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Liu L.,CAS Institute of High Energy Physics | Li Y.,CAS Institute of High Energy Physics | Li T.,CAS Institute of High Energy Physics | Xie J.,CAS Institute of High Energy Physics | And 4 more authors.
Analytical Chemistry | Year: 2016

We have developed a nanopore sensing approach for the selective detection of 8-oxo-2′-deoxyguanosine (8-oxoG) in single-stranded DNA. First, 1,12-dodecanediamine is coupled with 8-oxoG-containing DNA molecules in high yield which leaves a free amine group for subsequent attaching of an adamantane moiety. After incubation with cucurbit[7]uril, the host-guest complex-modified DNA hybrid is translocated through an α-hemolysin nanopore. Highly characteristic events can be recorded and used to quantify the 8-oxoG-DNA content in a DNA mixture. Compared with the existing methods, this study provides a reliable, quick, and low-cost approach for the detection of 8-oxoG site in single-stranded DNA at the single-molecule level, particularly suitable for high-throughput screening of a massive number of samples. © 2015 American Chemical Society. Source


Chen T.-L.,Beijing Chaoyang Emergency Medical Center | Yong Y.-M.,Capital Medical University | Peng Y.-P.,Beijing Chaoyang Emergency Medical Center | Xie H.-F.,Beijing Chaoyang Emergency Medical Center | Jia W.D.,Beijing Chaoyang Emergency Medical Center
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: Bone cement solidification can improve the stability, strength and mechanical support of fractured vertebrae. However, there are few studies on the effect of bone cement dispersion within the fracture line on percutaneous vertebroplasty. OBJECTIVE: To analyze the effect of bone cement dispersion within the fracture line of thoracolumbar osteoporotic vertebral compression fractures on percutaneous vertebroplasty. METHODS: Totally 90 patients with thoracolumbar osteoporotic vertebral compression fractures were enrolled, 53-80 years old, including 42 males and 48 females. All these patients underwent percutaneous vertebroplasty with bone cement injection, and divided into two groups according to bone cement dispersion conditions: study group with good bone cement dispersion (n=60) and control group with poor bone cement dispersion (n=30). Visual analogue scale scores, Oswestry dysfunction index, Cobb’s angle and adverse reactions were recorded before and after treatment. RESULTS AND CONCLUSION: There were no differences in the visual analogue scale score and Oswestry dysfunction index between the two groups before treatment (P > 0. 05). The visual analogue scale scores were significantly lower in the study group than the control group at 3 days after treatment and at the last follow-up (P < 0. 05); the Oswestry dysfunction index and Cobb’s angle were also lower in the study group than the control group at 3 days after treatment (P < 0. 05). However, no difference was found in the Oswestry dysfunction index, Cobb’s angle and bone cement leakage between the two groups at the last follow-up. These findings indicate that the percutaneous vertebroplasty show better effects on pain relief in patients with good bone cement dispersion that those with poor bone cement dispersion, and the vertebral stability is better as well as the short-term effect is more obvious. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved. Source

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