness between the DME patients and non DME patients (P>0.05). Pearson correlation analysis showed that there was no significant correlation between choroidal thickness of patients with diabetic retinopathy and diabetic duration, fasting blood glucose, HbA1c, eye axis length, systolic blood pressure and diastolic blood pressure (P>0.05), but there was a correlation with BCVA (logMAR) (P<0.01). EDV and PSV in NDR group and NPDR group were significantly lower than those in control group, RI was higher than that in control group. PSV and EDV in PDR group were significantly lower than those in other three groups, RI was higher than the other three groups, and the difference was statistically significant (P<0.05). CONCLUSION: With the severity of retinopathy in type 2 diabetic patients, the choroidal thickness decreased, and the thickness of the choroid is beneficial in the comprehensive analysis of 2 diabetic retinopathy. Copyright 2016 by the IJO Press.
Yu L.,Beijing Electrical Power Hospital |
Jiao X.,Beijing Tiantan Hospital |
He Y.,Beijing Tiantan Hospital
International Journal of Clinical and Experimental Medicine | Year: 2016
Moyamoya patients underwent cerebral revascularization surgery had higher risk for postoperative complications. Cerebral autoregulation impaired may be the main mechanism for postoperative complications. In our study, we used correlation coefficient r between cerebral tissue oxygenation index (TOI) and mean arterial blood pressure (MAP) to assessing of cerebral autoregulation. Total 30 moyamoya patients measured MAP and TOI. Spearman rank correlation coefficient r was calculated between MAP and TOI. 8 (26.67%) patients had postoperative neurological deterioration. The absolute value of r above 0.414 was adopted as diagnostic standard to predict postoperative neurological deterioration. Its sensitivity was 0.875, and specificity was 0.864. Correlation coefficients r was best predictors of postoperative neurological deterioration in our study. The meaning of correlation coefficient r need further study in a larger sample. © 2016, E-Century Publishing Corporation. All rights reserved.
Dong J.-D.,Beijing Electrical Power Hospital |
Huang J.-H.,Beijing Electrical Power Hospital |
Gao F.,Beijing Electrical Power Hospital |
Zhu Z.-H.,Beijing Electrical Power Hospital |
Zhang J.,Capital Medical University
Vascular | Year: 2011
The aim of the study was to construct small-diameter vascular grafts using canine mesenchymal stem cells (cMSCs) and a pulsatile flow bioreactor. cMSCs were isolated from canine bone marrow and expanded ex vivo. cMSCs were then seeded onto the luminal surface of decellularized arterial matrices, which were further cultured in a pulsatile flow bioreactor for four days. Immunohistochemical staining and scanning electron microscopy was performed to characterize the tissue-engineered blood vessels. cMSCs were successfully seeded onto the luminal surface of porcine decellularized matrices. After four-day culture in the pulsatile flow bioreactor, the cells were highly elongated and oriented to the flow direction. Immunohistochemistry demonstrated that the cells cultured under pulsatile flow expressed Von Willebrand factor, an endothelial cell marker. In conclusion, cMSCs seeded onto decellularized arterial matrices could differentiate into endothelial lineage after culturing in a pulsatile flow bioreactor, which provides a novel approach for tissue engineering of small-diameter blood vessels. © 2011 Royal Society of Medicine Press. All rights reserved.
Lai G.-H.,Capital Medical University |
Tang Y.-Z.,Capital Medical University |
Wang X.-P.,Capital Medical University |
Qin H.-J.,Beijing Electrical Power Hospital |
Ni J.-X.,Capital Medical University
Medicine (United States) | Year: 2015
This article evaluates the long-term outcomes of computed tomography (CT)-guided percutaneous radiofrequency thermocoagulation (PRT) for patients with recurrent trigeminal neuralgia (TN) after microvascular decompression (MVD). This is a retrospective study of 41 patients with intractable TN who after MVD underwent CT-guided PRT procedures between 2002 and 2012. The mean length of follow-up after PRT was 44.4 months. Immediate pain relief was in 37 patients (90.2%); the percentage of patients who remained in "excellent" or "good" pain relief condition after CT-guided PRT procedure was 85% at 1 year, 80% at 2 years, 51% at 5 years, and 41% at 10 years. Six patients received the second PRT and all achieved "excellent" or "good" pain relief. In total, 34 of these patients (82.9%) received multi-PRT procedure and remained satisfied with their pain relief during the follow-up period. Postoperative complications included facial numbness in 36 patients, limited eyes opening in 1 patient, ear paresthesia in 1 patient, no tears in 1 patient, and taste hypesthesia in 1 patient; these symptoms were all improved in the process of follow-up and their life had not severely affected. No mortality was observed during and after CT-guided PRT procedures. CT-guided PRT should be considered as an alternative treatment for patients with recurrent TN after MVD. Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved.