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Liu W.,Peking University | Hu D.,Peking University | Zhu T.,Peking University | Ma Z.,Peking University | And 8 more authors.
American Journal of Cardiology | Year: 2013

Hypertrophic cardiomyopathy (HC) is a hereditary heterogeneous cardiovascular disorder. Existing data have been of predominantly Caucasian samples, and a large study is needed in Chinese population. The present study was intended to explore the genetic basis and clinical characteristics correlated with different genotypes in a large cohort of Chinese patients. Direct gene sequencing of β-myosin heavy chain (MYH7), myosin binding protein-C (MYBPC3), and cardiac troponin T (TNNT2) was performed in 136 unrelated Chinese HC patients. Clinical evaluations were conducted. In total, 32 mutations were identified in 36 patients (27%), including 10 novel ones. Distribution of mutations was 56% (MYBPC3), 31% (MYH7), and 13% (TNNT2), respectively. Double mutations were identified in 3% patients. The occurrence of HC-associated sarcomeric mutations was associated with an earlier age of onset, increased left ventricular hypertrophy, a higher incidence of syncope, previous family history, and sudden cardiac death. No statistical difference was identified in patients carrying MYBPC3 and MYH7 mutations with regard to clinical characteristics and outcomes. Patients with double mutations were associated with malignant progression in the study. In conclusion, MYBPC3 is the most predominant gene in HC. Multiple mutations are common in MYH7, MYBPC3, and TNNT2. The present study suggests a large diversity of HC and a prognostic role of genotype. © 2013 Elsevier Inc. All rights reserved.


Dong X.,Shanxi Normal University | Guo L.,Shanxi Normal University | Wen C.,Shanxi Normal University | Ren N.,Shanxi Normal University | And 3 more authors.
Research on Chemical Intermediates | Year: 2015

The CO preferential oxidation reaction (PROX) is particularly well suited for hydrogen purification for proton exchange membrane fuel cell applications. In this work, the mechanism of CO-PROX catalyzed by CunPt (n = 3-12) clusters has been studied by density functional theory calculations. The calculated results indicate that the most favored adsorption site ofH2 for all clusters is on the Pt sites, and O2 prefers to bind on Cu sites and CO bind on Pt sites. The lowest energy barrier for hydrogen dissociation is 0.02 eV. Smaller H-Pt bond length of CunPtH2 corresponds to larger H-H bond length. CO-PROX occurs via the main intermediates of COOH and OH. Cu6Pt is proposed as the most effective catalyst for CO-PROX. To understand the high catalytic activity of CunPt clusters, the nature of the interaction between adsorbate and substrate is also analyzed by detailed electronic local density of states. These findings enrich applications of Cu-based materials to the field of high-activity catalysts. © Springer Science+Business Media Dordrecht 2015.


Li Y.,Chinese PLA Medical School | Wang Y.,Beijing Electrical Power Hospital | Ma L.,Chinese PLA Medical School
Genomics, Proteomics and Bioinformatics | Year: 2014

Although molecular mechanisms of heat stroke under physiological and pathological conditions have not yet been elucidated, a novel disease-associated gene encoding a calcium-binding protein, calsequestrin-1 (CASQ1), was suggested relevant based on results from a transgenic murine model. Here, we show the association between single nucleotide polymorphisms (SNPs) of CASQ1 and physiological parameters for heat stroke from a study involving 150 patients. Pooled DNA from heat stroke patients were subjected to sequencing and 3 SNPs were identified. Genotypes were assigned for all patients according to g. 175A>G, one SNP which leads to a nonsynonymous substitution (N59D) in the first exon of human CASQ1 gene. We analyzed the genotypic data with a linear model based on significance scores between SNP (175A>G) and heat stroke parameters. As a result, we found a significant association between SNP A175G and heat stroke (P<. 0.05). Further bioinformatics analysis of the 1-Mb flanking sequence revealed the presence of two genes that encode DDB1 and CUL4 associated factor 8 (DCAF8), and peroxisomal biogenesis factor 19 (PEX19), respectively, which might be functionally related to CASQ1. Our results showed that the blood calcium of patients with allele D increased significantly, compared to patients with allele N (P<. 0.05), which may result from the decreased calcium in muscle, suggesting that N59D in CASQ1 might account for the dysfunction of CASQ1 in calcium regulation during heat stroke. © 2014.


Wang L.,Beijing University of Chinese Medicine | Han L.,Beijing University of Chinese Medicine | Liu P.,Beijing Electrical Power Hospital | Yang J.,Beijing University of Chinese Medicine | And 7 more authors.
European Journal of Integrative Medicine | Year: 2016

Introduction: Moxibustion is commonly used as a traditional Chinese medicine treatment. The aim was to investigate how the exposure of moxa smoke influences sperm parameters, testis index and serum sex hormones in Wistar male rats. Methods: 48 Wistar rats were randomly assigned, n = 12/group, to a control group or one of three moxa-smoke groups: M1 (low concentration, 0.4% of optical density), M2 (middle concentration, 2%) and M3 (high concentration, 15%). Rats in moxa smoke groups were exposed at the respective concentrations of M1 (0.4% of optical density), M2 (2%) and M3 (15%) for 12 weeks, at 5 days per week and 200 min per day. Dynamic analyses of sperm fluid were used to determine sperm concentration, progressive motility, sperm motility, linearity (LIN), wobble (WOB), straightness (STR), velocity of curved line (VCL), velocity of straight line (VSL) and velocity of average path (VAP). The testis index (TI) was measured, and serum sex hormones were determined by using ELISA. Results: At the end of 12 weeks of treatment, when the control group was compared with the moxa-smoke groups, the moxa-smoke groups exhibited the following results: sperm concentrations were significantly higher in groups M1 and M3 (p = 0.013), progressive motility was significantly higher in M1 (p = 0.006), and sperm motility was significantly higher in groups M1, M2 and M3 (M1, p = 0.003; M2, p = 0.044; M3, p = 0.008). Compared to the control group, TI was also significantly higher in groups M1, M2 and M3 (M1, p = 0.004; M2, p = 0.003; M3, p = 0.002), as were serum total testosterone (TT), free testosterone (FT) and testosterone secreting index (TSI), respectively (TT, p = 0.000; FT, TSI, p = 0.001). Compared to M1, TT was significantly lower in groups M2 and M3 (M2, p = 0.021; M3, P = 0.001). The comparison of LIN, WOB, STR, VCL, VSL and VAP between groups showed no significant differences. Conclusion: As compared to the non-moxa-smoke control group, low, middle and high concentrations of moxa smoke are effective to improve the male reproductive system in Wistar rats. More specifically, low moxa-smoke concentration seems slightly better than high concentrations for improving the sperm concentrations, progressive motility and sperm motility, TI, TT, FT, TSI of Wistar rats. © 2015.


Yu L.-Y.,Beijing Electrical Power Hospital | Pei Y.,Chinese PLA General Hospital
Chinese Medical Journal | Year: 2015

Objective: To analyze the mechanism of neuroprotection of insulin and which blood glucose range was benefit for insulin exerting neuroprotective action. Data Sources: The study is based on the data from PubMed. Study Selection: Articles were selected with the search terms “insulin”, “blood glucose”, “neuroprotection”, “brain”, “glycogen”, “cerebral ischemia”, “neuronal necrosis”, “glutamate”, “γ-aminobutyric acid”. Results: Insulin has neuroprotection. The mechanisms include the regulation of neurotransmitter, promoting glycogen synthesis, and inhibition of neuronal necrosis and apoptosis. Insulin could play its role in neuroprotection by avoiding hypoglycemia and hyperglycemia. Conclusions: Intermittent and long-term infusion insulin may be a benefit for patients with ischemic brain damage at blood glucose 6–9 mmol/L. © 2015, Chinese Medical Association. All rights reserved.

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